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8P41

Crystal structure of glucocerebrosidase in complex with allosteric activator

Functional Information from GO Data
ChainGOidnamespacecontents
A0000423biological_processmitophagy
A0004336molecular_functiongalactosylceramidase activity
A0004348molecular_functionglucosylceramidase activity
A0005102molecular_functionsignaling receptor binding
A0005124molecular_functionscavenger receptor binding
A0005515molecular_functionprotein binding
A0005615cellular_componentextracellular space
A0005764cellular_componentlysosome
A0005765cellular_componentlysosomal membrane
A0005783cellular_componentendoplasmic reticulum
A0005794cellular_componentGolgi apparatus
A0005802cellular_componenttrans-Golgi network
A0006665biological_processsphingolipid metabolic process
A0006678biological_processglucosylceramide metabolic process
A0006680biological_processglucosylceramide catabolic process
A0006914biological_processautophagy
A0007005biological_processmitochondrion organization
A0007040biological_processlysosome organization
A0007417biological_processcentral nervous system development
A0008203biological_processcholesterol metabolic process
A0008340biological_processdetermination of adult lifespan
A0008422molecular_functionbeta-glucosidase activity
A0009267biological_processcellular response to starvation
A0009268biological_processresponse to pH
A0014004biological_processmicroglia differentiation
A0016020cellular_componentmembrane
A0016241biological_processregulation of macroautophagy
A0016757molecular_functionglycosyltransferase activity
A0016787molecular_functionhydrolase activity
A0016798molecular_functionhydrolase activity, acting on glycosyl bonds
A0019882biological_processantigen processing and presentation
A0019915biological_processlipid storage
A0021694biological_processcerebellar Purkinje cell layer formation
A0021859biological_processpyramidal neuron differentiation
A0022904biological_processrespiratory electron transport chain
A0023021biological_processtermination of signal transduction
A0030259biological_processlipid glycosylation
A0031175biological_processneuron projection development
A0031333biological_processnegative regulation of protein-containing complex assembly
A0032006biological_processregulation of TOR signaling
A0032436biological_processpositive regulation of proteasomal ubiquitin-dependent protein catabolic process
A0032715biological_processnegative regulation of interleukin-6 production
A0033077biological_processT cell differentiation in thymus
A0033574biological_processresponse to testosterone
A0042391biological_processregulation of membrane potential
A0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
A0043202cellular_componentlysosomal lumen
A0043243biological_processpositive regulation of protein-containing complex disassembly
A0043409biological_processnegative regulation of MAPK cascade
A0043524biological_processnegative regulation of neuron apoptotic process
A0043627biological_processresponse to estrogen
A0046512biological_processsphingosine biosynthetic process
A0046513biological_processceramide biosynthetic process
A0046527molecular_functionglucosyltransferase activity
A0048469biological_processcell maturation
A0048854biological_processbrain morphogenesis
A0048872biological_processhomeostasis of number of cells
A0050295molecular_functionsteryl-beta-glucosidase activity
A0050728biological_processnegative regulation of inflammatory response
A0050877biological_processnervous system process
A0050905biological_processneuromuscular process
A0051246biological_processregulation of protein metabolic process
A0051248biological_processnegative regulation of protein metabolic process
A0051402biological_processneuron apoptotic process
A0061436biological_processestablishment of skin barrier
A0061518biological_processmicroglial cell proliferation
A0061744biological_processmotor behavior
A0070062cellular_componentextracellular exosome
A0071356biological_processcellular response to tumor necrosis factor
A0071425biological_processhematopoietic stem cell proliferation
A0071548biological_processresponse to dexamethasone
A0072676biological_processlymphocyte migration
A0097066biological_processresponse to thyroid hormone
A0098773biological_processskin epidermis development
A1901805biological_processbeta-glucoside catabolic process
A1903052biological_processpositive regulation of proteolysis involved in protein catabolic process
A1903061biological_processpositive regulation of protein lipidation
A1904457biological_processpositive regulation of neuronal action potential
A1905037biological_processautophagosome organization
A1905091biological_processpositive regulation of type 2 mitophagy
A1905165biological_processregulation of lysosomal protein catabolic process
B0000423biological_processmitophagy
B0004336molecular_functiongalactosylceramidase activity
B0004348molecular_functionglucosylceramidase activity
B0005102molecular_functionsignaling receptor binding
B0005124molecular_functionscavenger receptor binding
B0005515molecular_functionprotein binding
B0005615cellular_componentextracellular space
B0005764cellular_componentlysosome
B0005765cellular_componentlysosomal membrane
B0005783cellular_componentendoplasmic reticulum
B0005794cellular_componentGolgi apparatus
B0005802cellular_componenttrans-Golgi network
B0006665biological_processsphingolipid metabolic process
B0006678biological_processglucosylceramide metabolic process
B0006680biological_processglucosylceramide catabolic process
B0006914biological_processautophagy
B0007005biological_processmitochondrion organization
B0007040biological_processlysosome organization
B0007417biological_processcentral nervous system development
B0008203biological_processcholesterol metabolic process
B0008340biological_processdetermination of adult lifespan
B0008422molecular_functionbeta-glucosidase activity
B0009267biological_processcellular response to starvation
B0009268biological_processresponse to pH
B0014004biological_processmicroglia differentiation
B0016020cellular_componentmembrane
B0016241biological_processregulation of macroautophagy
B0016757molecular_functionglycosyltransferase activity
B0016787molecular_functionhydrolase activity
B0016798molecular_functionhydrolase activity, acting on glycosyl bonds
B0019882biological_processantigen processing and presentation
B0019915biological_processlipid storage
B0021694biological_processcerebellar Purkinje cell layer formation
B0021859biological_processpyramidal neuron differentiation
B0022904biological_processrespiratory electron transport chain
B0023021biological_processtermination of signal transduction
B0030259biological_processlipid glycosylation
B0031175biological_processneuron projection development
B0031333biological_processnegative regulation of protein-containing complex assembly
B0032006biological_processregulation of TOR signaling
B0032436biological_processpositive regulation of proteasomal ubiquitin-dependent protein catabolic process
B0032715biological_processnegative regulation of interleukin-6 production
B0033077biological_processT cell differentiation in thymus
B0033574biological_processresponse to testosterone
B0042391biological_processregulation of membrane potential
B0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
B0043202cellular_componentlysosomal lumen
B0043243biological_processpositive regulation of protein-containing complex disassembly
B0043409biological_processnegative regulation of MAPK cascade
B0043524biological_processnegative regulation of neuron apoptotic process
B0043627biological_processresponse to estrogen
B0046512biological_processsphingosine biosynthetic process
B0046513biological_processceramide biosynthetic process
B0046527molecular_functionglucosyltransferase activity
B0048469biological_processcell maturation
B0048854biological_processbrain morphogenesis
B0048872biological_processhomeostasis of number of cells
B0050295molecular_functionsteryl-beta-glucosidase activity
B0050728biological_processnegative regulation of inflammatory response
B0050877biological_processnervous system process
B0050905biological_processneuromuscular process
B0051246biological_processregulation of protein metabolic process
B0051248biological_processnegative regulation of protein metabolic process
B0051402biological_processneuron apoptotic process
B0061436biological_processestablishment of skin barrier
B0061518biological_processmicroglial cell proliferation
B0061744biological_processmotor behavior
B0070062cellular_componentextracellular exosome
B0071356biological_processcellular response to tumor necrosis factor
B0071425biological_processhematopoietic stem cell proliferation
B0071548biological_processresponse to dexamethasone
B0072676biological_processlymphocyte migration
B0097066biological_processresponse to thyroid hormone
B0098773biological_processskin epidermis development
B1901805biological_processbeta-glucoside catabolic process
B1903052biological_processpositive regulation of proteolysis involved in protein catabolic process
B1903061biological_processpositive regulation of protein lipidation
B1904457biological_processpositive regulation of neuronal action potential
B1905037biological_processautophagosome organization
B1905091biological_processpositive regulation of type 2 mitophagy
B1905165biological_processregulation of lysosomal protein catabolic process
Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues2
DetailsACT_SITE: Proton donor => ECO:0000269|PubMed:15817452
ChainResidueDetails
AGLU235
BGLU235

site_idSWS_FT_FI2
Number of Residues2
DetailsACT_SITE: Nucleophile => ECO:0000269|PubMed:15817452
ChainResidueDetails
AGLU340
BGLU340

site_idSWS_FT_FI3
Number of Residues2
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:12792654, ECO:0000269|PubMed:17139081
ChainResidueDetails
AASN19
BASN19

site_idSWS_FT_FI4
Number of Residues2
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:12754519, ECO:0000269|PubMed:17139081, ECO:0000269|PubMed:19159218
ChainResidueDetails
AASN59
BASN59

site_idSWS_FT_FI5
Number of Residues2
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:12754519, ECO:0000269|PubMed:17139081
ChainResidueDetails
AASN146
BASN146

site_idSWS_FT_FI6
Number of Residues2
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:12754519, ECO:0000269|PubMed:19159218
ChainResidueDetails
AASN270
BASN270

site_idSWS_FT_FI7
Number of Residues2
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255
ChainResidueDetails
AASN462
BASN462

Catalytic Information from CSA
site_idMCSA1
Number of Residues4
DetailsM-CSA 764
ChainResidueDetails
AGLU235activator, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor
AGLU340covalently attached, nucleofuge, nucleophile
ACYS342electrostatic stabiliser
AASN370

site_idMCSA2
Number of Residues4
DetailsM-CSA 764
ChainResidueDetails
BGLU235activator, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor
BGLU340covalently attached, nucleofuge, nucleophile
BCYS342electrostatic stabiliser
BASN370

227344

PDB entries from 2024-11-13

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