Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8SXN

Structure of NLRP3 and NEK7 complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000922	cellular_component	spindle pole
A	0004672	molecular_function	protein kinase activity
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005813	cellular_component	centrosome
A	0005815	cellular_component	microtubule organizing center
A	0005874	cellular_component	microtubule
A	0006468	biological_process	protein phosphorylation
A	0007346	biological_process	regulation of mitotic cell cycle
A	0016301	molecular_function	kinase activity
A	0016740	molecular_function	transferase activity
A	0032206	biological_process	positive regulation of telomere maintenance
A	0033044	biological_process	regulation of chromosome organization
A	0035865	biological_process	cellular response to potassium ion
A	0046872	molecular_function	metal ion binding
A	0051225	biological_process	spindle assembly
A	0106310	molecular_function	protein serine kinase activity
A	0140677	molecular_function	molecular function activator activity
A	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
B	0000166	molecular_function	nucleotide binding
B	0000922	cellular_component	spindle pole
B	0004672	molecular_function	protein kinase activity
B	0004674	molecular_function	protein serine/threonine kinase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005813	cellular_component	centrosome
B	0005815	cellular_component	microtubule organizing center
B	0005874	cellular_component	microtubule
B	0006468	biological_process	protein phosphorylation
B	0007346	biological_process	regulation of mitotic cell cycle
B	0016301	molecular_function	kinase activity
B	0016740	molecular_function	transferase activity
B	0032206	biological_process	positive regulation of telomere maintenance
B	0033044	biological_process	regulation of chromosome organization
B	0035865	biological_process	cellular response to potassium ion
B	0046872	molecular_function	metal ion binding
B	0051225	biological_process	spindle assembly
B	0106310	molecular_function	protein serine kinase activity
B	0140677	molecular_function	molecular function activator activity
B	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly

Functional Information from PROSITE/UniProt

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VmHrDIKpaNVFI

Chain	Residue	Details
A	VAL157-ILE169

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	530
Details	Domain: {"description":"Protein kinase","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	26
Details	Region: {"description":"NTE motif","evidences":[{"source":"PubMed","id":"19941817","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	2
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10027","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	18
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19941817","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"2WQN","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	2
Details	Site: {"description":"Autoinhibitory","evidences":[{"source":"PubMed","id":"19941817","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine; by NEK9","evidences":[{"source":"PubMed","id":"12840024","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"26522158","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	40
Details	Repeat: {"description":"LRR 1"}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	42
Details	Repeat: {"description":"LRR 2"}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	40
Details	Repeat: {"description":"LRR 3"}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	42
Details	Repeat: {"description":"LRR 4"}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	40
Details	Repeat: {"description":"LRR 5"}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	42
Details	Repeat: {"description":"LRR 6"}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	40
Details	Repeat: {"description":"LRR 7"}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	42
Details	Repeat: {"description":"LRR 8"}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	42
Details	Repeat: {"description":"LRR 9"}

Chain

Residue

Details

site_id	SWS_FT_FI16
Number of Residues	8
Details	Motif: {"description":"KFERQ-like motif 1","evidences":[{"source":"PubMed","id":"36586411","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI17
Number of Residues	8
Details	Motif: {"description":"KFERQ-like motif 2","evidences":[{"source":"PubMed","id":"36586411","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI18
Number of Residues	8
Details	Motif: {"description":"KFERQ-like motif 3","evidences":[{"source":"PubMed","id":"36586411","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI19
Number of Residues	8
Details	Motif: {"description":"KFERQ-like motif 4","evidences":[{"source":"PubMed","id":"36586411","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI20
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"35254907","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"36142182","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"36442502","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"7VTP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7ZGU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8EJ4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI21
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00136","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17483456","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34687713","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"35114687","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"35254907","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"36142182","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"36442502","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"7ALV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7PZC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7VTP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7ZGU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8EJ4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI22
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"34687713","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"35114687","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"35254907","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"36142182","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"7ALV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7PZC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7VTP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7ZGU","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI23
Number of Residues	8
Details	Modified residue: {"description":"Phosphotyrosine; by BTK","evidences":[{"source":"PubMed","id":"34554188","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI24
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"Q8R4B8","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI25
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"28943315","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI26
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"35114687","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI27
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine; by LATS1 and LATS2","evidences":[{"source":"PubMed","id":"39173637","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI28
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine; by CSNK1A1","evidences":[{"source":"PubMed","id":"34615873","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI29
Number of Residues	2
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"27043286","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI30
Number of Residues	6
Details	Lipidation: {"description":"S-palmitoyl cysteine","evidences":[{"source":"PubMed","id":"36586411","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI31
Number of Residues	2
Details	Lipidation: {"description":"S-palmitoyl cysteine","evidences":[{"source":"PubMed","id":"39173637","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI32
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"37575012","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI33
Number of Residues	8
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"34615873","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)