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cryo-EM structure of NTSR1-GRK2-Galpha(q) complexes 1

Functional Information from GO Data
ChainGOidnamespacecontents
G0001664molecular_functionG protein-coupled receptor binding
G0002026biological_processregulation of the force of heart contraction
G0002029biological_processdesensitization of G protein-coupled receptor signaling pathway
G0002031biological_processG protein-coupled receptor internalization
G0003108biological_processnegative regulation of the force of heart contraction by chemical signal
G0004672molecular_functionprotein kinase activity
G0004674molecular_functionprotein serine/threonine kinase activity
G0004703molecular_functionG protein-coupled receptor kinase activity
G0005515molecular_functionprotein binding
G0005524molecular_functionATP binding
G0005737cellular_componentcytoplasm
G0005829cellular_componentcytosol
G0005886cellular_componentplasma membrane
G0006468biological_processprotein phosphorylation
G0006886biological_processintracellular protein transport
G0007165biological_processsignal transduction
G0007186biological_processG protein-coupled receptor signaling pathway
G0007213biological_processG protein-coupled acetylcholine receptor signaling pathway
G0009966biological_processregulation of signal transduction
G0016020cellular_componentmembrane
G0016310biological_processphosphorylation
G0018105biological_processpeptidyl-serine phosphorylation
G0031694molecular_functionalpha-2A adrenergic receptor binding
G0031755molecular_functionEdg-2 lysophosphatidic acid receptor binding
G0042995cellular_componentcell projection
G0045202cellular_componentsynapse
G0045880biological_processpositive regulation of smoothened signaling pathway
G0045988biological_processnegative regulation of striated muscle contraction
G0047696molecular_functionbeta-adrenergic receptor kinase activity
G0060048biological_processcardiac muscle contraction
G0098793cellular_componentpresynapse
G0098794cellular_componentpostsynapse
G1901081biological_processnegative regulation of relaxation of smooth muscle
G1903566biological_processpositive regulation of protein localization to cilium
Q0001508biological_processaction potential
Q0001664molecular_functionG protein-coupled receptor binding
Q0001750cellular_componentphotoreceptor outer segment
Q0003924molecular_functionGTPase activity
Q0003925molecular_functionG protein activity
Q0005096molecular_functionGTPase activator activity
Q0005515molecular_functionprotein binding
Q0005525molecular_functionGTP binding
Q0005634cellular_componentnucleus
Q0005737cellular_componentcytoplasm
Q0005765cellular_componentlysosomal membrane
Q0005794cellular_componentGolgi apparatus
Q0005834cellular_componentheterotrimeric G-protein complex
Q0005886cellular_componentplasma membrane
Q0006469biological_processnegative regulation of protein kinase activity
Q0007165biological_processsignal transduction
Q0007186biological_processG protein-coupled receptor signaling pathway
Q0007189biological_processadenylate cyclase-activating G protein-coupled receptor signaling pathway
Q0007200biological_processphospholipase C-activating G protein-coupled receptor signaling pathway
Q0007202biological_processobsolete activation of phospholipase C activity
Q0007206biological_processphospholipase C-activating G protein-coupled glutamate receptor signaling pathway
Q0007208biological_processphospholipase C-activating serotonin receptor signaling pathway
Q0007213biological_processG protein-coupled acetylcholine receptor signaling pathway
Q0007215biological_processglutamate receptor signaling pathway
Q0007218biological_processneuropeptide signaling pathway
Q0007596biological_processblood coagulation
Q0007603biological_processphototransduction, visible light
Q0009649biological_processentrainment of circadian clock
Q0010543biological_processregulation of platelet activation
Q0016787molecular_functionhydrolase activity
Q0019001molecular_functionguanyl nucleotide binding
Q0030234molecular_functionenzyme regulator activity
Q0031683molecular_functionG-protein beta/gamma-subunit complex binding
Q0031965cellular_componentnuclear membrane
Q0034695biological_processresponse to prostaglandin E
Q0045202cellular_componentsynapse
Q0046872molecular_functionmetal ion binding
Q0050821biological_processprotein stabilization
Q0060158biological_processphospholipase C-activating dopamine receptor signaling pathway
Q0060828biological_processregulation of canonical Wnt signaling pathway
Q0070062cellular_componentextracellular exosome
R0001659biological_processtemperature homeostasis
R0003085biological_processnegative regulation of systemic arterial blood pressure
R0003254biological_processregulation of membrane depolarization
R0004930molecular_functionG protein-coupled receptor activity
R0005515molecular_functionprotein binding
R0005783cellular_componentendoplasmic reticulum
R0005794cellular_componentGolgi apparatus
R0005886cellular_componentplasma membrane
R0007186biological_processG protein-coupled receptor signaling pathway
R0007218biological_processneuropeptide signaling pathway
R0007268biological_processchemical synaptic transmission
R0007612biological_processlearning
R0008344biological_processadult locomotory behavior
R0009898cellular_componentcytoplasmic side of plasma membrane
R0009986cellular_componentcell surface
R0010628biological_processpositive regulation of gene expression
R0014049biological_processpositive regulation of glutamate secretion
R0014054biological_processpositive regulation of gamma-aminobutyric acid secretion
R0016020cellular_componentmembrane
R0016492molecular_functionG protein-coupled neurotensin receptor activity
R0030424cellular_componentaxon
R0030425cellular_componentdendrite
R0032280cellular_componentsymmetric synapse
R0033993biological_processresponse to lipid
R0042802molecular_functionidentical protein binding
R0043025cellular_componentneuronal cell body
R0043065biological_processpositive regulation of apoptotic process
R0043066biological_processnegative regulation of apoptotic process
R0043195cellular_componentterminal bouton
R0043197cellular_componentdendritic spine
R0043198cellular_componentdendritic shaft
R0043204cellular_componentperikaryon
R0043576biological_processregulation of respiratory gaseous exchange
R0043679cellular_componentaxon terminus
R0044309cellular_componentneuron spine
R0044877molecular_functionprotein-containing complex binding
R0045121cellular_componentmembrane raft
R0050965biological_processdetection of temperature stimulus involved in sensory perception of pain
R0051280biological_processnegative regulation of release of sequestered calcium ion into cytosol
R0051281biological_processpositive regulation of release of sequestered calcium ion into cytosol
R0060732biological_processpositive regulation of inositol phosphate biosynthetic process
R0070779biological_processD-aspartate import across plasma membrane
R0071545biological_processinositol phosphate catabolic process
R0090238biological_processpositive regulation of arachidonate secretion
R0097151biological_processpositive regulation of inhibitory postsynaptic potential
R0098712biological_processL-glutamate import across plasma membrane
Functional Information from PROSITE/UniProt
site_idPS00107
Number of Residues24
DetailsPROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. IGRGGFGEVYgCrkadtgkm..........YAMK
ChainResidueDetails
GILE197-LYS220

site_idPS00108
Number of Residues13
DetailsPROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VvYrDLKpaNILL
ChainResidueDetails
GVAL313-LEU325

site_idPS00237
Number of Residues17
DetailsG_PROTEIN_RECEP_F1_1 G-protein coupled receptors family 1 signature. ATAlNVASLSVERYLaI
ChainResidueDetails
RALA154-ILE170

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues130
DetailsTOPO_DOM: Extracellular => ECO:0000250|UniProtKB:P20789
ChainResidueDetails
RMET1-THR67
QTHR186
QASN274
QLYS275
QASP277
QALA331
RGLU123-ARG142
RTHR206-LYS234
RPHE326-HIS343
QTHR54
QSER156
QLEU180
QARG181
QARG183

site_idSWS_FT_FI2
Number of Residues20
DetailsTRANSMEM: Helical; Name=1 => ECO:0000250|UniProtKB:P20789
ChainResidueDetails
RALA68-LEU88
GLYS220

site_idSWS_FT_FI3
Number of Residues128
DetailsTOPO_DOM: Cytoplasmic => ECO:0000250|UniProtKB:P20789
ChainResidueDetails
RALA89-HIS102
RGLU165-ARG184
RALA260-ARG303
RASN365-TYR418

site_idSWS_FT_FI4
Number of Residues19
DetailsTRANSMEM: Helical; Name=2 => ECO:0000250|UniProtKB:P20789
ChainResidueDetails
RTYR103-VAL122

site_idSWS_FT_FI5
Number of Residues21
DetailsTRANSMEM: Helical; Name=3 => ECO:0000250|UniProtKB:P20789
ChainResidueDetails
RGLY143-VAL164

site_idSWS_FT_FI6
Number of Residues20
DetailsTRANSMEM: Helical; Name=4 => ECO:0000250|UniProtKB:P20789
ChainResidueDetails
RTHR185-PHE205

site_idSWS_FT_FI7
Number of Residues24
DetailsTRANSMEM: Helical; Name=5 => ECO:0000250|UniProtKB:P20789
ChainResidueDetails
RVAL235-ILE259

site_idSWS_FT_FI8
Number of Residues21
DetailsTRANSMEM: Helical; Name=6 => ECO:0000250|UniProtKB:P20789
ChainResidueDetails
RVAL304-MET325

site_idSWS_FT_FI9
Number of Residues20
DetailsTRANSMEM: Helical; Name=7 => ECO:0000250|UniProtKB:P20789
ChainResidueDetails
RTYR344-TYR364

site_idSWS_FT_FI10
Number of Residues2
DetailsLIPID: S-palmitoyl cysteine => ECO:0000305|PubMed:21725197
ChainResidueDetails
RCYS381
RCYS383

site_idSWS_FT_FI11
Number of Residues3
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255
ChainResidueDetails
RASN4
RASN37
RASN41

226707

PDB entries from 2024-10-30

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