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SummaryStructural detailsExperimental detailsFunctional detailsSequence NeighborHistoryDownloads

8P5F

Human wild-type GAPDH,orthorhombic form

This is a non-PDB format compatible entry.
Functional Information from GO Data
ChainGOidnamespacecontents
AAA0000226biological_processmicrotubule cytoskeleton organization
AAA0001819biological_processpositive regulation of cytokine production
AAA0002376biological_processimmune system process
AAA0004365molecular_functionglyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
AAA0005515molecular_functionprotein binding
AAA0005634cellular_componentnucleus
AAA0005737cellular_componentcytoplasm
AAA0005811cellular_componentlipid droplet
AAA0005829cellular_componentcytosol
AAA0005856cellular_componentcytoskeleton
AAA0005886cellular_componentplasma membrane
AAA0006006biological_processglucose metabolic process
AAA0006096biological_processglycolytic process
AAA0006417biological_processregulation of translation
AAA0006915biological_processapoptotic process
AAA0008017molecular_functionmicrotubule binding
AAA0010951biological_processnegative regulation of endopeptidase activity
AAA0015630cellular_componentmicrotubule cytoskeleton
AAA0016020cellular_componentmembrane
AAA0016241biological_processregulation of macroautophagy
AAA0016491molecular_functionoxidoreductase activity
AAA0016620molecular_functionoxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
AAA0016740molecular_functiontransferase activity
AAA0017148biological_processnegative regulation of translation
AAA0019828molecular_functionaspartic-type endopeptidase inhibitor activity
AAA0031640biological_processkilling of cells of another organism
AAA0031982cellular_componentvesicle
AAA0032481biological_processpositive regulation of type I interferon production
AAA0035605molecular_functionpeptidyl-cysteine S-nitrosylase activity
AAA0035606biological_processpeptidyl-cysteine S-trans-nitrosylation
AAA0042802molecular_functionidentical protein binding
AAA0043123biological_processpositive regulation of canonical NF-kappaB signal transduction
AAA0045087biological_processinnate immune response
AAA0048471cellular_componentperinuclear region of cytoplasm
AAA0050661molecular_functionNADP binding
AAA0050821biological_processprotein stabilization
AAA0050832biological_processdefense response to fungus
AAA0051287molecular_functionNAD binding
AAA0051402biological_processneuron apoptotic process
AAA0051873biological_processkilling by host of symbiont cells
AAA0061621biological_processcanonical glycolysis
AAA0061844biological_processantimicrobial humoral immune response mediated by antimicrobial peptide
AAA0070062cellular_componentextracellular exosome
AAA0071346biological_processcellular response to type II interferon
AAA0097452cellular_componentGAIT complex
AAA0097718molecular_functiondisordered domain specific binding
AAA1901194biological_processnegative regulation of formation of translation preinitiation complex
AAA1990904cellular_componentribonucleoprotein complex
DDD0000226biological_processmicrotubule cytoskeleton organization
DDD0001819biological_processpositive regulation of cytokine production
DDD0002376biological_processimmune system process
DDD0004365molecular_functionglyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
DDD0005515molecular_functionprotein binding
DDD0005634cellular_componentnucleus
DDD0005737cellular_componentcytoplasm
DDD0005811cellular_componentlipid droplet
DDD0005829cellular_componentcytosol
DDD0005856cellular_componentcytoskeleton
DDD0005886cellular_componentplasma membrane
DDD0006006biological_processglucose metabolic process
DDD0006096biological_processglycolytic process
DDD0006417biological_processregulation of translation
DDD0006915biological_processapoptotic process
DDD0008017molecular_functionmicrotubule binding
DDD0010951biological_processnegative regulation of endopeptidase activity
DDD0015630cellular_componentmicrotubule cytoskeleton
DDD0016020cellular_componentmembrane
DDD0016241biological_processregulation of macroautophagy
DDD0016491molecular_functionoxidoreductase activity
DDD0016620molecular_functionoxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
DDD0016740molecular_functiontransferase activity
DDD0017148biological_processnegative regulation of translation
DDD0019828molecular_functionaspartic-type endopeptidase inhibitor activity
DDD0031640biological_processkilling of cells of another organism
DDD0031982cellular_componentvesicle
DDD0032481biological_processpositive regulation of type I interferon production
DDD0035605molecular_functionpeptidyl-cysteine S-nitrosylase activity
DDD0035606biological_processpeptidyl-cysteine S-trans-nitrosylation
DDD0042802molecular_functionidentical protein binding
DDD0043123biological_processpositive regulation of canonical NF-kappaB signal transduction
DDD0045087biological_processinnate immune response
DDD0048471cellular_componentperinuclear region of cytoplasm
DDD0050661molecular_functionNADP binding
DDD0050821biological_processprotein stabilization
DDD0050832biological_processdefense response to fungus
DDD0051287molecular_functionNAD binding
DDD0051402biological_processneuron apoptotic process
DDD0051873biological_processkilling by host of symbiont cells
DDD0061621biological_processcanonical glycolysis
DDD0061844biological_processantimicrobial humoral immune response mediated by antimicrobial peptide
DDD0070062cellular_componentextracellular exosome
DDD0071346biological_processcellular response to type II interferon
DDD0097452cellular_componentGAIT complex
DDD0097718molecular_functiondisordered domain specific binding
DDD1901194biological_processnegative regulation of formation of translation preinitiation complex
DDD1990904cellular_componentribonucleoprotein complex
EEE0000226biological_processmicrotubule cytoskeleton organization
EEE0001819biological_processpositive regulation of cytokine production
EEE0002376biological_processimmune system process
EEE0004365molecular_functionglyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
EEE0005515molecular_functionprotein binding
EEE0005634cellular_componentnucleus
EEE0005737cellular_componentcytoplasm
EEE0005811cellular_componentlipid droplet
EEE0005829cellular_componentcytosol
EEE0005856cellular_componentcytoskeleton
EEE0005886cellular_componentplasma membrane
EEE0006006biological_processglucose metabolic process
EEE0006096biological_processglycolytic process
EEE0006417biological_processregulation of translation
EEE0006915biological_processapoptotic process
EEE0008017molecular_functionmicrotubule binding
EEE0010951biological_processnegative regulation of endopeptidase activity
EEE0015630cellular_componentmicrotubule cytoskeleton
EEE0016020cellular_componentmembrane
EEE0016241biological_processregulation of macroautophagy
EEE0016491molecular_functionoxidoreductase activity
EEE0016620molecular_functionoxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
EEE0016740molecular_functiontransferase activity
EEE0017148biological_processnegative regulation of translation
EEE0019828molecular_functionaspartic-type endopeptidase inhibitor activity
EEE0031640biological_processkilling of cells of another organism
EEE0031982cellular_componentvesicle
EEE0032481biological_processpositive regulation of type I interferon production
EEE0035605molecular_functionpeptidyl-cysteine S-nitrosylase activity
EEE0035606biological_processpeptidyl-cysteine S-trans-nitrosylation
EEE0042802molecular_functionidentical protein binding
EEE0043123biological_processpositive regulation of canonical NF-kappaB signal transduction
EEE0045087biological_processinnate immune response
EEE0048471cellular_componentperinuclear region of cytoplasm
EEE0050661molecular_functionNADP binding
EEE0050821biological_processprotein stabilization
EEE0050832biological_processdefense response to fungus
EEE0051287molecular_functionNAD binding
EEE0051402biological_processneuron apoptotic process
EEE0051873biological_processkilling by host of symbiont cells
EEE0061621biological_processcanonical glycolysis
EEE0061844biological_processantimicrobial humoral immune response mediated by antimicrobial peptide
EEE0070062cellular_componentextracellular exosome
EEE0071346biological_processcellular response to type II interferon
EEE0097452cellular_componentGAIT complex
EEE0097718molecular_functiondisordered domain specific binding
EEE1901194biological_processnegative regulation of formation of translation preinitiation complex
EEE1990904cellular_componentribonucleoprotein complex
GGG0000226biological_processmicrotubule cytoskeleton organization
GGG0001819biological_processpositive regulation of cytokine production
GGG0002376biological_processimmune system process
GGG0004365molecular_functionglyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
GGG0005515molecular_functionprotein binding
GGG0005634cellular_componentnucleus
GGG0005737cellular_componentcytoplasm
GGG0005811cellular_componentlipid droplet
GGG0005829cellular_componentcytosol
GGG0005856cellular_componentcytoskeleton
GGG0005886cellular_componentplasma membrane
GGG0006006biological_processglucose metabolic process
GGG0006096biological_processglycolytic process
GGG0006417biological_processregulation of translation
GGG0006915biological_processapoptotic process
GGG0008017molecular_functionmicrotubule binding
GGG0010951biological_processnegative regulation of endopeptidase activity
GGG0015630cellular_componentmicrotubule cytoskeleton
GGG0016020cellular_componentmembrane
GGG0016241biological_processregulation of macroautophagy
GGG0016491molecular_functionoxidoreductase activity
GGG0016620molecular_functionoxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
GGG0016740molecular_functiontransferase activity
GGG0017148biological_processnegative regulation of translation
GGG0019828molecular_functionaspartic-type endopeptidase inhibitor activity
GGG0031640biological_processkilling of cells of another organism
GGG0031982cellular_componentvesicle
GGG0032481biological_processpositive regulation of type I interferon production
GGG0035605molecular_functionpeptidyl-cysteine S-nitrosylase activity
GGG0035606biological_processpeptidyl-cysteine S-trans-nitrosylation
GGG0042802molecular_functionidentical protein binding
GGG0043123biological_processpositive regulation of canonical NF-kappaB signal transduction
GGG0045087biological_processinnate immune response
GGG0048471cellular_componentperinuclear region of cytoplasm
GGG0050661molecular_functionNADP binding
GGG0050821biological_processprotein stabilization
GGG0050832biological_processdefense response to fungus
GGG0051287molecular_functionNAD binding
GGG0051402biological_processneuron apoptotic process
GGG0051873biological_processkilling by host of symbiont cells
GGG0061621biological_processcanonical glycolysis
GGG0061844biological_processantimicrobial humoral immune response mediated by antimicrobial peptide
GGG0070062cellular_componentextracellular exosome
GGG0071346biological_processcellular response to type II interferon
GGG0097452cellular_componentGAIT complex
GGG0097718molecular_functiondisordered domain specific binding
GGG1901194biological_processnegative regulation of formation of translation preinitiation complex
GGG1990904cellular_componentribonucleoprotein complex
Functional Information from PROSITE/UniProt
site_idPS00071
Number of Residues8
DetailsGAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL
ChainResidueDetails
AAAALA150-LEU157
Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues146
DetailsRegion: {"description":"Interaction with WARS1","evidences":[{"source":"PubMed","id":"15628863","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI2
Number of Residues20
DetailsMotif: {"description":"[IL]-x-C-x-x-[DE] motif","evidences":[{"source":"PubMed","id":"25417112","evidenceCode":"ECO:0000305"}]}
ChainResidueDetails
site_idSWS_FT_FI3
Number of Residues4
DetailsActive site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"25086035","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI4
Number of Residues20
DetailsBinding site: {"evidences":[{"source":"PubMed","id":"16239728","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16510976","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI5
Number of Residues12
DetailsBinding site: {"evidences":[{"source":"UniProtKB","id":"P22513","evidenceCode":"ECO:0000250"}]}
ChainResidueDetails
site_idSWS_FT_FI6
Number of Residues4
DetailsSite: {"description":"Activates thiol group during catalysis","evidences":[{"source":"PubMed","id":"16239728","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"16510976","evidenceCode":"ECO:0000305"}]}
ChainResidueDetails
site_idSWS_FT_FI7
Number of Residues16
DetailsModified residue: {"description":"N6,N6-dimethyllysine","evidences":[{"source":"PubMed","id":"18183946","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI8
Number of Residues28
DetailsModified residue: {"description":"Deamidated asparagine","evidences":[{"source":"PubMed","id":"18183946","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI9
Number of Residues4
DetailsModified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"15592455","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI10
Number of Residues4
DetailsModified residue: {"description":"Methionine sulfoxide; in vitro","evidences":[{"source":"PubMed","id":"25086035","evidenceCode":"ECO:0000305"}]}
ChainResidueDetails
site_idSWS_FT_FI11
Number of Residues12
DetailsModified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI12
Number of Residues4
DetailsModified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"18183946","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI13
Number of Residues4
DetailsModified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"17081983","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"21406692","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI14
Number of Residues8
DetailsModified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18183946","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI15
Number of Residues4
DetailsModified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI16
Number of Residues4
DetailsModified residue: {"description":"S-nitrosocysteine; in reversibly inhibited form","evidences":[{"source":"UniProtKB","id":"P04797","evidenceCode":"ECO:0000250"}]}
ChainResidueDetails
site_idSWS_FT_FI17
Number of Residues4
DetailsModified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI18
Number of Residues8
DetailsModified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI19
Number of Residues4
DetailsModified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI20
Number of Residues8
DetailsModified residue: {"description":"N6-malonyllysine; alternate","evidences":[{"source":"PubMed","id":"21908771","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI21
Number of Residues4
DetailsModified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI22
Number of Residues4
DetailsModified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI23
Number of Residues4
DetailsModified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"18183946","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI24
Number of Residues4
DetailsModified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"18183946","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI25
Number of Residues4
DetailsModified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI26
Number of Residues4
DetailsModified residue: {"description":"S-nitrosocysteine","evidences":[{"source":"PubMed","id":"22771119","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25417112","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI27
Number of Residues4
DetailsModified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18183946","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI28
Number of Residues8
DetailsGlycosylation: {"description":"(Microbial infection) N-beta-linked (GlcNAc) arginine","evidences":[{"source":"PubMed","id":"28522607","evidenceCode":"ECO:0000269"}]}
ChainResidueDetails
site_idSWS_FT_FI29
Number of Residues8
DetailsCross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails

246031

PDB entries from 2025-12-10

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