8WA7

E.coli transketolase soaked with donor ketose D-fructose

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0004802	molecular_function	transketolase activity
A	0005829	cellular_component	cytosol
A	0006098	biological_process	pentose-phosphate shunt
A	0009052	biological_process	pentose-phosphate shunt, non-oxidative branch
A	0030145	molecular_function	manganese ion binding
A	0030976	molecular_function	thiamine pyrophosphate binding
A	0042803	molecular_function	protein homodimerization activity
B	0000287	molecular_function	magnesium ion binding
B	0004802	molecular_function	transketolase activity
B	0005829	cellular_component	cytosol
B	0006098	biological_process	pentose-phosphate shunt
B	0009052	biological_process	pentose-phosphate shunt, non-oxidative branch
B	0030145	molecular_function	manganese ion binding
B	0030976	molecular_function	thiamine pyrophosphate binding
B	0042803	molecular_function	protein homodimerization activity

Functional Information from PROSITE/UniProt

site_id	PS00801
Number of Residues	21
Details	TRANSKETOLASE_1 Transketolase signature 1. RalsMDavqkakSGHPGapMG

Chain	Residue	Details
A	ARG12-GLY32

---

site_id	PS00802
Number of Residues	17
Details	TRANSKETOLASE_2 Transketolase signature 2. GEDGPTHqPVEqvAslR

Chain	Residue	Details
A	GLY467-ARG483

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"32839604","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17914867","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32839604","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2R8O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6TJ9","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17914867","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27767080","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"APR-1999","submissionDatabase":"PDB data bank","title":"Crystal structure of Escherichia coli transketolase.","authors":["Isupov M.N.","Rupprecht M.P.","Wilson K.S.","Dauter Z.","Littlechild J.A."]}},{"source":"PDB","id":"1QGD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2R5N","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2R8O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2R8P","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5HHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17914867","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2R8O","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17914867","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27767080","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"APR-1999","submissionDatabase":"PDB data bank","title":"Crystal structure of Escherichia coli transketolase.","authors":["Isupov M.N.","Rupprecht M.P.","Wilson K.S.","Dauter Z.","Littlechild J.A."]}},{"source":"PubMed","id":"32839604","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1QGD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2R5N","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2R8O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2R8P","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5HHT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6TJ8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6TJ9","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27767080","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32839604","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"5HHT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6TJ8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6TJ9","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27767080","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"APR-1999","submissionDatabase":"PDB data bank","title":"Crystal structure of Escherichia coli transketolase.","authors":["Isupov M.N.","Rupprecht M.P.","Wilson K.S.","Dauter Z.","Littlechild J.A."]}},{"source":"PubMed","id":"32839604","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1QGD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5HHT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6TJ8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6TJ9","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"journal article","publicationDate":"2024","firstPage":"355","lastPage":"365","volume":"14","journal":"ACS Catal.","title":"Multifaceted Role of the Substrate Phosphate Group in Transketolase Catalysis.","authors":["Xiao C.","Lin S.","Tittmann K.","Dai S.","Liu Z."],"citationCrossReferences":[{"database":"DOI","id":"10.1021/acscatal.3c04543"}]}},{"source":"PubMed","id":"17914867","evidenceCode":"ECO:0000305"},{"source":"Reference","evidenceCode":"ECO:0000305","citation":{"citationType":"submission","publicationDate":"APR-1999","submissionDatabase":"PDB data bank","title":"Crystal structure of Escherichia coli transketolase.","authors":["Isupov M.N.","Rupprecht M.P.","Wilson K.S.","Dauter Z.","Littlechild J.A."]}},{"source":"PDB","id":"1QGD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2R5N","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8WA7","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"32839604","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6TJ9","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	Site: {"description":"Important for catalytic activity","evidences":[{"source":"PubMed","id":"17914867","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"18723842","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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