8R85
Xylanase from Bacillus circulans mutant E78Q/W9A
Functional Information from PROSITE/UniProt
| site_id | PS00777 |
| Number of Residues | 12 |
| Details | GH11_2 Glycosyl hydrolases family 11 (GH11) active site signature 2. MatEGYQSSGsS |
| Chain | Residue | Details |
| A | MET169-SER180 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Nucleophile => ECO:0000255|PROSITE-ProRule:PRU10062, ECO:0000269|PubMed:8019418 |
| Chain | Residue | Details |
| A | GLN78 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton donor => ECO:0000255|PROSITE-ProRule:PRU10063, ECO:0000269|PubMed:8019418 |
| Chain | Residue | Details |
| A | GLU172 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 432 |
| Chain | Residue | Details |
| A | ASN35 | modifies pKa |
| A | TYR69 | electrostatic destabiliser |
| A | GLN78 | covalent catalysis, proton shuttle (general acid/base) |
| A | TYR80 | modifies pKa |
| A | GLU172 | proton shuttle (general acid/base) |
