8P0J

Cryo EM map and model of the vaccinia RNA polymerase intermediate pre-initiation open promoter complex

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000428	cellular_component	DNA-directed RNA polymerase complex
A	0003677	molecular_function	DNA binding
A	0003899	molecular_function	DNA-directed RNA polymerase activity
A	0006351	biological_process	DNA-templated transcription
A	0016740	molecular_function	transferase activity
A	0016779	molecular_function	nucleotidyltransferase activity
A	0034062	molecular_function	5'-3' RNA polymerase activity
A	0044423	cellular_component	virion component
B	0000428	cellular_component	DNA-directed RNA polymerase complex
B	0003677	molecular_function	DNA binding
B	0003899	molecular_function	DNA-directed RNA polymerase activity
B	0006351	biological_process	DNA-templated transcription
B	0016740	molecular_function	transferase activity
B	0016779	molecular_function	nucleotidyltransferase activity
B	0032549	molecular_function	ribonucleoside binding
B	0034062	molecular_function	5'-3' RNA polymerase activity
B	0044423	cellular_component	virion component
B	0046872	molecular_function	metal ion binding
C	0000428	cellular_component	DNA-directed RNA polymerase complex
C	0003677	molecular_function	DNA binding
C	0003899	molecular_function	DNA-directed RNA polymerase activity
C	0016740	molecular_function	transferase activity
C	0016779	molecular_function	nucleotidyltransferase activity
C	0019083	biological_process	viral transcription
C	0034062	molecular_function	5'-3' RNA polymerase activity
C	0044423	cellular_component	virion component
E	0000428	cellular_component	DNA-directed RNA polymerase complex
E	0003677	molecular_function	DNA binding
E	0003899	molecular_function	DNA-directed RNA polymerase activity
E	0016740	molecular_function	transferase activity
E	0016779	molecular_function	nucleotidyltransferase activity
E	0019083	biological_process	viral transcription
E	0034062	molecular_function	5'-3' RNA polymerase activity
E	0044423	cellular_component	virion component
F	0000428	cellular_component	DNA-directed RNA polymerase complex
F	0003677	molecular_function	DNA binding
F	0003899	molecular_function	DNA-directed RNA polymerase activity
F	0006351	biological_process	DNA-templated transcription
F	0016740	molecular_function	transferase activity
F	0016779	molecular_function	nucleotidyltransferase activity
F	0034062	molecular_function	5'-3' RNA polymerase activity
F	0044423	cellular_component	virion component
G	0000428	cellular_component	DNA-directed RNA polymerase complex
G	0003677	molecular_function	DNA binding
G	0003899	molecular_function	DNA-directed RNA polymerase activity
G	0016740	molecular_function	transferase activity
G	0016779	molecular_function	nucleotidyltransferase activity
G	0019083	biological_process	viral transcription
G	0034062	molecular_function	5'-3' RNA polymerase activity
G	0044423	cellular_component	virion component
H	0006351	biological_process	DNA-templated transcription
I	0006351	biological_process	DNA-templated transcription
J	0000428	cellular_component	DNA-directed RNA polymerase complex
J	0003677	molecular_function	DNA binding
J	0003899	molecular_function	DNA-directed RNA polymerase activity
J	0006351	biological_process	DNA-templated transcription
J	0016740	molecular_function	transferase activity
J	0016779	molecular_function	nucleotidyltransferase activity
J	0034062	molecular_function	5'-3' RNA polymerase activity
J	0044423	cellular_component	virion component
L	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
L	0006351	biological_process	DNA-templated transcription
L	0006353	biological_process	DNA-templated transcription termination
L	0006370	biological_process	7-methylguanosine mRNA capping
L	0006397	biological_process	mRNA processing
L	0044423	cellular_component	virion component
L	0106005	biological_process	RNA 5'-cap (guanine-N7)-methylation
O	0000166	molecular_function	nucleotide binding
O	0003723	molecular_function	RNA binding
O	0003824	molecular_function	catalytic activity
O	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
O	0004484	molecular_function	mRNA guanylyltransferase activity
O	0004651	molecular_function	polynucleotide 5'-phosphatase activity
O	0005525	molecular_function	GTP binding
O	0006370	biological_process	7-methylguanosine mRNA capping
O	0006397	biological_process	mRNA processing
O	0008168	molecular_function	methyltransferase activity
O	0016740	molecular_function	transferase activity
O	0016779	molecular_function	nucleotidyltransferase activity
O	0016787	molecular_function	hydrolase activity
O	0032259	biological_process	methylation
O	0044423	cellular_component	virion component
O	0046872	molecular_function	metal ion binding
O	0050355	molecular_function	inorganic triphosphate phosphatase activity
O	0106005	biological_process	RNA 5'-cap (guanine-N7)-methylation
O	0140818	molecular_function	mRNA 5'-triphosphate monophosphatase activity
S	0000428	cellular_component	DNA-directed RNA polymerase complex
S	0003676	molecular_function	nucleic acid binding
S	0003677	molecular_function	DNA binding
S	0003899	molecular_function	DNA-directed RNA polymerase activity
S	0006351	biological_process	DNA-templated transcription
S	0008270	molecular_function	zinc ion binding
S	0016740	molecular_function	transferase activity
S	0016779	molecular_function	nucleotidyltransferase activity
S	0030430	cellular_component	host cell cytoplasm
S	0034062	molecular_function	5'-3' RNA polymerase activity
S	0044423	cellular_component	virion component
S	0046872	molecular_function	metal ion binding

Functional Information from PROSITE/UniProt

site_id	PS00466
Number of Residues	36
Details	ZF_TFIIS_1 Zinc finger TFIIS-type signature. CpnCksrnttpmmIQTRAaDEPplvrhaCrd...CkqhF

Chain	Residue	Details
S	CYS159-PHE194

---

site_id	PS01166
Number of Residues	13
Details	RNA_POL_BETA RNA polymerases beta chain signature. GdKFTTrTSQKGT

Chain	Residue	Details
B	GLY880-THR892

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	328
Details	Domain: {"description":"mRNA cap 0 methyltransferase","evidences":[{"source":"PROSITE-ProRule","id":"PRU00895","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	538
Details	Region: {"description":"Triphosphatase-guanylyltransferase","evidences":[{"source":"UniProtKB","id":"P04298","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	Active site: {"description":"N6-GMP-lysine intermediate","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P04298","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00895","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	5
Details	Site: {"description":"Essential for RNA triphosphatase activity","evidences":[{"source":"UniProtKB","id":"P04298","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	5
Details	Site: {"description":"mRNA cap binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00895","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---