8W7D
Crystal structure of EcPPAT-FR901483 complex
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0004044 | molecular_function | amidophosphoribosyltransferase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006164 | biological_process | purine nucleotide biosynthetic process |
| A | 0006189 | biological_process | 'de novo' IMP biosynthetic process |
| A | 0006541 | biological_process | glutamine metabolic process |
| A | 0009113 | biological_process | purine nucleobase biosynthetic process |
| A | 0016757 | molecular_function | glycosyltransferase activity |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0097216 | molecular_function | guanosine tetraphosphate binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0004044 | molecular_function | amidophosphoribosyltransferase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006164 | biological_process | purine nucleotide biosynthetic process |
| B | 0006189 | biological_process | 'de novo' IMP biosynthetic process |
| B | 0006541 | biological_process | glutamine metabolic process |
| B | 0009113 | biological_process | purine nucleobase biosynthetic process |
| B | 0016757 | molecular_function | glycosyltransferase activity |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0097216 | molecular_function | guanosine tetraphosphate binding |
| C | 0000287 | molecular_function | magnesium ion binding |
| C | 0004044 | molecular_function | amidophosphoribosyltransferase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006164 | biological_process | purine nucleotide biosynthetic process |
| C | 0006189 | biological_process | 'de novo' IMP biosynthetic process |
| C | 0006541 | biological_process | glutamine metabolic process |
| C | 0009113 | biological_process | purine nucleobase biosynthetic process |
| C | 0016757 | molecular_function | glycosyltransferase activity |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0097216 | molecular_function | guanosine tetraphosphate binding |
| D | 0000287 | molecular_function | magnesium ion binding |
| D | 0004044 | molecular_function | amidophosphoribosyltransferase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006164 | biological_process | purine nucleotide biosynthetic process |
| D | 0006189 | biological_process | 'de novo' IMP biosynthetic process |
| D | 0006541 | biological_process | glutamine metabolic process |
| D | 0009113 | biological_process | purine nucleobase biosynthetic process |
| D | 0016757 | molecular_function | glycosyltransferase activity |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0097216 | molecular_function | guanosine tetraphosphate binding |
Functional Information from PROSITE/UniProt
| site_id | PS00103 |
| Number of Residues | 13 |
| Details | PUR_PYR_PR_TRANSFER Purine/pyrimidine phosphoribosyl transferases signature. VLLVDDSIVRGtT |
| Chain | Residue | Details |
| A | VAL363-THR375 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Nucleophile => ECO:0000255|HAMAP-Rule:MF_01931, ECO:0000269|PubMed:7037784 |
| Chain | Residue | Details |
| A | CYS2 | |
| B | CYS2 | |
| C | CYS2 | |
| D | CYS2 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01931 |
| Chain | Residue | Details |
| A | THR305 | |
| D | THR305 | |
| D | ASP367 | |
| D | ASP368 | |
| A | ASP367 | |
| A | ASP368 | |
| B | THR305 | |
| B | ASP367 | |
| B | ASP368 | |
| C | THR305 | |
| C | ASP367 | |
| C | ASP368 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 214 |
| Chain | Residue | Details |
| A | CYS2 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
| A | GLY28 | activator, electrostatic stabiliser, hydrogen bond acceptor |
| A | ASN102 | electrostatic stabiliser, hydrogen bond donor |
| A | GLY103 | electrostatic stabiliser, hydrogen bond donor |
| A | TYR259 | electrostatic stabiliser, hydrogen bond donor |
| site_id | MCSA2 |
| Number of Residues | 5 |
| Details | M-CSA 214 |
| Chain | Residue | Details |
| B | CYS2 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
| B | GLY28 | activator, electrostatic stabiliser, hydrogen bond acceptor |
| B | ASN102 | electrostatic stabiliser, hydrogen bond donor |
| B | GLY103 | electrostatic stabiliser, hydrogen bond donor |
| B | TYR259 | electrostatic stabiliser, hydrogen bond donor |
| site_id | MCSA3 |
| Number of Residues | 5 |
| Details | M-CSA 214 |
| Chain | Residue | Details |
| C | CYS2 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
| C | GLY28 | activator, electrostatic stabiliser, hydrogen bond acceptor |
| C | ASN102 | electrostatic stabiliser, hydrogen bond donor |
| C | GLY103 | electrostatic stabiliser, hydrogen bond donor |
| C | TYR259 | electrostatic stabiliser, hydrogen bond donor |
| site_id | MCSA4 |
| Number of Residues | 5 |
| Details | M-CSA 214 |
| Chain | Residue | Details |
| D | CYS2 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
| D | GLY28 | activator, electrostatic stabiliser, hydrogen bond acceptor |
| D | ASN102 | electrostatic stabiliser, hydrogen bond donor |
| D | GLY103 | electrostatic stabiliser, hydrogen bond donor |
| D | TYR259 | electrostatic stabiliser, hydrogen bond donor |
