8W2J

Human liver phosphofructokinase-1 filament in the T-state conformation

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003824	molecular_function	catalytic activity
A	0003872	molecular_function	6-phosphofructokinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005576	cellular_component	extracellular region
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005945	cellular_component	6-phosphofructokinase complex
A	0006002	biological_process	fructose 6-phosphate metabolic process
A	0006096	biological_process	glycolytic process
A	0009749	biological_process	response to glucose
A	0016020	cellular_component	membrane
A	0016301	molecular_function	kinase activity
A	0016740	molecular_function	transferase activity
A	0019900	molecular_function	kinase binding
A	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
A	0034774	cellular_component	secretory granule lumen
A	0042802	molecular_function	identical protein binding
A	0046676	biological_process	negative regulation of insulin secretion
A	0046872	molecular_function	metal ion binding
A	0048029	molecular_function	monosaccharide binding
A	0061615	biological_process	glycolytic process through fructose-6-phosphate
A	0061621	biological_process	canonical glycolysis
A	0070061	molecular_function	fructose binding
A	0070062	cellular_component	extracellular exosome
A	0070095	molecular_function	fructose-6-phosphate binding
A	1904813	cellular_component	ficolin-1-rich granule lumen
B	0000166	molecular_function	nucleotide binding
B	0003824	molecular_function	catalytic activity
B	0003872	molecular_function	6-phosphofructokinase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005576	cellular_component	extracellular region
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005945	cellular_component	6-phosphofructokinase complex
B	0006002	biological_process	fructose 6-phosphate metabolic process
B	0006096	biological_process	glycolytic process
B	0009749	biological_process	response to glucose
B	0016020	cellular_component	membrane
B	0016301	molecular_function	kinase activity
B	0016740	molecular_function	transferase activity
B	0019900	molecular_function	kinase binding
B	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
B	0034774	cellular_component	secretory granule lumen
B	0042802	molecular_function	identical protein binding
B	0046676	biological_process	negative regulation of insulin secretion
B	0046872	molecular_function	metal ion binding
B	0048029	molecular_function	monosaccharide binding
B	0061615	biological_process	glycolytic process through fructose-6-phosphate
B	0061621	biological_process	canonical glycolysis
B	0070061	molecular_function	fructose binding
B	0070062	cellular_component	extracellular exosome
B	0070095	molecular_function	fructose-6-phosphate binding
B	1904813	cellular_component	ficolin-1-rich granule lumen
C	0000166	molecular_function	nucleotide binding
C	0003824	molecular_function	catalytic activity
C	0003872	molecular_function	6-phosphofructokinase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005576	cellular_component	extracellular region
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005945	cellular_component	6-phosphofructokinase complex
C	0006002	biological_process	fructose 6-phosphate metabolic process
C	0006096	biological_process	glycolytic process
C	0009749	biological_process	response to glucose
C	0016020	cellular_component	membrane
C	0016301	molecular_function	kinase activity
C	0016740	molecular_function	transferase activity
C	0019900	molecular_function	kinase binding
C	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
C	0034774	cellular_component	secretory granule lumen
C	0042802	molecular_function	identical protein binding
C	0046676	biological_process	negative regulation of insulin secretion
C	0046872	molecular_function	metal ion binding
C	0048029	molecular_function	monosaccharide binding
C	0061615	biological_process	glycolytic process through fructose-6-phosphate
C	0061621	biological_process	canonical glycolysis
C	0070061	molecular_function	fructose binding
C	0070062	cellular_component	extracellular exosome
C	0070095	molecular_function	fructose-6-phosphate binding
C	1904813	cellular_component	ficolin-1-rich granule lumen
D	0000166	molecular_function	nucleotide binding
D	0003824	molecular_function	catalytic activity
D	0003872	molecular_function	6-phosphofructokinase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005576	cellular_component	extracellular region
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005945	cellular_component	6-phosphofructokinase complex
D	0006002	biological_process	fructose 6-phosphate metabolic process
D	0006096	biological_process	glycolytic process
D	0009749	biological_process	response to glucose
D	0016020	cellular_component	membrane
D	0016301	molecular_function	kinase activity
D	0016740	molecular_function	transferase activity
D	0019900	molecular_function	kinase binding
D	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
D	0034774	cellular_component	secretory granule lumen
D	0042802	molecular_function	identical protein binding
D	0046676	biological_process	negative regulation of insulin secretion
D	0046872	molecular_function	metal ion binding
D	0048029	molecular_function	monosaccharide binding
D	0061615	biological_process	glycolytic process through fructose-6-phosphate
D	0061621	biological_process	canonical glycolysis
D	0070061	molecular_function	fructose binding
D	0070062	cellular_component	extracellular exosome
D	0070095	molecular_function	fructose-6-phosphate binding
D	1904813	cellular_component	ficolin-1-rich granule lumen
E	0000166	molecular_function	nucleotide binding
E	0003824	molecular_function	catalytic activity
E	0003872	molecular_function	6-phosphofructokinase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005576	cellular_component	extracellular region
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005945	cellular_component	6-phosphofructokinase complex
E	0006002	biological_process	fructose 6-phosphate metabolic process
E	0006096	biological_process	glycolytic process
E	0009749	biological_process	response to glucose
E	0016020	cellular_component	membrane
E	0016301	molecular_function	kinase activity
E	0016740	molecular_function	transferase activity
E	0019900	molecular_function	kinase binding
E	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
E	0034774	cellular_component	secretory granule lumen
E	0042802	molecular_function	identical protein binding
E	0046676	biological_process	negative regulation of insulin secretion
E	0046872	molecular_function	metal ion binding
E	0048029	molecular_function	monosaccharide binding
E	0061615	biological_process	glycolytic process through fructose-6-phosphate
E	0061621	biological_process	canonical glycolysis
E	0070061	molecular_function	fructose binding
E	0070062	cellular_component	extracellular exosome
E	0070095	molecular_function	fructose-6-phosphate binding
E	1904813	cellular_component	ficolin-1-rich granule lumen
F	0000166	molecular_function	nucleotide binding
F	0003824	molecular_function	catalytic activity
F	0003872	molecular_function	6-phosphofructokinase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005576	cellular_component	extracellular region
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005945	cellular_component	6-phosphofructokinase complex
F	0006002	biological_process	fructose 6-phosphate metabolic process
F	0006096	biological_process	glycolytic process
F	0009749	biological_process	response to glucose
F	0016020	cellular_component	membrane
F	0016301	molecular_function	kinase activity
F	0016740	molecular_function	transferase activity
F	0019900	molecular_function	kinase binding
F	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
F	0034774	cellular_component	secretory granule lumen
F	0042802	molecular_function	identical protein binding
F	0046676	biological_process	negative regulation of insulin secretion
F	0046872	molecular_function	metal ion binding
F	0048029	molecular_function	monosaccharide binding
F	0061615	biological_process	glycolytic process through fructose-6-phosphate
F	0061621	biological_process	canonical glycolysis
F	0070061	molecular_function	fructose binding
F	0070062	cellular_component	extracellular exosome
F	0070095	molecular_function	fructose-6-phosphate binding
F	1904813	cellular_component	ficolin-1-rich granule lumen
G	0000166	molecular_function	nucleotide binding
G	0003824	molecular_function	catalytic activity
G	0003872	molecular_function	6-phosphofructokinase activity
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005576	cellular_component	extracellular region
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005945	cellular_component	6-phosphofructokinase complex
G	0006002	biological_process	fructose 6-phosphate metabolic process
G	0006096	biological_process	glycolytic process
G	0009749	biological_process	response to glucose
G	0016020	cellular_component	membrane
G	0016301	molecular_function	kinase activity
G	0016740	molecular_function	transferase activity
G	0019900	molecular_function	kinase binding
G	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
G	0034774	cellular_component	secretory granule lumen
G	0042802	molecular_function	identical protein binding
G	0046676	biological_process	negative regulation of insulin secretion
G	0046872	molecular_function	metal ion binding
G	0048029	molecular_function	monosaccharide binding
G	0061615	biological_process	glycolytic process through fructose-6-phosphate
G	0061621	biological_process	canonical glycolysis
G	0070061	molecular_function	fructose binding
G	0070062	cellular_component	extracellular exosome
G	0070095	molecular_function	fructose-6-phosphate binding
G	1904813	cellular_component	ficolin-1-rich granule lumen
H	0000166	molecular_function	nucleotide binding
H	0003824	molecular_function	catalytic activity
H	0003872	molecular_function	6-phosphofructokinase activity
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005576	cellular_component	extracellular region
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005945	cellular_component	6-phosphofructokinase complex
H	0006002	biological_process	fructose 6-phosphate metabolic process
H	0006096	biological_process	glycolytic process
H	0009749	biological_process	response to glucose
H	0016020	cellular_component	membrane
H	0016301	molecular_function	kinase activity
H	0016740	molecular_function	transferase activity
H	0019900	molecular_function	kinase binding
H	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
H	0034774	cellular_component	secretory granule lumen
H	0042802	molecular_function	identical protein binding
H	0046676	biological_process	negative regulation of insulin secretion
H	0046872	molecular_function	metal ion binding
H	0048029	molecular_function	monosaccharide binding
H	0061615	biological_process	glycolytic process through fructose-6-phosphate
H	0061621	biological_process	canonical glycolysis
H	0070061	molecular_function	fructose binding
H	0070062	cellular_component	extracellular exosome
H	0070095	molecular_function	fructose-6-phosphate binding
H	1904813	cellular_component	ficolin-1-rich granule lumen

Functional Information from PROSITE/UniProt

site_id	PS00433
Number of Residues	19
Details	PHOSPHOFRUCTOKINASE Phosphofructokinase signature. RvtvlGHvQRGGtpsafDR

Chain	Residue	Details
E	ARG292-ARG310
E	ARG654-ARG672

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	72
Details	Region: {"description":"Interdomain linker"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_03184","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	80
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_03184","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	160
Details	Binding site: {"description":"in other chain","evidences":[{"source":"HAMAP-Rule","id":"MF_03184","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P47857","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"P12382","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	8
Details	Glycosylation: {"description":"O-linked (GlcNAc) serine","evidences":[{"source":"PubMed","id":"22923583","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---