8OF8
Cryo-EM structure of actomyosin-5a-S1 with the full-length lever (nucleotide free)
Functional Information from PROSITE/UniProt
| site_id | PS00018 |
| Number of Residues | 13 |
| Details | EF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL |
| Chain | Residue | Details |
| A | ASP20-LEU32 | |
| A | ASP56-PHE68 | |
| A | ASP93-LEU105 | |
| A | ASP129-PHE141 |
| site_id | PS00406 |
| Number of Residues | 11 |
| Details | ACTINS_1 Actins signature 1. YVGDEAQs.KRG |
| Chain | Residue | Details |
| J | TYR53-GLY63 |
| site_id | PS00432 |
| Number of Residues | 9 |
| Details | ACTINS_2 Actins signature 2. WITKqEYDE |
| Chain | Residue | Details |
| J | TRP356-GLU364 |
| site_id | PS01132 |
| Number of Residues | 13 |
| Details | ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR |
| Chain | Residue | Details |
| J | LEU104-ARG116 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000255 |
| Chain | Residue | Details |
| M | GLY163 | |
| A | GLU67 | |
| E | GLU140 | |
| F | ASP20 | |
| F | ASP22 | |
| F | ASP24 | |
| F | THR26 | |
| F | GLU31 | |
| F | ASP56 | |
| F | ASP58 | |
| F | ASN60 | |
| F | THR62 | |
| A | ASP93 | |
| F | GLU67 | |
| F | ASP93 | |
| F | ASP95 | |
| F | ASN97 | |
| F | TYR99 | |
| F | GLU104 | |
| F | ASP129 | |
| F | ASP131 | |
| F | ASP133 | |
| F | GLN135 | |
| A | ASP95 | |
| F | GLU140 | |
| A | ASN97 | |
| A | TYR99 | |
| A | GLU104 | |
| A | ASP129 | |
| A | ASP131 | |
| A | ASP133 | |
| A | GLN135 | |
| K | ASP1 | |
| A | GLU140 | |
| B | ASP20 | |
| B | ASP22 | |
| B | ASP24 | |
| B | THR26 | |
| B | GLU31 | |
| B | ASP56 | |
| B | ASP58 | |
| B | ASN60 | |
| B | THR62 | |
| L | ASP1 | |
| B | GLU67 | |
| B | ASP93 | |
| B | ASP95 | |
| B | ASN97 | |
| B | TYR99 | |
| B | GLU104 | |
| B | ASP129 | |
| B | ASP131 | |
| B | ASP133 | |
| B | GLN135 | |
| A | THR26 | |
| B | GLU140 | |
| C | ASP20 | |
| C | ASP22 | |
| C | ASP24 | |
| C | THR26 | |
| C | GLU31 | |
| C | ASP56 | |
| C | ASP58 | |
| C | ASN60 | |
| C | THR62 | |
| A | GLU31 | |
| C | GLU67 | |
| C | ASP93 | |
| C | ASP95 | |
| C | ASN97 | |
| C | TYR99 | |
| C | GLU104 | |
| C | ASP129 | |
| C | ASP131 | |
| C | ASP133 | |
| C | GLN135 | |
| A | ASP56 | |
| C | GLU140 | |
| D | ASP20 | |
| D | ASP22 | |
| D | ASP24 | |
| D | THR26 | |
| D | GLU31 | |
| D | ASP56 | |
| D | ASP58 | |
| D | ASN60 | |
| D | THR62 | |
| A | ASP58 | |
| D | GLU67 | |
| D | ASP93 | |
| D | ASP95 | |
| D | ASN97 | |
| D | TYR99 | |
| D | GLU104 | |
| D | ASP129 | |
| D | ASP131 | |
| D | ASP133 | |
| D | GLN135 | |
| A | ASN60 | |
| D | GLU140 | |
| E | ASP20 | |
| E | ASP22 | |
| E | ASP24 | |
| E | THR26 | |
| E | GLU31 | |
| E | ASP56 | |
| E | ASP58 | |
| E | ASN60 | |
| E | THR62 | |
| A | THR62 | |
| E | GLU67 | |
| E | ASP93 | |
| E | ASP95 | |
| E | ASN97 | |
| E | TYR99 | |
| E | GLU104 | |
| E | ASP129 | |
| E | ASP131 | |
| E | ASP133 | |
| E | GLN135 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | MOD_RES: N-acetylalanine => ECO:0000250|UniProtKB:Q9Y4I1 |
| Chain | Residue | Details |
| M | ALA2 | |
| J | MET47 | |
| K | MET44 | |
| K | MET47 | |
| L | MET44 | |
| L | MET47 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:19144319, ECO:0007744|PubMed:21183079 |
| Chain | Residue | Details |
| M | SER600 | |
| K | LYS61 | |
| L | LYS61 | |
| D | LYS21 | |
| E | LYS21 | |
| F | LYS21 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 3 |
| Details | MOD_RES: Tele-methylhistidine => ECO:0000269|PubMed:1150665, ECO:0000269|PubMed:16905096, ECO:0000269|PubMed:213279, ECO:0000269|PubMed:2395459, ECO:0000269|PubMed:499690, ECO:0007744|PDB:1ATN, ECO:0007744|PDB:1NWK |
| Chain | Residue | Details |
| J | HIC73 | |
| K | HIC73 | |
| L | HIC73 | |
| D | THR44 | |
| E | THR44 | |
| F | THR44 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 3 |
| Details | MOD_RES: N6-methyllysine => ECO:0000250|UniProtKB:P68133 |
| Chain | Residue | Details |
| J | LYS84 | |
| K | LYS84 | |
| L | LYS84 | |
| D | SER81 | |
| E | SER81 | |
| F | SER81 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 3 |
| Details | MOD_RES: ADP-ribosylarginine; by SpvB => ECO:0000305|PubMed:16905096 |
| Chain | Residue | Details |
| J | ARG177 | |
| K | ARG177 | |
| L | ARG177 | |
| D | LYS94 | |
| E | LYS94 | |
| F | LYS94 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 6 |
| Details | MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18034455, ECO:0007744|PubMed:21183079 |
| Chain | Residue | Details |
| A | TYR99 | |
| B | TYR99 | |
| C | TYR99 | |
| D | TYR99 | |
| E | TYR99 | |
| F | TYR99 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 6 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:21183079 |
| Chain | Residue | Details |
| A | SER101 | |
| B | SER101 | |
| C | SER101 | |
| D | SER101 | |
| E | SER101 | |
| F | SER101 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 6 |
| Details | MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P0DP23 |
| Chain | Residue | Details |
| A | THR110 | |
| B | THR110 | |
| C | THR110 | |
| D | THR110 | |
| E | THR110 | |
| F | THR110 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 6 |
| Details | MOD_RES: N6-methyllysine; alternate => ECO:0000250|UniProtKB:P0DP23 |
| Chain | Residue | Details |
| A | LYS115 | |
| B | LYS115 | |
| C | LYS115 | |
| D | LYS115 | |
| E | LYS115 | |
| F | LYS115 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 6 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P0DP23 |
| Chain | Residue | Details |
| A | TYR138 | |
| B | TYR138 | |
| C | TYR138 | |
| D | TYR138 | |
| E | TYR138 | |
| F | TYR138 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 12 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P62157 |
| Chain | Residue | Details |
| A | LYS21 | |
| F | LYS21 | |
| B | LYS21 | |
| C | LYS21 | |
| D | LYS21 | |
| E | LYS21 |
