8Q3V

Cryo-EM structure of the methanogenic Na+ translocating N5-methyl-H4MPT:CoM methyltransferase complex

Functional Information from GO Data

Chain	GOid	namespace	contents
a	0005886	cellular_component	plasma membrane
a	0006730	biological_process	one-carbon metabolic process
a	0006814	biological_process	sodium ion transport
a	0008168	molecular_function	methyltransferase activity
a	0015948	biological_process	methanogenesis
a	0016020	cellular_component	membrane
a	0016740	molecular_function	transferase activity
a	0019386	biological_process	methanogenesis, from carbon dioxide
a	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
a	0032259	biological_process	methylation
a	0034708	cellular_component	methyltransferase complex
a	0035725	biological_process	sodium ion transmembrane transport
a	0050897	molecular_function	cobalt ion binding
A	0005886	cellular_component	plasma membrane
A	0006730	biological_process	one-carbon metabolic process
A	0006814	biological_process	sodium ion transport
A	0008168	molecular_function	methyltransferase activity
A	0015948	biological_process	methanogenesis
A	0016020	cellular_component	membrane
A	0016740	molecular_function	transferase activity
A	0019386	biological_process	methanogenesis, from carbon dioxide
A	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
A	0032259	biological_process	methylation
A	0034708	cellular_component	methyltransferase complex
A	0035725	biological_process	sodium ion transmembrane transport
A	0050897	molecular_function	cobalt ion binding
b	0005886	cellular_component	plasma membrane
b	0006730	biological_process	one-carbon metabolic process
b	0008168	molecular_function	methyltransferase activity
b	0015948	biological_process	methanogenesis
b	0016020	cellular_component	membrane
b	0016740	molecular_function	transferase activity
b	0019386	biological_process	methanogenesis, from carbon dioxide
b	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
b	0032259	biological_process	methylation
b	0034708	cellular_component	methyltransferase complex
b	0035725	biological_process	sodium ion transmembrane transport
B	0005886	cellular_component	plasma membrane
B	0006730	biological_process	one-carbon metabolic process
B	0008168	molecular_function	methyltransferase activity
B	0015948	biological_process	methanogenesis
B	0016020	cellular_component	membrane
B	0016740	molecular_function	transferase activity
B	0019386	biological_process	methanogenesis, from carbon dioxide
B	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
B	0032259	biological_process	methylation
B	0034708	cellular_component	methyltransferase complex
B	0035725	biological_process	sodium ion transmembrane transport
c	0005886	cellular_component	plasma membrane
c	0006730	biological_process	one-carbon metabolic process
c	0008168	molecular_function	methyltransferase activity
c	0015948	biological_process	methanogenesis
c	0016020	cellular_component	membrane
c	0016740	molecular_function	transferase activity
c	0019386	biological_process	methanogenesis, from carbon dioxide
c	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
c	0032259	biological_process	methylation
c	0034708	cellular_component	methyltransferase complex
c	0035725	biological_process	sodium ion transmembrane transport
C	0005886	cellular_component	plasma membrane
C	0006730	biological_process	one-carbon metabolic process
C	0008168	molecular_function	methyltransferase activity
C	0015948	biological_process	methanogenesis
C	0016020	cellular_component	membrane
C	0016740	molecular_function	transferase activity
C	0019386	biological_process	methanogenesis, from carbon dioxide
C	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
C	0032259	biological_process	methylation
C	0034708	cellular_component	methyltransferase complex
C	0035725	biological_process	sodium ion transmembrane transport
d	0005737	cellular_component	cytoplasm
d	0005886	cellular_component	plasma membrane
d	0006730	biological_process	one-carbon metabolic process
d	0006814	biological_process	sodium ion transport
d	0008168	molecular_function	methyltransferase activity
d	0012506	cellular_component	vesicle membrane
d	0015948	biological_process	methanogenesis
d	0016020	cellular_component	membrane
d	0016740	molecular_function	transferase activity
d	0019386	biological_process	methanogenesis, from carbon dioxide
d	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
d	0032259	biological_process	methylation
d	0034708	cellular_component	methyltransferase complex
d	0035725	biological_process	sodium ion transmembrane transport
D	0005737	cellular_component	cytoplasm
D	0005886	cellular_component	plasma membrane
D	0006730	biological_process	one-carbon metabolic process
D	0006814	biological_process	sodium ion transport
D	0008168	molecular_function	methyltransferase activity
D	0012506	cellular_component	vesicle membrane
D	0015948	biological_process	methanogenesis
D	0016020	cellular_component	membrane
D	0016740	molecular_function	transferase activity
D	0019386	biological_process	methanogenesis, from carbon dioxide
D	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
D	0032259	biological_process	methylation
D	0034708	cellular_component	methyltransferase complex
D	0035725	biological_process	sodium ion transmembrane transport
e	0005737	cellular_component	cytoplasm
e	0005886	cellular_component	plasma membrane
e	0006730	biological_process	one-carbon metabolic process
e	0006814	biological_process	sodium ion transport
e	0008168	molecular_function	methyltransferase activity
e	0012506	cellular_component	vesicle membrane
e	0015948	biological_process	methanogenesis
e	0016020	cellular_component	membrane
e	0016740	molecular_function	transferase activity
e	0019386	biological_process	methanogenesis, from carbon dioxide
e	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
e	0032259	biological_process	methylation
e	0034708	cellular_component	methyltransferase complex
e	0035725	biological_process	sodium ion transmembrane transport
E	0005737	cellular_component	cytoplasm
E	0005886	cellular_component	plasma membrane
E	0006730	biological_process	one-carbon metabolic process
E	0006814	biological_process	sodium ion transport
E	0008168	molecular_function	methyltransferase activity
E	0012506	cellular_component	vesicle membrane
E	0015948	biological_process	methanogenesis
E	0016020	cellular_component	membrane
E	0016740	molecular_function	transferase activity
E	0019386	biological_process	methanogenesis, from carbon dioxide
E	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
E	0032259	biological_process	methylation
E	0034708	cellular_component	methyltransferase complex
E	0035725	biological_process	sodium ion transmembrane transport
f	0005886	cellular_component	plasma membrane
f	0006730	biological_process	one-carbon metabolic process
f	0008168	molecular_function	methyltransferase activity
f	0015948	biological_process	methanogenesis
f	0016020	cellular_component	membrane
f	0016740	molecular_function	transferase activity
f	0019386	biological_process	methanogenesis, from carbon dioxide
f	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
f	0032259	biological_process	methylation
f	0034708	cellular_component	methyltransferase complex
f	0035725	biological_process	sodium ion transmembrane transport
F	0005886	cellular_component	plasma membrane
F	0006730	biological_process	one-carbon metabolic process
F	0008168	molecular_function	methyltransferase activity
F	0015948	biological_process	methanogenesis
F	0016020	cellular_component	membrane
F	0016740	molecular_function	transferase activity
F	0019386	biological_process	methanogenesis, from carbon dioxide
F	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
F	0032259	biological_process	methylation
F	0034708	cellular_component	methyltransferase complex
F	0035725	biological_process	sodium ion transmembrane transport
g	0005886	cellular_component	plasma membrane
g	0006730	biological_process	one-carbon metabolic process
g	0008168	molecular_function	methyltransferase activity
g	0015948	biological_process	methanogenesis
g	0016020	cellular_component	membrane
g	0016740	molecular_function	transferase activity
g	0019386	biological_process	methanogenesis, from carbon dioxide
g	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
g	0032259	biological_process	methylation
g	0034708	cellular_component	methyltransferase complex
g	0035725	biological_process	sodium ion transmembrane transport
G	0005886	cellular_component	plasma membrane
G	0006730	biological_process	one-carbon metabolic process
G	0008168	molecular_function	methyltransferase activity
G	0015948	biological_process	methanogenesis
G	0016020	cellular_component	membrane
G	0016740	molecular_function	transferase activity
G	0019386	biological_process	methanogenesis, from carbon dioxide
G	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
G	0032259	biological_process	methylation
G	0034708	cellular_component	methyltransferase complex
G	0035725	biological_process	sodium ion transmembrane transport
Q	0005886	cellular_component	plasma membrane
Q	0006730	biological_process	one-carbon metabolic process
Q	0006814	biological_process	sodium ion transport
Q	0008168	molecular_function	methyltransferase activity
Q	0015948	biological_process	methanogenesis
Q	0016020	cellular_component	membrane
Q	0016740	molecular_function	transferase activity
Q	0019386	biological_process	methanogenesis, from carbon dioxide
Q	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
Q	0032259	biological_process	methylation
Q	0034708	cellular_component	methyltransferase complex
Q	0035725	biological_process	sodium ion transmembrane transport
Q	0050897	molecular_function	cobalt ion binding
R	0005886	cellular_component	plasma membrane
R	0006730	biological_process	one-carbon metabolic process
R	0008168	molecular_function	methyltransferase activity
R	0015948	biological_process	methanogenesis
R	0016020	cellular_component	membrane
R	0016740	molecular_function	transferase activity
R	0019386	biological_process	methanogenesis, from carbon dioxide
R	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
R	0032259	biological_process	methylation
R	0034708	cellular_component	methyltransferase complex
R	0035725	biological_process	sodium ion transmembrane transport
S	0005886	cellular_component	plasma membrane
S	0006730	biological_process	one-carbon metabolic process
S	0008168	molecular_function	methyltransferase activity
S	0015948	biological_process	methanogenesis
S	0016020	cellular_component	membrane
S	0016740	molecular_function	transferase activity
S	0019386	biological_process	methanogenesis, from carbon dioxide
S	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
S	0032259	biological_process	methylation
S	0034708	cellular_component	methyltransferase complex
S	0035725	biological_process	sodium ion transmembrane transport
T	0005737	cellular_component	cytoplasm
T	0005886	cellular_component	plasma membrane
T	0006730	biological_process	one-carbon metabolic process
T	0006814	biological_process	sodium ion transport
T	0008168	molecular_function	methyltransferase activity
T	0012506	cellular_component	vesicle membrane
T	0015948	biological_process	methanogenesis
T	0016020	cellular_component	membrane
T	0016740	molecular_function	transferase activity
T	0019386	biological_process	methanogenesis, from carbon dioxide
T	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
T	0032259	biological_process	methylation
T	0034708	cellular_component	methyltransferase complex
T	0035725	biological_process	sodium ion transmembrane transport
U	0005737	cellular_component	cytoplasm
U	0005886	cellular_component	plasma membrane
U	0006730	biological_process	one-carbon metabolic process
U	0006814	biological_process	sodium ion transport
U	0008168	molecular_function	methyltransferase activity
U	0012506	cellular_component	vesicle membrane
U	0015948	biological_process	methanogenesis
U	0016020	cellular_component	membrane
U	0016740	molecular_function	transferase activity
U	0019386	biological_process	methanogenesis, from carbon dioxide
U	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
U	0032259	biological_process	methylation
U	0034708	cellular_component	methyltransferase complex
U	0035725	biological_process	sodium ion transmembrane transport
V	0005886	cellular_component	plasma membrane
V	0006730	biological_process	one-carbon metabolic process
V	0008168	molecular_function	methyltransferase activity
V	0015948	biological_process	methanogenesis
V	0016020	cellular_component	membrane
V	0016740	molecular_function	transferase activity
V	0019386	biological_process	methanogenesis, from carbon dioxide
V	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
V	0032259	biological_process	methylation
V	0034708	cellular_component	methyltransferase complex
V	0035725	biological_process	sodium ion transmembrane transport
W	0005886	cellular_component	plasma membrane
W	0006730	biological_process	one-carbon metabolic process
W	0008168	molecular_function	methyltransferase activity
W	0015948	biological_process	methanogenesis
W	0016020	cellular_component	membrane
W	0016740	molecular_function	transferase activity
W	0019386	biological_process	methanogenesis, from carbon dioxide
W	0030269	molecular_function	tetrahydromethanopterin S-methyltransferase activity
W	0032259	biological_process	methylation
W	0034708	cellular_component	methyltransferase complex
W	0035725	biological_process	sodium ion transmembrane transport

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	60
Details	TRANSMEM: Helical => ECO:0000255

Chain	Residue	Details
G	ILE52-PHE72
e	VAL87-VAL107
e	GLY140-GLY160
e	PHE161-ILE181
e	TYR234-THR254
e	ILE255-ILE275
U	MET4-ALA24
U	GLY60-MET80
U	VAL87-VAL107
U	GLY140-GLY160
U	PHE161-ILE181
g	ILE52-PHE72
U	TYR234-THR254
U	ILE255-ILE275
S	LEU163-ILE183
S	PHE209-VAL229
S	SER230-GLU250
W	ILE52-PHE72
E	GLY140-GLY160
E	PHE161-ILE181
E	TYR234-THR254
E	ILE255-ILE275
e	MET4-ALA24
e	GLY60-MET80

---

site_id	SWS_FT_FI2
Number of Residues	54
Details	TRANSMEM: Helical => ECO:0000255|HAMAP-Rule:MF_01093

Chain	Residue	Details
A	VAL219-LEU237
a	VAL219-LEU237
Q	VAL219-LEU237

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	TOPO_DOM: Extracellular => ECO:0000255|HAMAP-Rule:MF_01093

Chain	Residue	Details
A	VAL238
a	VAL238
Q	VAL238

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	BINDING: BINDING => ECO:0000305

Chain	Residue	Details
A	HIS84
a	HIS84
Q	HIS84

---