8TOW

Structure of a mutated photosystem II complex reveals perturbation of the oxygen-evolving complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005506	molecular_function	iron ion binding
A	0009055	molecular_function	electron transfer activity
A	0009523	cellular_component	photosystem II
A	0009579	cellular_component	thylakoid
A	0009635	biological_process	response to herbicide
A	0010242	molecular_function	oxygen evolving activity
A	0015979	biological_process	photosynthesis
A	0016020	cellular_component	membrane
A	0016168	molecular_function	chlorophyll binding
A	0016491	molecular_function	oxidoreductase activity
A	0016682	molecular_function	oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor
A	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
A	0031676	cellular_component	plasma membrane-derived thylakoid membrane
A	0042651	cellular_component	thylakoid membrane
A	0046872	molecular_function	metal ion binding
B	0009523	cellular_component	photosystem II
B	0009579	cellular_component	thylakoid
B	0015979	biological_process	photosynthesis
B	0016020	cellular_component	membrane
B	0016168	molecular_function	chlorophyll binding
B	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
B	0031676	cellular_component	plasma membrane-derived thylakoid membrane
B	0042651	cellular_component	thylakoid membrane
C	0005737	cellular_component	cytoplasm
C	0009523	cellular_component	photosystem II
C	0009579	cellular_component	thylakoid
C	0015979	biological_process	photosynthesis
C	0016020	cellular_component	membrane
C	0016168	molecular_function	chlorophyll binding
C	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
C	0031676	cellular_component	plasma membrane-derived thylakoid membrane
C	0042651	cellular_component	thylakoid membrane
C	0046872	molecular_function	metal ion binding
D	0005506	molecular_function	iron ion binding
D	0009055	molecular_function	electron transfer activity
D	0009523	cellular_component	photosystem II
D	0009579	cellular_component	thylakoid
D	0010242	molecular_function	oxygen evolving activity
D	0015979	biological_process	photosynthesis
D	0016020	cellular_component	membrane
D	0016168	molecular_function	chlorophyll binding
D	0016491	molecular_function	oxidoreductase activity
D	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
D	0031676	cellular_component	plasma membrane-derived thylakoid membrane
D	0042651	cellular_component	thylakoid membrane
D	0046872	molecular_function	metal ion binding
E	0005506	molecular_function	iron ion binding
E	0005737	cellular_component	cytoplasm
E	0009055	molecular_function	electron transfer activity
E	0009523	cellular_component	photosystem II
E	0009579	cellular_component	thylakoid
E	0015979	biological_process	photosynthesis
E	0016020	cellular_component	membrane
E	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
E	0031676	cellular_component	plasma membrane-derived thylakoid membrane
E	0042651	cellular_component	thylakoid membrane
E	0046872	molecular_function	metal ion binding
F	0005506	molecular_function	iron ion binding
F	0005737	cellular_component	cytoplasm
F	0009055	molecular_function	electron transfer activity
F	0009523	cellular_component	photosystem II
F	0009579	cellular_component	thylakoid
F	0015979	biological_process	photosynthesis
F	0016020	cellular_component	membrane
F	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
F	0031676	cellular_component	plasma membrane-derived thylakoid membrane
F	0042651	cellular_component	thylakoid membrane
F	0042802	molecular_function	identical protein binding
F	0046872	molecular_function	metal ion binding
H	0009523	cellular_component	photosystem II
H	0009579	cellular_component	thylakoid
H	0015979	biological_process	photosynthesis
H	0016020	cellular_component	membrane
H	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
H	0031676	cellular_component	plasma membrane-derived thylakoid membrane
H	0042651	cellular_component	thylakoid membrane
I	0005737	cellular_component	cytoplasm
I	0009523	cellular_component	photosystem II
I	0009579	cellular_component	thylakoid
I	0015979	biological_process	photosynthesis
I	0016020	cellular_component	membrane
I	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
I	0031676	cellular_component	plasma membrane-derived thylakoid membrane
I	0042651	cellular_component	thylakoid membrane
J	0009523	cellular_component	photosystem II
J	0009579	cellular_component	thylakoid
J	0015979	biological_process	photosynthesis
J	0016020	cellular_component	membrane
J	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
J	0031676	cellular_component	plasma membrane-derived thylakoid membrane
J	0042651	cellular_component	thylakoid membrane
K	0005737	cellular_component	cytoplasm
K	0009523	cellular_component	photosystem II
K	0009579	cellular_component	thylakoid
K	0015979	biological_process	photosynthesis
K	0016020	cellular_component	membrane
K	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
K	0031676	cellular_component	plasma membrane-derived thylakoid membrane
K	0042651	cellular_component	thylakoid membrane
L	0005737	cellular_component	cytoplasm
L	0009523	cellular_component	photosystem II
L	0009579	cellular_component	thylakoid
L	0015979	biological_process	photosynthesis
L	0016020	cellular_component	membrane
L	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
L	0031676	cellular_component	plasma membrane-derived thylakoid membrane
L	0042651	cellular_component	thylakoid membrane
M	0005737	cellular_component	cytoplasm
M	0009523	cellular_component	photosystem II
M	0009579	cellular_component	thylakoid
M	0015979	biological_process	photosynthesis
M	0016020	cellular_component	membrane
M	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
M	0031676	cellular_component	plasma membrane-derived thylakoid membrane
M	0042651	cellular_component	thylakoid membrane
R	0009523	cellular_component	photosystem II
R	0009579	cellular_component	thylakoid
R	0015979	biological_process	photosynthesis
R	0016020	cellular_component	membrane
R	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
R	0031676	cellular_component	plasma membrane-derived thylakoid membrane
R	0042651	cellular_component	thylakoid membrane
T	0005737	cellular_component	cytoplasm
T	0009523	cellular_component	photosystem II
T	0009579	cellular_component	thylakoid
T	0015979	biological_process	photosynthesis
T	0016020	cellular_component	membrane
T	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
T	0031676	cellular_component	plasma membrane-derived thylakoid membrane
T	0042651	cellular_component	thylakoid membrane
X	0009523	cellular_component	photosystem II
X	0009579	cellular_component	thylakoid
X	0015979	biological_process	photosynthesis
X	0016020	cellular_component	membrane
X	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
X	0031676	cellular_component	plasma membrane-derived thylakoid membrane
X	0042651	cellular_component	thylakoid membrane
Y	0009523	cellular_component	photosystem II
Y	0009579	cellular_component	thylakoid
Y	0015979	biological_process	photosynthesis
Y	0016020	cellular_component	membrane
Y	0031676	cellular_component	plasma membrane-derived thylakoid membrane
Y	0042651	cellular_component	thylakoid membrane
Z	0009523	cellular_component	photosystem II
Z	0009579	cellular_component	thylakoid
Z	0015979	biological_process	photosynthesis
Z	0016020	cellular_component	membrane
Z	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
Z	0031676	cellular_component	plasma membrane-derived thylakoid membrane
Z	0042651	cellular_component	thylakoid membrane
a	0005506	molecular_function	iron ion binding
a	0009055	molecular_function	electron transfer activity
a	0009523	cellular_component	photosystem II
a	0009579	cellular_component	thylakoid
a	0009635	biological_process	response to herbicide
a	0010242	molecular_function	oxygen evolving activity
a	0015979	biological_process	photosynthesis
a	0016020	cellular_component	membrane
a	0016168	molecular_function	chlorophyll binding
a	0016491	molecular_function	oxidoreductase activity
a	0016682	molecular_function	oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor
a	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
a	0031676	cellular_component	plasma membrane-derived thylakoid membrane
a	0042651	cellular_component	thylakoid membrane
a	0046872	molecular_function	metal ion binding
b	0009523	cellular_component	photosystem II
b	0009579	cellular_component	thylakoid
b	0015979	biological_process	photosynthesis
b	0016020	cellular_component	membrane
b	0016168	molecular_function	chlorophyll binding
b	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
b	0031676	cellular_component	plasma membrane-derived thylakoid membrane
b	0042651	cellular_component	thylakoid membrane
c	0005737	cellular_component	cytoplasm
c	0009523	cellular_component	photosystem II
c	0009579	cellular_component	thylakoid
c	0015979	biological_process	photosynthesis
c	0016020	cellular_component	membrane
c	0016168	molecular_function	chlorophyll binding
c	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
c	0031676	cellular_component	plasma membrane-derived thylakoid membrane
c	0042651	cellular_component	thylakoid membrane
c	0046872	molecular_function	metal ion binding
d	0005506	molecular_function	iron ion binding
d	0009055	molecular_function	electron transfer activity
d	0009523	cellular_component	photosystem II
d	0009579	cellular_component	thylakoid
d	0010242	molecular_function	oxygen evolving activity
d	0015979	biological_process	photosynthesis
d	0016020	cellular_component	membrane
d	0016168	molecular_function	chlorophyll binding
d	0016491	molecular_function	oxidoreductase activity
d	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
d	0031676	cellular_component	plasma membrane-derived thylakoid membrane
d	0042651	cellular_component	thylakoid membrane
d	0046872	molecular_function	metal ion binding
e	0005506	molecular_function	iron ion binding
e	0005737	cellular_component	cytoplasm
e	0009055	molecular_function	electron transfer activity
e	0009523	cellular_component	photosystem II
e	0009579	cellular_component	thylakoid
e	0015979	biological_process	photosynthesis
e	0016020	cellular_component	membrane
e	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
e	0031676	cellular_component	plasma membrane-derived thylakoid membrane
e	0042651	cellular_component	thylakoid membrane
e	0046872	molecular_function	metal ion binding
f	0005506	molecular_function	iron ion binding
f	0005737	cellular_component	cytoplasm
f	0009055	molecular_function	electron transfer activity
f	0009523	cellular_component	photosystem II
f	0009579	cellular_component	thylakoid
f	0015979	biological_process	photosynthesis
f	0016020	cellular_component	membrane
f	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
f	0031676	cellular_component	plasma membrane-derived thylakoid membrane
f	0042651	cellular_component	thylakoid membrane
f	0042802	molecular_function	identical protein binding
f	0046872	molecular_function	metal ion binding
h	0009523	cellular_component	photosystem II
h	0009579	cellular_component	thylakoid
h	0015979	biological_process	photosynthesis
h	0016020	cellular_component	membrane
h	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
h	0031676	cellular_component	plasma membrane-derived thylakoid membrane
h	0042651	cellular_component	thylakoid membrane
i	0005737	cellular_component	cytoplasm
i	0009523	cellular_component	photosystem II
i	0009579	cellular_component	thylakoid
i	0015979	biological_process	photosynthesis
i	0016020	cellular_component	membrane
i	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
i	0031676	cellular_component	plasma membrane-derived thylakoid membrane
i	0042651	cellular_component	thylakoid membrane
j	0009523	cellular_component	photosystem II
j	0009579	cellular_component	thylakoid
j	0015979	biological_process	photosynthesis
j	0016020	cellular_component	membrane
j	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
j	0031676	cellular_component	plasma membrane-derived thylakoid membrane
j	0042651	cellular_component	thylakoid membrane
k	0005737	cellular_component	cytoplasm
k	0009523	cellular_component	photosystem II
k	0009579	cellular_component	thylakoid
k	0015979	biological_process	photosynthesis
k	0016020	cellular_component	membrane
k	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
k	0031676	cellular_component	plasma membrane-derived thylakoid membrane
k	0042651	cellular_component	thylakoid membrane
l	0005737	cellular_component	cytoplasm
l	0009523	cellular_component	photosystem II
l	0009579	cellular_component	thylakoid
l	0015979	biological_process	photosynthesis
l	0016020	cellular_component	membrane
l	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
l	0031676	cellular_component	plasma membrane-derived thylakoid membrane
l	0042651	cellular_component	thylakoid membrane
m	0005737	cellular_component	cytoplasm
m	0009523	cellular_component	photosystem II
m	0009579	cellular_component	thylakoid
m	0015979	biological_process	photosynthesis
m	0016020	cellular_component	membrane
m	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
m	0031676	cellular_component	plasma membrane-derived thylakoid membrane
m	0042651	cellular_component	thylakoid membrane
r	0009523	cellular_component	photosystem II
r	0009579	cellular_component	thylakoid
r	0015979	biological_process	photosynthesis
r	0016020	cellular_component	membrane
r	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
r	0031676	cellular_component	plasma membrane-derived thylakoid membrane
r	0042651	cellular_component	thylakoid membrane
t	0005737	cellular_component	cytoplasm
t	0009523	cellular_component	photosystem II
t	0009579	cellular_component	thylakoid
t	0015979	biological_process	photosynthesis
t	0016020	cellular_component	membrane
t	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
t	0031676	cellular_component	plasma membrane-derived thylakoid membrane
t	0042651	cellular_component	thylakoid membrane
x	0009523	cellular_component	photosystem II
x	0009579	cellular_component	thylakoid
x	0015979	biological_process	photosynthesis
x	0016020	cellular_component	membrane
x	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
x	0031676	cellular_component	plasma membrane-derived thylakoid membrane
x	0042651	cellular_component	thylakoid membrane
y	0009523	cellular_component	photosystem II
y	0009579	cellular_component	thylakoid
y	0015979	biological_process	photosynthesis
y	0016020	cellular_component	membrane
y	0031676	cellular_component	plasma membrane-derived thylakoid membrane
y	0042651	cellular_component	thylakoid membrane
z	0009523	cellular_component	photosystem II
z	0009579	cellular_component	thylakoid
z	0015979	biological_process	photosynthesis
z	0016020	cellular_component	membrane
z	0030096	cellular_component	plasma membrane-derived thylakoid photosystem II
z	0031676	cellular_component	plasma membrane-derived thylakoid membrane
z	0042651	cellular_component	thylakoid membrane

Functional Information from PROSITE/UniProt

site_id	PS00244
Number of Residues	27
Details	REACTION_CENTER Photosynthetic reaction center proteins signature. NwtlnPfHmmGvagilggallcAiHGA

Chain	Residue	Details
D	ASN190-ALA216
A	ASN191-SER217

---

site_id	PS00537
Number of Residues	15
Details	CYTOCHROME_B559 Cytochrome b559 subunits heme-binding site signature. VtSIRYwvIHSITIP

Chain	Residue	Details
E	VAL14-PRO28
f	ILE14-PRO28

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	86
Details	TRANSMEM: Helical => ECO:0000269|PubMed:34937700, ECO:0000312|PDB:7N8O

Chain	Residue	Details
Z	PHE5-TYR27
Z	PRO38-PHE59
z	PHE5-TYR27
z	PRO38-PHE59
d	ILE132-PRO140
d	VAL218-ARG265
c	THR241-GLY255
c	ALA437-ASP460

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: axial binding residue => ECO:0000269|PubMed:11315568, ECO:0000269|PubMed:34937700, ECO:0000312|PDB:7N8O

Chain	Residue	Details
V	HIS66
d	TRP266-GLY288
c	GLU256-PHE276
c	TRP412-ARG436
V	HIS117
v	HIS66
v	HIS117
D	TRP266-GLY288
d	TRP32-THR53
d	GLY109-GLU131
d	TYR141-LEU162
d	TRP191-THR217

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: axial binding residue => ECO:0000255|HAMAP-Rule:MF_00643, ECO:0000269|PubMed:34937700, ECO:0000305|PubMed:1904816, ECO:0000312|PDB:7N8O

Chain	Residue	Details
F	HIS23
f	HIS23
D	LEU289-LEU352
d	PHE54-GLY108
d	GLY163-ASN190
d	LEU289-LEU352

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: axial binding residue => ECO:0000255|HAMAP-Rule:MF_01383, ECO:0000269|PubMed:34937700, ECO:0000312|PDB:7N8O

Chain	Residue	Details
D	HIS117
D	HIS197
d	HIS117
d	HIS197

---

site_id	SWS_FT_FI5
Number of Residues	10
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01383, ECO:0000269|PubMed:34937700, ECO:0000312|PDB:7N8O

Chain	Residue	Details
D	GLN129
d	HIS268
a	GLU170
a	GLU189
a	HIS215
a	SER264
a	HIS272
a	HIS332
a	HIS337
a	ASP342
D	ASN142
D	HIS214
D	PHE261
D	HIS268
d	GLN129
d	ASN142
d	HIS214
d	PHE261

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:34937700, ECO:0000312|PDB:7N8O

Chain	Residue	Details
A	TYR147
A	PHE265
a	TYR147
a	PHE265

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01379

Chain	Residue	Details
A	GLU333
a	GLU333

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01379, ECO:0000305|PubMed:34937700, ECO:0000312|PDB:7N8O

Chain	Residue	Details
A	ALA344
a	ALA344

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	SITE: Tyrosine radical intermediate => ECO:0000255|HAMAP-Rule:MF_01379

Chain	Residue	Details
A	TYR161
a	TYR161

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	SITE: Stabilizes free radical intermediate => ECO:0000255|HAMAP-Rule:MF_01379

Chain	Residue	Details
A	HIS190
a	HIS190

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	SITE: Cleavage; by CtpA => ECO:0000255|HAMAP-Rule:MF_01379, ECO:0000269|PubMed:34937700, ECO:0000305|PubMed:8034700, ECO:0000312|PDB:7N8O

Chain	Residue	Details
A	ALA344
a	ALA344

---