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8QA0

Cryo-EM structure of Cx26 solubilised in LMNG - hemichannel classification - NConst conformation

Functional Information from GO Data
ChainGOidnamespacecontents
A0005243molecular_functiongap junction channel activity
A0005509molecular_functioncalcium ion binding
A0005515molecular_functionprotein binding
A0005793cellular_componentendoplasmic reticulum-Golgi intermediate compartment
A0005886cellular_componentplasma membrane
A0005921cellular_componentgap junction
A0005922cellular_componentconnexin complex
A0007154biological_processcell communication
A0007267biological_processcell-cell signaling
A0007605biological_processsensory perception of sound
A0010644biological_processcell communication by electrical coupling
A0016020cellular_componentmembrane
A0016264biological_processgap junction assembly
A0042802molecular_functionidentical protein binding
A0046872molecular_functionmetal ion binding
A0055085biological_processtransmembrane transport
A0070161cellular_componentanchoring junction
A1903763molecular_functiongap junction channel activity involved in cell communication by electrical coupling
A1990349biological_processgap junction-mediated intercellular transport
B0005243molecular_functiongap junction channel activity
B0005509molecular_functioncalcium ion binding
B0005515molecular_functionprotein binding
B0005793cellular_componentendoplasmic reticulum-Golgi intermediate compartment
B0005886cellular_componentplasma membrane
B0005921cellular_componentgap junction
B0005922cellular_componentconnexin complex
B0007154biological_processcell communication
B0007267biological_processcell-cell signaling
B0007605biological_processsensory perception of sound
B0010644biological_processcell communication by electrical coupling
B0016020cellular_componentmembrane
B0016264biological_processgap junction assembly
B0042802molecular_functionidentical protein binding
B0046872molecular_functionmetal ion binding
B0055085biological_processtransmembrane transport
B0070161cellular_componentanchoring junction
B1903763molecular_functiongap junction channel activity involved in cell communication by electrical coupling
B1990349biological_processgap junction-mediated intercellular transport
C0005243molecular_functiongap junction channel activity
C0005509molecular_functioncalcium ion binding
C0005515molecular_functionprotein binding
C0005793cellular_componentendoplasmic reticulum-Golgi intermediate compartment
C0005886cellular_componentplasma membrane
C0005921cellular_componentgap junction
C0005922cellular_componentconnexin complex
C0007154biological_processcell communication
C0007267biological_processcell-cell signaling
C0007605biological_processsensory perception of sound
C0010644biological_processcell communication by electrical coupling
C0016020cellular_componentmembrane
C0016264biological_processgap junction assembly
C0042802molecular_functionidentical protein binding
C0046872molecular_functionmetal ion binding
C0055085biological_processtransmembrane transport
C0070161cellular_componentanchoring junction
C1903763molecular_functiongap junction channel activity involved in cell communication by electrical coupling
C1990349biological_processgap junction-mediated intercellular transport
D0005243molecular_functiongap junction channel activity
D0005509molecular_functioncalcium ion binding
D0005515molecular_functionprotein binding
D0005793cellular_componentendoplasmic reticulum-Golgi intermediate compartment
D0005886cellular_componentplasma membrane
D0005921cellular_componentgap junction
D0005922cellular_componentconnexin complex
D0007154biological_processcell communication
D0007267biological_processcell-cell signaling
D0007605biological_processsensory perception of sound
D0010644biological_processcell communication by electrical coupling
D0016020cellular_componentmembrane
D0016264biological_processgap junction assembly
D0042802molecular_functionidentical protein binding
D0046872molecular_functionmetal ion binding
D0055085biological_processtransmembrane transport
D0070161cellular_componentanchoring junction
D1903763molecular_functiongap junction channel activity involved in cell communication by electrical coupling
D1990349biological_processgap junction-mediated intercellular transport
E0005243molecular_functiongap junction channel activity
E0005509molecular_functioncalcium ion binding
E0005515molecular_functionprotein binding
E0005793cellular_componentendoplasmic reticulum-Golgi intermediate compartment
E0005886cellular_componentplasma membrane
E0005921cellular_componentgap junction
E0005922cellular_componentconnexin complex
E0007154biological_processcell communication
E0007267biological_processcell-cell signaling
E0007605biological_processsensory perception of sound
E0010644biological_processcell communication by electrical coupling
E0016020cellular_componentmembrane
E0016264biological_processgap junction assembly
E0042802molecular_functionidentical protein binding
E0046872molecular_functionmetal ion binding
E0055085biological_processtransmembrane transport
E0070161cellular_componentanchoring junction
E1903763molecular_functiongap junction channel activity involved in cell communication by electrical coupling
E1990349biological_processgap junction-mediated intercellular transport
F0005243molecular_functiongap junction channel activity
F0005509molecular_functioncalcium ion binding
F0005515molecular_functionprotein binding
F0005793cellular_componentendoplasmic reticulum-Golgi intermediate compartment
F0005886cellular_componentplasma membrane
F0005921cellular_componentgap junction
F0005922cellular_componentconnexin complex
F0007154biological_processcell communication
F0007267biological_processcell-cell signaling
F0007605biological_processsensory perception of sound
F0010644biological_processcell communication by electrical coupling
F0016020cellular_componentmembrane
F0016264biological_processgap junction assembly
F0042802molecular_functionidentical protein binding
F0046872molecular_functionmetal ion binding
F0055085biological_processtransmembrane transport
F0070161cellular_componentanchoring junction
F1903763molecular_functiongap junction channel activity involved in cell communication by electrical coupling
F1990349biological_processgap junction-mediated intercellular transport
G0005243molecular_functiongap junction channel activity
G0005509molecular_functioncalcium ion binding
G0005515molecular_functionprotein binding
G0005793cellular_componentendoplasmic reticulum-Golgi intermediate compartment
G0005886cellular_componentplasma membrane
G0005921cellular_componentgap junction
G0005922cellular_componentconnexin complex
G0007154biological_processcell communication
G0007267biological_processcell-cell signaling
G0007605biological_processsensory perception of sound
G0010644biological_processcell communication by electrical coupling
G0016020cellular_componentmembrane
G0016264biological_processgap junction assembly
G0042802molecular_functionidentical protein binding
G0046872molecular_functionmetal ion binding
G0055085biological_processtransmembrane transport
G0070161cellular_componentanchoring junction
G1903763molecular_functiongap junction channel activity involved in cell communication by electrical coupling
G1990349biological_processgap junction-mediated intercellular transport
H0005243molecular_functiongap junction channel activity
H0005509molecular_functioncalcium ion binding
H0005515molecular_functionprotein binding
H0005793cellular_componentendoplasmic reticulum-Golgi intermediate compartment
H0005886cellular_componentplasma membrane
H0005921cellular_componentgap junction
H0005922cellular_componentconnexin complex
H0007154biological_processcell communication
H0007267biological_processcell-cell signaling
H0007605biological_processsensory perception of sound
H0010644biological_processcell communication by electrical coupling
H0016020cellular_componentmembrane
H0016264biological_processgap junction assembly
H0042802molecular_functionidentical protein binding
H0046872molecular_functionmetal ion binding
H0055085biological_processtransmembrane transport
H0070161cellular_componentanchoring junction
H1903763molecular_functiongap junction channel activity involved in cell communication by electrical coupling
H1990349biological_processgap junction-mediated intercellular transport
I0005243molecular_functiongap junction channel activity
I0005509molecular_functioncalcium ion binding
I0005515molecular_functionprotein binding
I0005793cellular_componentendoplasmic reticulum-Golgi intermediate compartment
I0005886cellular_componentplasma membrane
I0005921cellular_componentgap junction
I0005922cellular_componentconnexin complex
I0007154biological_processcell communication
I0007267biological_processcell-cell signaling
I0007605biological_processsensory perception of sound
I0010644biological_processcell communication by electrical coupling
I0016020cellular_componentmembrane
I0016264biological_processgap junction assembly
I0042802molecular_functionidentical protein binding
I0046872molecular_functionmetal ion binding
I0055085biological_processtransmembrane transport
I0070161cellular_componentanchoring junction
I1903763molecular_functiongap junction channel activity involved in cell communication by electrical coupling
I1990349biological_processgap junction-mediated intercellular transport
J0005243molecular_functiongap junction channel activity
J0005509molecular_functioncalcium ion binding
J0005515molecular_functionprotein binding
J0005793cellular_componentendoplasmic reticulum-Golgi intermediate compartment
J0005886cellular_componentplasma membrane
J0005921cellular_componentgap junction
J0005922cellular_componentconnexin complex
J0007154biological_processcell communication
J0007267biological_processcell-cell signaling
J0007605biological_processsensory perception of sound
J0010644biological_processcell communication by electrical coupling
J0016020cellular_componentmembrane
J0016264biological_processgap junction assembly
J0042802molecular_functionidentical protein binding
J0046872molecular_functionmetal ion binding
J0055085biological_processtransmembrane transport
J0070161cellular_componentanchoring junction
J1903763molecular_functiongap junction channel activity involved in cell communication by electrical coupling
J1990349biological_processgap junction-mediated intercellular transport
K0005243molecular_functiongap junction channel activity
K0005509molecular_functioncalcium ion binding
K0005515molecular_functionprotein binding
K0005793cellular_componentendoplasmic reticulum-Golgi intermediate compartment
K0005886cellular_componentplasma membrane
K0005921cellular_componentgap junction
K0005922cellular_componentconnexin complex
K0007154biological_processcell communication
K0007267biological_processcell-cell signaling
K0007605biological_processsensory perception of sound
K0010644biological_processcell communication by electrical coupling
K0016020cellular_componentmembrane
K0016264biological_processgap junction assembly
K0042802molecular_functionidentical protein binding
K0046872molecular_functionmetal ion binding
K0055085biological_processtransmembrane transport
K0070161cellular_componentanchoring junction
K1903763molecular_functiongap junction channel activity involved in cell communication by electrical coupling
K1990349biological_processgap junction-mediated intercellular transport
L0005243molecular_functiongap junction channel activity
L0005509molecular_functioncalcium ion binding
L0005515molecular_functionprotein binding
L0005793cellular_componentendoplasmic reticulum-Golgi intermediate compartment
L0005886cellular_componentplasma membrane
L0005921cellular_componentgap junction
L0005922cellular_componentconnexin complex
L0007154biological_processcell communication
L0007267biological_processcell-cell signaling
L0007605biological_processsensory perception of sound
L0010644biological_processcell communication by electrical coupling
L0016020cellular_componentmembrane
L0016264biological_processgap junction assembly
L0042802molecular_functionidentical protein binding
L0046872molecular_functionmetal ion binding
L0055085biological_processtransmembrane transport
L0070161cellular_componentanchoring junction
L1903763molecular_functiongap junction channel activity involved in cell communication by electrical coupling
L1990349biological_processgap junction-mediated intercellular transport
Functional Information from PROSITE/UniProt
site_idPS00407
Number of Residues14
DetailsCONNEXINS_1 Connexins signature 1. CNTlQPGCknVCYD
ChainResidueDetails
ACYS53-ASP66

site_idPS00408
Number of Residues17
DetailsCONNEXINS_2 Connexins signature 2. Cnaw.PCpntVDCFVSRP
ChainResidueDetails
ACYS169-PRO185

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues132
DetailsINTRAMEM: INTRAMEM => ECO:0000269|PubMed:26753910
ChainResidueDetails
AASP2-VAL13
JASP2-VAL13
KASP2-VAL13
LASP2-VAL13
BASP2-VAL13
CASP2-VAL13
DASP2-VAL13
EASP2-VAL13
FASP2-VAL13
GASP2-VAL13
HASP2-VAL13
IASP2-VAL13

site_idSWS_FT_FI2
Number of Residues732
DetailsTOPO_DOM: Cytoplasmic => ECO:0000269|PubMed:26753910
ChainResidueDetails
AASN14-ILE20
DASN14-ILE20
DVAL95-THR135
DCYS211-VAL226
EASN14-ILE20
EVAL95-THR135
ECYS211-VAL226
FASN14-ILE20
FVAL95-THR135
FCYS211-VAL226
GASN14-ILE20
AVAL95-THR135
GVAL95-THR135
GCYS211-VAL226
HASN14-ILE20
HVAL95-THR135
HCYS211-VAL226
IASN14-ILE20
IVAL95-THR135
ICYS211-VAL226
JASN14-ILE20
JVAL95-THR135
ACYS211-VAL226
JCYS211-VAL226
KASN14-ILE20
KVAL95-THR135
KCYS211-VAL226
LASN14-ILE20
LVAL95-THR135
LCYS211-VAL226
BASN14-ILE20
BVAL95-THR135
BCYS211-VAL226
CASN14-ILE20
CVAL95-THR135
CCYS211-VAL226

site_idSWS_FT_FI3
Number of Residues948
DetailsTRANSMEM: Helical => ECO:0000269|PubMed:26753910
ChainResidueDetails
AGLY21-ALA40
CILE74-HIS94
CTYR136-VAL156
CVAL190-LEU210
DGLY21-ALA40
DILE74-HIS94
DTYR136-VAL156
DVAL190-LEU210
EGLY21-ALA40
EILE74-HIS94
ETYR136-VAL156
AILE74-HIS94
EVAL190-LEU210
FGLY21-ALA40
FILE74-HIS94
FTYR136-VAL156
FVAL190-LEU210
GGLY21-ALA40
GILE74-HIS94
GTYR136-VAL156
GVAL190-LEU210
HGLY21-ALA40
ATYR136-VAL156
HILE74-HIS94
HTYR136-VAL156
HVAL190-LEU210
IGLY21-ALA40
IILE74-HIS94
ITYR136-VAL156
IVAL190-LEU210
JGLY21-ALA40
JILE74-HIS94
JTYR136-VAL156
AVAL190-LEU210
JVAL190-LEU210
KGLY21-ALA40
KILE74-HIS94
KTYR136-VAL156
KVAL190-LEU210
LGLY21-ALA40
LILE74-HIS94
LTYR136-VAL156
LVAL190-LEU210
BGLY21-ALA40
BILE74-HIS94
BTYR136-VAL156
BVAL190-LEU210
CGLY21-ALA40

site_idSWS_FT_FI4
Number of Residues768
DetailsTOPO_DOM: Extracellular => ECO:0000269|PubMed:26753910
ChainResidueDetails
ALYS41-HIS73
EMET157-THR189
FLYS41-HIS73
FMET157-THR189
GLYS41-HIS73
GMET157-THR189
HLYS41-HIS73
HMET157-THR189
ILYS41-HIS73
IMET157-THR189
JLYS41-HIS73
AMET157-THR189
JMET157-THR189
KLYS41-HIS73
KMET157-THR189
LLYS41-HIS73
LMET157-THR189
BLYS41-HIS73
BMET157-THR189
CLYS41-HIS73
CMET157-THR189
DLYS41-HIS73
DMET157-THR189
ELYS41-HIS73

site_idSWS_FT_FI5
Number of Residues12
DetailsBINDING: in other chain => ECO:0000305|PubMed:26753910, ECO:0007744|PDB:5ER7
ChainResidueDetails
AGLU42
JGLU42
KGLU42
LGLU42
BGLU42
CGLU42
DGLU42
EGLU42
FGLU42
GGLU42
HGLU42
IGLU42

site_idSWS_FT_FI6
Number of Residues24
DetailsBINDING: BINDING => ECO:0000305|PubMed:26753910, ECO:0007744|PDB:5ER7
ChainResidueDetails
AGLY45
EGLU47
FGLY45
FGLU47
GGLY45
GGLU47
HGLY45
HGLU47
IGLY45
IGLU47
JGLY45
AGLU47
JGLU47
KGLY45
KGLU47
LGLY45
LGLU47
BGLY45
BGLU47
CGLY45
CGLU47
DGLY45
DGLU47
EGLY45

237735

PDB entries from 2025-06-18

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