8QQU
Asymetric subunit of E. coli DNA gyrase bound to a linear part of a DNA minicircle
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0003677 | molecular_function | DNA binding |
| C | 0003916 | molecular_function | DNA topoisomerase activity |
| C | 0003918 | molecular_function | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005524 | molecular_function | ATP binding |
| C | 0005694 | cellular_component | chromosome |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006259 | biological_process | DNA metabolic process |
| C | 0006261 | biological_process | DNA-templated DNA replication |
| C | 0006265 | biological_process | DNA topological change |
| C | 0006351 | biological_process | DNA-templated transcription |
| C | 0008094 | molecular_function | ATP-dependent activity, acting on DNA |
| C | 0009330 | cellular_component | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex |
| C | 0009410 | biological_process | response to xenobiotic stimulus |
| C | 0016020 | cellular_component | membrane |
| C | 0016853 | molecular_function | isomerase activity |
| C | 0034335 | molecular_function | DNA negative supercoiling activity |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0046677 | biological_process | response to antibiotic |
| C | 0051276 | biological_process | chromosome organization |
| C | 2000104 | biological_process | negative regulation of DNA-templated DNA replication |
| D | 0000166 | molecular_function | nucleotide binding |
| D | 0003677 | molecular_function | DNA binding |
| D | 0003916 | molecular_function | DNA topoisomerase activity |
| D | 0003918 | molecular_function | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005524 | molecular_function | ATP binding |
| D | 0005694 | cellular_component | chromosome |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006261 | biological_process | DNA-templated DNA replication |
| D | 0006265 | biological_process | DNA topological change |
| D | 0006351 | biological_process | DNA-templated transcription |
| D | 0008094 | molecular_function | ATP-dependent activity, acting on DNA |
| D | 0009330 | cellular_component | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex |
| D | 0009410 | biological_process | response to xenobiotic stimulus |
| D | 0016853 | molecular_function | isomerase activity |
| D | 0034335 | molecular_function | DNA negative supercoiling activity |
| D | 0046677 | biological_process | response to antibiotic |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0051276 | biological_process | chromosome organization |
Functional Information from PROSITE/UniProt
| site_id | PS00177 |
| Number of Residues | 9 |
| Details | TOPOISOMERASE_II DNA topoisomerase II signature. LVEGDSAGG |
| Chain | Residue | Details |
| D | LEU422-GLY430 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | Active site: {"description":"O-(5'-phospho-DNA)-tyrosine intermediate","evidences":[{"source":"HAMAP-Rule","id":"MF_01897","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"3031051","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 115 |
| Details | Domain: {"description":"Toprim","evidences":[{"source":"HAMAP-Rule","id":"MF_01898","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 171 |
| Details | Region: {"description":"Transducer domain","evidences":[{"source":"PubMed","id":"10734094","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"1646964","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Proton acceptor (ATPase activity)","evidences":[{"source":"PubMed","id":"10734094","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"8248233","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"10734094","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25202966","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25849408","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"25849408","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"10734094","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25849408","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 3 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01898","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12051843","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"18642932","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | Site: {"description":"Transition state stabilizer","evidences":[{"source":"PubMed","id":"9657678","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | Site: {"description":"Interaction with DNA","evidences":[{"source":"HAMAP-Rule","id":"MF_01898","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
