8PY2

Cryo-EM structure of the human BRISC dimer complex bound to compound JMS-175-2

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000151	cellular_component	ubiquitin ligase complex
A	0000152	cellular_component	nuclear ubiquitin ligase complex
A	0000922	cellular_component	spindle pole
A	0004843	molecular_function	cysteine-type deubiquitinase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006281	biological_process	DNA repair
A	0006282	biological_process	regulation of DNA repair
A	0006302	biological_process	double-strand break repair
A	0006325	biological_process	chromatin organization
A	0006508	biological_process	proteolysis
A	0006974	biological_process	DNA damage response
A	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
A	0008233	molecular_function	peptidase activity
A	0008237	molecular_function	metallopeptidase activity
A	0010165	biological_process	response to X-ray
A	0010212	biological_process	response to ionizing radiation
A	0016579	biological_process	protein deubiquitination
A	0016787	molecular_function	hydrolase activity
A	0030234	molecular_function	enzyme regulator activity
A	0031593	molecular_function	polyubiquitin modification-dependent protein binding
A	0034516	biological_process	response to vitamin B6
A	0044818	biological_process	mitotic G2/M transition checkpoint
A	0045739	biological_process	positive regulation of DNA repair
A	0046872	molecular_function	metal ion binding
A	0051301	biological_process	cell division
A	0061578	molecular_function	K63-linked deubiquitinase activity
A	0070531	cellular_component	BRCA1-A complex
A	0070536	biological_process	protein K63-linked deubiquitination
A	0070552	cellular_component	BRISC complex
A	0071479	biological_process	cellular response to ionizing radiation
A	0140492	molecular_function	metal-dependent deubiquitinase activity
A	0140861	biological_process	DNA repair-dependent chromatin remodeling
A	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
A	2000001	biological_process	regulation of DNA damage checkpoint
C	0000151	cellular_component	ubiquitin ligase complex
C	0000152	cellular_component	nuclear ubiquitin ligase complex
C	0000922	cellular_component	spindle pole
C	0004843	molecular_function	cysteine-type deubiquitinase activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006281	biological_process	DNA repair
C	0006282	biological_process	regulation of DNA repair
C	0006302	biological_process	double-strand break repair
C	0006325	biological_process	chromatin organization
C	0006508	biological_process	proteolysis
C	0006974	biological_process	DNA damage response
C	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
C	0008233	molecular_function	peptidase activity
C	0008237	molecular_function	metallopeptidase activity
C	0010165	biological_process	response to X-ray
C	0010212	biological_process	response to ionizing radiation
C	0016579	biological_process	protein deubiquitination
C	0016787	molecular_function	hydrolase activity
C	0030234	molecular_function	enzyme regulator activity
C	0031593	molecular_function	polyubiquitin modification-dependent protein binding
C	0034516	biological_process	response to vitamin B6
C	0044818	biological_process	mitotic G2/M transition checkpoint
C	0045739	biological_process	positive regulation of DNA repair
C	0046872	molecular_function	metal ion binding
C	0051301	biological_process	cell division
C	0061578	molecular_function	K63-linked deubiquitinase activity
C	0070531	cellular_component	BRCA1-A complex
C	0070536	biological_process	protein K63-linked deubiquitination
C	0070552	cellular_component	BRISC complex
C	0071479	biological_process	cellular response to ionizing radiation
C	0140492	molecular_function	metal-dependent deubiquitinase activity
C	0140861	biological_process	DNA repair-dependent chromatin remodeling
C	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
C	2000001	biological_process	regulation of DNA damage checkpoint
E	0000152	cellular_component	nuclear ubiquitin ligase complex
E	0000268	molecular_function	peroxisome targeting sequence binding
E	0005164	molecular_function	tumor necrosis factor receptor binding
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006281	biological_process	DNA repair
E	0006282	biological_process	regulation of DNA repair
E	0006302	biological_process	double-strand break repair
E	0006325	biological_process	chromatin organization
E	0006915	biological_process	apoptotic process
E	0006974	biological_process	DNA damage response
E	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
E	0007165	biological_process	signal transduction
E	0010212	biological_process	response to ionizing radiation
E	0031593	molecular_function	polyubiquitin modification-dependent protein binding
E	0034516	biological_process	response to vitamin B6
E	0043066	biological_process	negative regulation of apoptotic process
E	0044818	biological_process	mitotic G2/M transition checkpoint
E	0045739	biological_process	positive regulation of DNA repair
E	0051301	biological_process	cell division
E	0070531	cellular_component	BRCA1-A complex
E	0070536	biological_process	protein K63-linked deubiquitination
E	0070552	cellular_component	BRISC complex
E	0071479	biological_process	cellular response to ionizing radiation
E	2000001	biological_process	regulation of DNA damage checkpoint
F	0000152	cellular_component	nuclear ubiquitin ligase complex
F	0000268	molecular_function	peroxisome targeting sequence binding
F	0005164	molecular_function	tumor necrosis factor receptor binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006281	biological_process	DNA repair
F	0006282	biological_process	regulation of DNA repair
F	0006302	biological_process	double-strand break repair
F	0006325	biological_process	chromatin organization
F	0006915	biological_process	apoptotic process
F	0006974	biological_process	DNA damage response
F	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
F	0007165	biological_process	signal transduction
F	0010212	biological_process	response to ionizing radiation
F	0031593	molecular_function	polyubiquitin modification-dependent protein binding
F	0034516	biological_process	response to vitamin B6
F	0043066	biological_process	negative regulation of apoptotic process
F	0044818	biological_process	mitotic G2/M transition checkpoint
F	0045739	biological_process	positive regulation of DNA repair
F	0051301	biological_process	cell division
F	0070531	cellular_component	BRCA1-A complex
F	0070536	biological_process	protein K63-linked deubiquitination
F	0070552	cellular_component	BRISC complex
F	0071479	biological_process	cellular response to ionizing radiation
F	2000001	biological_process	regulation of DNA damage checkpoint
G	0000151	cellular_component	ubiquitin ligase complex
G	0000152	cellular_component	nuclear ubiquitin ligase complex
G	0000922	cellular_component	spindle pole
G	0004843	molecular_function	cysteine-type deubiquitinase activity
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006281	biological_process	DNA repair
G	0006282	biological_process	regulation of DNA repair
G	0006302	biological_process	double-strand break repair
G	0006325	biological_process	chromatin organization
G	0006508	biological_process	proteolysis
G	0006974	biological_process	DNA damage response
G	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
G	0008233	molecular_function	peptidase activity
G	0008237	molecular_function	metallopeptidase activity
G	0010165	biological_process	response to X-ray
G	0010212	biological_process	response to ionizing radiation
G	0016579	biological_process	protein deubiquitination
G	0016787	molecular_function	hydrolase activity
G	0030234	molecular_function	enzyme regulator activity
G	0031593	molecular_function	polyubiquitin modification-dependent protein binding
G	0034516	biological_process	response to vitamin B6
G	0044818	biological_process	mitotic G2/M transition checkpoint
G	0045739	biological_process	positive regulation of DNA repair
G	0046872	molecular_function	metal ion binding
G	0051301	biological_process	cell division
G	0061578	molecular_function	K63-linked deubiquitinase activity
G	0070531	cellular_component	BRCA1-A complex
G	0070536	biological_process	protein K63-linked deubiquitination
G	0070552	cellular_component	BRISC complex
G	0071479	biological_process	cellular response to ionizing radiation
G	0140492	molecular_function	metal-dependent deubiquitinase activity
G	0140861	biological_process	DNA repair-dependent chromatin remodeling
G	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
G	2000001	biological_process	regulation of DNA damage checkpoint
I	0000151	cellular_component	ubiquitin ligase complex
I	0000152	cellular_component	nuclear ubiquitin ligase complex
I	0000922	cellular_component	spindle pole
I	0004843	molecular_function	cysteine-type deubiquitinase activity
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006281	biological_process	DNA repair
I	0006282	biological_process	regulation of DNA repair
I	0006302	biological_process	double-strand break repair
I	0006325	biological_process	chromatin organization
I	0006508	biological_process	proteolysis
I	0006974	biological_process	DNA damage response
I	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
I	0008233	molecular_function	peptidase activity
I	0008237	molecular_function	metallopeptidase activity
I	0010165	biological_process	response to X-ray
I	0010212	biological_process	response to ionizing radiation
I	0016579	biological_process	protein deubiquitination
I	0016787	molecular_function	hydrolase activity
I	0030234	molecular_function	enzyme regulator activity
I	0031593	molecular_function	polyubiquitin modification-dependent protein binding
I	0034516	biological_process	response to vitamin B6
I	0044818	biological_process	mitotic G2/M transition checkpoint
I	0045739	biological_process	positive regulation of DNA repair
I	0046872	molecular_function	metal ion binding
I	0051301	biological_process	cell division
I	0061578	molecular_function	K63-linked deubiquitinase activity
I	0070531	cellular_component	BRCA1-A complex
I	0070536	biological_process	protein K63-linked deubiquitination
I	0070552	cellular_component	BRISC complex
I	0071479	biological_process	cellular response to ionizing radiation
I	0140492	molecular_function	metal-dependent deubiquitinase activity
I	0140861	biological_process	DNA repair-dependent chromatin remodeling
I	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
I	2000001	biological_process	regulation of DNA damage checkpoint
K	0000152	cellular_component	nuclear ubiquitin ligase complex
K	0000268	molecular_function	peroxisome targeting sequence binding
K	0005164	molecular_function	tumor necrosis factor receptor binding
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006281	biological_process	DNA repair
K	0006282	biological_process	regulation of DNA repair
K	0006302	biological_process	double-strand break repair
K	0006325	biological_process	chromatin organization
K	0006915	biological_process	apoptotic process
K	0006974	biological_process	DNA damage response
K	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
K	0007165	biological_process	signal transduction
K	0010212	biological_process	response to ionizing radiation
K	0031593	molecular_function	polyubiquitin modification-dependent protein binding
K	0034516	biological_process	response to vitamin B6
K	0043066	biological_process	negative regulation of apoptotic process
K	0044818	biological_process	mitotic G2/M transition checkpoint
K	0045739	biological_process	positive regulation of DNA repair
K	0051301	biological_process	cell division
K	0070531	cellular_component	BRCA1-A complex
K	0070536	biological_process	protein K63-linked deubiquitination
K	0070552	cellular_component	BRISC complex
K	0071479	biological_process	cellular response to ionizing radiation
K	2000001	biological_process	regulation of DNA damage checkpoint
L	0000152	cellular_component	nuclear ubiquitin ligase complex
L	0000268	molecular_function	peroxisome targeting sequence binding
L	0005164	molecular_function	tumor necrosis factor receptor binding
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0006281	biological_process	DNA repair
L	0006282	biological_process	regulation of DNA repair
L	0006302	biological_process	double-strand break repair
L	0006325	biological_process	chromatin organization
L	0006915	biological_process	apoptotic process
L	0006974	biological_process	DNA damage response
L	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
L	0007165	biological_process	signal transduction
L	0010212	biological_process	response to ionizing radiation
L	0031593	molecular_function	polyubiquitin modification-dependent protein binding
L	0034516	biological_process	response to vitamin B6
L	0043066	biological_process	negative regulation of apoptotic process
L	0044818	biological_process	mitotic G2/M transition checkpoint
L	0045739	biological_process	positive regulation of DNA repair
L	0051301	biological_process	cell division
L	0070531	cellular_component	BRCA1-A complex
L	0070536	biological_process	protein K63-linked deubiquitination
L	0070552	cellular_component	BRISC complex
L	0071479	biological_process	cellular response to ionizing radiation
L	2000001	biological_process	regulation of DNA damage checkpoint
M	0045739	biological_process	positive regulation of DNA repair
M	0070531	cellular_component	BRCA1-A complex
M	0070552	cellular_component	BRISC complex
N	0045739	biological_process	positive regulation of DNA repair
N	0070531	cellular_component	BRCA1-A complex
N	0070552	cellular_component	BRISC complex
O	0045739	biological_process	positive regulation of DNA repair
O	0070531	cellular_component	BRCA1-A complex
O	0070552	cellular_component	BRISC complex
P	0045739	biological_process	positive regulation of DNA repair
P	0070531	cellular_component	BRCA1-A complex
P	0070552	cellular_component	BRISC complex

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	52
Details	Motif: {"description":"JAMM motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01182","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01182","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	28
Details	Region: {"description":"Important for interaction with SHMT2","evidences":[{"source":"PubMed","id":"24075985","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	84
Details	Region: {"description":"Important for interaction with BBRC36 and other subunits of the BRISC complex","evidences":[{"source":"PubMed","id":"26344097","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	468
Details	Region: {"description":"UEV-like 1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	356
Details	Region: {"description":"UEV-like 2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"PubMed","id":"19413330","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22223895","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	812
Details	Region: {"description":"VWFA-like"}

Chain

Residue

Details

---