8QQU

Asymetric subunit of E. coli DNA gyrase bound to a linear part of a DNA minicircle

Functional Information from GO Data

Chain	GOid	namespace	contents
C	0000166	molecular_function	nucleotide binding
C	0003677	molecular_function	DNA binding
C	0003916	molecular_function	DNA topoisomerase activity
C	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005694	cellular_component	chromosome
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006259	biological_process	DNA metabolic process
C	0006261	biological_process	DNA-templated DNA replication
C	0006265	biological_process	DNA topological change
C	0006351	biological_process	DNA-templated transcription
C	0008094	molecular_function	ATP-dependent activity, acting on DNA
C	0009330	cellular_component	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
C	0009410	biological_process	response to xenobiotic stimulus
C	0016020	cellular_component	membrane
C	0016853	molecular_function	isomerase activity
C	0034335	molecular_function	DNA negative supercoiling activity
C	0042802	molecular_function	identical protein binding
C	0046677	biological_process	response to antibiotic
C	0051276	biological_process	chromosome organization
C	2000104	biological_process	negative regulation of DNA-templated DNA replication
D	0000166	molecular_function	nucleotide binding
D	0003677	molecular_function	DNA binding
D	0003916	molecular_function	DNA topoisomerase activity
D	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005694	cellular_component	chromosome
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006261	biological_process	DNA-templated DNA replication
D	0006265	biological_process	DNA topological change
D	0006351	biological_process	DNA-templated transcription
D	0008094	molecular_function	ATP-dependent activity, acting on DNA
D	0009330	cellular_component	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
D	0009410	biological_process	response to xenobiotic stimulus
D	0016853	molecular_function	isomerase activity
D	0034335	molecular_function	DNA negative supercoiling activity
D	0046677	biological_process	response to antibiotic
D	0046872	molecular_function	metal ion binding
D	0051276	biological_process	chromosome organization

Functional Information from PROSITE/UniProt

site_id	PS00177
Number of Residues	9
Details	TOPOISOMERASE_II DNA topoisomerase II signature. LVEGDSAGG

Chain	Residue	Details
D	LEU422-GLY430

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton acceptor (ATPase activity) => ECO:0000305|PubMed:10734094, ECO:0000305|PubMed:8248233

Chain	Residue	Details
D	GLU42

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site_id	SWS_FT_FI2
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:25202966, ECO:0000269|PubMed:25849408, ECO:0000305|PubMed:10734094

Chain	Residue	Details
D	TYR5

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site_id	SWS_FT_FI3
Number of Residues	5
Details	BINDING: BINDING => ECO:0000269|PubMed:10734094, ECO:0000269|PubMed:25202966, ECO:0000269|PubMed:25849408

Chain	Residue	Details
D	ASN46
D	ASP73
D	GLY102
D	TYR109
D	LEU115

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site_id	SWS_FT_FI4
Number of Residues	7
Details	BINDING: BINDING => ECO:0000269|PubMed:25849408

Chain	Residue	Details
D	ILE94
D	VAL97
D	ALA100
D	LYS103
D	ASP105
D	GLY117
D	SER121

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site_id	SWS_FT_FI5
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:10734094, ECO:0000269|PubMed:25849408

Chain	Residue	Details
D	GLN335

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site_id	SWS_FT_FI6
Number of Residues	3
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01898, ECO:0000305|PubMed:12051843, ECO:0000305|PubMed:18642932

Chain	Residue	Details
D	GLU424
D	ASP498
D	ASP500

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site_id	SWS_FT_FI7
Number of Residues	1
Details	SITE: Transition state stabilizer => ECO:0000305|PubMed:9657678

Chain	Residue	Details
D	LYS337

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site_id	SWS_FT_FI8
Number of Residues	2
Details	SITE: Interaction with DNA => ECO:0000255|HAMAP-Rule:MF_01898

Chain	Residue	Details
D	LYS449
D	ASN452

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	3
Details	M-CSA 745

Chain	Residue	Details
C	ARG32	electrostatic stabiliser
C	HIS78	proton acceptor
C	PHE122	metal ligand, nucleofuge, nucleophile, proton acceptor, proton donor

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