8RHJ
Yeast 20S proteasome in complex with a macrocyclic oxindole epoxyketone (compound 5)
This is a non-PDB format compatible entry.
Functional Information from PROSITE/UniProt
| site_id | PS00388 |
| Number of Residues | 23 |
| Details | PROTEASOME_ALPHA_1 Proteasome alpha-type subunits signature. YdralSiFSPdGHifQVEYAleA |
| Chain | Residue | Details |
| C | TYR2-ALA24 | |
| G | TYR3-ALA25 | |
| A | TYR5-ALA27 | |
| D | TYR0-ALA22 | |
| F | TYR4-ALA26 | |
| E | TYR5-ALA27 | |
| B | TYR5-SER27 |
| site_id | PS00854 |
| Number of Residues | 48 |
| Details | PROTEASOME_BETA_1 Proteasome beta-type subunits signature. LGIaGedFAVLAGDtrnitdysinsryep.KvfdcgdnivmsanGfaAD |
| Chain | Residue | Details |
| K | LEU9-ASP56 | |
| b | MET9-ASP56 | |
| L | LEU13-ASP60 | |
| C | VAL32-ASP79 | |
| I | VAL12-ASP58 | |
| J | LEU5-ASP52 | |
| a | ILE12-ASP59 | |
| H | VAL9-ASP56 | |
| B | ILE35-ASP82 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Nucleophile => ECO:0000269|PubMed:9087403 |
| Chain | Residue | Details |
| b | THR6 | |
| B | LYS198 | |
| B | LYS230 | |
| P | LYS99 | |
| P | LYS198 | |
| P | LYS230 | |
| N | THR6 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: |
| Chain | Residue | Details |
| Y | ALA54 | |
| W | LYS69 | |
| K | ALA54 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047 |
| Chain | Residue | Details |
| G | LYS223 | |
| R | SER243 | |
| U | LYS223 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 8 |
| Details | M-CSA 177 |
| Chain | Residue | Details |
| H | THR6 | covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor |
| H | ASP22 | activator, steric locator |
| H | ARG24 | activator |
| H | LYS38 | activator, electrostatic stabiliser |
| H | GLY52 | electrostatic stabiliser |
| H | SER134 | activator, electrostatic stabiliser |
| H | ASP171 | activator, steric locator |
| H | SER174 | activator, electrostatic stabiliser |
| site_id | MCSA2 |
| Number of Residues | 8 |
| Details | M-CSA 177 |
| Chain | Residue | Details |
| V | THR6 | covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor |
| V | ASP22 | activator, steric locator |
| V | ARG24 | activator |
| V | LYS38 | activator, electrostatic stabiliser |
| V | GLY52 | electrostatic stabiliser |
| V | SER134 | activator, electrostatic stabiliser |
| V | ASP171 | activator, steric locator |
| V | SER174 | activator, electrostatic stabiliser |
