8P5F

Human wild-type GAPDH,orthorhombic form

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
AAA	0000226	biological_process	microtubule cytoskeleton organization
AAA	0001819	biological_process	positive regulation of cytokine production
AAA	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
AAA	0005515	molecular_function	protein binding
AAA	0005634	cellular_component	nucleus
AAA	0005737	cellular_component	cytoplasm
AAA	0005811	cellular_component	lipid droplet
AAA	0005829	cellular_component	cytosol
AAA	0005856	cellular_component	cytoskeleton
AAA	0005886	cellular_component	plasma membrane
AAA	0006006	biological_process	glucose metabolic process
AAA	0006096	biological_process	glycolytic process
AAA	0006417	biological_process	regulation of translation
AAA	0006915	biological_process	apoptotic process
AAA	0008017	molecular_function	microtubule binding
AAA	0010951	biological_process	negative regulation of endopeptidase activity
AAA	0015630	cellular_component	microtubule cytoskeleton
AAA	0016020	cellular_component	membrane
AAA	0016241	biological_process	regulation of macroautophagy
AAA	0016491	molecular_function	oxidoreductase activity
AAA	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
AAA	0016740	molecular_function	transferase activity
AAA	0017148	biological_process	negative regulation of translation
AAA	0019828	molecular_function	aspartic-type endopeptidase inhibitor activity
AAA	0031640	biological_process	killing of cells of another organism
AAA	0031965	cellular_component	nuclear membrane
AAA	0031982	cellular_component	vesicle
AAA	0032481	biological_process	positive regulation of type I interferon production
AAA	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
AAA	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
AAA	0042802	molecular_function	identical protein binding
AAA	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
AAA	0043231	cellular_component	intracellular membrane-bounded organelle
AAA	0045087	biological_process	innate immune response
AAA	0048471	cellular_component	perinuclear region of cytoplasm
AAA	0050661	molecular_function	NADP binding
AAA	0050821	biological_process	protein stabilization
AAA	0050832	biological_process	defense response to fungus
AAA	0051287	molecular_function	NAD binding
AAA	0051402	biological_process	neuron apoptotic process
AAA	0051873	biological_process	killing by host of symbiont cells
AAA	0061844	biological_process	antimicrobial humoral immune response mediated by antimicrobial peptide
AAA	0070062	cellular_component	extracellular exosome
AAA	0071346	biological_process	cellular response to type II interferon
AAA	0097452	cellular_component	GAIT complex
AAA	0097718	molecular_function	disordered domain specific binding
AAA	1990904	cellular_component	ribonucleoprotein complex
DDD	0000226	biological_process	microtubule cytoskeleton organization
DDD	0001819	biological_process	positive regulation of cytokine production
DDD	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
DDD	0005515	molecular_function	protein binding
DDD	0005634	cellular_component	nucleus
DDD	0005737	cellular_component	cytoplasm
DDD	0005811	cellular_component	lipid droplet
DDD	0005829	cellular_component	cytosol
DDD	0005856	cellular_component	cytoskeleton
DDD	0005886	cellular_component	plasma membrane
DDD	0006006	biological_process	glucose metabolic process
DDD	0006096	biological_process	glycolytic process
DDD	0006417	biological_process	regulation of translation
DDD	0006915	biological_process	apoptotic process
DDD	0008017	molecular_function	microtubule binding
DDD	0010951	biological_process	negative regulation of endopeptidase activity
DDD	0015630	cellular_component	microtubule cytoskeleton
DDD	0016020	cellular_component	membrane
DDD	0016241	biological_process	regulation of macroautophagy
DDD	0016491	molecular_function	oxidoreductase activity
DDD	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
DDD	0016740	molecular_function	transferase activity
DDD	0017148	biological_process	negative regulation of translation
DDD	0019828	molecular_function	aspartic-type endopeptidase inhibitor activity
DDD	0031640	biological_process	killing of cells of another organism
DDD	0031965	cellular_component	nuclear membrane
DDD	0031982	cellular_component	vesicle
DDD	0032481	biological_process	positive regulation of type I interferon production
DDD	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
DDD	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
DDD	0042802	molecular_function	identical protein binding
DDD	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
DDD	0043231	cellular_component	intracellular membrane-bounded organelle
DDD	0045087	biological_process	innate immune response
DDD	0048471	cellular_component	perinuclear region of cytoplasm
DDD	0050661	molecular_function	NADP binding
DDD	0050821	biological_process	protein stabilization
DDD	0050832	biological_process	defense response to fungus
DDD	0051287	molecular_function	NAD binding
DDD	0051402	biological_process	neuron apoptotic process
DDD	0051873	biological_process	killing by host of symbiont cells
DDD	0061844	biological_process	antimicrobial humoral immune response mediated by antimicrobial peptide
DDD	0070062	cellular_component	extracellular exosome
DDD	0071346	biological_process	cellular response to type II interferon
DDD	0097452	cellular_component	GAIT complex
DDD	0097718	molecular_function	disordered domain specific binding
DDD	1990904	cellular_component	ribonucleoprotein complex
EEE	0000226	biological_process	microtubule cytoskeleton organization
EEE	0001819	biological_process	positive regulation of cytokine production
EEE	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
EEE	0005515	molecular_function	protein binding
EEE	0005634	cellular_component	nucleus
EEE	0005737	cellular_component	cytoplasm
EEE	0005811	cellular_component	lipid droplet
EEE	0005829	cellular_component	cytosol
EEE	0005856	cellular_component	cytoskeleton
EEE	0005886	cellular_component	plasma membrane
EEE	0006006	biological_process	glucose metabolic process
EEE	0006096	biological_process	glycolytic process
EEE	0006417	biological_process	regulation of translation
EEE	0006915	biological_process	apoptotic process
EEE	0008017	molecular_function	microtubule binding
EEE	0010951	biological_process	negative regulation of endopeptidase activity
EEE	0015630	cellular_component	microtubule cytoskeleton
EEE	0016020	cellular_component	membrane
EEE	0016241	biological_process	regulation of macroautophagy
EEE	0016491	molecular_function	oxidoreductase activity
EEE	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
EEE	0016740	molecular_function	transferase activity
EEE	0017148	biological_process	negative regulation of translation
EEE	0019828	molecular_function	aspartic-type endopeptidase inhibitor activity
EEE	0031640	biological_process	killing of cells of another organism
EEE	0031965	cellular_component	nuclear membrane
EEE	0031982	cellular_component	vesicle
EEE	0032481	biological_process	positive regulation of type I interferon production
EEE	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
EEE	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
EEE	0042802	molecular_function	identical protein binding
EEE	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
EEE	0043231	cellular_component	intracellular membrane-bounded organelle
EEE	0045087	biological_process	innate immune response
EEE	0048471	cellular_component	perinuclear region of cytoplasm
EEE	0050661	molecular_function	NADP binding
EEE	0050821	biological_process	protein stabilization
EEE	0050832	biological_process	defense response to fungus
EEE	0051287	molecular_function	NAD binding
EEE	0051402	biological_process	neuron apoptotic process
EEE	0051873	biological_process	killing by host of symbiont cells
EEE	0061844	biological_process	antimicrobial humoral immune response mediated by antimicrobial peptide
EEE	0070062	cellular_component	extracellular exosome
EEE	0071346	biological_process	cellular response to type II interferon
EEE	0097452	cellular_component	GAIT complex
EEE	0097718	molecular_function	disordered domain specific binding
EEE	1990904	cellular_component	ribonucleoprotein complex
GGG	0000226	biological_process	microtubule cytoskeleton organization
GGG	0001819	biological_process	positive regulation of cytokine production
GGG	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
GGG	0005515	molecular_function	protein binding
GGG	0005634	cellular_component	nucleus
GGG	0005737	cellular_component	cytoplasm
GGG	0005811	cellular_component	lipid droplet
GGG	0005829	cellular_component	cytosol
GGG	0005856	cellular_component	cytoskeleton
GGG	0005886	cellular_component	plasma membrane
GGG	0006006	biological_process	glucose metabolic process
GGG	0006096	biological_process	glycolytic process
GGG	0006417	biological_process	regulation of translation
GGG	0006915	biological_process	apoptotic process
GGG	0008017	molecular_function	microtubule binding
GGG	0010951	biological_process	negative regulation of endopeptidase activity
GGG	0015630	cellular_component	microtubule cytoskeleton
GGG	0016020	cellular_component	membrane
GGG	0016241	biological_process	regulation of macroautophagy
GGG	0016491	molecular_function	oxidoreductase activity
GGG	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
GGG	0016740	molecular_function	transferase activity
GGG	0017148	biological_process	negative regulation of translation
GGG	0019828	molecular_function	aspartic-type endopeptidase inhibitor activity
GGG	0031640	biological_process	killing of cells of another organism
GGG	0031965	cellular_component	nuclear membrane
GGG	0031982	cellular_component	vesicle
GGG	0032481	biological_process	positive regulation of type I interferon production
GGG	0035605	molecular_function	peptidyl-cysteine S-nitrosylase activity
GGG	0035606	biological_process	peptidyl-cysteine S-trans-nitrosylation
GGG	0042802	molecular_function	identical protein binding
GGG	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
GGG	0043231	cellular_component	intracellular membrane-bounded organelle
GGG	0045087	biological_process	innate immune response
GGG	0048471	cellular_component	perinuclear region of cytoplasm
GGG	0050661	molecular_function	NADP binding
GGG	0050821	biological_process	protein stabilization
GGG	0050832	biological_process	defense response to fungus
GGG	0051287	molecular_function	NAD binding
GGG	0051402	biological_process	neuron apoptotic process
GGG	0051873	biological_process	killing by host of symbiont cells
GGG	0061844	biological_process	antimicrobial humoral immune response mediated by antimicrobial peptide
GGG	0070062	cellular_component	extracellular exosome
GGG	0071346	biological_process	cellular response to type II interferon
GGG	0097452	cellular_component	GAIT complex
GGG	0097718	molecular_function	disordered domain specific binding
GGG	1990904	cellular_component	ribonucleoprotein complex

Functional Information from PROSITE/UniProt

site_id	PS00071
Number of Residues	8
Details	GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL

Chain	Residue	Details
AAA	ALA150-LEU157

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:25086035

Chain	Residue	Details
AAA	CSD152
DDD	CSD152
EEE	CSD152
GGG	CSD152

---

site_id	SWS_FT_FI2
Number of Residues	20
Details	BINDING: BINDING => ECO:0000269|PubMed:16239728, ECO:0000269|PubMed:16510976

Chain	Residue	Details
AAA	ARG13
AAA	ASP35
AAA	ARG80
AAA	SER122
AAA	ASN316
DDD	ARG13
DDD	ASP35
DDD	ARG80
DDD	SER122
DDD	ASN316
EEE	ARG13
EEE	ASP35
EEE	ARG80
EEE	SER122
EEE	ASN316
GGG	ARG13
GGG	ASP35
GGG	ARG80
GGG	SER122
GGG	ASN316

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P22513

Chain	Residue	Details
AAA	SER151
AAA	THR182
AAA	THR211
AAA	ARG234
DDD	SER151
DDD	THR182
DDD	THR211
DDD	ARG234
EEE	SER151
EEE	THR182
EEE	THR211
EEE	ARG234
GGG	SER151
GGG	THR182
GGG	THR211
GGG	ARG234

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	SITE: Activates thiol group during catalysis => ECO:0000305|PubMed:16239728, ECO:0000305|PubMed:16510976

Chain	Residue	Details
AAA	HIS179
DDD	HIS179
EEE	HIS179
GGG	HIS179

---

site_id	SWS_FT_FI5
Number of Residues	20
Details	MOD_RES: N6,N6-dimethyllysine => ECO:0000269|PubMed:18183946

Chain	Residue	Details
AAA	LYS5
AAA	LYS66
AAA	LYS260
AAA	LYS263
AAA	LYS334
DDD	LYS5
DDD	LYS66
DDD	LYS260
DDD	LYS263
DDD	LYS334
EEE	LYS5
EEE	LYS66
EEE	LYS260
EEE	LYS263
EEE	LYS334
GGG	LYS5
GGG	LYS66
GGG	LYS260
GGG	LYS263
GGG	LYS334

---

site_id	SWS_FT_FI6
Number of Residues	28
Details	MOD_RES: Deamidated asparagine => ECO:0000269|PubMed:18183946

Chain	Residue	Details
AAA	ASN9
AAA	ASN64
AAA	ASN70
AAA	ASN149
AAA	ASN155
AAA	ASN225
AAA	ASN316
DDD	ASN9
DDD	ASN64
DDD	ASN70
DDD	ASN149
DDD	ASN155
DDD	ASN225
DDD	ASN316
EEE	ASN9
EEE	ASN64
EEE	ASN70
EEE	ASN149
EEE	ASN155
EEE	ASN225
EEE	ASN316
GGG	ASN9
GGG	ASN64
GGG	ASN70
GGG	ASN149
GGG	ASN155
GGG	ASN225
GGG	ASN316

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:15592455

Chain	Residue	Details
AAA	TYR42
DDD	TYR42
EEE	TYR42
GGG	TYR42

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: Methionine sulfoxide; in vitro => ECO:0000305|PubMed:25086035

Chain	Residue	Details
AAA	MET46
DDD	MET46
EEE	MET46
GGG	MET46

---

site_id	SWS_FT_FI9
Number of Residues	12
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
AAA	LYS61
AAA	LYS219
AAA	LYS254
DDD	LYS61
DDD	LYS219
DDD	LYS254
EEE	LYS61
EEE	LYS219
EEE	LYS254
GGG	LYS61
GGG	LYS219
GGG	LYS254

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:18183946, ECO:0007744|PubMed:20068231

Chain	Residue	Details
AAA	THR75
DDD	THR75
EEE	THR75
GGG	THR75

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163

Chain	Residue	Details
AAA	SER83
DDD	SER83
EEE	SER83
GGG	SER83

---

site_id	SWS_FT_FI12
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:18183946

Chain	Residue	Details
AAA	SER122
AAA	SER148
DDD	SER122
DDD	SER148
EEE	SER122
EEE	SER148
GGG	SER122
GGG	SER148

---

site_id	SWS_FT_FI13
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163

Chain	Residue	Details
AAA	SER151
DDD	SER151
EEE	SER151
GGG	SER151

---

site_id	SWS_FT_FI14
Number of Residues	4
Details	MOD_RES: S-nitrosocysteine; in reversibly inhibited form => ECO:0000250|UniProtKB:P04797

Chain	Residue	Details
AAA	CSD152
DDD	CSD152
EEE	CSD152
GGG	CSD152

---

site_id	SWS_FT_FI15
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
AAA	THR153
DDD	THR153
EEE	THR153
GGG	THR153

---

site_id	SWS_FT_FI16
Number of Residues	8
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
AAA	THR177
AAA	THR182
DDD	THR177
DDD	THR182
EEE	THR177
EEE	THR182
GGG	THR177
GGG	THR182

---

site_id	SWS_FT_FI17
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:24275569

Chain	Residue	Details
AAA	THR184
DDD	THR184
EEE	THR184
GGG	THR184

---

site_id	SWS_FT_FI18
Number of Residues	8
Details	MOD_RES: N6-malonyllysine; alternate => ECO:0000269|PubMed:21908771

Chain	Residue	Details
AAA	LYS194
AAA	LYS215
DDD	LYS194
DDD	LYS215
EEE	LYS194
EEE	LYS215
GGG	LYS194
GGG	LYS215

---

site_id	SWS_FT_FI19
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:19690332

Chain	Residue	Details
AAA	THR211
DDD	THR211
EEE	THR211
GGG	THR211

---

site_id	SWS_FT_FI20
Number of Residues	4
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:19608861

Chain	Residue	Details
AAA	LYS227
DDD	LYS227
EEE	LYS227
GGG	LYS227

---

site_id	SWS_FT_FI21
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:18183946, ECO:0007744|PubMed:23186163

Chain	Residue	Details
AAA	THR229
DDD	THR229
EEE	THR229
GGG	THR229

---

site_id	SWS_FT_FI22
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:18183946

Chain	Residue	Details
AAA	THR237
DDD	THR237
EEE	THR237
GGG	THR237

---

site_id	SWS_FT_FI23
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
AAA	SER241
DDD	SER241
EEE	SER241
GGG	SER241

---

site_id	SWS_FT_FI24
Number of Residues	4
Details	MOD_RES: S-nitrosocysteine => ECO:0000269|PubMed:22771119, ECO:0000269|PubMed:25417112

Chain	Residue	Details
AAA	CYS247
DDD	CYS247
EEE	CYS247
GGG	CYS247

---

site_id	SWS_FT_FI25
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:18183946, ECO:0007744|PubMed:19690332

Chain	Residue	Details
AAA	SER312
DDD	SER312
EEE	SER312
GGG	SER312

---

site_id	SWS_FT_FI26
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
AAA	SER333
DDD	SER333
EEE	SER333
GGG	SER333

---

site_id	SWS_FT_FI27
Number of Residues	8
Details	CARBOHYD: (Microbial infection) N-beta-linked (GlcNAc) arginine => ECO:0000269|PubMed:28522607

Chain	Residue	Details
AAA	ARG197
AAA	ARG200
DDD	ARG197
DDD	ARG200
EEE	ARG197
EEE	ARG200
GGG	ARG197
GGG	ARG200

---

site_id	SWS_FT_FI28
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
AAA	LYS186
DDD	LYS186
EEE	LYS186
GGG	LYS186

---