8THL
Cryo-EM structure of epinephrine-bound alpha-1A-adrenergic receptor in complex with heterotrimeric Gq-protein
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003924 | molecular_function | GTPase activity |
| A | 0005525 | molecular_function | GTP binding |
| A | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| A | 0019001 | molecular_function | guanyl nucleotide binding |
| A | 0031683 | molecular_function | G-protein beta/gamma-subunit complex binding |
| B | 0001750 | cellular_component | photoreceptor outer segment |
| B | 0003924 | molecular_function | GTPase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005765 | cellular_component | lysosomal membrane |
| B | 0005829 | cellular_component | cytosol |
| B | 0005834 | cellular_component | heterotrimeric G-protein complex |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0007165 | biological_process | signal transduction |
| B | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| B | 0007191 | biological_process | adenylate cyclase-activating dopamine receptor signaling pathway |
| B | 0007200 | biological_process | phospholipase C-activating G protein-coupled receptor signaling pathway |
| B | 0007213 | biological_process | G protein-coupled acetylcholine receptor signaling pathway |
| B | 0007265 | biological_process | Ras protein signal transduction |
| B | 0008283 | biological_process | cell population proliferation |
| B | 0016020 | cellular_component | membrane |
| B | 0030159 | molecular_function | signaling receptor complex adaptor activity |
| B | 0044877 | molecular_function | protein-containing complex binding |
| B | 0045202 | cellular_component | synapse |
| B | 0050909 | biological_process | sensory perception of taste |
| B | 0051020 | molecular_function | GTPase binding |
| B | 0060041 | biological_process | retina development in camera-type eye |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 0071380 | biological_process | cellular response to prostaglandin E stimulus |
| B | 0071870 | biological_process | cellular response to catecholamine stimulus |
| B | 0097381 | cellular_component | photoreceptor disc membrane |
| B | 1903561 | cellular_component | extracellular vesicle |
| G | 0005515 | molecular_function | protein binding |
| G | 0005834 | cellular_component | heterotrimeric G-protein complex |
| G | 0005886 | cellular_component | plasma membrane |
| G | 0007165 | biological_process | signal transduction |
| G | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| G | 0007191 | biological_process | adenylate cyclase-activating dopamine receptor signaling pathway |
| G | 0016020 | cellular_component | membrane |
| G | 0031681 | molecular_function | G-protein beta-subunit binding |
| G | 0048144 | biological_process | fibroblast proliferation |
| G | 0070062 | cellular_component | extracellular exosome |
| G | 0071380 | biological_process | cellular response to prostaglandin E stimulus |
| G | 0071870 | biological_process | cellular response to catecholamine stimulus |
| R | 0003796 | molecular_function | lysozyme activity |
| R | 0004930 | molecular_function | G protein-coupled receptor activity |
| R | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| R | 0009253 | biological_process | peptidoglycan catabolic process |
| R | 0016020 | cellular_component | membrane |
| R | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| R | 0016998 | biological_process | cell wall macromolecule catabolic process |
| R | 0030430 | cellular_component | host cell cytoplasm |
| R | 0031640 | biological_process | killing of cells of another organism |
| R | 0042742 | biological_process | defense response to bacterium |
| R | 0044659 | biological_process | viral release from host cell by cytolysis |
Functional Information from PROSITE/UniProt
| site_id | PS00237 |
| Number of Residues | 17 |
| Details | G_PROTEIN_RECEP_F1_1 G-protein coupled receptors family 1 signature. ASImGLCIISIDRYIgV |
| Chain | Residue | Details |
| R | ALA112-VAL128 |
| site_id | PS00678 |
| Number of Residues | 15 |
| Details | WD_REPEATS_1 Trp-Asp (WD) repeats signature. LVSAsqDgKLIIWDS |
| Chain | Residue | Details |
| B | LEU70-SER84 | |
| B | ILE157-ILE171 | |
| B | LEU285-ALA299 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton donor/acceptor => ECO:0000255|HAMAP-Rule:MF_04110, ECO:0000269|PubMed:3382407, ECO:0000269|PubMed:7831309, ECO:0000269|PubMed:8266098 |
| Chain | Residue | Details |
| R | GLU-139 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton donor/acceptor => ECO:0000255|HAMAP-Rule:MF_04110, ECO:0000269|PubMed:1892846, ECO:0000269|PubMed:3382407, ECO:0000269|PubMed:7831309, ECO:0000269|PubMed:8266098 |
| Chain | Residue | Details |
| R | ASP-130 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:8266098 |
| Chain | Residue | Details |
| R | LEU-118 | |
| R | PHE-46 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000303|PubMed:7831309 |
| Chain | Residue | Details |
| R | SER-33 | |
| R | ASN-18 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 23 |
| Details | TRANSMEM: Helical; Name=1 => ECO:0000250 |
| Chain | Residue | Details |
| R | LEU28-VAL51 | |
| A | ASP85 | |
| A | LEU154 | |
| A | ALA218 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 99 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000250 |
| Chain | Residue | Details |
| R | ALA52-TYR64 | |
| R | ASP123-GLY143 | |
| R | ARG206-PHE301 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 23 |
| Details | TRANSMEM: Helical; Name=2 => ECO:0000250 |
| Chain | Residue | Details |
| R | ILE65-VAL88 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 30 |
| Details | TOPO_DOM: Extracellular => ECO:0000250 |
| Chain | Residue | Details |
| R | LEU89-CYS99 | |
| R | PRO168-GLU181 | |
| R | TYR326-PHE333 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 22 |
| Details | TRANSMEM: Helical; Name=3 => ECO:0000250 |
| Chain | Residue | Details |
| R | ASN100-ILE122 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 23 |
| Details | TRANSMEM: Helical; Name=4 => ECO:0000250 |
| Chain | Residue | Details |
| R | LEU144-GLN167 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 23 |
| Details | TRANSMEM: Helical; Name=5 => ECO:0000250 |
| Chain | Residue | Details |
| R | PRO182-CYS205 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 23 |
| Details | TRANSMEM: Helical; Name=6 => ECO:0000250 |
| Chain | Residue | Details |
| R | LYS302-ILE325 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 23 |
| Details | TRANSMEM: Helical; Name=7 => ECO:0000250 |
| Chain | Residue | Details |
| R | LYS334-PHE357 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine; by PKA => ECO:0000255 |
| Chain | Residue | Details |
| R | SER215 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 1 |
| Details | LIPID: S-palmitoyl cysteine => ECO:0000255 |
| Chain | Residue | Details |
| site_id | SWS_FT_FI16 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255 |
| Chain | Residue | Details |
| R | ASN22 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 2 |
| Details | M-CSA 921 |
| Chain | Residue | Details |
| R | GLU-139 | proton shuttle (general acid/base) |
| R | ASP-130 | covalent catalysis |
