8V2Z

Cryo-EM Structure of Smooth Muscle Gamma Actin (ACTG2) Mutant R257C

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005524	molecular_function	ATP binding
A	0005615	cellular_component	extracellular space
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005856	cellular_component	cytoskeleton
A	0010628	biological_process	positive regulation of gene expression
A	0016787	molecular_function	hydrolase activity
A	0030027	cellular_component	lamellipodium
A	0030175	cellular_component	filopodium
A	0032982	cellular_component	myosin filament
A	0044297	cellular_component	cell body
A	0070062	cellular_component	extracellular exosome
A	0071944	cellular_component	cell periphery
A	0072562	cellular_component	blood microparticle
A	0090131	biological_process	mesenchyme migration
B	0005524	molecular_function	ATP binding
B	0005615	cellular_component	extracellular space
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005856	cellular_component	cytoskeleton
B	0010628	biological_process	positive regulation of gene expression
B	0016787	molecular_function	hydrolase activity
B	0030027	cellular_component	lamellipodium
B	0030175	cellular_component	filopodium
B	0032982	cellular_component	myosin filament
B	0044297	cellular_component	cell body
B	0070062	cellular_component	extracellular exosome
B	0071944	cellular_component	cell periphery
B	0072562	cellular_component	blood microparticle
B	0090131	biological_process	mesenchyme migration
C	0005524	molecular_function	ATP binding
C	0005615	cellular_component	extracellular space
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0010628	biological_process	positive regulation of gene expression
C	0016787	molecular_function	hydrolase activity
C	0030027	cellular_component	lamellipodium
C	0030175	cellular_component	filopodium
C	0032982	cellular_component	myosin filament
C	0044297	cellular_component	cell body
C	0070062	cellular_component	extracellular exosome
C	0071944	cellular_component	cell periphery
C	0072562	cellular_component	blood microparticle
C	0090131	biological_process	mesenchyme migration
D	0005524	molecular_function	ATP binding
D	0005615	cellular_component	extracellular space
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005856	cellular_component	cytoskeleton
D	0010628	biological_process	positive regulation of gene expression
D	0016787	molecular_function	hydrolase activity
D	0030027	cellular_component	lamellipodium
D	0030175	cellular_component	filopodium
D	0032982	cellular_component	myosin filament
D	0044297	cellular_component	cell body
D	0070062	cellular_component	extracellular exosome
D	0071944	cellular_component	cell periphery
D	0072562	cellular_component	blood microparticle
D	0090131	biological_process	mesenchyme migration
E	0005524	molecular_function	ATP binding
E	0005615	cellular_component	extracellular space
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0010628	biological_process	positive regulation of gene expression
E	0016787	molecular_function	hydrolase activity
E	0030027	cellular_component	lamellipodium
E	0030175	cellular_component	filopodium
E	0032982	cellular_component	myosin filament
E	0044297	cellular_component	cell body
E	0070062	cellular_component	extracellular exosome
E	0071944	cellular_component	cell periphery
E	0072562	cellular_component	blood microparticle
E	0090131	biological_process	mesenchyme migration

Functional Information from PROSITE/UniProt

site_id	PS00406
Number of Residues	11
Details	ACTINS_1 Actins signature 1. YVGDEAQs.KRG

Chain	Residue	Details
A	TYR53-GLY63

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site_id	PS00432
Number of Residues	9
Details	ACTINS_2 Actins signature 2. WISKpEYDE

Chain	Residue	Details
A	TRP356-GLU364

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site_id	PS01132
Number of Residues	13
Details	ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR

Chain	Residue	Details
A	LEU104-ARG116

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	5
Details	MOD_RES: N-acetylcysteine; in intermediate form => ECO:0000250|UniProtKB:P62737

Chain	Residue	Details
A	CYS1
B	CYS1
C	CYS1
D	CYS1
E	CYS1

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site_id	SWS_FT_FI2
Number of Residues	5
Details	MOD_RES: N-acetylglutamate; in Actin, gamma-enteric smooth muscle => ECO:0007744|PubMed:22223895

Chain	Residue	Details
A	GLU2
B	GLU2
C	GLU2
D	GLU2
E	GLU2

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site_id	SWS_FT_FI3
Number of Residues	10
Details	MOD_RES: Methionine (R)-sulfoxide => ECO:0000250|UniProtKB:P63268

Chain	Residue	Details
A	MET44
E	MET47
A	MET47
B	MET44
B	MET47
C	MET44
C	MET47
D	MET44
D	MET47
E	MET44

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site_id	SWS_FT_FI4
Number of Residues	5
Details	MOD_RES: Tele-methylhistidine => ECO:0000269|PubMed:30626964, ECO:0000269|PubMed:31993215

Chain	Residue	Details
A	HIC73
B	HIC73
C	HIC73
D	HIC73
E	HIC73

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site_id	SWS_FT_FI5
Number of Residues	5
Details	CROSSLNK: Isoglutamyl lysine isopeptide (Lys-Glu) (interchain with E-271); by Vibrio toxins RtxA and VgrG1 => ECO:0000250|UniProtKB:P60709

Chain	Residue	Details
A	LYS50
B	LYS50
C	LYS50
D	LYS50
E	LYS50

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site_id	SWS_FT_FI6
Number of Residues	10
Details	CROSSLNK: Isoglutamyl lysine isopeptide (Glu-Lys) (interchain with K-51); by Vibrio toxins RtxA and VgrG1 => ECO:0000250|UniProtKB:P60709

Chain	Residue	Details
E	GLU270
A	GLU270
B	GLU270
C	GLU270
D	GLU270

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