8PF8

Structure of Mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with Fragment-M-72

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003857	molecular_function	3-hydroxyacyl-CoA dehydrogenase activity
A	0004300	molecular_function	enoyl-CoA hydratase activity
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006631	biological_process	fatty acid metabolic process
A	0006635	biological_process	fatty acid beta-oxidation
A	0009274	cellular_component	peptidoglycan-based cell wall
A	0016042	biological_process	lipid catabolic process
A	0016509	molecular_function	long-chain-3-hydroxyacyl-CoA dehydrogenase activity
A	0016829	molecular_function	lyase activity
A	0044248	biological_process	cellular catabolic process
B	0000166	molecular_function	nucleotide binding
B	0003857	molecular_function	3-hydroxyacyl-CoA dehydrogenase activity
B	0004300	molecular_function	enoyl-CoA hydratase activity
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006631	biological_process	fatty acid metabolic process
B	0006635	biological_process	fatty acid beta-oxidation
B	0009274	cellular_component	peptidoglycan-based cell wall
B	0016042	biological_process	lipid catabolic process
B	0016509	molecular_function	long-chain-3-hydroxyacyl-CoA dehydrogenase activity
B	0016829	molecular_function	lyase activity
B	0044248	biological_process	cellular catabolic process
C	0005886	cellular_component	plasma membrane
C	0009274	cellular_component	peptidoglycan-based cell wall
C	0016746	molecular_function	acyltransferase activity
D	0005886	cellular_component	plasma membrane
D	0009274	cellular_component	peptidoglycan-based cell wall
D	0016746	molecular_function	acyltransferase activity

Functional Information from PROSITE/UniProt

site_id	PS00098
Number of Residues	19
Details	THIOLASE_1 Thiolases acyl-enzyme intermediate signature. LNRfCASGLeAVntaaqkV

Chain	Residue	Details
C	LEU88-VAL106

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site_id	PS00099
Number of Residues	14
Details	THIOLASE_3 Thiolases active site. ALITLCIGgGmGvA

Chain	Residue	Details
C	ALA384-ALA397

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site_id	PS00737
Number of Residues	17
Details	THIOLASE_2 Thiolases signature 2. NvnGGaIAmGHPlGaTG

Chain	Residue	Details
C	ASN349-GLY365

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Acyl-thioester intermediate => ECO:0000250

Chain	Residue	Details
C	CYS92
D	CYS92

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site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU10020

Chain	Residue	Details
C	HIS359
C	CYS389
D	HIS359
D	CYS389

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site_id	SWS_FT_FI3
Number of Residues	4
Details	CROSSLNK: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup) => ECO:0000269|PubMed:20066036

Chain	Residue	Details
C	LYS189
D	LYS189

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