8UK2

The rotavirus VP5*/VP8* conformational transition permeabilizes membranes to Ca2+ (class 5 reconstruction)

Functional Information from GO Data

Chain	GOid	namespace	contents
1	0016020	cellular_component	membrane
1	0019028	cellular_component	viral capsid
1	0019062	biological_process	virion attachment to host cell
1	0020002	cellular_component	host cell plasma membrane
1	0030430	cellular_component	host cell cytoplasm
1	0039624	cellular_component	viral outer capsid
1	0039665	biological_process	permeabilization of host organelle membrane involved in viral entry into host cell
1	0044163	cellular_component	host cytoskeleton
1	0044165	cellular_component	host cell endoplasmic reticulum
1	0044168	cellular_component	host cell rough endoplasmic reticulum
1	0044172	cellular_component	host cell endoplasmic reticulum-Golgi intermediate compartment
1	0046718	biological_process	symbiont entry into host cell
1	0099008	biological_process	symbiont entry into host cell via permeabilization of inner membrane
2	0016020	cellular_component	membrane
2	0019028	cellular_component	viral capsid
2	0019062	biological_process	virion attachment to host cell
2	0020002	cellular_component	host cell plasma membrane
2	0030430	cellular_component	host cell cytoplasm
2	0039624	cellular_component	viral outer capsid
2	0039665	biological_process	permeabilization of host organelle membrane involved in viral entry into host cell
2	0044163	cellular_component	host cytoskeleton
2	0044165	cellular_component	host cell endoplasmic reticulum
2	0044168	cellular_component	host cell rough endoplasmic reticulum
2	0044172	cellular_component	host cell endoplasmic reticulum-Golgi intermediate compartment
2	0046718	biological_process	symbiont entry into host cell
2	0099008	biological_process	symbiont entry into host cell via permeabilization of inner membrane
3	0016020	cellular_component	membrane
3	0019028	cellular_component	viral capsid
3	0019062	biological_process	virion attachment to host cell
3	0020002	cellular_component	host cell plasma membrane
3	0030430	cellular_component	host cell cytoplasm
3	0039624	cellular_component	viral outer capsid
3	0039665	biological_process	permeabilization of host organelle membrane involved in viral entry into host cell
3	0044163	cellular_component	host cytoskeleton
3	0044165	cellular_component	host cell endoplasmic reticulum
3	0044168	cellular_component	host cell rough endoplasmic reticulum
3	0044172	cellular_component	host cell endoplasmic reticulum-Golgi intermediate compartment
3	0046718	biological_process	symbiont entry into host cell
3	0099008	biological_process	symbiont entry into host cell via permeabilization of inner membrane

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	SITE: Binding to sialic acid => ECO:0000255|HAMAP-Rule:MF_04132, ECO:0000269|PubMed:18974199

Chain	Residue	Details
1	ARG101
g	ASP228
d	ASP228
d	VAL229
d	ASP301
e	ASP95
e	GLN177
e	GLY206
e	ASP228
e	VAL229
e	ASP301
g	VAL229
g	ASP301
h	ASP95
h	GLN177
h	GLY206
h	ASP228
h	VAL229
h	ASP301
i	ASP95
1	SER190
i	GLN177
i	GLY206
i	ASP228
i	VAL229
i	ASP301
j	ASP95
j	GLN177
j	GLY206
j	ASP228
j	VAL229
2	ARG101
j	ASP301
k	ASP95
k	GLN177
k	GLY206
k	ASP228
k	VAL229
k	ASP301
l	ASP95
l	GLN177
l	GLY206
2	SER190
l	ASP228
l	VAL229
l	ASP301
m	ASP95
m	GLN177
m	GLY206
m	ASP228
m	VAL229
m	ASP301
n	ASP95
3	ARG101
n	GLN177
n	GLY206
n	ASP228
n	VAL229
n	ASP301
o	ASP95
o	GLN177
o	GLY206
o	ASP228
o	VAL229
3	SER190
o	ASP301
p	ASP95
p	GLN177
p	GLY206
p	ASP228
p	VAL229
p	ASP301
q	ASP95
q	GLN177
q	GLY206
g	ASP95
q	ASP228
q	VAL229
q	ASP301
r	ASP95
r	GLN177
r	GLY206
r	ASP228
r	VAL229
r	ASP301
a	ASP95
g	GLN177
a	GLN177
a	GLY206
a	ASP228
a	VAL229
a	ASP301
b	ASP95
b	GLN177
b	GLY206
b	ASP228
b	VAL229
g	GLY206
b	ASP301
c	ASP95
c	GLN177
c	GLY206
c	ASP228
c	VAL229
c	ASP301
d	ASP95
d	GLN177
d	GLY206

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	SITE: Cleavage => ECO:0000255|HAMAP-Rule:MF_04132, ECO:0000269|PubMed:8709201

Chain	Residue	Details
1	ARG231
i	THR214
i	GLU216
i	ASP231
j	THR214
j	GLU216
j	ASP231
k	THR214
k	GLU216
k	ASP231
l	THR214
1	ARG241
l	GLU216
l	ASP231
m	THR214
m	GLU216
m	ASP231
n	THR214
n	GLU216
n	ASP231
o	THR214
o	GLU216
2	ARG231
o	ASP231
p	THR214
p	GLU216
p	ASP231
q	THR214
q	GLU216
q	ASP231
r	THR214
r	GLU216
r	ASP231
2	ARG241
a	THR214
a	GLU216
a	ASP231
b	THR214
b	GLU216
b	ASP231
c	THR214
c	GLU216
c	ASP231
d	THR214
3	ARG231
d	GLU216
d	ASP231
e	THR214
e	GLU216
e	ASP231
3	ARG241
h	THR214
h	GLU216
h	ASP231

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	SITE: Cleavage; associated with enhancement of infectivity => ECO:0000255|HAMAP-Rule:MF_04132, ECO:0000269|PubMed:8709201, ECO:0000269|PubMed:9573313

Chain	Residue	Details
1	ARG247
o	ASN69
p	ASN69
q	ASN69
r	ASN69
a	ASN69
b	ASN69
c	ASN69
d	ASN69
e	ASN69
2	ARG247
3	ARG247
i	ASN69
j	ASN69
k	ASN69
l	ASN69
m	ASN69
n	ASN69

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