8QQU
Asymetric subunit of E. coli DNA gyrase bound to a linear part of a DNA minicircle
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| C | 0003677 | molecular_function | DNA binding |
| C | 0003916 | molecular_function | DNA topoisomerase activity |
| C | 0003918 | molecular_function | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005524 | molecular_function | ATP binding |
| C | 0005694 | cellular_component | chromosome |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006259 | biological_process | DNA metabolic process |
| C | 0006261 | biological_process | DNA-templated DNA replication |
| C | 0006265 | biological_process | DNA topological change |
| C | 0006351 | biological_process | DNA-templated transcription |
| C | 0008094 | molecular_function | ATP-dependent activity, acting on DNA |
| C | 0009330 | cellular_component | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex |
| C | 0009410 | biological_process | response to xenobiotic stimulus |
| C | 0016020 | cellular_component | membrane |
| C | 0034335 | molecular_function | DNA negative supercoiling activity |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0046677 | biological_process | response to antibiotic |
| C | 0051276 | biological_process | chromosome organization |
| C | 2000104 | biological_process | negative regulation of DNA-templated DNA replication |
| D | 0003677 | molecular_function | DNA binding |
| D | 0003916 | molecular_function | DNA topoisomerase activity |
| D | 0003918 | molecular_function | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005524 | molecular_function | ATP binding |
| D | 0005694 | cellular_component | chromosome |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006261 | biological_process | DNA-templated DNA replication |
| D | 0006265 | biological_process | DNA topological change |
| D | 0006351 | biological_process | DNA-templated transcription |
| D | 0008094 | molecular_function | ATP-dependent activity, acting on DNA |
| D | 0009330 | cellular_component | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex |
| D | 0009410 | biological_process | response to xenobiotic stimulus |
| D | 0034335 | molecular_function | DNA negative supercoiling activity |
| D | 0046677 | biological_process | response to antibiotic |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0051276 | biological_process | chromosome organization |
Functional Information from PROSITE/UniProt
| site_id | PS00177 |
| Number of Residues | 9 |
| Details | TOPOISOMERASE_II DNA topoisomerase II signature. LVEGDSAGG |
| Chain | Residue | Details |
| D | LEU422-GLY430 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton acceptor (ATPase activity) => ECO:0000305|PubMed:10734094, ECO:0000305|PubMed:8248233 |
| Chain | Residue | Details |
| D | GLU42 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:25202966, ECO:0000269|PubMed:25849408, ECO:0000305|PubMed:10734094 |
| Chain | Residue | Details |
| D | TYR5 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 5 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10734094, ECO:0000269|PubMed:25202966, ECO:0000269|PubMed:25849408 |
| Chain | Residue | Details |
| D | ASN46 | |
| D | ASP73 | |
| D | GLY102 | |
| D | TYR109 | |
| D | LEU115 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 7 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:25849408 |
| Chain | Residue | Details |
| D | ILE94 | |
| D | VAL97 | |
| D | ALA100 | |
| D | LYS103 | |
| D | ASP105 | |
| D | GLY117 | |
| D | SER121 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10734094, ECO:0000269|PubMed:25849408 |
| Chain | Residue | Details |
| D | GLN335 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 3 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01898, ECO:0000305|PubMed:12051843, ECO:0000305|PubMed:18642932 |
| Chain | Residue | Details |
| D | GLU424 | |
| D | ASP498 | |
| D | ASP500 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | SITE: Transition state stabilizer => ECO:0000305|PubMed:9657678 |
| Chain | Residue | Details |
| D | LYS337 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | SITE: Interaction with DNA => ECO:0000255|HAMAP-Rule:MF_01898 |
| Chain | Residue | Details |
| D | LYS449 | |
| D | ASN452 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 3 |
| Details | M-CSA 745 |
| Chain | Residue | Details |
| C | ARG32 | electrostatic stabiliser |
| C | HIS78 | proton acceptor |
| C | PHE122 | metal ligand, nucleofuge, nucleophile, proton acceptor, proton donor |
