8KHP

CULLIN3-KLHL22-RBX1 E3 ligase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000070	biological_process	mitotic sister chromatid segregation
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005764	cellular_component	lysosome
A	0005813	cellular_component	centrosome
A	0005819	cellular_component	spindle
A	0005827	cellular_component	polar microtubule
A	0005829	cellular_component	cytosol
A	0005856	cellular_component	cytoskeleton
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0006513	biological_process	protein monoubiquitination
A	0007094	biological_process	mitotic spindle assembly checkpoint signaling
A	0010507	biological_process	negative regulation of autophagy
A	0015630	cellular_component	microtubule cytoskeleton
A	0016567	biological_process	protein ubiquitination
A	0030307	biological_process	positive regulation of cell growth
A	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
A	0032480	biological_process	negative regulation of type I interferon production
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0043231	cellular_component	intracellular membrane-bounded organelle
A	0045171	cellular_component	intercellular bridge
A	0051301	biological_process	cell division
A	0071230	biological_process	cellular response to amino acid stimulus
A	0071233	biological_process	cellular response to L-leucine
A	0071889	molecular_function	14-3-3 protein binding
A	0072686	cellular_component	mitotic spindle
A	1904263	biological_process	positive regulation of TORC1 signaling
A	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
B	0000070	biological_process	mitotic sister chromatid segregation
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005764	cellular_component	lysosome
B	0005813	cellular_component	centrosome
B	0005819	cellular_component	spindle
B	0005827	cellular_component	polar microtubule
B	0005829	cellular_component	cytosol
B	0005856	cellular_component	cytoskeleton
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0006513	biological_process	protein monoubiquitination
B	0007094	biological_process	mitotic spindle assembly checkpoint signaling
B	0010507	biological_process	negative regulation of autophagy
B	0015630	cellular_component	microtubule cytoskeleton
B	0016567	biological_process	protein ubiquitination
B	0030307	biological_process	positive regulation of cell growth
B	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
B	0032480	biological_process	negative regulation of type I interferon production
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0043231	cellular_component	intracellular membrane-bounded organelle
B	0045171	cellular_component	intercellular bridge
B	0051301	biological_process	cell division
B	0071230	biological_process	cellular response to amino acid stimulus
B	0071233	biological_process	cellular response to L-leucine
B	0071889	molecular_function	14-3-3 protein binding
B	0072686	cellular_component	mitotic spindle
B	1904263	biological_process	positive regulation of TORC1 signaling
B	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
C	0000082	biological_process	G1/S transition of mitotic cell cycle
C	0000122	biological_process	negative regulation of transcription by RNA polymerase II
C	0000209	biological_process	protein polyubiquitination
C	0000278	biological_process	mitotic cell cycle
C	0000902	biological_process	cell morphogenesis
C	0000922	cellular_component	spindle pole
C	0001701	biological_process	in utero embryonic development
C	0001831	biological_process	trophectodermal cellular morphogenesis
C	0004842	molecular_function	ubiquitin-protein transferase activity
C	0005112	molecular_function	Notch binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005794	cellular_component	Golgi apparatus
C	0005813	cellular_component	centrosome
C	0005819	cellular_component	spindle
C	0005827	cellular_component	polar microtubule
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0005886	cellular_component	plasma membrane
C	0006357	biological_process	regulation of transcription by RNA polymerase II
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0006513	biological_process	protein monoubiquitination
C	0006888	biological_process	endoplasmic reticulum to Golgi vesicle-mediated transport
C	0006954	biological_process	inflammatory response
C	0007080	biological_process	mitotic metaphase chromosome alignment
C	0007229	biological_process	integrin-mediated signaling pathway
C	0007369	biological_process	gastrulation
C	0008284	biological_process	positive regulation of cell population proliferation
C	0010467	biological_process	gene expression
C	0016020	cellular_component	membrane
C	0016055	biological_process	Wnt signaling pathway
C	0016192	biological_process	vesicle-mediated transport
C	0016477	biological_process	cell migration
C	0016567	biological_process	protein ubiquitination
C	0017145	biological_process	stem cell division
C	0030030	biological_process	cell projection organization
C	0030163	biological_process	protein catabolic process
C	0030332	molecular_function	cyclin binding
C	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
C	0031208	molecular_function	POZ domain binding
C	0031398	biological_process	positive regulation of protein ubiquitination
C	0031461	cellular_component	cullin-RING ubiquitin ligase complex
C	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
C	0031514	cellular_component	motile cilium
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0031648	biological_process	protein destabilization
C	0032467	biological_process	positive regulation of cytokinesis
C	0032480	biological_process	negative regulation of type I interferon production
C	0035024	biological_process	negative regulation of Rho protein signal transduction
C	0036126	cellular_component	sperm flagellum
C	0040016	biological_process	embryonic cleavage
C	0042802	molecular_function	identical protein binding
C	0043149	biological_process	stress fiber assembly
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0044346	biological_process	fibroblast apoptotic process
C	0045842	biological_process	positive regulation of mitotic metaphase/anaphase transition
C	0048208	biological_process	COPII vesicle coating
C	0051301	biological_process	cell division
C	0051865	biological_process	protein autoubiquitination
C	0061630	molecular_function	ubiquitin protein ligase activity
C	0070062	cellular_component	extracellular exosome
C	0070936	biological_process	protein K48-linked ubiquitination
C	0071230	biological_process	cellular response to amino acid stimulus
C	0071630	biological_process	nuclear protein quality control by the ubiquitin-proteasome system
C	0072576	biological_process	liver morphogenesis
C	0072686	cellular_component	mitotic spindle
C	0097193	biological_process	intrinsic apoptotic signaling pathway
C	0098794	cellular_component	postsynapse
C	0098978	cellular_component	glutamatergic synapse
C	0140252	biological_process	regulation protein catabolic process at postsynapse
C	0160072	molecular_function	ubiquitin ligase complex scaffold activity
C	1900076	biological_process	regulation of cellular response to insulin stimulus
C	1901992	biological_process	positive regulation of mitotic cell cycle phase transition
C	1904263	biological_process	positive regulation of TORC1 signaling
D	0000082	biological_process	G1/S transition of mitotic cell cycle
D	0000122	biological_process	negative regulation of transcription by RNA polymerase II
D	0000209	biological_process	protein polyubiquitination
D	0000278	biological_process	mitotic cell cycle
D	0000902	biological_process	cell morphogenesis
D	0000922	cellular_component	spindle pole
D	0001701	biological_process	in utero embryonic development
D	0001831	biological_process	trophectodermal cellular morphogenesis
D	0004842	molecular_function	ubiquitin-protein transferase activity
D	0005112	molecular_function	Notch binding
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005794	cellular_component	Golgi apparatus
D	0005813	cellular_component	centrosome
D	0005819	cellular_component	spindle
D	0005827	cellular_component	polar microtubule
D	0005829	cellular_component	cytosol
D	0005856	cellular_component	cytoskeleton
D	0005886	cellular_component	plasma membrane
D	0006357	biological_process	regulation of transcription by RNA polymerase II
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0006513	biological_process	protein monoubiquitination
D	0006888	biological_process	endoplasmic reticulum to Golgi vesicle-mediated transport
D	0006954	biological_process	inflammatory response
D	0007080	biological_process	mitotic metaphase chromosome alignment
D	0007229	biological_process	integrin-mediated signaling pathway
D	0007369	biological_process	gastrulation
D	0008284	biological_process	positive regulation of cell population proliferation
D	0010467	biological_process	gene expression
D	0016020	cellular_component	membrane
D	0016055	biological_process	Wnt signaling pathway
D	0016192	biological_process	vesicle-mediated transport
D	0016477	biological_process	cell migration
D	0016567	biological_process	protein ubiquitination
D	0017145	biological_process	stem cell division
D	0030030	biological_process	cell projection organization
D	0030163	biological_process	protein catabolic process
D	0030332	molecular_function	cyclin binding
D	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
D	0031208	molecular_function	POZ domain binding
D	0031398	biological_process	positive regulation of protein ubiquitination
D	0031461	cellular_component	cullin-RING ubiquitin ligase complex
D	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
D	0031514	cellular_component	motile cilium
D	0031625	molecular_function	ubiquitin protein ligase binding
D	0031648	biological_process	protein destabilization
D	0032467	biological_process	positive regulation of cytokinesis
D	0032480	biological_process	negative regulation of type I interferon production
D	0035024	biological_process	negative regulation of Rho protein signal transduction
D	0036126	cellular_component	sperm flagellum
D	0040016	biological_process	embryonic cleavage
D	0042802	molecular_function	identical protein binding
D	0043149	biological_process	stress fiber assembly
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0044346	biological_process	fibroblast apoptotic process
D	0045842	biological_process	positive regulation of mitotic metaphase/anaphase transition
D	0048208	biological_process	COPII vesicle coating
D	0051301	biological_process	cell division
D	0051865	biological_process	protein autoubiquitination
D	0061630	molecular_function	ubiquitin protein ligase activity
D	0070062	cellular_component	extracellular exosome
D	0070936	biological_process	protein K48-linked ubiquitination
D	0071230	biological_process	cellular response to amino acid stimulus
D	0071630	biological_process	nuclear protein quality control by the ubiquitin-proteasome system
D	0072576	biological_process	liver morphogenesis
D	0072686	cellular_component	mitotic spindle
D	0097193	biological_process	intrinsic apoptotic signaling pathway
D	0098794	cellular_component	postsynapse
D	0098978	cellular_component	glutamatergic synapse
D	0140252	biological_process	regulation protein catabolic process at postsynapse
D	0160072	molecular_function	ubiquitin ligase complex scaffold activity
D	1900076	biological_process	regulation of cellular response to insulin stimulus
D	1901992	biological_process	positive regulation of mitotic cell cycle phase transition
D	1904263	biological_process	positive regulation of TORC1 signaling
E	0000165	biological_process	MAPK cascade
E	0000209	biological_process	protein polyubiquitination
E	0004842	molecular_function	ubiquitin-protein transferase activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006281	biological_process	DNA repair
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0006513	biological_process	protein monoubiquitination
E	0006974	biological_process	DNA damage response
E	0007283	biological_process	spermatogenesis
E	0008270	molecular_function	zinc ion binding
E	0016567	biological_process	protein ubiquitination
E	0016740	molecular_function	transferase activity
E	0019005	cellular_component	SCF ubiquitin ligase complex
E	0019788	molecular_function	NEDD8 transferase activity
E	0030163	biological_process	protein catabolic process
E	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
E	0031461	cellular_component	cullin-RING ubiquitin ligase complex
E	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
E	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
E	0031464	cellular_component	Cul4A-RING E3 ubiquitin ligase complex
E	0031465	cellular_component	Cul4B-RING E3 ubiquitin ligase complex
E	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
E	0031467	cellular_component	Cul7-RING ubiquitin ligase complex
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
E	0032480	biological_process	negative regulation of type I interferon production
E	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
E	0034644	biological_process	cellular response to UV
E	0042110	biological_process	T cell activation
E	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0043687	biological_process	post-translational protein modification
E	0045116	biological_process	protein neddylation
E	0045732	biological_process	positive regulation of protein catabolic process
E	0046872	molecular_function	metal ion binding
E	0060090	molecular_function	molecular adaptor activity
E	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
E	0061630	molecular_function	ubiquitin protein ligase activity
E	0061663	molecular_function	NEDD8 ligase activity
E	0062197	biological_process	cellular response to chemical stress
E	0070936	biological_process	protein K48-linked ubiquitination
E	0071230	biological_process	cellular response to amino acid stimulus
E	0090090	biological_process	negative regulation of canonical Wnt signaling pathway
E	0097602	molecular_function	cullin family protein binding
E	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
E	1900076	biological_process	regulation of cellular response to insulin stimulus
E	1902499	biological_process	positive regulation of protein autoubiquitination
E	1902883	biological_process	negative regulation of response to oxidative stress
E	1904263	biological_process	positive regulation of TORC1 signaling
F	0000165	biological_process	MAPK cascade
F	0000209	biological_process	protein polyubiquitination
F	0004842	molecular_function	ubiquitin-protein transferase activity
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006281	biological_process	DNA repair
F	0006511	biological_process	ubiquitin-dependent protein catabolic process
F	0006513	biological_process	protein monoubiquitination
F	0006974	biological_process	DNA damage response
F	0007283	biological_process	spermatogenesis
F	0008270	molecular_function	zinc ion binding
F	0016567	biological_process	protein ubiquitination
F	0016740	molecular_function	transferase activity
F	0019005	cellular_component	SCF ubiquitin ligase complex
F	0019788	molecular_function	NEDD8 transferase activity
F	0030163	biological_process	protein catabolic process
F	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
F	0031461	cellular_component	cullin-RING ubiquitin ligase complex
F	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
F	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
F	0031464	cellular_component	Cul4A-RING E3 ubiquitin ligase complex
F	0031465	cellular_component	Cul4B-RING E3 ubiquitin ligase complex
F	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
F	0031467	cellular_component	Cul7-RING ubiquitin ligase complex
F	0031625	molecular_function	ubiquitin protein ligase binding
F	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
F	0032480	biological_process	negative regulation of type I interferon production
F	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
F	0034644	biological_process	cellular response to UV
F	0042110	biological_process	T cell activation
F	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
F	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
F	0043687	biological_process	post-translational protein modification
F	0045116	biological_process	protein neddylation
F	0045732	biological_process	positive regulation of protein catabolic process
F	0046872	molecular_function	metal ion binding
F	0060090	molecular_function	molecular adaptor activity
F	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
F	0061630	molecular_function	ubiquitin protein ligase activity
F	0061663	molecular_function	NEDD8 ligase activity
F	0062197	biological_process	cellular response to chemical stress
F	0070936	biological_process	protein K48-linked ubiquitination
F	0071230	biological_process	cellular response to amino acid stimulus
F	0090090	biological_process	negative regulation of canonical Wnt signaling pathway
F	0097602	molecular_function	cullin family protein binding
F	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
F	1900076	biological_process	regulation of cellular response to insulin stimulus
F	1902499	biological_process	positive regulation of protein autoubiquitination
F	1902883	biological_process	negative regulation of response to oxidative stress
F	1904263	biological_process	positive regulation of TORC1 signaling

Functional Information from PROSITE/UniProt

site_id	PS01256
Number of Residues	28
Details	CULLIN_1 Cullin family signature. IKkrIegLIEReYLaRtpedrkvYtYvA

Chain	Residue	Details
C	ILE741-ALA768

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	90
Details	ZN_FING: RING-type => ECO:0000255|PROSITE-ProRule:PRU00175

Chain	Residue	Details
E	CYS53-ASN98
F	CYS53-ASN98

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site_id	SWS_FT_FI2
Number of Residues	22
Details	BINDING: BINDING => ECO:0000269|PubMed:11961546, ECO:0007744|PDB:1LDJ, ECO:0007744|PDB:1LDK, ECO:0007744|PDB:1U6G, ECO:0007744|PDB:2HYE, ECO:0007744|PDB:3DPL, ECO:0007744|PDB:3DQV, ECO:0007744|PDB:3RTR, ECO:0007744|PDB:4F52, ECO:0007744|PDB:4P5O

Chain	Residue	Details
E	CYS42
E	CYS94
E	ASP97
F	CYS42
F	CYS45
F	CYS53
F	CYS56
F	CYS68
F	CYS75
F	HIS77
F	HIS80
E	CYS45
F	HIS82
F	CYS94
F	ASP97
E	CYS53
E	CYS56
E	CYS68
E	CYS75
E	HIS77
E	HIS80
E	HIS82

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site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:11961546, ECO:0007744|PDB:1LDJ, ECO:0007744|PDB:1LDK, ECO:0007744|PDB:1U6G, ECO:0007744|PDB:2HYE, ECO:0007744|PDB:3DQV, ECO:0007744|PDB:3RTR, ECO:0007744|PDB:4F52, ECO:0007744|PDB:4P5O

Chain	Residue	Details
E	CYS83
F	CYS83
D	LYS712

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site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: N-acetylmethionine => ECO:0007744|PubMed:20068231

Chain	Residue	Details
E	MET1
F	MET1

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site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: N-acetylalanine; in E3 ubiquitin-protein ligase RBX1, N-terminally processed => ECO:0000269|Ref.8, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378

Chain	Residue	Details
E	ALA2
F	ALA2

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site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163

Chain	Residue	Details
E	THR9
F	THR9

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