8J1R

cryo-EM structures of Ufd4 in complex with Ubc4-Ub

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000209	biological_process	protein polyubiquitination
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0010994	biological_process	free ubiquitin chain polymerization
A	0016607	cellular_component	nuclear speck
A	0016740	molecular_function	transferase activity
A	0035519	biological_process	protein K29-linked ubiquitination
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0061630	molecular_function	ubiquitin protein ligase activity
A	1904855	molecular_function	proteasome regulatory particle binding
C	0000151	cellular_component	ubiquitin ligase complex
C	0000209	biological_process	protein polyubiquitination
C	0004842	molecular_function	ubiquitin-protein transferase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0016567	biological_process	protein ubiquitination
C	0016740	molecular_function	transferase activity
C	0030674	molecular_function	protein-macromolecule adaptor activity
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0034605	biological_process	cellular response to heat
C	0043130	molecular_function	ubiquitin binding
C	0043162	biological_process	ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
C	0061631	molecular_function	ubiquitin conjugating enzyme activity
C	0070628	molecular_function	proteasome binding
C	0071629	biological_process	cytoplasm protein quality control by the ubiquitin-proteasome system
C	0072344	biological_process	rescue of stalled ribosome
C	0072671	biological_process	mitochondria-associated ubiquitin-dependent protein catabolic process
C	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process

Functional Information from PROSITE/UniProt

site_id	PS00183
Number of Residues	16
Details	UBC_1 Ubiquitin-conjugating (UBC) active site signature. YHPNInan.GnICLdiL

Chain	Residue	Details
C	TYR75-LEU90

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Glycyl thioester intermediate => ECO:0000255|PROSITE-ProRule:PRU00388

Chain	Residue	Details
C	CYS86

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18407956

Chain	Residue	Details
C	SER12

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047

Chain	Residue	Details
C	LYS91

---