8PIE

Crystal structure of the human nucleoside diphosphate kinase B domain in complex with the product AT-8500 formed by catalysis of compound AT-9010

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0001216	molecular_function	DNA-binding transcription activator activity
A	0001726	cellular_component	ruffle
A	0003677	molecular_function	DNA binding
A	0004518	molecular_function	nuclease activity
A	0004550	molecular_function	nucleoside diphosphate kinase activity
A	0004673	molecular_function	protein histidine kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005576	cellular_component	extracellular region
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005925	cellular_component	focal adhesion
A	0006183	biological_process	GTP biosynthetic process
A	0006228	biological_process	UTP biosynthetic process
A	0006241	biological_process	CTP biosynthetic process
A	0007155	biological_process	cell adhesion
A	0007229	biological_process	integrin-mediated signaling pathway
A	0009117	biological_process	nucleotide metabolic process
A	0009142	biological_process	nucleoside triphosphate biosynthetic process
A	0016301	molecular_function	kinase activity
A	0016740	molecular_function	transferase activity
A	0019003	molecular_function	GDP binding
A	0030027	cellular_component	lamellipodium
A	0034774	cellular_component	secretory granule lumen
A	0042304	biological_process	regulation of fatty acid biosynthetic process
A	0042802	molecular_function	identical protein binding
A	0042981	biological_process	regulation of apoptotic process
A	0043066	biological_process	negative regulation of apoptotic process
A	0045618	biological_process	positive regulation of keratinocyte differentiation
A	0045682	biological_process	regulation of epidermis development
A	0045893	biological_process	positive regulation of DNA-templated transcription
A	0045944	biological_process	positive regulation of transcription by RNA polymerase II
A	0046042	biological_process	ITP biosynthetic process
A	0046872	molecular_function	metal ion binding
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0050679	biological_process	positive regulation of epithelial cell proliferation
A	0051880	molecular_function	G-quadruplex DNA binding
A	0070062	cellular_component	extracellular exosome
A	0071944	cellular_component	cell periphery
A	0120225	molecular_function	coenzyme A binding
A	1904813	cellular_component	ficolin-1-rich granule lumen
A	2000144	biological_process	positive regulation of DNA-templated transcription initiation
B	0000166	molecular_function	nucleotide binding
B	0001216	molecular_function	DNA-binding transcription activator activity
B	0001726	cellular_component	ruffle
B	0003677	molecular_function	DNA binding
B	0004518	molecular_function	nuclease activity
B	0004550	molecular_function	nucleoside diphosphate kinase activity
B	0004673	molecular_function	protein histidine kinase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005576	cellular_component	extracellular region
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005925	cellular_component	focal adhesion
B	0006183	biological_process	GTP biosynthetic process
B	0006228	biological_process	UTP biosynthetic process
B	0006241	biological_process	CTP biosynthetic process
B	0007155	biological_process	cell adhesion
B	0007229	biological_process	integrin-mediated signaling pathway
B	0009117	biological_process	nucleotide metabolic process
B	0009142	biological_process	nucleoside triphosphate biosynthetic process
B	0016301	molecular_function	kinase activity
B	0016740	molecular_function	transferase activity
B	0019003	molecular_function	GDP binding
B	0030027	cellular_component	lamellipodium
B	0034774	cellular_component	secretory granule lumen
B	0042304	biological_process	regulation of fatty acid biosynthetic process
B	0042802	molecular_function	identical protein binding
B	0042981	biological_process	regulation of apoptotic process
B	0043066	biological_process	negative regulation of apoptotic process
B	0045618	biological_process	positive regulation of keratinocyte differentiation
B	0045682	biological_process	regulation of epidermis development
B	0045893	biological_process	positive regulation of DNA-templated transcription
B	0045944	biological_process	positive regulation of transcription by RNA polymerase II
B	0046042	biological_process	ITP biosynthetic process
B	0046872	molecular_function	metal ion binding
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0050679	biological_process	positive regulation of epithelial cell proliferation
B	0051880	molecular_function	G-quadruplex DNA binding
B	0070062	cellular_component	extracellular exosome
B	0071944	cellular_component	cell periphery
B	0120225	molecular_function	coenzyme A binding
B	1904813	cellular_component	ficolin-1-rich granule lumen
B	2000144	biological_process	positive regulation of DNA-templated transcription initiation
C	0000166	molecular_function	nucleotide binding
C	0001216	molecular_function	DNA-binding transcription activator activity
C	0001726	cellular_component	ruffle
C	0003677	molecular_function	DNA binding
C	0004518	molecular_function	nuclease activity
C	0004550	molecular_function	nucleoside diphosphate kinase activity
C	0004673	molecular_function	protein histidine kinase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005576	cellular_component	extracellular region
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005925	cellular_component	focal adhesion
C	0006183	biological_process	GTP biosynthetic process
C	0006228	biological_process	UTP biosynthetic process
C	0006241	biological_process	CTP biosynthetic process
C	0007155	biological_process	cell adhesion
C	0007229	biological_process	integrin-mediated signaling pathway
C	0009117	biological_process	nucleotide metabolic process
C	0009142	biological_process	nucleoside triphosphate biosynthetic process
C	0016301	molecular_function	kinase activity
C	0016740	molecular_function	transferase activity
C	0019003	molecular_function	GDP binding
C	0030027	cellular_component	lamellipodium
C	0034774	cellular_component	secretory granule lumen
C	0042304	biological_process	regulation of fatty acid biosynthetic process
C	0042802	molecular_function	identical protein binding
C	0042981	biological_process	regulation of apoptotic process
C	0043066	biological_process	negative regulation of apoptotic process
C	0045618	biological_process	positive regulation of keratinocyte differentiation
C	0045682	biological_process	regulation of epidermis development
C	0045893	biological_process	positive regulation of DNA-templated transcription
C	0045944	biological_process	positive regulation of transcription by RNA polymerase II
C	0046042	biological_process	ITP biosynthetic process
C	0046872	molecular_function	metal ion binding
C	0048471	cellular_component	perinuclear region of cytoplasm
C	0050679	biological_process	positive regulation of epithelial cell proliferation
C	0051880	molecular_function	G-quadruplex DNA binding
C	0070062	cellular_component	extracellular exosome
C	0071944	cellular_component	cell periphery
C	0120225	molecular_function	coenzyme A binding
C	1904813	cellular_component	ficolin-1-rich granule lumen
C	2000144	biological_process	positive regulation of DNA-templated transcription initiation
D	0000166	molecular_function	nucleotide binding
D	0001216	molecular_function	DNA-binding transcription activator activity
D	0001726	cellular_component	ruffle
D	0003677	molecular_function	DNA binding
D	0004518	molecular_function	nuclease activity
D	0004550	molecular_function	nucleoside diphosphate kinase activity
D	0004673	molecular_function	protein histidine kinase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005576	cellular_component	extracellular region
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005925	cellular_component	focal adhesion
D	0006183	biological_process	GTP biosynthetic process
D	0006228	biological_process	UTP biosynthetic process
D	0006241	biological_process	CTP biosynthetic process
D	0007155	biological_process	cell adhesion
D	0007229	biological_process	integrin-mediated signaling pathway
D	0009117	biological_process	nucleotide metabolic process
D	0009142	biological_process	nucleoside triphosphate biosynthetic process
D	0016301	molecular_function	kinase activity
D	0016740	molecular_function	transferase activity
D	0019003	molecular_function	GDP binding
D	0030027	cellular_component	lamellipodium
D	0034774	cellular_component	secretory granule lumen
D	0042304	biological_process	regulation of fatty acid biosynthetic process
D	0042802	molecular_function	identical protein binding
D	0042981	biological_process	regulation of apoptotic process
D	0043066	biological_process	negative regulation of apoptotic process
D	0045618	biological_process	positive regulation of keratinocyte differentiation
D	0045682	biological_process	regulation of epidermis development
D	0045893	biological_process	positive regulation of DNA-templated transcription
D	0045944	biological_process	positive regulation of transcription by RNA polymerase II
D	0046042	biological_process	ITP biosynthetic process
D	0046872	molecular_function	metal ion binding
D	0048471	cellular_component	perinuclear region of cytoplasm
D	0050679	biological_process	positive regulation of epithelial cell proliferation
D	0051880	molecular_function	G-quadruplex DNA binding
D	0070062	cellular_component	extracellular exosome
D	0071944	cellular_component	cell periphery
D	0120225	molecular_function	coenzyme A binding
D	1904813	cellular_component	ficolin-1-rich granule lumen
D	2000144	biological_process	positive regulation of DNA-templated transcription initiation
E	0000166	molecular_function	nucleotide binding
E	0001216	molecular_function	DNA-binding transcription activator activity
E	0001726	cellular_component	ruffle
E	0003677	molecular_function	DNA binding
E	0004518	molecular_function	nuclease activity
E	0004550	molecular_function	nucleoside diphosphate kinase activity
E	0004673	molecular_function	protein histidine kinase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005576	cellular_component	extracellular region
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005925	cellular_component	focal adhesion
E	0006183	biological_process	GTP biosynthetic process
E	0006228	biological_process	UTP biosynthetic process
E	0006241	biological_process	CTP biosynthetic process
E	0007155	biological_process	cell adhesion
E	0007229	biological_process	integrin-mediated signaling pathway
E	0009117	biological_process	nucleotide metabolic process
E	0009142	biological_process	nucleoside triphosphate biosynthetic process
E	0016301	molecular_function	kinase activity
E	0016740	molecular_function	transferase activity
E	0019003	molecular_function	GDP binding
E	0030027	cellular_component	lamellipodium
E	0034774	cellular_component	secretory granule lumen
E	0042304	biological_process	regulation of fatty acid biosynthetic process
E	0042802	molecular_function	identical protein binding
E	0042981	biological_process	regulation of apoptotic process
E	0043066	biological_process	negative regulation of apoptotic process
E	0045618	biological_process	positive regulation of keratinocyte differentiation
E	0045682	biological_process	regulation of epidermis development
E	0045893	biological_process	positive regulation of DNA-templated transcription
E	0045944	biological_process	positive regulation of transcription by RNA polymerase II
E	0046042	biological_process	ITP biosynthetic process
E	0046872	molecular_function	metal ion binding
E	0048471	cellular_component	perinuclear region of cytoplasm
E	0050679	biological_process	positive regulation of epithelial cell proliferation
E	0051880	molecular_function	G-quadruplex DNA binding
E	0070062	cellular_component	extracellular exosome
E	0071944	cellular_component	cell periphery
E	0120225	molecular_function	coenzyme A binding
E	1904813	cellular_component	ficolin-1-rich granule lumen
E	2000144	biological_process	positive regulation of DNA-templated transcription initiation
F	0000166	molecular_function	nucleotide binding
F	0001216	molecular_function	DNA-binding transcription activator activity
F	0001726	cellular_component	ruffle
F	0003677	molecular_function	DNA binding
F	0004518	molecular_function	nuclease activity
F	0004550	molecular_function	nucleoside diphosphate kinase activity
F	0004673	molecular_function	protein histidine kinase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005576	cellular_component	extracellular region
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005925	cellular_component	focal adhesion
F	0006183	biological_process	GTP biosynthetic process
F	0006228	biological_process	UTP biosynthetic process
F	0006241	biological_process	CTP biosynthetic process
F	0007155	biological_process	cell adhesion
F	0007229	biological_process	integrin-mediated signaling pathway
F	0009117	biological_process	nucleotide metabolic process
F	0009142	biological_process	nucleoside triphosphate biosynthetic process
F	0016301	molecular_function	kinase activity
F	0016740	molecular_function	transferase activity
F	0019003	molecular_function	GDP binding
F	0030027	cellular_component	lamellipodium
F	0034774	cellular_component	secretory granule lumen
F	0042304	biological_process	regulation of fatty acid biosynthetic process
F	0042802	molecular_function	identical protein binding
F	0042981	biological_process	regulation of apoptotic process
F	0043066	biological_process	negative regulation of apoptotic process
F	0045618	biological_process	positive regulation of keratinocyte differentiation
F	0045682	biological_process	regulation of epidermis development
F	0045893	biological_process	positive regulation of DNA-templated transcription
F	0045944	biological_process	positive regulation of transcription by RNA polymerase II
F	0046042	biological_process	ITP biosynthetic process
F	0046872	molecular_function	metal ion binding
F	0048471	cellular_component	perinuclear region of cytoplasm
F	0050679	biological_process	positive regulation of epithelial cell proliferation
F	0051880	molecular_function	G-quadruplex DNA binding
F	0070062	cellular_component	extracellular exosome
F	0071944	cellular_component	cell periphery
F	0120225	molecular_function	coenzyme A binding
F	1904813	cellular_component	ficolin-1-rich granule lumen
F	2000144	biological_process	positive regulation of DNA-templated transcription initiation

Functional Information from PROSITE/UniProt

site_id	PS00469
Number of Residues	9
Details	NDPK Nucleoside diphosphate kinase (NDPK) active site signature. NiiHGSDSV

Chain	Residue	Details
A	ASN119-VAL127

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	Active site: {"description":"Pros-phosphohistidine intermediate","evidences":[{"source":"PubMed","id":"1851158","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	36
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19435876","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3BBF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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