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8OF8

Cryo-EM structure of actomyosin-5a-S1 with the full-length lever (nucleotide free)

Functional Information from GO Data
ChainGOidnamespacecontents
A0000086biological_processG2/M transition of mitotic cell cycle
A0000922cellular_componentspindle pole
A0002027biological_processregulation of heart rate
A0005509molecular_functioncalcium ion binding
A0005513biological_processdetection of calcium ion
A0005515molecular_functionprotein binding
A0005654cellular_componentnucleoplasm
A0005737cellular_componentcytoplasm
A0005813cellular_componentcentrosome
A0005819cellular_componentspindle
A0005829cellular_componentcytosol
A0005856cellular_componentcytoskeleton
A0005876cellular_componentspindle microtubule
A0008076cellular_componentvoltage-gated potassium channel complex
A0008179molecular_functionadenylate cyclase binding
A0010856molecular_functionadenylate cyclase activator activity
A0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
A0016240biological_processautophagosome membrane docking
A0019855molecular_functioncalcium channel inhibitor activity
A0019901molecular_functionprotein kinase binding
A0030017cellular_componentsarcomere
A0031432molecular_functiontitin binding
A0031514cellular_componentmotile cilium
A0032465biological_processregulation of cytokinesis
A0032991cellular_componentprotein-containing complex
A0034704cellular_componentcalcium channel complex
A0035458biological_processcellular response to interferon-beta
A0043209cellular_componentmyelin sheath
A0043539molecular_functionprotein serine/threonine kinase activator activity
A0044305cellular_componentcalyx of Held
A0044325molecular_functiontransmembrane transporter binding
A0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
A0046872molecular_functionmetal ion binding
A0051592biological_processresponse to calcium ion
A0051649biological_processestablishment of localization in cell
A0055117biological_processregulation of cardiac muscle contraction
A0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
A0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
A0071346biological_processcellular response to type II interferon
A0072542molecular_functionprotein phosphatase activator activity
A0097225cellular_componentsperm midpiece
A0097720biological_processcalcineurin-mediated signaling
A0098901biological_processregulation of cardiac muscle cell action potential
A0140056biological_processorganelle localization by membrane tethering
A0140238biological_processpresynaptic endocytosis
A0150034cellular_componentdistal axon
A1902494cellular_componentcatalytic complex
A1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
B0000086biological_processG2/M transition of mitotic cell cycle
B0000922cellular_componentspindle pole
B0002027biological_processregulation of heart rate
B0005509molecular_functioncalcium ion binding
B0005513biological_processdetection of calcium ion
B0005515molecular_functionprotein binding
B0005654cellular_componentnucleoplasm
B0005737cellular_componentcytoplasm
B0005813cellular_componentcentrosome
B0005819cellular_componentspindle
B0005829cellular_componentcytosol
B0005856cellular_componentcytoskeleton
B0005876cellular_componentspindle microtubule
B0008076cellular_componentvoltage-gated potassium channel complex
B0008179molecular_functionadenylate cyclase binding
B0010856molecular_functionadenylate cyclase activator activity
B0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
B0016240biological_processautophagosome membrane docking
B0019855molecular_functioncalcium channel inhibitor activity
B0019901molecular_functionprotein kinase binding
B0030017cellular_componentsarcomere
B0031432molecular_functiontitin binding
B0031514cellular_componentmotile cilium
B0032465biological_processregulation of cytokinesis
B0032991cellular_componentprotein-containing complex
B0034704cellular_componentcalcium channel complex
B0035458biological_processcellular response to interferon-beta
B0043209cellular_componentmyelin sheath
B0043539molecular_functionprotein serine/threonine kinase activator activity
B0044305cellular_componentcalyx of Held
B0044325molecular_functiontransmembrane transporter binding
B0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
B0046872molecular_functionmetal ion binding
B0051592biological_processresponse to calcium ion
B0051649biological_processestablishment of localization in cell
B0055117biological_processregulation of cardiac muscle contraction
B0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
B0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
B0071346biological_processcellular response to type II interferon
B0072542molecular_functionprotein phosphatase activator activity
B0097225cellular_componentsperm midpiece
B0097720biological_processcalcineurin-mediated signaling
B0098901biological_processregulation of cardiac muscle cell action potential
B0140056biological_processorganelle localization by membrane tethering
B0140238biological_processpresynaptic endocytosis
B0150034cellular_componentdistal axon
B1902494cellular_componentcatalytic complex
B1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
C0000086biological_processG2/M transition of mitotic cell cycle
C0000922cellular_componentspindle pole
C0002027biological_processregulation of heart rate
C0005509molecular_functioncalcium ion binding
C0005513biological_processdetection of calcium ion
C0005515molecular_functionprotein binding
C0005654cellular_componentnucleoplasm
C0005737cellular_componentcytoplasm
C0005813cellular_componentcentrosome
C0005819cellular_componentspindle
C0005829cellular_componentcytosol
C0005856cellular_componentcytoskeleton
C0005876cellular_componentspindle microtubule
C0008076cellular_componentvoltage-gated potassium channel complex
C0008179molecular_functionadenylate cyclase binding
C0010856molecular_functionadenylate cyclase activator activity
C0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
C0016240biological_processautophagosome membrane docking
C0019855molecular_functioncalcium channel inhibitor activity
C0019901molecular_functionprotein kinase binding
C0030017cellular_componentsarcomere
C0031432molecular_functiontitin binding
C0031514cellular_componentmotile cilium
C0032465biological_processregulation of cytokinesis
C0032991cellular_componentprotein-containing complex
C0034704cellular_componentcalcium channel complex
C0035458biological_processcellular response to interferon-beta
C0043209cellular_componentmyelin sheath
C0043539molecular_functionprotein serine/threonine kinase activator activity
C0044305cellular_componentcalyx of Held
C0044325molecular_functiontransmembrane transporter binding
C0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
C0046872molecular_functionmetal ion binding
C0051592biological_processresponse to calcium ion
C0051649biological_processestablishment of localization in cell
C0055117biological_processregulation of cardiac muscle contraction
C0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
C0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
C0071346biological_processcellular response to type II interferon
C0072542molecular_functionprotein phosphatase activator activity
C0097225cellular_componentsperm midpiece
C0097720biological_processcalcineurin-mediated signaling
C0098901biological_processregulation of cardiac muscle cell action potential
C0140056biological_processorganelle localization by membrane tethering
C0140238biological_processpresynaptic endocytosis
C0150034cellular_componentdistal axon
C1902494cellular_componentcatalytic complex
C1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
D0000086biological_processG2/M transition of mitotic cell cycle
D0000922cellular_componentspindle pole
D0002027biological_processregulation of heart rate
D0005509molecular_functioncalcium ion binding
D0005513biological_processdetection of calcium ion
D0005515molecular_functionprotein binding
D0005654cellular_componentnucleoplasm
D0005737cellular_componentcytoplasm
D0005813cellular_componentcentrosome
D0005819cellular_componentspindle
D0005829cellular_componentcytosol
D0005856cellular_componentcytoskeleton
D0005876cellular_componentspindle microtubule
D0008076cellular_componentvoltage-gated potassium channel complex
D0008179molecular_functionadenylate cyclase binding
D0010856molecular_functionadenylate cyclase activator activity
D0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
D0016240biological_processautophagosome membrane docking
D0019855molecular_functioncalcium channel inhibitor activity
D0019901molecular_functionprotein kinase binding
D0030017cellular_componentsarcomere
D0031432molecular_functiontitin binding
D0031514cellular_componentmotile cilium
D0032465biological_processregulation of cytokinesis
D0032991cellular_componentprotein-containing complex
D0034704cellular_componentcalcium channel complex
D0035458biological_processcellular response to interferon-beta
D0043209cellular_componentmyelin sheath
D0043539molecular_functionprotein serine/threonine kinase activator activity
D0044305cellular_componentcalyx of Held
D0044325molecular_functiontransmembrane transporter binding
D0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
D0046872molecular_functionmetal ion binding
D0051592biological_processresponse to calcium ion
D0051649biological_processestablishment of localization in cell
D0055117biological_processregulation of cardiac muscle contraction
D0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
D0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
D0071346biological_processcellular response to type II interferon
D0072542molecular_functionprotein phosphatase activator activity
D0097225cellular_componentsperm midpiece
D0097720biological_processcalcineurin-mediated signaling
D0098901biological_processregulation of cardiac muscle cell action potential
D0140056biological_processorganelle localization by membrane tethering
D0140238biological_processpresynaptic endocytosis
D0150034cellular_componentdistal axon
D1902494cellular_componentcatalytic complex
D1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
E0000086biological_processG2/M transition of mitotic cell cycle
E0000922cellular_componentspindle pole
E0002027biological_processregulation of heart rate
E0005509molecular_functioncalcium ion binding
E0005513biological_processdetection of calcium ion
E0005515molecular_functionprotein binding
E0005654cellular_componentnucleoplasm
E0005737cellular_componentcytoplasm
E0005813cellular_componentcentrosome
E0005819cellular_componentspindle
E0005829cellular_componentcytosol
E0005856cellular_componentcytoskeleton
E0005876cellular_componentspindle microtubule
E0008076cellular_componentvoltage-gated potassium channel complex
E0008179molecular_functionadenylate cyclase binding
E0010856molecular_functionadenylate cyclase activator activity
E0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
E0016240biological_processautophagosome membrane docking
E0019855molecular_functioncalcium channel inhibitor activity
E0019901molecular_functionprotein kinase binding
E0030017cellular_componentsarcomere
E0031432molecular_functiontitin binding
E0031514cellular_componentmotile cilium
E0032465biological_processregulation of cytokinesis
E0032991cellular_componentprotein-containing complex
E0034704cellular_componentcalcium channel complex
E0035458biological_processcellular response to interferon-beta
E0043209cellular_componentmyelin sheath
E0043539molecular_functionprotein serine/threonine kinase activator activity
E0044305cellular_componentcalyx of Held
E0044325molecular_functiontransmembrane transporter binding
E0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
E0046872molecular_functionmetal ion binding
E0051592biological_processresponse to calcium ion
E0051649biological_processestablishment of localization in cell
E0055117biological_processregulation of cardiac muscle contraction
E0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
E0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
E0071346biological_processcellular response to type II interferon
E0072542molecular_functionprotein phosphatase activator activity
E0097225cellular_componentsperm midpiece
E0097720biological_processcalcineurin-mediated signaling
E0098901biological_processregulation of cardiac muscle cell action potential
E0140056biological_processorganelle localization by membrane tethering
E0140238biological_processpresynaptic endocytosis
E0150034cellular_componentdistal axon
E1902494cellular_componentcatalytic complex
E1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
F0000086biological_processG2/M transition of mitotic cell cycle
F0000922cellular_componentspindle pole
F0002027biological_processregulation of heart rate
F0005509molecular_functioncalcium ion binding
F0005513biological_processdetection of calcium ion
F0005515molecular_functionprotein binding
F0005654cellular_componentnucleoplasm
F0005737cellular_componentcytoplasm
F0005813cellular_componentcentrosome
F0005819cellular_componentspindle
F0005829cellular_componentcytosol
F0005856cellular_componentcytoskeleton
F0005876cellular_componentspindle microtubule
F0008076cellular_componentvoltage-gated potassium channel complex
F0008179molecular_functionadenylate cyclase binding
F0010856molecular_functionadenylate cyclase activator activity
F0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
F0016240biological_processautophagosome membrane docking
F0019855molecular_functioncalcium channel inhibitor activity
F0019901molecular_functionprotein kinase binding
F0030017cellular_componentsarcomere
F0031432molecular_functiontitin binding
F0031514cellular_componentmotile cilium
F0032465biological_processregulation of cytokinesis
F0032991cellular_componentprotein-containing complex
F0034704cellular_componentcalcium channel complex
F0035458biological_processcellular response to interferon-beta
F0043209cellular_componentmyelin sheath
F0043539molecular_functionprotein serine/threonine kinase activator activity
F0044305cellular_componentcalyx of Held
F0044325molecular_functiontransmembrane transporter binding
F0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
F0046872molecular_functionmetal ion binding
F0051592biological_processresponse to calcium ion
F0051649biological_processestablishment of localization in cell
F0055117biological_processregulation of cardiac muscle contraction
F0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
F0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
F0071346biological_processcellular response to type II interferon
F0072542molecular_functionprotein phosphatase activator activity
F0097225cellular_componentsperm midpiece
F0097720biological_processcalcineurin-mediated signaling
F0098901biological_processregulation of cardiac muscle cell action potential
F0140056biological_processorganelle localization by membrane tethering
F0140238biological_processpresynaptic endocytosis
F0150034cellular_componentdistal axon
F1902494cellular_componentcatalytic complex
F1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
J0000287molecular_functionmagnesium ion binding
J0001725cellular_componentstress fiber
J0003785molecular_functionactin monomer binding
J0005509molecular_functioncalcium ion binding
J0005515molecular_functionprotein binding
J0005523molecular_functiontropomyosin binding
J0005524molecular_functionATP binding
J0005737cellular_componentcytoplasm
J0005856cellular_componentcytoskeleton
J0005865cellular_componentstriated muscle thin filament
J0005884cellular_componentactin filament
J0010628biological_processpositive regulation of gene expression
J0016787molecular_functionhydrolase activity
J0019904molecular_functionprotein domain specific binding
J0030027cellular_componentlamellipodium
J0030041biological_processactin filament polymerization
J0030175cellular_componentfilopodium
J0030240biological_processskeletal muscle thin filament assembly
J0031013molecular_functiontroponin I binding
J0031432molecular_functiontitin binding
J0031941cellular_componentfilamentous actin
J0032036molecular_functionmyosin heavy chain binding
J0032432cellular_componentactin filament bundle
J0042802molecular_functionidentical protein binding
J0044297cellular_componentcell body
J0048306molecular_functioncalcium-dependent protein binding
J0048741biological_processskeletal muscle fiber development
J0051017biological_processactin filament bundle assembly
J0090131biological_processmesenchyme migration
J0098723cellular_componentskeletal muscle myofibril
J0140660molecular_functioncytoskeletal motor activator activity
K0000287molecular_functionmagnesium ion binding
K0001725cellular_componentstress fiber
K0003785molecular_functionactin monomer binding
K0005509molecular_functioncalcium ion binding
K0005515molecular_functionprotein binding
K0005523molecular_functiontropomyosin binding
K0005524molecular_functionATP binding
K0005737cellular_componentcytoplasm
K0005856cellular_componentcytoskeleton
K0005865cellular_componentstriated muscle thin filament
K0005884cellular_componentactin filament
K0010628biological_processpositive regulation of gene expression
K0016787molecular_functionhydrolase activity
K0019904molecular_functionprotein domain specific binding
K0030027cellular_componentlamellipodium
K0030041biological_processactin filament polymerization
K0030175cellular_componentfilopodium
K0030240biological_processskeletal muscle thin filament assembly
K0031013molecular_functiontroponin I binding
K0031432molecular_functiontitin binding
K0031941cellular_componentfilamentous actin
K0032036molecular_functionmyosin heavy chain binding
K0032432cellular_componentactin filament bundle
K0042802molecular_functionidentical protein binding
K0044297cellular_componentcell body
K0048306molecular_functioncalcium-dependent protein binding
K0048741biological_processskeletal muscle fiber development
K0051017biological_processactin filament bundle assembly
K0090131biological_processmesenchyme migration
K0098723cellular_componentskeletal muscle myofibril
K0140660molecular_functioncytoskeletal motor activator activity
L0000287molecular_functionmagnesium ion binding
L0001725cellular_componentstress fiber
L0003785molecular_functionactin monomer binding
L0005509molecular_functioncalcium ion binding
L0005515molecular_functionprotein binding
L0005523molecular_functiontropomyosin binding
L0005524molecular_functionATP binding
L0005737cellular_componentcytoplasm
L0005856cellular_componentcytoskeleton
L0005865cellular_componentstriated muscle thin filament
L0005884cellular_componentactin filament
L0010628biological_processpositive regulation of gene expression
L0016787molecular_functionhydrolase activity
L0019904molecular_functionprotein domain specific binding
L0030027cellular_componentlamellipodium
L0030041biological_processactin filament polymerization
L0030175cellular_componentfilopodium
L0030240biological_processskeletal muscle thin filament assembly
L0031013molecular_functiontroponin I binding
L0031432molecular_functiontitin binding
L0031941cellular_componentfilamentous actin
L0032036molecular_functionmyosin heavy chain binding
L0032432cellular_componentactin filament bundle
L0042802molecular_functionidentical protein binding
L0044297cellular_componentcell body
L0048306molecular_functioncalcium-dependent protein binding
L0048741biological_processskeletal muscle fiber development
L0051017biological_processactin filament bundle assembly
L0090131biological_processmesenchyme migration
L0098723cellular_componentskeletal muscle myofibril
L0140660molecular_functioncytoskeletal motor activator activity
Functional Information from PROSITE/UniProt
site_idPS00018
Number of Residues13
DetailsEF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL
ChainResidueDetails
AASP20-LEU32
AASP56-PHE68
AASP93-LEU105
AASP129-PHE141

site_idPS00406
Number of Residues11
DetailsACTINS_1 Actins signature 1. YVGDEAQs.KRG
ChainResidueDetails
JTYR53-GLY63

site_idPS00432
Number of Residues9
DetailsACTINS_2 Actins signature 2. WITKqEYDE
ChainResidueDetails
JTRP356-GLU364

site_idPS01132
Number of Residues13
DetailsACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR
ChainResidueDetails
JLEU104-ARG116

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues1
DetailsBINDING: BINDING => ECO:0000255
ChainResidueDetails
MGLY163
AGLU67
EGLU140
FASP20
FASP22
FASP24
FTHR26
FGLU31
FASP56
FASP58
FASN60
FTHR62
AASP93
FGLU67
FASP93
FASP95
FASN97
FTYR99
FGLU104
FASP129
FASP131
FASP133
FGLN135
AASP95
FGLU140
AASN97
ATYR99
AGLU104
AASP129
AASP131
AASP133
AGLN135
KASP1
AGLU140
BASP20
BASP22
BASP24
BTHR26
BGLU31
BASP56
BASP58
BASN60
BTHR62
LASP1
BGLU67
BASP93
BASP95
BASN97
BTYR99
BGLU104
BASP129
BASP131
BASP133
BGLN135
ATHR26
BGLU140
CASP20
CASP22
CASP24
CTHR26
CGLU31
CASP56
CASP58
CASN60
CTHR62
AGLU31
CGLU67
CASP93
CASP95
CASN97
CTYR99
CGLU104
CASP129
CASP131
CASP133
CGLN135
AASP56
CGLU140
DASP20
DASP22
DASP24
DTHR26
DGLU31
DASP56
DASP58
DASN60
DTHR62
AASP58
DGLU67
DASP93
DASP95
DASN97
DTYR99
DGLU104
DASP129
DASP131
DASP133
DGLN135
AASN60
DGLU140
EASP20
EASP22
EASP24
ETHR26
EGLU31
EASP56
EASP58
EASN60
ETHR62
ATHR62
EGLU67
EASP93
EASP95
EASN97
ETYR99
EGLU104
EASP129
EASP131
EASP133
EGLN135

site_idSWS_FT_FI2
Number of Residues1
DetailsMOD_RES: N-acetylalanine => ECO:0000250|UniProtKB:Q9Y4I1
ChainResidueDetails
MALA2
JMET47
KMET44
KMET47
LMET44
LMET47

site_idSWS_FT_FI3
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:19144319, ECO:0007744|PubMed:21183079
ChainResidueDetails
MSER600
KLYS61
LLYS61
DLYS21
ELYS21
FLYS21

site_idSWS_FT_FI4
Number of Residues3
DetailsMOD_RES: Tele-methylhistidine => ECO:0000269|PubMed:1150665, ECO:0000269|PubMed:16905096, ECO:0000269|PubMed:213279, ECO:0000269|PubMed:2395459, ECO:0000269|PubMed:499690, ECO:0007744|PDB:1ATN, ECO:0007744|PDB:1NWK
ChainResidueDetails
JHIC73
KHIC73
LHIC73
DTHR44
ETHR44
FTHR44

site_idSWS_FT_FI5
Number of Residues3
DetailsMOD_RES: N6-methyllysine => ECO:0000250|UniProtKB:P68133
ChainResidueDetails
JLYS84
KLYS84
LLYS84
DSER81
ESER81
FSER81

site_idSWS_FT_FI6
Number of Residues3
DetailsMOD_RES: ADP-ribosylarginine; by SpvB => ECO:0000305|PubMed:16905096
ChainResidueDetails
JARG177
KARG177
LARG177
DLYS94
ELYS94
FLYS94

site_idSWS_FT_FI7
Number of Residues6
DetailsMOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18034455, ECO:0007744|PubMed:21183079
ChainResidueDetails
ATYR99
BTYR99
CTYR99
DTYR99
ETYR99
FTYR99

site_idSWS_FT_FI8
Number of Residues6
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:21183079
ChainResidueDetails
ASER101
BSER101
CSER101
DSER101
ESER101
FSER101

site_idSWS_FT_FI9
Number of Residues6
DetailsMOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
ATHR110
BTHR110
CTHR110
DTHR110
ETHR110
FTHR110

site_idSWS_FT_FI10
Number of Residues6
DetailsMOD_RES: N6-methyllysine; alternate => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
ALYS115
BLYS115
CLYS115
DLYS115
ELYS115
FLYS115

site_idSWS_FT_FI11
Number of Residues6
DetailsMOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
ATYR138
BTYR138
CTYR138
DTYR138
ETYR138
FTYR138

site_idSWS_FT_FI12
Number of Residues12
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P62157
ChainResidueDetails
ALYS21
FLYS21
BLYS21
CLYS21
DLYS21
ELYS21

227344

PDB entries from 2024-11-13

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