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8W41

Cryo-EM structure of Myosin VI in the autoinhibited state

Functional Information from GO Data
ChainGOidnamespacecontents
A0000146molecular_functionmicrofilament motor activity
A0000166molecular_functionnucleotide binding
A0001726cellular_componentruffle
A0003774molecular_functioncytoskeletal motor activity
A0003779molecular_functionactin binding
A0005515molecular_functionprotein binding
A0005516molecular_functioncalmodulin binding
A0005524molecular_functionATP binding
A0005634cellular_componentnucleus
A0005654cellular_componentnucleoplasm
A0005737cellular_componentcytoplasm
A0005765cellular_componentlysosomal membrane
A0005768cellular_componentendosome
A0005776cellular_componentautophagosome
A0005794cellular_componentGolgi apparatus
A0005829cellular_componentcytosol
A0005884cellular_componentactin filament
A0005886cellular_componentplasma membrane
A0005902cellular_componentmicrovillus
A0005905cellular_componentclathrin-coated pit
A0005938cellular_componentcell cortex
A0006886biological_processintracellular protein transport
A0006897biological_processendocytosis
A0007015biological_processactin filament organization
A0007605biological_processsensory perception of sound
A0008104biological_processprotein localization
A0009410biological_processresponse to xenobiotic stimulus
A0015031biological_processprotein transport
A0015629cellular_componentactin cytoskeleton
A0016020cellular_componentmembrane
A0016459cellular_componentmyosin complex
A0016461cellular_componentunconventional myosin complex
A0030048biological_processactin filament-based movement
A0030136cellular_componentclathrin-coated vesicle
A0030139cellular_componentendocytic vesicle
A0030175cellular_componentfilopodium
A0030330biological_processDNA damage response, signal transduction by p53 class mediator
A0030665cellular_componentclathrin-coated vesicle membrane
A0031410cellular_componentcytoplasmic vesicle
A0031941cellular_componentfilamentous actin
A0031965cellular_componentnuclear membrane
A0032587cellular_componentruffle membrane
A0032991cellular_componentprotein-containing complex
A0042472biological_processinner ear morphogenesis
A0042491biological_processinner ear auditory receptor cell differentiation
A0042995cellular_componentcell projection
A0043531molecular_functionADP binding
A0045177cellular_componentapical part of cell
A0045334cellular_componentclathrin-coated endocytic vesicle
A0048471cellular_componentperinuclear region of cytoplasm
A0051015molecular_functionactin filament binding
A0051046biological_processregulation of secretion
A0060001molecular_functionminus-end directed microfilament motor activity
A0070062cellular_componentextracellular exosome
B0000086biological_processG2/M transition of mitotic cell cycle
B0000922cellular_componentspindle pole
B0001975biological_processresponse to amphetamine
B0002027biological_processregulation of heart rate
B0005246molecular_functioncalcium channel regulator activity
B0005509molecular_functioncalcium ion binding
B0005513biological_processdetection of calcium ion
B0005515molecular_functionprotein binding
B0005654cellular_componentnucleoplasm
B0005737cellular_componentcytoplasm
B0005739cellular_componentmitochondrion
B0005813cellular_componentcentrosome
B0005819cellular_componentspindle
B0005829cellular_componentcytosol
B0005856cellular_componentcytoskeleton
B0005876cellular_componentspindle microtubule
B0005929cellular_componentcilium
B0006897biological_processendocytosis
B0008076cellular_componentvoltage-gated potassium channel complex
B0008179molecular_functionadenylate cyclase binding
B0010856molecular_functionadenylate cyclase activator activity
B0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
B0010881biological_processregulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
B0016240biological_processautophagosome membrane docking
B0019855molecular_functioncalcium channel inhibitor activity
B0019901molecular_functionprotein kinase binding
B0019904molecular_functionprotein domain specific binding
B0030017cellular_componentsarcomere
B0030235molecular_functionnitric-oxide synthase regulator activity
B0030426cellular_componentgrowth cone
B0030672cellular_componentsynaptic vesicle membrane
B0031432molecular_functiontitin binding
B0031514cellular_componentmotile cilium
B0031800molecular_functiontype 3 metabotropic glutamate receptor binding
B0031966cellular_componentmitochondrial membrane
B0032465biological_processregulation of cytokinesis
B0032991cellular_componentprotein-containing complex
B0034704cellular_componentcalcium channel complex
B0035458biological_processcellular response to interferon-beta
B0042995cellular_componentcell projection
B0043209cellular_componentmyelin sheath
B0043539molecular_functionprotein serine/threonine kinase activator activity
B0043548molecular_functionphosphatidylinositol 3-kinase binding
B0044305cellular_componentcalyx of Held
B0044325molecular_functiontransmembrane transporter binding
B0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
B0046872molecular_functionmetal ion binding
B0048306molecular_functioncalcium-dependent protein binding
B0050998molecular_functionnitric-oxide synthase binding
B0051412biological_processresponse to corticosterone
B0051592biological_processresponse to calcium ion
B0051649biological_processestablishment of localization in cell
B0055117biological_processregulation of cardiac muscle contraction
B0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
B0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
B0071346biological_processcellular response to type II interferon
B0072542molecular_functionprotein phosphatase activator activity
B0090150biological_processestablishment of protein localization to membrane
B0090151biological_processestablishment of protein localization to mitochondrial membrane
B0097225cellular_componentsperm midpiece
B0097720biological_processcalcineurin-mediated signaling
B0098685cellular_componentSchaffer collateral - CA1 synapse
B0098901biological_processregulation of cardiac muscle cell action potential
B0099523cellular_componentpresynaptic cytosol
B0099524cellular_componentpostsynaptic cytosol
B0140056biological_processorganelle localization by membrane tethering
B0140238biological_processpresynaptic endocytosis
B0150034cellular_componentdistal axon
B1900242biological_processregulation of synaptic vesicle endocytosis
B1902494cellular_componentcatalytic complex
B1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
B2000300biological_processregulation of synaptic vesicle exocytosis
I0000086biological_processG2/M transition of mitotic cell cycle
I0000922cellular_componentspindle pole
I0001975biological_processresponse to amphetamine
I0002027biological_processregulation of heart rate
I0005246molecular_functioncalcium channel regulator activity
I0005509molecular_functioncalcium ion binding
I0005513biological_processdetection of calcium ion
I0005515molecular_functionprotein binding
I0005654cellular_componentnucleoplasm
I0005737cellular_componentcytoplasm
I0005739cellular_componentmitochondrion
I0005813cellular_componentcentrosome
I0005819cellular_componentspindle
I0005829cellular_componentcytosol
I0005856cellular_componentcytoskeleton
I0005876cellular_componentspindle microtubule
I0005929cellular_componentcilium
I0006897biological_processendocytosis
I0008076cellular_componentvoltage-gated potassium channel complex
I0008179molecular_functionadenylate cyclase binding
I0010856molecular_functionadenylate cyclase activator activity
I0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
I0010881biological_processregulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
I0016240biological_processautophagosome membrane docking
I0019855molecular_functioncalcium channel inhibitor activity
I0019901molecular_functionprotein kinase binding
I0019904molecular_functionprotein domain specific binding
I0030017cellular_componentsarcomere
I0030235molecular_functionnitric-oxide synthase regulator activity
I0030426cellular_componentgrowth cone
I0030672cellular_componentsynaptic vesicle membrane
I0031432molecular_functiontitin binding
I0031514cellular_componentmotile cilium
I0031800molecular_functiontype 3 metabotropic glutamate receptor binding
I0031966cellular_componentmitochondrial membrane
I0032465biological_processregulation of cytokinesis
I0032991cellular_componentprotein-containing complex
I0034704cellular_componentcalcium channel complex
I0035458biological_processcellular response to interferon-beta
I0042995cellular_componentcell projection
I0043209cellular_componentmyelin sheath
I0043539molecular_functionprotein serine/threonine kinase activator activity
I0043548molecular_functionphosphatidylinositol 3-kinase binding
I0044305cellular_componentcalyx of Held
I0044325molecular_functiontransmembrane transporter binding
I0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
I0046872molecular_functionmetal ion binding
I0048306molecular_functioncalcium-dependent protein binding
I0050998molecular_functionnitric-oxide synthase binding
I0051412biological_processresponse to corticosterone
I0051592biological_processresponse to calcium ion
I0051649biological_processestablishment of localization in cell
I0055117biological_processregulation of cardiac muscle contraction
I0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
I0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
I0071346biological_processcellular response to type II interferon
I0072542molecular_functionprotein phosphatase activator activity
I0090150biological_processestablishment of protein localization to membrane
I0090151biological_processestablishment of protein localization to mitochondrial membrane
I0097225cellular_componentsperm midpiece
I0097720biological_processcalcineurin-mediated signaling
I0098685cellular_componentSchaffer collateral - CA1 synapse
I0098901biological_processregulation of cardiac muscle cell action potential
I0099523cellular_componentpresynaptic cytosol
I0099524cellular_componentpostsynaptic cytosol
I0140056biological_processorganelle localization by membrane tethering
I0140238biological_processpresynaptic endocytosis
I0150034cellular_componentdistal axon
I1900242biological_processregulation of synaptic vesicle endocytosis
I1902494cellular_componentcatalytic complex
I1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
I2000300biological_processregulation of synaptic vesicle exocytosis
Functional Information from PROSITE/UniProt
site_idPS00018
Number of Residues13
DetailsEF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL
ChainResidueDetails
BASP21-LEU33
BASP57-PHE69
BASP94-LEU106
BASP130-PHE142

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues1
DetailsBINDING: BINDING => ECO:0000255
ChainResidueDetails
AGLY151
BGLU68
BASP94
BASP96
BASN98
BTYR100
BGLU105
BASP130
BASP132
BASP134
BGLN136
BASP23
BGLU141
IASP21
IASP23
IASP25
ITHR27
IGLU32
IASP57
IASP59
IASN61
ITHR63
BASP25
IGLU68
IASP94
IASP96
IASN98
ITYR100
IGLU105
IASP130
IASP132
IASP134
IGLN136
BTHR27
IGLU141
BGLU32
BASP57
BASP59
BASN61
BTHR63

site_idSWS_FT_FI2
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163
ChainResidueDetails
ASER267
IALA2

site_idSWS_FT_FI3
Number of Residues1
DetailsMOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569
ChainResidueDetails
ATHR405
ILYS22

site_idSWS_FT_FI4
Number of Residues3
DetailsMOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q64331
ChainResidueDetails
ASER604
ASER1025
AILE1155

site_idSWS_FT_FI5
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
BSER82
ISER82

site_idSWS_FT_FI6
Number of Residues2
DetailsMOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
BLYS95
ILYS95

site_idSWS_FT_FI7
Number of Residues2
DetailsMOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18034455, ECO:0007744|PubMed:21183079
ChainResidueDetails
BTYR100
ITYR100

site_idSWS_FT_FI8
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:21183079
ChainResidueDetails
BSER102
ISER102

site_idSWS_FT_FI9
Number of Residues2
DetailsMOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
BTHR111
ITHR111

site_idSWS_FT_FI10
Number of Residues2
DetailsMOD_RES: N6-methyllysine; alternate => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
BLYS116
ILYS116

site_idSWS_FT_FI11
Number of Residues2
DetailsMOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
BTYR139
ITYR139

site_idSWS_FT_FI12
Number of Residues4
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P62157
ChainResidueDetails
BLYS22
ILYS22

237423

PDB entries from 2025-06-11

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