8R3M

Mycobacterium smegnatis RNA polymerase transcription initiation complex with SigmaA, RbpA, HelD N-terminal, CO and PCh loop domain, and an upstream-fork promoter fragment; State III conformation

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003899	molecular_function	DNA-directed RNA polymerase activity
A	0005737	cellular_component	cytoplasm
A	0006351	biological_process	DNA-templated transcription
A	0034062	molecular_function	5'-3' RNA polymerase activity
A	0046983	molecular_function	protein dimerization activity
B	0003677	molecular_function	DNA binding
B	0003899	molecular_function	DNA-directed RNA polymerase activity
B	0005737	cellular_component	cytoplasm
B	0006351	biological_process	DNA-templated transcription
B	0034062	molecular_function	5'-3' RNA polymerase activity
B	0046983	molecular_function	protein dimerization activity
C	0003677	molecular_function	DNA binding
C	0003899	molecular_function	DNA-directed RNA polymerase activity
C	0005515	molecular_function	protein binding
C	0006351	biological_process	DNA-templated transcription
C	0032549	molecular_function	ribonucleoside binding
C	0034062	molecular_function	5'-3' RNA polymerase activity
D	0000287	molecular_function	magnesium ion binding
D	0003677	molecular_function	DNA binding
D	0003899	molecular_function	DNA-directed RNA polymerase activity
D	0006351	biological_process	DNA-templated transcription
D	0008270	molecular_function	zinc ion binding
D	0034062	molecular_function	5'-3' RNA polymerase activity
E	0003677	molecular_function	DNA binding
E	0003899	molecular_function	DNA-directed RNA polymerase activity
E	0006351	biological_process	DNA-templated transcription
E	0034062	molecular_function	5'-3' RNA polymerase activity
F	0003677	molecular_function	DNA binding
F	0003700	molecular_function	DNA-binding transcription factor activity
F	0005737	cellular_component	cytoplasm
F	0006352	biological_process	DNA-templated transcription initiation
F	0006355	biological_process	regulation of DNA-templated transcription
F	0010468	biological_process	regulation of gene expression
F	0016987	molecular_function	sigma factor activity
F	2000142	biological_process	regulation of DNA-templated transcription initiation
H	0000725	biological_process	recombinational repair
H	0003677	molecular_function	DNA binding
H	0003678	molecular_function	DNA helicase activity
H	0003711	molecular_function	transcription elongation factor activity
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0043138	molecular_function	3'-5' DNA helicase activity
J	0001000	molecular_function	bacterial-type RNA polymerase core enzyme binding
J	0045893	biological_process	positive regulation of DNA-templated transcription
J	0046677	biological_process	response to antibiotic

Functional Information from PROSITE/UniProt

site_id	PS00715
Number of Residues	14
Details	SIGMA70_1 Sigma-70 factors family signature 1. DLIQeGnLGLIrAV

Chain	Residue	Details
F	ASP257-VAL270

---

site_id	PS00716
Number of Residues	27
Details	SIGMA70_2 Sigma-70 factors family signature 2. TldEIGqvygVTrerIrQIEsktMskL

Chain	Residue	Details
F	THR426-LEU452

---

site_id	PS00867
Number of Residues	8
Details	CPSASE_2 Carbamoyl-phosphate synthase subdomain signature 2. LLEANLRL

Chain	Residue	Details
F	LEU233-LEU240

---

site_id	PS01166
Number of Residues	13
Details	RNA_POL_BETA RNA polymerases beta chain signature. GdKLAGrHGNKGV

Chain	Residue	Details
C	GLY873-VAL885

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	450
Details	Region: {"description":"Alpha N-terminal domain (alpha-NTD)","evidences":[{"source":"HAMAP-Rule","id":"MF_00059","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	11
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01322","evidenceCode":"ECO:0000255"},{"source":"PDB","id":"6YXU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6YYS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6Z11","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	19
Details	DNA binding: {"description":"H-T-H motif","evidences":[{"source":"HAMAP-Rule","id":"MF_00963","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	70
Details	Region: {"description":"Sigma-70 factor domain-2","evidences":[{"source":"HAMAP-Rule","id":"MF_00963","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	76
Details	Region: {"description":"Sigma-70 factor domain-3","evidences":[{"source":"HAMAP-Rule","id":"MF_00963","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	53
Details	Region: {"description":"Sigma-70 factor domain-4","evidences":[{"source":"HAMAP-Rule","id":"MF_00963","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	3
Details	Motif: {"description":"Interaction with polymerase core subunit RpoC","evidences":[{"source":"HAMAP-Rule","id":"MF_00963","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	56
Details	Coiled coil: {"evidences":[{"source":"PubMed","id":"33339823","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6YXU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6YYS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6Z11","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"33339823","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6YYS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6Z11","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---