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8ODZ

Cryo-EM structure of a pre-dimerized murine IL-12 complete extracellular signaling complex (Class 1).

Functional Information from GO Data
ChainGOidnamespacecontents
A0001916biological_processpositive regulation of T cell mediated cytotoxicity
A0002860biological_processpositive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
A0005125molecular_functioncytokine activity
A0005143molecular_functioninterleukin-12 receptor binding
A0005515molecular_functionprotein binding
A0005576cellular_componentextracellular region
A0005615cellular_componentextracellular space
A0005737cellular_componentcytoplasm
A0005788cellular_componentendoplasmic reticulum lumen
A0005796cellular_componentGolgi lumen
A0006955biological_processimmune response
A0008083molecular_functiongrowth factor activity
A0008283biological_processcell population proliferation
A0009986cellular_componentcell surface
A0010224biological_processresponse to UV-B
A0016477biological_processcell migration
A0031904cellular_componentendosome lumen
A0032496biological_processresponse to lipopolysaccharide
A0032700biological_processnegative regulation of interleukin-17 production
A0032729biological_processpositive regulation of type II interferon production
A0032816biological_processpositive regulation of natural killer cell activation
A0032819biological_processpositive regulation of natural killer cell proliferation
A0032946biological_processpositive regulation of mononuclear cell proliferation
A0034393biological_processpositive regulation of smooth muscle cell apoptotic process
A0035711biological_processT-helper 1 cell activation
A0035722biological_processinterleukin-12-mediated signaling pathway
A0042022cellular_componentinterleukin-12 receptor complex
A0042102biological_processpositive regulation of T cell proliferation
A0042163molecular_functioninterleukin-12 beta subunit binding
A0042531biological_processpositive regulation of tyrosine phosphorylation of STAT protein
A0042832biological_processdefense response to protozoan
A0043514cellular_componentinterleukin-12 complex
A0045063biological_processT-helper 1 cell differentiation
A0045513molecular_functioninterleukin-27 binding
A0045582biological_processpositive regulation of T cell differentiation
A0045785biological_processpositive regulation of cell adhesion
A0045954biological_processpositive regulation of natural killer cell mediated cytotoxicity
A0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
A0046982molecular_functionprotein heterodimerization activity
A0048662biological_processnegative regulation of smooth muscle cell proliferation
A0050671biological_processpositive regulation of lymphocyte proliferation
A0050709biological_processnegative regulation of protein secretion
A0051135biological_processpositive regulation of NK T cell activation
A0051142biological_processpositive regulation of NK T cell proliferation
A0070745cellular_componentinterleukin-35 complex
A0071222biological_processcellular response to lipopolysaccharide
A0097191biological_processextrinsic apoptotic signaling pathway
A0098586biological_processcellular response to virus
A1900747biological_processnegative regulation of vascular endothelial growth factor signaling pathway
A1903588biological_processnegative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis
A2000510biological_processpositive regulation of dendritic cell chemotaxis
B0001912biological_processpositive regulation of leukocyte mediated cytotoxicity
B0001916biological_processpositive regulation of T cell mediated cytotoxicity
B0002230biological_processpositive regulation of defense response to virus by host
B0002323biological_processnatural killer cell activation involved in immune response
B0002708biological_processpositive regulation of lymphocyte mediated immunity
B0002827biological_processpositive regulation of T-helper 1 type immune response
B0002860biological_processpositive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
B0002862biological_processnegative regulation of inflammatory response to antigenic stimulus
B0004896molecular_functioncytokine receptor activity
B0005125molecular_functioncytokine activity
B0005126molecular_functioncytokine receptor binding
B0005143molecular_functioninterleukin-12 receptor binding
B0005515molecular_functionprotein binding
B0005576cellular_componentextracellular region
B0005615cellular_componentextracellular space
B0005737cellular_componentcytoplasm
B0005788cellular_componentendoplasmic reticulum lumen
B0005796cellular_componentGolgi lumen
B0005886cellular_componentplasma membrane
B0007166biological_processcell surface receptor signaling pathway
B0007259biological_processcell surface receptor signaling pathway via JAK-STAT
B0008083molecular_functiongrowth factor activity
B0008283biological_processcell population proliferation
B0009986cellular_componentcell surface
B0010224biological_processresponse to UV-B
B0016020cellular_componentmembrane
B0016477biological_processcell migration
B0030101biological_processnatural killer cell activation
B0031904cellular_componentendosome lumen
B0032693biological_processnegative regulation of interleukin-10 production
B0032700biological_processnegative regulation of interleukin-17 production
B0032725biological_processpositive regulation of granulocyte macrophage colony-stimulating factor production
B0032729biological_processpositive regulation of type II interferon production
B0032733biological_processpositive regulation of interleukin-10 production
B0032735biological_processpositive regulation of interleukin-12 production
B0032740biological_processpositive regulation of interleukin-17 production
B0032760biological_processpositive regulation of tumor necrosis factor production
B0032816biological_processpositive regulation of natural killer cell activation
B0032819biological_processpositive regulation of natural killer cell proliferation
B0032946biological_processpositive regulation of mononuclear cell proliferation
B0034393biological_processpositive regulation of smooth muscle cell apoptotic process
B0035722biological_processinterleukin-12-mediated signaling pathway
B0038155biological_processinterleukin-23-mediated signaling pathway
B0042022cellular_componentinterleukin-12 receptor complex
B0042093biological_processT-helper cell differentiation
B0042102biological_processpositive regulation of T cell proliferation
B0042104biological_processpositive regulation of activated T cell proliferation
B0042110biological_processT cell activation
B0042164molecular_functioninterleukin-12 alpha subunit binding
B0042802molecular_functionidentical protein binding
B0042832biological_processdefense response to protozoan
B0043514cellular_componentinterleukin-12 complex
B0044877molecular_functionprotein-containing complex binding
B0045063biological_processT-helper 1 cell differentiation
B0045519molecular_functioninterleukin-23 receptor binding
B0045672biological_processpositive regulation of osteoclast differentiation
B0045785biological_processpositive regulation of cell adhesion
B0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
B0046982molecular_functionprotein heterodimerization activity
B0048662biological_processnegative regulation of smooth muscle cell proliferation
B0050671biological_processpositive regulation of lymphocyte proliferation
B0050709biological_processnegative regulation of protein secretion
B0050727biological_processregulation of inflammatory response
B0050829biological_processdefense response to Gram-negative bacterium
B0051135biological_processpositive regulation of NK T cell activation
B0051142biological_processpositive regulation of NK T cell proliferation
B0051607biological_processdefense response to virus
B0070743cellular_componentinterleukin-23 complex
B0071222biological_processcellular response to lipopolysaccharide
B0071346biological_processcellular response to type II interferon
B0072536cellular_componentinterleukin-23 receptor complex
B1900747biological_processnegative regulation of vascular endothelial growth factor signaling pathway
B1903588biological_processnegative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis
B2000318biological_processpositive regulation of T-helper 17 type immune response
D0000086biological_processG2/M transition of mitotic cell cycle
D0000785cellular_componentchromatin
D0000922cellular_componentspindle pole
D0001975biological_processresponse to amphetamine
D0002027biological_processregulation of heart rate
D0004896molecular_functioncytokine receptor activity
D0005246molecular_functioncalcium channel regulator activity
D0005509molecular_functioncalcium ion binding
D0005513biological_processdetection of calcium ion
D0005515molecular_functionprotein binding
D0005634cellular_componentnucleus
D0005654cellular_componentnucleoplasm
D0005737cellular_componentcytoplasm
D0005813cellular_componentcentrosome
D0005819cellular_componentspindle
D0005829cellular_componentcytosol
D0005876cellular_componentspindle microtubule
D0006897biological_processendocytosis
D0007259biological_processcell surface receptor signaling pathway via JAK-STAT
D0008076cellular_componentvoltage-gated potassium channel complex
D0008179molecular_functionadenylate cyclase binding
D0010856molecular_functionadenylate cyclase activator activity
D0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
D0010881biological_processregulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
D0016020cellular_componentmembrane
D0016240biological_processautophagosome membrane docking
D0019855molecular_functioncalcium channel inhibitor activity
D0019901molecular_functionprotein kinase binding
D0019904molecular_functionprotein domain specific binding
D0030017cellular_componentsarcomere
D0030235molecular_functionnitric-oxide synthase regulator activity
D0030426cellular_componentgrowth cone
D0030672cellular_componentsynaptic vesicle membrane
D0031432molecular_functiontitin binding
D0031514cellular_componentmotile cilium
D0031800molecular_functiontype 3 metabotropic glutamate receptor binding
D0031966cellular_componentmitochondrial membrane
D0032465biological_processregulation of cytokinesis
D0032991cellular_componentprotein-containing complex
D0034704cellular_componentcalcium channel complex
D0035458biological_processcellular response to interferon-beta
D0043209cellular_componentmyelin sheath
D0043539molecular_functionprotein serine/threonine kinase activator activity
D0043548molecular_functionphosphatidylinositol 3-kinase binding
D0044305cellular_componentcalyx of Held
D0044325molecular_functiontransmembrane transporter binding
D0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
D0046872molecular_functionmetal ion binding
D0048306molecular_functioncalcium-dependent protein binding
D0050998molecular_functionnitric-oxide synthase binding
D0051412biological_processresponse to corticosterone
D0051592biological_processresponse to calcium ion
D0051649biological_processestablishment of localization in cell
D0055117biological_processregulation of cardiac muscle contraction
D0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
D0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
D0071346biological_processcellular response to type II interferon
D0072542molecular_functionprotein phosphatase activator activity
D0090150biological_processestablishment of protein localization to membrane
D0090151biological_processestablishment of protein localization to mitochondrial membrane
D0097225cellular_componentsperm midpiece
D0097720biological_processcalcineurin-mediated signaling
D0098685cellular_componentSchaffer collateral - CA1 synapse
D0098901biological_processregulation of cardiac muscle cell action potential
D0099523cellular_componentpresynaptic cytosol
D0099524cellular_componentpostsynaptic cytosol
D0140056biological_processorganelle localization by membrane tethering
D0140238biological_processpresynaptic endocytosis
D0150034cellular_componentdistal axon
D1900242biological_processregulation of synaptic vesicle endocytosis
D1901842biological_processnegative regulation of high voltage-gated calcium channel activity
D1901844biological_processregulation of cell communication by electrical coupling involved in cardiac conduction
D1902494cellular_componentcatalytic complex
D1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
D2000300biological_processregulation of synaptic vesicle exocytosis
Functional Information from PROSITE/UniProt
site_idPS01354
Number of Residues42
DetailsHEMATOPO_REC_L_F3 Long hematopoietin receptor, soluble alpha chains family signature. IkPDPPknLqmkplknsq..VevsWeyPdSWstphsyFsLkFfV
ChainResidueDetails
BILE231-VAL272

site_idPS00018
Number of Residues13
DetailsEF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL
ChainResidueDetails
DASP664-LEU676
DASP700-PHE712
DASP737-LEU749
DASP773-PHE785

site_idPS01353
Number of Residues58
DetailsHEMATOPO_REC_L_F2 Long hematopoietin receptor, gp130 family signature. NskgaSapthinivdlcgtgllaphqvsaksenmdnil...VtWqp.PkkadsavreYi.VeW
ChainResidueDetails
DASN416-TRP473

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues30
DetailsGlycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]}
ChainResidueDetails

site_idSWS_FT_FI2
Number of Residues83
DetailsDomain: {"description":"Ig-like C2-type"}
ChainResidueDetails

site_idSWS_FT_FI3
Number of Residues194
DetailsDomain: {"description":"Fibronectin type-III 3","evidences":[{"source":"PROSITE-ProRule","id":"PRU00316","evidenceCode":"ECO:0000255"}]}
ChainResidueDetails

site_idSWS_FT_FI4
Number of Residues8
DetailsMotif: {"description":"WSXWS motif"}
ChainResidueDetails

site_idSWS_FT_FI5
Number of Residues98
DetailsDomain: {"description":"Fibronectin type-III 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00316","evidenceCode":"ECO:0000255"}]}
ChainResidueDetails

site_idSWS_FT_FI6
Number of Residues93
DetailsDomain: {"description":"Fibronectin type-III 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00316","evidenceCode":"ECO:0000255"}]}
ChainResidueDetails

site_idSWS_FT_FI7
Number of Residues92
DetailsDomain: {"description":"Fibronectin type-III 4","evidences":[{"source":"PROSITE-ProRule","id":"PRU00316","evidenceCode":"ECO:0000255"}]}
ChainResidueDetails

246905

PDB entries from 2025-12-31

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