8JO0

The Cryo-EM structure of a heptameric CED-4/CED-3 catalytic complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0006915	biological_process	apoptotic process
A	0006919	biological_process	activation of cysteine-type endopeptidase activity involved in apoptotic process
A	0008303	cellular_component	caspase complex
A	0008361	biological_process	regulation of cell size
A	0008656	molecular_function	cysteine-type endopeptidase activator activity involved in apoptotic process
A	0009792	biological_process	embryo development ending in birth or egg hatching
A	0010954	biological_process	positive regulation of protein processing
A	0016020	cellular_component	membrane
A	0016505	molecular_function	peptidase activator activity involved in apoptotic process
A	0030042	biological_process	actin filament depolymerization
A	0030155	biological_process	regulation of cell adhesion
A	0031647	biological_process	regulation of protein stability
A	0032991	cellular_component	protein-containing complex
A	0040034	biological_process	regulation of development, heterochronic
A	0042802	molecular_function	identical protein binding
A	0042981	biological_process	regulation of apoptotic process
A	0043065	biological_process	positive regulation of apoptotic process
A	0043531	molecular_function	ADP binding
A	0046716	biological_process	muscle cell cellular homeostasis
A	0046872	molecular_function	metal ion binding
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0048598	biological_process	embryonic morphogenesis
A	0050829	biological_process	defense response to Gram-negative bacterium
A	0051432	molecular_function	BH1 domain binding
A	0051434	molecular_function	BH3 domain binding
A	0061133	molecular_function	endopeptidase activator activity
A	0089720	molecular_function	caspase binding
A	0097202	biological_process	activation of cysteine-type endopeptidase activity
A	1900118	biological_process	negative regulation of execution phase of apoptosis
A	1902742	biological_process	apoptotic process involved in development
A	1904747	biological_process	positive regulation of apoptotic process involved in development
A	1905808	biological_process	positive regulation of synapse pruning
B	0000287	molecular_function	magnesium ion binding
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005829	cellular_component	cytosol
B	0006915	biological_process	apoptotic process
B	0006919	biological_process	activation of cysteine-type endopeptidase activity involved in apoptotic process
B	0008303	cellular_component	caspase complex
B	0008361	biological_process	regulation of cell size
B	0008656	molecular_function	cysteine-type endopeptidase activator activity involved in apoptotic process
B	0009792	biological_process	embryo development ending in birth or egg hatching
B	0010954	biological_process	positive regulation of protein processing
B	0016020	cellular_component	membrane
B	0016505	molecular_function	peptidase activator activity involved in apoptotic process
B	0030042	biological_process	actin filament depolymerization
B	0030155	biological_process	regulation of cell adhesion
B	0031647	biological_process	regulation of protein stability
B	0032991	cellular_component	protein-containing complex
B	0040034	biological_process	regulation of development, heterochronic
B	0042802	molecular_function	identical protein binding
B	0042981	biological_process	regulation of apoptotic process
B	0043065	biological_process	positive regulation of apoptotic process
B	0043531	molecular_function	ADP binding
B	0046716	biological_process	muscle cell cellular homeostasis
B	0046872	molecular_function	metal ion binding
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0048598	biological_process	embryonic morphogenesis
B	0050829	biological_process	defense response to Gram-negative bacterium
B	0051432	molecular_function	BH1 domain binding
B	0051434	molecular_function	BH3 domain binding
B	0061133	molecular_function	endopeptidase activator activity
B	0089720	molecular_function	caspase binding
B	0097202	biological_process	activation of cysteine-type endopeptidase activity
B	1900118	biological_process	negative regulation of execution phase of apoptosis
B	1902742	biological_process	apoptotic process involved in development
B	1904747	biological_process	positive regulation of apoptotic process involved in development
B	1905808	biological_process	positive regulation of synapse pruning
C	0000287	molecular_function	magnesium ion binding
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005829	cellular_component	cytosol
C	0006915	biological_process	apoptotic process
C	0006919	biological_process	activation of cysteine-type endopeptidase activity involved in apoptotic process
C	0008303	cellular_component	caspase complex
C	0008361	biological_process	regulation of cell size
C	0008656	molecular_function	cysteine-type endopeptidase activator activity involved in apoptotic process
C	0009792	biological_process	embryo development ending in birth or egg hatching
C	0010954	biological_process	positive regulation of protein processing
C	0016020	cellular_component	membrane
C	0016505	molecular_function	peptidase activator activity involved in apoptotic process
C	0030042	biological_process	actin filament depolymerization
C	0030155	biological_process	regulation of cell adhesion
C	0031647	biological_process	regulation of protein stability
C	0032991	cellular_component	protein-containing complex
C	0040034	biological_process	regulation of development, heterochronic
C	0042802	molecular_function	identical protein binding
C	0042981	biological_process	regulation of apoptotic process
C	0043065	biological_process	positive regulation of apoptotic process
C	0043531	molecular_function	ADP binding
C	0046716	biological_process	muscle cell cellular homeostasis
C	0046872	molecular_function	metal ion binding
C	0048471	cellular_component	perinuclear region of cytoplasm
C	0048598	biological_process	embryonic morphogenesis
C	0050829	biological_process	defense response to Gram-negative bacterium
C	0051432	molecular_function	BH1 domain binding
C	0051434	molecular_function	BH3 domain binding
C	0061133	molecular_function	endopeptidase activator activity
C	0089720	molecular_function	caspase binding
C	0097202	biological_process	activation of cysteine-type endopeptidase activity
C	1900118	biological_process	negative regulation of execution phase of apoptosis
C	1902742	biological_process	apoptotic process involved in development
C	1904747	biological_process	positive regulation of apoptotic process involved in development
C	1905808	biological_process	positive regulation of synapse pruning
D	0000287	molecular_function	magnesium ion binding
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005829	cellular_component	cytosol
D	0006915	biological_process	apoptotic process
D	0006919	biological_process	activation of cysteine-type endopeptidase activity involved in apoptotic process
D	0008303	cellular_component	caspase complex
D	0008361	biological_process	regulation of cell size
D	0008656	molecular_function	cysteine-type endopeptidase activator activity involved in apoptotic process
D	0009792	biological_process	embryo development ending in birth or egg hatching
D	0010954	biological_process	positive regulation of protein processing
D	0016020	cellular_component	membrane
D	0016505	molecular_function	peptidase activator activity involved in apoptotic process
D	0030042	biological_process	actin filament depolymerization
D	0030155	biological_process	regulation of cell adhesion
D	0031647	biological_process	regulation of protein stability
D	0032991	cellular_component	protein-containing complex
D	0040034	biological_process	regulation of development, heterochronic
D	0042802	molecular_function	identical protein binding
D	0042981	biological_process	regulation of apoptotic process
D	0043065	biological_process	positive regulation of apoptotic process
D	0043531	molecular_function	ADP binding
D	0046716	biological_process	muscle cell cellular homeostasis
D	0046872	molecular_function	metal ion binding
D	0048471	cellular_component	perinuclear region of cytoplasm
D	0048598	biological_process	embryonic morphogenesis
D	0050829	biological_process	defense response to Gram-negative bacterium
D	0051432	molecular_function	BH1 domain binding
D	0051434	molecular_function	BH3 domain binding
D	0061133	molecular_function	endopeptidase activator activity
D	0089720	molecular_function	caspase binding
D	0097202	biological_process	activation of cysteine-type endopeptidase activity
D	1900118	biological_process	negative regulation of execution phase of apoptosis
D	1902742	biological_process	apoptotic process involved in development
D	1904747	biological_process	positive regulation of apoptotic process involved in development
D	1905808	biological_process	positive regulation of synapse pruning
E	0000287	molecular_function	magnesium ion binding
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005739	cellular_component	mitochondrion
E	0005829	cellular_component	cytosol
E	0006915	biological_process	apoptotic process
E	0006919	biological_process	activation of cysteine-type endopeptidase activity involved in apoptotic process
E	0008303	cellular_component	caspase complex
E	0008361	biological_process	regulation of cell size
E	0008656	molecular_function	cysteine-type endopeptidase activator activity involved in apoptotic process
E	0009792	biological_process	embryo development ending in birth or egg hatching
E	0010954	biological_process	positive regulation of protein processing
E	0016020	cellular_component	membrane
E	0016505	molecular_function	peptidase activator activity involved in apoptotic process
E	0030042	biological_process	actin filament depolymerization
E	0030155	biological_process	regulation of cell adhesion
E	0031647	biological_process	regulation of protein stability
E	0032991	cellular_component	protein-containing complex
E	0040034	biological_process	regulation of development, heterochronic
E	0042802	molecular_function	identical protein binding
E	0042981	biological_process	regulation of apoptotic process
E	0043065	biological_process	positive regulation of apoptotic process
E	0043531	molecular_function	ADP binding
E	0046716	biological_process	muscle cell cellular homeostasis
E	0046872	molecular_function	metal ion binding
E	0048471	cellular_component	perinuclear region of cytoplasm
E	0048598	biological_process	embryonic morphogenesis
E	0050829	biological_process	defense response to Gram-negative bacterium
E	0051432	molecular_function	BH1 domain binding
E	0051434	molecular_function	BH3 domain binding
E	0061133	molecular_function	endopeptidase activator activity
E	0089720	molecular_function	caspase binding
E	0097202	biological_process	activation of cysteine-type endopeptidase activity
E	1900118	biological_process	negative regulation of execution phase of apoptosis
E	1902742	biological_process	apoptotic process involved in development
E	1904747	biological_process	positive regulation of apoptotic process involved in development
E	1905808	biological_process	positive regulation of synapse pruning
F	0000287	molecular_function	magnesium ion binding
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005739	cellular_component	mitochondrion
F	0005829	cellular_component	cytosol
F	0006915	biological_process	apoptotic process
F	0006919	biological_process	activation of cysteine-type endopeptidase activity involved in apoptotic process
F	0008303	cellular_component	caspase complex
F	0008361	biological_process	regulation of cell size
F	0008656	molecular_function	cysteine-type endopeptidase activator activity involved in apoptotic process
F	0009792	biological_process	embryo development ending in birth or egg hatching
F	0010954	biological_process	positive regulation of protein processing
F	0016020	cellular_component	membrane
F	0016505	molecular_function	peptidase activator activity involved in apoptotic process
F	0030042	biological_process	actin filament depolymerization
F	0030155	biological_process	regulation of cell adhesion
F	0031647	biological_process	regulation of protein stability
F	0032991	cellular_component	protein-containing complex
F	0040034	biological_process	regulation of development, heterochronic
F	0042802	molecular_function	identical protein binding
F	0042981	biological_process	regulation of apoptotic process
F	0043065	biological_process	positive regulation of apoptotic process
F	0043531	molecular_function	ADP binding
F	0046716	biological_process	muscle cell cellular homeostasis
F	0046872	molecular_function	metal ion binding
F	0048471	cellular_component	perinuclear region of cytoplasm
F	0048598	biological_process	embryonic morphogenesis
F	0050829	biological_process	defense response to Gram-negative bacterium
F	0051432	molecular_function	BH1 domain binding
F	0051434	molecular_function	BH3 domain binding
F	0061133	molecular_function	endopeptidase activator activity
F	0089720	molecular_function	caspase binding
F	0097202	biological_process	activation of cysteine-type endopeptidase activity
F	1900118	biological_process	negative regulation of execution phase of apoptosis
F	1902742	biological_process	apoptotic process involved in development
F	1904747	biological_process	positive regulation of apoptotic process involved in development
F	1905808	biological_process	positive regulation of synapse pruning
G	0000287	molecular_function	magnesium ion binding
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005739	cellular_component	mitochondrion
G	0005829	cellular_component	cytosol
G	0006915	biological_process	apoptotic process
G	0006919	biological_process	activation of cysteine-type endopeptidase activity involved in apoptotic process
G	0008303	cellular_component	caspase complex
G	0008361	biological_process	regulation of cell size
G	0008656	molecular_function	cysteine-type endopeptidase activator activity involved in apoptotic process
G	0009792	biological_process	embryo development ending in birth or egg hatching
G	0010954	biological_process	positive regulation of protein processing
G	0016020	cellular_component	membrane
G	0016505	molecular_function	peptidase activator activity involved in apoptotic process
G	0030042	biological_process	actin filament depolymerization
G	0030155	biological_process	regulation of cell adhesion
G	0031647	biological_process	regulation of protein stability
G	0032991	cellular_component	protein-containing complex
G	0040034	biological_process	regulation of development, heterochronic
G	0042802	molecular_function	identical protein binding
G	0042981	biological_process	regulation of apoptotic process
G	0043065	biological_process	positive regulation of apoptotic process
G	0043531	molecular_function	ADP binding
G	0046716	biological_process	muscle cell cellular homeostasis
G	0046872	molecular_function	metal ion binding
G	0048471	cellular_component	perinuclear region of cytoplasm
G	0048598	biological_process	embryonic morphogenesis
G	0050829	biological_process	defense response to Gram-negative bacterium
G	0051432	molecular_function	BH1 domain binding
G	0051434	molecular_function	BH3 domain binding
G	0061133	molecular_function	endopeptidase activator activity
G	0089720	molecular_function	caspase binding
G	0097202	biological_process	activation of cysteine-type endopeptidase activity
G	1900118	biological_process	negative regulation of execution phase of apoptosis
G	1902742	biological_process	apoptotic process involved in development
G	1904747	biological_process	positive regulation of apoptotic process involved in development
G	1905808	biological_process	positive regulation of synapse pruning
H	0042981	biological_process	regulation of apoptotic process
I	0042981	biological_process	regulation of apoptotic process
J	0042981	biological_process	regulation of apoptotic process
K	0042981	biological_process	regulation of apoptotic process
L	0042981	biological_process	regulation of apoptotic process
M	0042981	biological_process	regulation of apoptotic process

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	14
Details	BINDING: BINDING => ECO:0000269|PubMed:16208361, ECO:0000269|PubMed:20434985, ECO:0000269|PubMed:24065769, ECO:0007744|PDB:2A5Y, ECO:0007744|PDB:3LQQ, ECO:0007744|PDB:3LQR, ECO:0007744|PDB:4M9S, ECO:0007744|PDB:4M9X, ECO:0007744|PDB:4M9Y, ECO:0007744|PDB:4M9Z

Chain	Residue	Details
A	TYR131
E	GLY162
F	TYR131
F	GLY162
G	TYR131
G	GLY162
A	GLY162
B	TYR131
B	GLY162
C	TYR131
C	GLY162
D	TYR131
D	GLY162
E	TYR131

---

site_id	SWS_FT_FI2
Number of Residues	7
Details	BINDING: BINDING => ECO:0000269|PubMed:16208361, ECO:0000269|PubMed:20434985

Chain	Residue	Details
A	SER166
B	SER166
C	SER166
D	SER166
E	SER166
F	SER166
G	SER166

---

site_id	SWS_FT_FI3
Number of Residues	7
Details	BINDING: BINDING => ECO:0000269|PubMed:24065769, ECO:0007744|PDB:4M9S

Chain	Residue	Details
A	GLN171
B	GLN171
C	GLN171
D	GLN171
E	GLN171
F	GLN171
G	GLN171

---