8SBS

Fumarate C - R126A in (3-(N-morpholino)propanesulfonic acid) at pH 7.5

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004333	molecular_function	fumarate hydratase activity
A	0005737	cellular_component	cytoplasm
A	0006099	biological_process	tricarboxylic acid cycle
A	0006106	biological_process	fumarate metabolic process
A	0006108	biological_process	malate metabolic process
A	0006979	biological_process	response to oxidative stress
A	0016829	molecular_function	lyase activity
A	0042802	molecular_function	identical protein binding

Functional Information from PROSITE/UniProt

site_id	PS00163
Number of Residues	10
Details	FUMARATE_LYASES Fumarate lyases signature. GSsiMpGKvN

Chain	Residue	Details
A	GLY317-ASN326

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_00743","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"9098893","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00743","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12021453","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"8909293","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"9098893","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1FUO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1FUP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1FUQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1KQ7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2FUS","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"8909293","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9098893","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1FUO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1FUP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1FUQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1FUR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI4
Number of Residues	3
Details	Binding site: {"description":"in site B","evidences":[{"source":"PubMed","id":"12021453","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8909293","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9098893","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1FUO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1FUP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1FUQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1FUR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1KQ7","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00743","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12021453","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8909293","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9098893","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1FUO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1FUP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1FUQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1KQ7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2FUS","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00743","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Site: {"description":"Important for catalytic activity","evidences":[{"source":"HAMAP-Rule","id":"MF_00743","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"8909293","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	3
Details	M-CSA 569

Chain	Residue	Details
A	HIS188	electrostatic stabiliser, proton acceptor
A	LYS324	electrostatic stabiliser
A	GLU331	increase basicity

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