8SXN
Structure of NLRP3 and NEK7 complex
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0000922 | cellular_component | spindle pole |
| A | 0004672 | molecular_function | protein kinase activity |
| A | 0004674 | molecular_function | protein serine/threonine kinase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005813 | cellular_component | centrosome |
| A | 0005815 | cellular_component | microtubule organizing center |
| A | 0005856 | cellular_component | cytoskeleton |
| A | 0005874 | cellular_component | microtubule |
| A | 0006468 | biological_process | protein phosphorylation |
| A | 0007346 | biological_process | regulation of mitotic cell cycle |
| A | 0009306 | biological_process | protein secretion |
| A | 0016301 | molecular_function | kinase activity |
| A | 0016740 | molecular_function | transferase activity |
| A | 0032206 | biological_process | positive regulation of telomere maintenance |
| A | 0032731 | biological_process | positive regulation of interleukin-1 beta production |
| A | 0032741 | biological_process | positive regulation of interleukin-18 production |
| A | 0033044 | biological_process | regulation of chromosome organization |
| A | 0035655 | biological_process | interleukin-18-mediated signaling pathway |
| A | 0035865 | biological_process | cellular response to potassium ion |
| A | 0044546 | biological_process | NLRP3 inflammasome complex assembly |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0050729 | biological_process | positive regulation of inflammatory response |
| A | 0051225 | biological_process | spindle assembly |
| A | 0051604 | biological_process | protein maturation |
| A | 0070269 | biological_process | pyroptotic inflammatory response |
| A | 0070498 | biological_process | interleukin-1-mediated signaling pathway |
| A | 0106310 | molecular_function | protein serine kinase activity |
| A | 0140677 | molecular_function | molecular function activator activity |
| A | 1900227 | biological_process | positive regulation of NLRP3 inflammasome complex assembly |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0000922 | cellular_component | spindle pole |
| B | 0004672 | molecular_function | protein kinase activity |
| B | 0004674 | molecular_function | protein serine/threonine kinase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005654 | cellular_component | nucleoplasm |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005813 | cellular_component | centrosome |
| B | 0005815 | cellular_component | microtubule organizing center |
| B | 0005856 | cellular_component | cytoskeleton |
| B | 0005874 | cellular_component | microtubule |
| B | 0006468 | biological_process | protein phosphorylation |
| B | 0007346 | biological_process | regulation of mitotic cell cycle |
| B | 0009306 | biological_process | protein secretion |
| B | 0016301 | molecular_function | kinase activity |
| B | 0016740 | molecular_function | transferase activity |
| B | 0032206 | biological_process | positive regulation of telomere maintenance |
| B | 0032731 | biological_process | positive regulation of interleukin-1 beta production |
| B | 0032741 | biological_process | positive regulation of interleukin-18 production |
| B | 0033044 | biological_process | regulation of chromosome organization |
| B | 0035655 | biological_process | interleukin-18-mediated signaling pathway |
| B | 0035865 | biological_process | cellular response to potassium ion |
| B | 0044546 | biological_process | NLRP3 inflammasome complex assembly |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0050729 | biological_process | positive regulation of inflammatory response |
| B | 0051225 | biological_process | spindle assembly |
| B | 0051604 | biological_process | protein maturation |
| B | 0070269 | biological_process | pyroptotic inflammatory response |
| B | 0070498 | biological_process | interleukin-1-mediated signaling pathway |
| B | 0106310 | molecular_function | protein serine kinase activity |
| B | 0140677 | molecular_function | molecular function activator activity |
| B | 1900227 | biological_process | positive regulation of NLRP3 inflammasome complex assembly |
Functional Information from PROSITE/UniProt
| site_id | PS00108 |
| Number of Residues | 13 |
| Details | PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VmHrDIKpaNVFI |
| Chain | Residue | Details |
| A | VAL157-ILE169 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:35254907, ECO:0000305|PubMed:36142182, ECO:0000305|PubMed:36442502, ECO:0007744|PDB:7VTP, ECO:0007744|PDB:7ZGU, ECO:0007744|PDB:8EJ4 |
| Chain | Residue | Details |
| C | THR169 | |
| D | THR169 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00136, ECO:0000269|PubMed:17483456, ECO:0000305|PubMed:34687713, ECO:0000305|PubMed:35114687, ECO:0000305|PubMed:35254907, ECO:0000305|PubMed:36142182, ECO:0000305|PubMed:36442502, ECO:0007744|PDB:7ALV, ECO:0007744|PDB:7PZC, ECO:0007744|PDB:7VTP, ECO:0007744|PDB:7ZGU, ECO:0007744|PDB:8EJ4 |
| Chain | Residue | Details |
| C | GLY226 | |
| D | GLY226 | |
| B | ILE40 | |
| B | MET63 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:34687713, ECO:0000305|PubMed:35114687, ECO:0000305|PubMed:35254907, ECO:0000305|PubMed:36142182, ECO:0007744|PDB:7ALV, ECO:0007744|PDB:7PZC, ECO:0007744|PDB:7VTP, ECO:0007744|PDB:7ZGU |
| Chain | Residue | Details |
| C | HIS522 | |
| D | HIS522 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | MOD_RES: Phosphotyrosine; by BTK => ECO:0000269|PubMed:34554188 |
| Chain | Residue | Details |
| C | TYR136 | |
| C | TYR140 | |
| C | TYR143 | |
| C | TYR168 | |
| D | TYR136 | |
| D | TYR140 | |
| D | TYR143 | |
| D | TYR168 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q8R4B8 |
| Chain | Residue | Details |
| C | SER161 | |
| C | SER295 | |
| D | SER161 | |
| D | SER295 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 8 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:28943315 |
| Chain | Residue | Details |
| C | SER163 | |
| C | SER334 | |
| C | SER728 | |
| C | SER975 | |
| D | SER163 | |
| D | SER334 | |
| D | SER728 | |
| D | SER975 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine; by MAPK8 => ECO:0000269|PubMed:35114687 |
| Chain | Residue | Details |
| C | SER198 | |
| D | SER198 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:35114687 |
| Chain | Residue | Details |
| C | SER201 | |
| D | SER201 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine; by LATS1 and LATS2 => ECO:0000269|PubMed:39173637 |
| Chain | Residue | Details |
| C | SER265 | |
| D | SER265 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 6 |
| Details | MOD_RES: Phosphoserine; by CSNK1A1 => ECO:0000269|PubMed:34615873 |
| Chain | Residue | Details |
| C | SER735 | |
| C | SER806 | |
| C | SER1035 | |
| D | SER735 | |
| D | SER806 | |
| D | SER1035 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000269|PubMed:27043286 |
| Chain | Residue | Details |
| C | TYR861 | |
| D | TYR861 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 6 |
| Details | LIPID: S-palmitoyl cysteine => ECO:0000269|PubMed:36586411 |
| Chain | Residue | Details |
| C | CYS837 | |
| C | CYS838 | |
| C | CYS844 | |
| D | CYS837 | |
| D | CYS838 | |
| D | CYS844 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 2 |
| Details | LIPID: S-palmitoyl cysteine => ECO:0000269|PubMed:39173637 |
| Chain | Residue | Details |
| C | CYS958 | |
| D | CYS958 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:26037928 |
| Chain | Residue | Details |
| C | LYS689 | |
| D | LYS689 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 8 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:34615873 |
| Chain | Residue | Details |
| C | LYS878 | |
| C | LYS927 | |
| C | LYS973 | |
| D | LYS878 | |
| D | LYS927 | |
| D | LYS973 |
