8JG9
Cryo-EM structure of the SaCas9-sgRNA-AcrIIA15-promoter DNA dimer
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003676 | molecular_function | nucleic acid binding |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0004518 | molecular_function | nuclease activity |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0043571 | biological_process | maintenance of CRISPR repeat elements |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0051607 | biological_process | defense response to virus |
| D | 0003676 | molecular_function | nucleic acid binding |
| D | 0003677 | molecular_function | DNA binding |
| D | 0003723 | molecular_function | RNA binding |
| D | 0004518 | molecular_function | nuclease activity |
| D | 0004519 | molecular_function | endonuclease activity |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0043571 | biological_process | maintenance of CRISPR repeat elements |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0051607 | biological_process | defense response to virus |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 332 |
| Details | Domain: {"description":"HNH Cas9-type","evidences":[{"source":"HAMAP-Rule","id":"MF_01480","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 80 |
| Details | Region: {"description":"RuvC-I","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 770 |
| Details | Region: {"description":"Recognition lobe","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 92 |
| Details | Region: {"description":"RuvC-II","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 30 |
| Details | Region: {"description":"PAM substrate-binding","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 286 |
| Details | Region: {"description":"PAM-interacting domain (PI)","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | Active site: {"description":"For RuvC-like nuclease domain","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Proton acceptor for HNH nuclease domain","evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 8 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01480","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"26317473","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
