8PRW

Cryo-EM structure of the yeast fatty acid synthase at 1.9 angstrom resolution

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0004312	molecular_function	fatty acid synthase activity
A	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
A	0004316	molecular_function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
A	0004321	molecular_function	fatty-acyl-CoA synthase activity
A	0005515	molecular_function	protein binding
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0005835	cellular_component	fatty acid synthase complex
A	0006633	biological_process	fatty acid biosynthetic process
A	0008897	molecular_function	holo-[acyl-carrier-protein] synthase activity
A	0016491	molecular_function	oxidoreductase activity
A	0016740	molecular_function	transferase activity
A	0016746	molecular_function	acyltransferase activity
A	0042759	biological_process	long-chain fatty acid biosynthetic process
A	0046872	molecular_function	metal ion binding
B	0000287	molecular_function	magnesium ion binding
B	0004312	molecular_function	fatty acid synthase activity
B	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
B	0004316	molecular_function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
B	0004321	molecular_function	fatty-acyl-CoA synthase activity
B	0005515	molecular_function	protein binding
B	0005739	cellular_component	mitochondrion
B	0005829	cellular_component	cytosol
B	0005835	cellular_component	fatty acid synthase complex
B	0006633	biological_process	fatty acid biosynthetic process
B	0008897	molecular_function	holo-[acyl-carrier-protein] synthase activity
B	0016491	molecular_function	oxidoreductase activity
B	0016740	molecular_function	transferase activity
B	0016746	molecular_function	acyltransferase activity
B	0042759	biological_process	long-chain fatty acid biosynthetic process
B	0046872	molecular_function	metal ion binding
C	0000287	molecular_function	magnesium ion binding
C	0004312	molecular_function	fatty acid synthase activity
C	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
C	0004316	molecular_function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
C	0004321	molecular_function	fatty-acyl-CoA synthase activity
C	0005515	molecular_function	protein binding
C	0005739	cellular_component	mitochondrion
C	0005829	cellular_component	cytosol
C	0005835	cellular_component	fatty acid synthase complex
C	0006633	biological_process	fatty acid biosynthetic process
C	0008897	molecular_function	holo-[acyl-carrier-protein] synthase activity
C	0016491	molecular_function	oxidoreductase activity
C	0016740	molecular_function	transferase activity
C	0016746	molecular_function	acyltransferase activity
C	0042759	biological_process	long-chain fatty acid biosynthetic process
C	0046872	molecular_function	metal ion binding
D	0000287	molecular_function	magnesium ion binding
D	0004312	molecular_function	fatty acid synthase activity
D	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
D	0004316	molecular_function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
D	0004321	molecular_function	fatty-acyl-CoA synthase activity
D	0005515	molecular_function	protein binding
D	0005739	cellular_component	mitochondrion
D	0005829	cellular_component	cytosol
D	0005835	cellular_component	fatty acid synthase complex
D	0006633	biological_process	fatty acid biosynthetic process
D	0008897	molecular_function	holo-[acyl-carrier-protein] synthase activity
D	0016491	molecular_function	oxidoreductase activity
D	0016740	molecular_function	transferase activity
D	0016746	molecular_function	acyltransferase activity
D	0042759	biological_process	long-chain fatty acid biosynthetic process
D	0046872	molecular_function	metal ion binding
E	0000287	molecular_function	magnesium ion binding
E	0004312	molecular_function	fatty acid synthase activity
E	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
E	0004316	molecular_function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
E	0004321	molecular_function	fatty-acyl-CoA synthase activity
E	0005515	molecular_function	protein binding
E	0005739	cellular_component	mitochondrion
E	0005829	cellular_component	cytosol
E	0005835	cellular_component	fatty acid synthase complex
E	0006633	biological_process	fatty acid biosynthetic process
E	0008897	molecular_function	holo-[acyl-carrier-protein] synthase activity
E	0016491	molecular_function	oxidoreductase activity
E	0016740	molecular_function	transferase activity
E	0016746	molecular_function	acyltransferase activity
E	0042759	biological_process	long-chain fatty acid biosynthetic process
E	0046872	molecular_function	metal ion binding
F	0000287	molecular_function	magnesium ion binding
F	0004312	molecular_function	fatty acid synthase activity
F	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
F	0004316	molecular_function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
F	0004321	molecular_function	fatty-acyl-CoA synthase activity
F	0005515	molecular_function	protein binding
F	0005739	cellular_component	mitochondrion
F	0005829	cellular_component	cytosol
F	0005835	cellular_component	fatty acid synthase complex
F	0006633	biological_process	fatty acid biosynthetic process
F	0008897	molecular_function	holo-[acyl-carrier-protein] synthase activity
F	0016491	molecular_function	oxidoreductase activity
F	0016740	molecular_function	transferase activity
F	0016746	molecular_function	acyltransferase activity
F	0042759	biological_process	long-chain fatty acid biosynthetic process
F	0046872	molecular_function	metal ion binding
G	0004312	molecular_function	fatty acid synthase activity
G	0004313	molecular_function	[acyl-carrier-protein] S-acetyltransferase activity
G	0004314	molecular_function	[acyl-carrier-protein] S-malonyltransferase activity
G	0004318	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADH) activity
G	0004321	molecular_function	fatty-acyl-CoA synthase activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005739	cellular_component	mitochondrion
G	0005811	cellular_component	lipid droplet
G	0005829	cellular_component	cytosol
G	0005835	cellular_component	fatty acid synthase complex
G	0006633	biological_process	fatty acid biosynthetic process
G	0016297	molecular_function	fatty acyl-[ACP] hydrolase activity
G	0016409	molecular_function	palmitoyltransferase activity
G	0016491	molecular_function	oxidoreductase activity
G	0016740	molecular_function	transferase activity
G	0016787	molecular_function	hydrolase activity
G	0016829	molecular_function	lyase activity
G	0019171	molecular_function	(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
G	0042759	biological_process	long-chain fatty acid biosynthetic process
G	0141148	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADPH) activity
H	0004312	molecular_function	fatty acid synthase activity
H	0004313	molecular_function	[acyl-carrier-protein] S-acetyltransferase activity
H	0004314	molecular_function	[acyl-carrier-protein] S-malonyltransferase activity
H	0004318	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADH) activity
H	0004321	molecular_function	fatty-acyl-CoA synthase activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005739	cellular_component	mitochondrion
H	0005811	cellular_component	lipid droplet
H	0005829	cellular_component	cytosol
H	0005835	cellular_component	fatty acid synthase complex
H	0006633	biological_process	fatty acid biosynthetic process
H	0016297	molecular_function	fatty acyl-[ACP] hydrolase activity
H	0016409	molecular_function	palmitoyltransferase activity
H	0016491	molecular_function	oxidoreductase activity
H	0016740	molecular_function	transferase activity
H	0016787	molecular_function	hydrolase activity
H	0016829	molecular_function	lyase activity
H	0019171	molecular_function	(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
H	0042759	biological_process	long-chain fatty acid biosynthetic process
H	0141148	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADPH) activity
I	0004312	molecular_function	fatty acid synthase activity
I	0004313	molecular_function	[acyl-carrier-protein] S-acetyltransferase activity
I	0004314	molecular_function	[acyl-carrier-protein] S-malonyltransferase activity
I	0004318	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADH) activity
I	0004321	molecular_function	fatty-acyl-CoA synthase activity
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005739	cellular_component	mitochondrion
I	0005811	cellular_component	lipid droplet
I	0005829	cellular_component	cytosol
I	0005835	cellular_component	fatty acid synthase complex
I	0006633	biological_process	fatty acid biosynthetic process
I	0016297	molecular_function	fatty acyl-[ACP] hydrolase activity
I	0016409	molecular_function	palmitoyltransferase activity
I	0016491	molecular_function	oxidoreductase activity
I	0016740	molecular_function	transferase activity
I	0016787	molecular_function	hydrolase activity
I	0016829	molecular_function	lyase activity
I	0019171	molecular_function	(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
I	0042759	biological_process	long-chain fatty acid biosynthetic process
I	0141148	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADPH) activity
J	0004312	molecular_function	fatty acid synthase activity
J	0004313	molecular_function	[acyl-carrier-protein] S-acetyltransferase activity
J	0004314	molecular_function	[acyl-carrier-protein] S-malonyltransferase activity
J	0004318	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADH) activity
J	0004321	molecular_function	fatty-acyl-CoA synthase activity
J	0005515	molecular_function	protein binding
J	0005737	cellular_component	cytoplasm
J	0005739	cellular_component	mitochondrion
J	0005811	cellular_component	lipid droplet
J	0005829	cellular_component	cytosol
J	0005835	cellular_component	fatty acid synthase complex
J	0006633	biological_process	fatty acid biosynthetic process
J	0016297	molecular_function	fatty acyl-[ACP] hydrolase activity
J	0016409	molecular_function	palmitoyltransferase activity
J	0016491	molecular_function	oxidoreductase activity
J	0016740	molecular_function	transferase activity
J	0016787	molecular_function	hydrolase activity
J	0016829	molecular_function	lyase activity
J	0019171	molecular_function	(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
J	0042759	biological_process	long-chain fatty acid biosynthetic process
J	0141148	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADPH) activity
K	0004312	molecular_function	fatty acid synthase activity
K	0004313	molecular_function	[acyl-carrier-protein] S-acetyltransferase activity
K	0004314	molecular_function	[acyl-carrier-protein] S-malonyltransferase activity
K	0004318	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADH) activity
K	0004321	molecular_function	fatty-acyl-CoA synthase activity
K	0005515	molecular_function	protein binding
K	0005737	cellular_component	cytoplasm
K	0005739	cellular_component	mitochondrion
K	0005811	cellular_component	lipid droplet
K	0005829	cellular_component	cytosol
K	0005835	cellular_component	fatty acid synthase complex
K	0006633	biological_process	fatty acid biosynthetic process
K	0016297	molecular_function	fatty acyl-[ACP] hydrolase activity
K	0016409	molecular_function	palmitoyltransferase activity
K	0016491	molecular_function	oxidoreductase activity
K	0016740	molecular_function	transferase activity
K	0016787	molecular_function	hydrolase activity
K	0016829	molecular_function	lyase activity
K	0019171	molecular_function	(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
K	0042759	biological_process	long-chain fatty acid biosynthetic process
K	0141148	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADPH) activity
L	0004312	molecular_function	fatty acid synthase activity
L	0004313	molecular_function	[acyl-carrier-protein] S-acetyltransferase activity
L	0004314	molecular_function	[acyl-carrier-protein] S-malonyltransferase activity
L	0004318	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADH) activity
L	0004321	molecular_function	fatty-acyl-CoA synthase activity
L	0005515	molecular_function	protein binding
L	0005737	cellular_component	cytoplasm
L	0005739	cellular_component	mitochondrion
L	0005811	cellular_component	lipid droplet
L	0005829	cellular_component	cytosol
L	0005835	cellular_component	fatty acid synthase complex
L	0006633	biological_process	fatty acid biosynthetic process
L	0016297	molecular_function	fatty acyl-[ACP] hydrolase activity
L	0016409	molecular_function	palmitoyltransferase activity
L	0016491	molecular_function	oxidoreductase activity
L	0016740	molecular_function	transferase activity
L	0016787	molecular_function	hydrolase activity
L	0016829	molecular_function	lyase activity
L	0019171	molecular_function	(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
L	0042759	biological_process	long-chain fatty acid biosynthetic process
L	0141148	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADPH) activity

Functional Information from PROSITE/UniProt

site_id	PS00012
Number of Residues	16
Details	PHOSPHOPANTETHEINE Phosphopantetheine attachment site. LVGGKSTVQNEILGDL

Chain	Residue	Details
A	LEU175-LEU190

---

site_id	PS00606
Number of Residues	17
Details	KS3_1 Ketosynthase family 3 (KS3) active site signature. GPIktPVgACATSveSV

Chain	Residue	Details
A	GLY1296-VAL1312

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	ACT_SITE: For acetyltransferase activity => ECO:0000250

Chain	Residue	Details
G	SER274
D	CYS1305
D	HIS1542
D	HIS1583
E	CYS1305
E	HIS1542
E	HIS1583
F	CYS1305
F	HIS1542
F	HIS1583
J	SER274
I	SER274
H	SER274
K	SER274
L	SER274
C	CYS1305
C	HIS1542
C	HIS1583

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	ACT_SITE: For malonyltransferase activity => ECO:0000250

Chain	Residue	Details
G	J8W1808
B	VAL1773
B	GLU1774
B	GLU1817
B	ARG1841
B	ILE1871
B	SER1872
B	HIS1873
C	ASP1772
C	VAL1773
C	GLU1774
J	J8W1808
C	GLU1817
C	ARG1841
C	ILE1871
C	SER1872
C	HIS1873
D	ASP1772
D	VAL1773
D	GLU1774
D	GLU1817
D	ARG1841
I	J8W1808
D	ILE1871
D	SER1872
D	HIS1873
E	ASP1772
E	VAL1773
E	GLU1774
E	GLU1817
E	ARG1841
E	ILE1871
E	SER1872
H	J8W1808
E	HIS1873
F	ASP1772
F	VAL1773
F	GLU1774
F	GLU1817
F	ARG1841
F	ILE1871
F	SER1872
F	HIS1873
K	J8W1808
L	J8W1808
A	SER1872
A	HIS1873
B	ASP1772

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	MOD_RES: N-acetylmethionine => ECO:0007744|PubMed:22814378

Chain	Residue	Details
G	MET1
E	SER1808
F	TYR1798
F	SER1808
J	MET1
I	MET1
H	MET1
K	MET1
L	MET1
D	TYR1798
D	SER1808
E	TYR1798

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:19779198

Chain	Residue	Details
G	THR733
J	THR733
I	THR733
H	THR733
K	THR733
L	THR733

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198

Chain	Residue	Details
G	SER1121
J	SER1121
I	SER1121
H	SER1121
K	SER1121
L	SER1121

---

site_id	SWS_FT_FI6
Number of Residues	12
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047

Chain	Residue	Details
G	LYS1364
L	LYS1364
B	SER523
J	LYS1364
D	SER523
I	LYS1364
F	SER523
H	LYS1364
K	LYS1364

---

site_id	SWS_FT_FI7
Number of Residues	12
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950

Chain	Residue	Details
A	SER958
E	SER1440
F	SER958
F	SER1440
A	SER1440
B	SER958
B	SER1440
C	SER958
C	SER1440
D	SER958
D	SER1440
E	SER958

---

site_id	SWS_FT_FI8
Number of Residues	12
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047

Chain	Residue	Details
A	LYS37
F	LYS37
B	LYS37
C	LYS37
D	LYS37
E	LYS37

---