8JG9

Cryo-EM structure of the SaCas9-sgRNA-AcrIIA15-promoter DNA dimer

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0003677	molecular_function	DNA binding
A	0003723	molecular_function	RNA binding
A	0004519	molecular_function	endonuclease activity
A	0043571	biological_process	maintenance of CRISPR repeat elements
A	0046872	molecular_function	metal ion binding
A	0051607	biological_process	defense response to virus
D	0003676	molecular_function	nucleic acid binding
D	0003677	molecular_function	DNA binding
D	0003723	molecular_function	RNA binding
D	0004519	molecular_function	endonuclease activity
D	0043571	biological_process	maintenance of CRISPR repeat elements
D	0046872	molecular_function	metal ion binding
D	0051607	biological_process	defense response to virus

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: For RuvC-like nuclease domain => ECO:0000305|PubMed:26317473

Chain	Residue	Details
A	ASP10
D	ASP10

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site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: Proton acceptor for HNH nuclease domain => ECO:0000305|PubMed:26317473

Chain	Residue	Details
A	HIS557
D	HIS557

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site_id	SWS_FT_FI3
Number of Residues	8
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01480

Chain	Residue	Details
A	ASP10
A	GLU477
A	GLU481
A	HIS701
D	ASP10
D	GLU477
D	GLU481
D	HIS701

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site_id	SWS_FT_FI4
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:26317473

Chain	Residue	Details
A	TYR789
D	TYR789

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