8T08

Preholo-Proteasome from Pre1-1 Pre4-1 Double Mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
a	0005515	molecular_function	protein binding
a	0005634	cellular_component	nucleus
a	0005737	cellular_component	cytoplasm
a	0005829	cellular_component	cytosol
a	0005839	cellular_component	proteasome core complex
a	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
a	0019774	cellular_component	proteasome core complex, beta-subunit complex
a	0030163	biological_process	protein catabolic process
a	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
a	0061133	molecular_function	endopeptidase activator activity
A	0003674	molecular_function	molecular_function
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005839	cellular_component	proteasome core complex
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
A	0019773	cellular_component	proteasome core complex, alpha-subunit complex
A	0030163	biological_process	protein catabolic process
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
b	0005515	molecular_function	protein binding
b	0005634	cellular_component	nucleus
b	0005737	cellular_component	cytoplasm
b	0005789	cellular_component	endoplasmic reticulum membrane
b	0005829	cellular_component	cytosol
b	0005839	cellular_component	proteasome core complex
b	0010498	biological_process	proteasomal protein catabolic process
b	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
b	0019774	cellular_component	proteasome core complex, beta-subunit complex
b	0030163	biological_process	protein catabolic process
b	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
b	0061133	molecular_function	endopeptidase activator activity
B	0003674	molecular_function	molecular_function
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005839	cellular_component	proteasome core complex
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
B	0019773	cellular_component	proteasome core complex, alpha-subunit complex
B	0030163	biological_process	protein catabolic process
B	0034515	cellular_component	proteasome storage granule
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
c	0004175	molecular_function	endopeptidase activity
c	0004298	molecular_function	threonine-type endopeptidase activity
c	0005515	molecular_function	protein binding
c	0005634	cellular_component	nucleus
c	0005737	cellular_component	cytoplasm
c	0005777	cellular_component	peroxisome
c	0005829	cellular_component	cytosol
c	0005839	cellular_component	proteasome core complex
c	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
c	0019774	cellular_component	proteasome core complex, beta-subunit complex
c	0030163	biological_process	protein catabolic process
c	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
c	0080129	biological_process	proteasome core complex assembly
C	0003674	molecular_function	molecular_function
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005839	cellular_component	proteasome core complex
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
C	0019773	cellular_component	proteasome core complex, alpha-subunit complex
C	0030163	biological_process	protein catabolic process
C	0034515	cellular_component	proteasome storage granule
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0080129	biological_process	proteasome core complex assembly
d	0003674	molecular_function	molecular_function
d	0005515	molecular_function	protein binding
d	0005634	cellular_component	nucleus
d	0005737	cellular_component	cytoplasm
d	0005829	cellular_component	cytosol
d	0005839	cellular_component	proteasome core complex
d	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
d	0019774	cellular_component	proteasome core complex, beta-subunit complex
d	0030163	biological_process	protein catabolic process
d	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0003674	molecular_function	molecular_function
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005839	cellular_component	proteasome core complex
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
D	0019773	cellular_component	proteasome core complex, alpha-subunit complex
D	0030163	biological_process	protein catabolic process
D	0034515	cellular_component	proteasome storage granule
D	0042175	cellular_component	nuclear outer membrane-endoplasmic reticulum membrane network
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
e	0004175	molecular_function	endopeptidase activity
e	0004298	molecular_function	threonine-type endopeptidase activity
e	0005515	molecular_function	protein binding
e	0005634	cellular_component	nucleus
e	0005737	cellular_component	cytoplasm
e	0005829	cellular_component	cytosol
e	0005839	cellular_component	proteasome core complex
e	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
e	0019774	cellular_component	proteasome core complex, beta-subunit complex
e	0030163	biological_process	protein catabolic process
e	0034515	cellular_component	proteasome storage granule
e	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0003674	molecular_function	molecular_function
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005839	cellular_component	proteasome core complex
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
E	0019773	cellular_component	proteasome core complex, alpha-subunit complex
E	0030163	biological_process	protein catabolic process
E	0034515	cellular_component	proteasome storage granule
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
f	0003674	molecular_function	molecular_function
f	0005515	molecular_function	protein binding
f	0005737	cellular_component	cytoplasm
f	0043248	biological_process	proteasome assembly
f	0080129	biological_process	proteasome core complex assembly
F	0003674	molecular_function	molecular_function
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005839	cellular_component	proteasome core complex
F	0006511	biological_process	ubiquitin-dependent protein catabolic process
F	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
F	0019773	cellular_component	proteasome core complex, alpha-subunit complex
F	0030163	biological_process	protein catabolic process
F	0034515	cellular_component	proteasome storage granule
F	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
g	0003674	molecular_function	molecular_function
g	0005515	molecular_function	protein binding
g	0005634	cellular_component	nucleus
g	0005737	cellular_component	cytoplasm
g	0005829	cellular_component	cytosol
g	0043248	biological_process	proteasome assembly
g	0060090	molecular_function	molecular adaptor activity
g	0080129	biological_process	proteasome core complex assembly
G	0003729	molecular_function	mRNA binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005839	cellular_component	proteasome core complex
G	0006511	biological_process	ubiquitin-dependent protein catabolic process
G	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
G	0019773	cellular_component	proteasome core complex, alpha-subunit complex
G	0030163	biological_process	protein catabolic process
G	0034515	cellular_component	proteasome storage granule
G	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
h	0003674	molecular_function	molecular_function
h	0005515	molecular_function	protein binding
h	0005634	cellular_component	nucleus
h	0005737	cellular_component	cytoplasm
h	0005829	cellular_component	cytosol
h	0005839	cellular_component	proteasome core complex
h	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
h	0019774	cellular_component	proteasome core complex, beta-subunit complex
h	0030163	biological_process	protein catabolic process
h	0034515	cellular_component	proteasome storage granule
h	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
h	0043248	biological_process	proteasome assembly
H	0003674	molecular_function	molecular_function
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0006511	biological_process	ubiquitin-dependent protein catabolic process
H	0006974	biological_process	DNA damage response
H	0043248	biological_process	proteasome assembly
H	0090363	biological_process	obsolete regulation of proteasome core complex assembly
I	0004175	molecular_function	endopeptidase activity
I	0004298	molecular_function	threonine-type endopeptidase activity
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0005839	cellular_component	proteasome core complex
I	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
I	0019774	cellular_component	proteasome core complex, beta-subunit complex
I	0030163	biological_process	protein catabolic process
I	0034515	cellular_component	proteasome storage granule
I	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0005839	cellular_component	proteasome core complex
J	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
J	0019774	cellular_component	proteasome core complex, beta-subunit complex
J	0030163	biological_process	protein catabolic process
J	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
J	0061133	molecular_function	endopeptidase activator activity
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005737	cellular_component	cytoplasm
K	0005789	cellular_component	endoplasmic reticulum membrane
K	0005829	cellular_component	cytosol
K	0005839	cellular_component	proteasome core complex
K	0010498	biological_process	proteasomal protein catabolic process
K	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
K	0019774	cellular_component	proteasome core complex, beta-subunit complex
K	0030163	biological_process	protein catabolic process
K	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
K	0061133	molecular_function	endopeptidase activator activity
L	0004175	molecular_function	endopeptidase activity
L	0004298	molecular_function	threonine-type endopeptidase activity
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005737	cellular_component	cytoplasm
L	0005777	cellular_component	peroxisome
L	0005829	cellular_component	cytosol
L	0005839	cellular_component	proteasome core complex
L	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
L	0019774	cellular_component	proteasome core complex, beta-subunit complex
L	0030163	biological_process	protein catabolic process
L	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
L	0080129	biological_process	proteasome core complex assembly
M	0003674	molecular_function	molecular_function
M	0005515	molecular_function	protein binding
M	0005634	cellular_component	nucleus
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0005839	cellular_component	proteasome core complex
M	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
M	0019774	cellular_component	proteasome core complex, beta-subunit complex
M	0030163	biological_process	protein catabolic process
M	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
N	0004175	molecular_function	endopeptidase activity
N	0004298	molecular_function	threonine-type endopeptidase activity
N	0005515	molecular_function	protein binding
N	0005634	cellular_component	nucleus
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0005839	cellular_component	proteasome core complex
N	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
N	0019774	cellular_component	proteasome core complex, beta-subunit complex
N	0030163	biological_process	protein catabolic process
N	0034515	cellular_component	proteasome storage granule
N	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
O	0003674	molecular_function	molecular_function
O	0005515	molecular_function	protein binding
O	0005737	cellular_component	cytoplasm
O	0043248	biological_process	proteasome assembly
O	0080129	biological_process	proteasome core complex assembly
P	0003674	molecular_function	molecular_function
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0043248	biological_process	proteasome assembly
P	0060090	molecular_function	molecular adaptor activity
P	0080129	biological_process	proteasome core complex assembly
Q	0003674	molecular_function	molecular_function
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0005839	cellular_component	proteasome core complex
Q	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
Q	0019774	cellular_component	proteasome core complex, beta-subunit complex
Q	0030163	biological_process	protein catabolic process
Q	0034515	cellular_component	proteasome storage granule
Q	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Q	0043248	biological_process	proteasome assembly
R	0003674	molecular_function	molecular_function
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005737	cellular_component	cytoplasm
R	0005739	cellular_component	mitochondrion
R	0005839	cellular_component	proteasome core complex
R	0006511	biological_process	ubiquitin-dependent protein catabolic process
R	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
R	0019773	cellular_component	proteasome core complex, alpha-subunit complex
R	0030163	biological_process	protein catabolic process
R	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
S	0003674	molecular_function	molecular_function
S	0005515	molecular_function	protein binding
S	0005634	cellular_component	nucleus
S	0005737	cellular_component	cytoplasm
S	0005839	cellular_component	proteasome core complex
S	0006511	biological_process	ubiquitin-dependent protein catabolic process
S	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
S	0019773	cellular_component	proteasome core complex, alpha-subunit complex
S	0030163	biological_process	protein catabolic process
S	0034515	cellular_component	proteasome storage granule
S	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
T	0003674	molecular_function	molecular_function
T	0005515	molecular_function	protein binding
T	0005634	cellular_component	nucleus
T	0005737	cellular_component	cytoplasm
T	0005829	cellular_component	cytosol
T	0005839	cellular_component	proteasome core complex
T	0006511	biological_process	ubiquitin-dependent protein catabolic process
T	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
T	0019773	cellular_component	proteasome core complex, alpha-subunit complex
T	0030163	biological_process	protein catabolic process
T	0034515	cellular_component	proteasome storage granule
T	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
T	0080129	biological_process	proteasome core complex assembly
U	0003674	molecular_function	molecular_function
U	0005515	molecular_function	protein binding
U	0005634	cellular_component	nucleus
U	0005737	cellular_component	cytoplasm
U	0005739	cellular_component	mitochondrion
U	0005839	cellular_component	proteasome core complex
U	0006511	biological_process	ubiquitin-dependent protein catabolic process
U	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
U	0019773	cellular_component	proteasome core complex, alpha-subunit complex
U	0030163	biological_process	protein catabolic process
U	0034515	cellular_component	proteasome storage granule
U	0042175	cellular_component	nuclear outer membrane-endoplasmic reticulum membrane network
U	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
V	0003674	molecular_function	molecular_function
V	0005515	molecular_function	protein binding
V	0005634	cellular_component	nucleus
V	0005737	cellular_component	cytoplasm
V	0005839	cellular_component	proteasome core complex
V	0006511	biological_process	ubiquitin-dependent protein catabolic process
V	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
V	0019773	cellular_component	proteasome core complex, alpha-subunit complex
V	0030163	biological_process	protein catabolic process
V	0034515	cellular_component	proteasome storage granule
V	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
W	0003674	molecular_function	molecular_function
W	0005515	molecular_function	protein binding
W	0005634	cellular_component	nucleus
W	0005737	cellular_component	cytoplasm
W	0005839	cellular_component	proteasome core complex
W	0006511	biological_process	ubiquitin-dependent protein catabolic process
W	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
W	0019773	cellular_component	proteasome core complex, alpha-subunit complex
W	0030163	biological_process	protein catabolic process
W	0034515	cellular_component	proteasome storage granule
W	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
X	0003729	molecular_function	mRNA binding
X	0005515	molecular_function	protein binding
X	0005634	cellular_component	nucleus
X	0005737	cellular_component	cytoplasm
X	0005839	cellular_component	proteasome core complex
X	0006511	biological_process	ubiquitin-dependent protein catabolic process
X	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
X	0019773	cellular_component	proteasome core complex, alpha-subunit complex
X	0030163	biological_process	protein catabolic process
X	0034515	cellular_component	proteasome storage granule
X	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Y	0003674	molecular_function	molecular_function
Y	0005515	molecular_function	protein binding
Y	0005634	cellular_component	nucleus
Y	0005737	cellular_component	cytoplasm
Y	0006511	biological_process	ubiquitin-dependent protein catabolic process
Y	0006974	biological_process	DNA damage response
Y	0043248	biological_process	proteasome assembly
Y	0090363	biological_process	obsolete regulation of proteasome core complex assembly
Z	0004175	molecular_function	endopeptidase activity
Z	0004298	molecular_function	threonine-type endopeptidase activity
Z	0005515	molecular_function	protein binding
Z	0005634	cellular_component	nucleus
Z	0005737	cellular_component	cytoplasm
Z	0005829	cellular_component	cytosol
Z	0005839	cellular_component	proteasome core complex
Z	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
Z	0019774	cellular_component	proteasome core complex, beta-subunit complex
Z	0030163	biological_process	protein catabolic process
Z	0034515	cellular_component	proteasome storage granule
Z	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process

Functional Information from PROSITE/UniProt

site_id	PS00388
Number of Residues	23
Details	PROTEASOME_ALPHA_1 Proteasome alpha-type subunits signature. YdlsnSvFSPdGRnfQVEYAvkA

Chain	Residue	Details
G	TYR8-ALA30
C	TYR6-SER28
B	TYR5-ALA27
D	TYR4-ALA26
A	TYR12-ALA34
E	TYR8-ALA30
F	TYR6-ALA28

---

site_id	PS00854
Number of Residues	48
Details	PROTEASOME_BETA_1 Proteasome beta-type subunits signature. MAVtFkdGVILGADsrtttgayianrvtd.KltrvhdkiwccrsGsaAD

Chain	Residue	Details
e	MET23-ASP70
h	ILE45-ASP92
C	ILE36-ASP83
I	VAL33-ASP80
d	LEU32-ASP79
a	VAL13-ASP59
D	VAL34-ASP81
b	LEU5-ASP52
c	LEU79-ASP126

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18407956","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	24
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"22106047","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"17287358","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"17330950","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"17330950","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"17330950","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19779198","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Modified residue: {"description":"N-acetylthreonine","evidences":[{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"9087403","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	8
Details	M-CSA 177

Chain	Residue	Details
I	THR30	covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
I	ASP46	activator, steric locator
I	ARG48	activator
I	LYS62	activator, electrostatic stabiliser
I	GLY76	electrostatic stabiliser
I	SER158	activator, electrostatic stabiliser
I	ASP195	activator, steric locator
I	SER198	activator, electrostatic stabiliser

---

site_id	MCSA2
Number of Residues	8
Details	M-CSA 177

Chain	Residue	Details
Z	THR30	covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
Z	ASP46	activator, steric locator
Z	ARG48	activator
Z	LYS62	activator, electrostatic stabiliser
Z	GLY76	electrostatic stabiliser
Z	SER158	activator, electrostatic stabiliser
Z	ASP195	activator, steric locator
Z	SER198	activator, electrostatic stabiliser

---