8P4O

CryoEM structure of a GroEL7-GroES7 cage with encapsulated ordered substrate MetK in the presence of ADP-BeFx

Functional Information from GO Data

Chain	GOid	namespace	contents
1	0000166	molecular_function	nucleotide binding
1	0000287	molecular_function	magnesium ion binding
1	0004478	molecular_function	methionine adenosyltransferase activity
1	0005515	molecular_function	protein binding
1	0005524	molecular_function	ATP binding
1	0005737	cellular_component	cytoplasm
1	0005829	cellular_component	cytosol
1	0006556	biological_process	S-adenosylmethionine biosynthetic process
1	0006730	biological_process	one-carbon metabolic process
1	0016740	molecular_function	transferase activity
1	0030955	molecular_function	potassium ion binding
1	0033353	biological_process	S-adenosylmethionine cycle
1	0042802	molecular_function	identical protein binding
1	0046872	molecular_function	metal ion binding
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006457	biological_process	protein folding
A	0009314	biological_process	response to radiation
A	0009408	biological_process	response to heat
A	0016020	cellular_component	membrane
A	0016853	molecular_function	isomerase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0019068	biological_process	virion assembly
A	0042026	biological_process	protein refolding
A	0042802	molecular_function	identical protein binding
A	0051082	molecular_function	unfolded protein binding
A	0140662	molecular_function	ATP-dependent protein folding chaperone
A	1990220	cellular_component	GroEL-GroES complex
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006457	biological_process	protein folding
B	0009314	biological_process	response to radiation
B	0009408	biological_process	response to heat
B	0016020	cellular_component	membrane
B	0016853	molecular_function	isomerase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0019068	biological_process	virion assembly
B	0042026	biological_process	protein refolding
B	0042802	molecular_function	identical protein binding
B	0051082	molecular_function	unfolded protein binding
B	0140662	molecular_function	ATP-dependent protein folding chaperone
B	1990220	cellular_component	GroEL-GroES complex
C	0000166	molecular_function	nucleotide binding
C	0000287	molecular_function	magnesium ion binding
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006457	biological_process	protein folding
C	0009314	biological_process	response to radiation
C	0009408	biological_process	response to heat
C	0016020	cellular_component	membrane
C	0016853	molecular_function	isomerase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0019068	biological_process	virion assembly
C	0042026	biological_process	protein refolding
C	0042802	molecular_function	identical protein binding
C	0051082	molecular_function	unfolded protein binding
C	0140662	molecular_function	ATP-dependent protein folding chaperone
C	1990220	cellular_component	GroEL-GroES complex
D	0000166	molecular_function	nucleotide binding
D	0000287	molecular_function	magnesium ion binding
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006457	biological_process	protein folding
D	0009314	biological_process	response to radiation
D	0009408	biological_process	response to heat
D	0016020	cellular_component	membrane
D	0016853	molecular_function	isomerase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0019068	biological_process	virion assembly
D	0042026	biological_process	protein refolding
D	0042802	molecular_function	identical protein binding
D	0051082	molecular_function	unfolded protein binding
D	0140662	molecular_function	ATP-dependent protein folding chaperone
D	1990220	cellular_component	GroEL-GroES complex
E	0000166	molecular_function	nucleotide binding
E	0000287	molecular_function	magnesium ion binding
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006457	biological_process	protein folding
E	0009314	biological_process	response to radiation
E	0009408	biological_process	response to heat
E	0016020	cellular_component	membrane
E	0016853	molecular_function	isomerase activity
E	0016887	molecular_function	ATP hydrolysis activity
E	0019068	biological_process	virion assembly
E	0042026	biological_process	protein refolding
E	0042802	molecular_function	identical protein binding
E	0051082	molecular_function	unfolded protein binding
E	0140662	molecular_function	ATP-dependent protein folding chaperone
E	1990220	cellular_component	GroEL-GroES complex
F	0000166	molecular_function	nucleotide binding
F	0000287	molecular_function	magnesium ion binding
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006457	biological_process	protein folding
F	0009314	biological_process	response to radiation
F	0009408	biological_process	response to heat
F	0016020	cellular_component	membrane
F	0016853	molecular_function	isomerase activity
F	0016887	molecular_function	ATP hydrolysis activity
F	0019068	biological_process	virion assembly
F	0042026	biological_process	protein refolding
F	0042802	molecular_function	identical protein binding
F	0051082	molecular_function	unfolded protein binding
F	0140662	molecular_function	ATP-dependent protein folding chaperone
F	1990220	cellular_component	GroEL-GroES complex
G	0000166	molecular_function	nucleotide binding
G	0000287	molecular_function	magnesium ion binding
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006457	biological_process	protein folding
G	0009314	biological_process	response to radiation
G	0009408	biological_process	response to heat
G	0016020	cellular_component	membrane
G	0016853	molecular_function	isomerase activity
G	0016887	molecular_function	ATP hydrolysis activity
G	0019068	biological_process	virion assembly
G	0042026	biological_process	protein refolding
G	0042802	molecular_function	identical protein binding
G	0051082	molecular_function	unfolded protein binding
G	0140662	molecular_function	ATP-dependent protein folding chaperone
G	1990220	cellular_component	GroEL-GroES complex
O	0005515	molecular_function	protein binding
O	0005524	molecular_function	ATP binding
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0006457	biological_process	protein folding
O	0009408	biological_process	response to heat
O	0019068	biological_process	virion assembly
O	0042802	molecular_function	identical protein binding
O	0044183	molecular_function	protein folding chaperone
O	0046872	molecular_function	metal ion binding
O	0051082	molecular_function	unfolded protein binding
O	0051087	molecular_function	protein-folding chaperone binding
O	1990220	cellular_component	GroEL-GroES complex
P	0005515	molecular_function	protein binding
P	0005524	molecular_function	ATP binding
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0006457	biological_process	protein folding
P	0009408	biological_process	response to heat
P	0019068	biological_process	virion assembly
P	0042802	molecular_function	identical protein binding
P	0044183	molecular_function	protein folding chaperone
P	0046872	molecular_function	metal ion binding
P	0051082	molecular_function	unfolded protein binding
P	0051087	molecular_function	protein-folding chaperone binding
P	1990220	cellular_component	GroEL-GroES complex
Q	0005515	molecular_function	protein binding
Q	0005524	molecular_function	ATP binding
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0006457	biological_process	protein folding
Q	0009408	biological_process	response to heat
Q	0019068	biological_process	virion assembly
Q	0042802	molecular_function	identical protein binding
Q	0044183	molecular_function	protein folding chaperone
Q	0046872	molecular_function	metal ion binding
Q	0051082	molecular_function	unfolded protein binding
Q	0051087	molecular_function	protein-folding chaperone binding
Q	1990220	cellular_component	GroEL-GroES complex
R	0005515	molecular_function	protein binding
R	0005524	molecular_function	ATP binding
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0006457	biological_process	protein folding
R	0009408	biological_process	response to heat
R	0019068	biological_process	virion assembly
R	0042802	molecular_function	identical protein binding
R	0044183	molecular_function	protein folding chaperone
R	0046872	molecular_function	metal ion binding
R	0051082	molecular_function	unfolded protein binding
R	0051087	molecular_function	protein-folding chaperone binding
R	1990220	cellular_component	GroEL-GroES complex
S	0005515	molecular_function	protein binding
S	0005524	molecular_function	ATP binding
S	0005737	cellular_component	cytoplasm
S	0005829	cellular_component	cytosol
S	0006457	biological_process	protein folding
S	0009408	biological_process	response to heat
S	0019068	biological_process	virion assembly
S	0042802	molecular_function	identical protein binding
S	0044183	molecular_function	protein folding chaperone
S	0046872	molecular_function	metal ion binding
S	0051082	molecular_function	unfolded protein binding
S	0051087	molecular_function	protein-folding chaperone binding
S	1990220	cellular_component	GroEL-GroES complex
T	0005515	molecular_function	protein binding
T	0005524	molecular_function	ATP binding
T	0005737	cellular_component	cytoplasm
T	0005829	cellular_component	cytosol
T	0006457	biological_process	protein folding
T	0009408	biological_process	response to heat
T	0019068	biological_process	virion assembly
T	0042802	molecular_function	identical protein binding
T	0044183	molecular_function	protein folding chaperone
T	0046872	molecular_function	metal ion binding
T	0051082	molecular_function	unfolded protein binding
T	0051087	molecular_function	protein-folding chaperone binding
T	1990220	cellular_component	GroEL-GroES complex
U	0005515	molecular_function	protein binding
U	0005524	molecular_function	ATP binding
U	0005737	cellular_component	cytoplasm
U	0005829	cellular_component	cytosol
U	0006457	biological_process	protein folding
U	0009408	biological_process	response to heat
U	0019068	biological_process	virion assembly
U	0042802	molecular_function	identical protein binding
U	0044183	molecular_function	protein folding chaperone
U	0046872	molecular_function	metal ion binding
U	0051082	molecular_function	unfolded protein binding
U	0051087	molecular_function	protein-folding chaperone binding
U	1990220	cellular_component	GroEL-GroES complex

Functional Information from PROSITE/UniProt

site_id	PS00681
Number of Residues	25
Details	CHAPERONINS_CPN10 Chaperonins cpn10 signature. IrPLhDRVIVKrkevetksaGgIVL

Chain	Residue	Details
O	ILE3-LEU27

---

site_id	PS00296
Number of Residues	12
Details	CHAPERONINS_CPN60 Chaperonins cpn60 signature. AAVEEGVVaGGG

Chain	Residue	Details
A	ALA405-GLY416

---

site_id	PS00376
Number of Residues	11
Details	ADOMET_SYNTHASE_1 S-adenosylmethionine synthase signature 1. GAGDQGlmfGY

Chain	Residue	Details
1	GLY116-TYR126

---

site_id	PS00377
Number of Residues	9
Details	ADOMET_SYNTHASE_2 S-adenosylmethionine synthase signature 2. GGGAFSgKD

Chain	Residue	Details
1	GLY259-ASP267

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	77
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00600","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"25174333","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"14517228","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"9285585","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1AON","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1PCQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1PF9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3WVL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00600","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"25174333","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"14517228","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"9285585","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1AON","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1PF9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3WVL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	42
Details	Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"PubMed","id":"21151122","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	7
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"PubMed","id":"21151122","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	10
Details	Region: {"description":"Flexible loop","evidences":[{"source":"HAMAP-Rule","id":"MF_00086","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"14967023","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	Binding site: {"description":"in other chain","evidences":[{"source":"HAMAP-Rule","id":"MF_00086","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"14967023","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1P7L","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1RG9","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00086","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"14967023","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1P7L","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1RG9","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00086","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"14967023","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7629147","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1MXC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1P7L","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1RG9","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00086","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"14967023","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1P7L","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---