8J0O

cryo-EM structure of human EMC and VDAC

Functional Information from GO Data

Chain	GOid	namespace	contents
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005783	cellular_component	endoplasmic reticulum
B	0005789	cellular_component	endoplasmic reticulum membrane
B	0016020	cellular_component	membrane
B	0032977	molecular_function	membrane insertase activity
B	0042406	cellular_component	extrinsic component of endoplasmic reticulum membrane
B	0045050	biological_process	protein insertion into ER membrane by stop-transfer membrane-anchor sequence
B	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
B	0072546	cellular_component	EMC complex
C	0005515	molecular_function	protein binding
C	0005783	cellular_component	endoplasmic reticulum
C	0005789	cellular_component	endoplasmic reticulum membrane
C	0016020	cellular_component	membrane
C	0032977	molecular_function	membrane insertase activity
C	0045050	biological_process	protein insertion into ER membrane by stop-transfer membrane-anchor sequence
C	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
C	0072546	cellular_component	EMC complex
D	0005515	molecular_function	protein binding
D	0005783	cellular_component	endoplasmic reticulum
D	0005789	cellular_component	endoplasmic reticulum membrane
D	0006915	biological_process	apoptotic process
D	0016020	cellular_component	membrane
D	0032977	molecular_function	membrane insertase activity
D	0045050	biological_process	protein insertion into ER membrane by stop-transfer membrane-anchor sequence
D	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
D	0072546	cellular_component	EMC complex
E	0000139	cellular_component	Golgi membrane
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005768	cellular_component	endosome
E	0005769	cellular_component	early endosome
E	0005783	cellular_component	endoplasmic reticulum
E	0005789	cellular_component	endoplasmic reticulum membrane
E	0005794	cellular_component	Golgi apparatus
E	0005886	cellular_component	plasma membrane
E	0006812	biological_process	monoatomic cation transport
E	0006824	biological_process	cobalt ion transport
E	0006825	biological_process	copper ion transport
E	0006826	biological_process	iron ion transport
E	0015087	molecular_function	cobalt ion transmembrane transporter activity
E	0015093	molecular_function	ferrous iron transmembrane transporter activity
E	0015095	molecular_function	magnesium ion transmembrane transporter activity
E	0015693	biological_process	magnesium ion transport
E	0016020	cellular_component	membrane
E	0022890	molecular_function	inorganic cation transmembrane transporter activity
E	0031901	cellular_component	early endosome membrane
E	0032977	molecular_function	membrane insertase activity
E	0034755	biological_process	iron ion transmembrane transport
E	0043231	cellular_component	intracellular membrane-bounded organelle
E	0045050	biological_process	protein insertion into ER membrane by stop-transfer membrane-anchor sequence
E	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
E	0072546	cellular_component	EMC complex
E	1903830	biological_process	magnesium ion transmembrane transport
F	0000045	biological_process	autophagosome assembly
F	0005515	molecular_function	protein binding
F	0005783	cellular_component	endoplasmic reticulum
F	0005789	cellular_component	endoplasmic reticulum membrane
F	0016020	cellular_component	membrane
F	0032977	molecular_function	membrane insertase activity
F	0045050	biological_process	protein insertion into ER membrane by stop-transfer membrane-anchor sequence
F	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
F	0072546	cellular_component	EMC complex
F	1903349	cellular_component	omegasome membrane
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005783	cellular_component	endoplasmic reticulum
H	0005789	cellular_component	endoplasmic reticulum membrane
H	0005794	cellular_component	Golgi apparatus
H	0016020	cellular_component	membrane
H	0032977	molecular_function	membrane insertase activity
H	0045050	biological_process	protein insertion into ER membrane by stop-transfer membrane-anchor sequence
H	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
H	0072546	cellular_component	EMC complex
K	0001662	biological_process	behavioral fear response
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005739	cellular_component	mitochondrion
K	0005741	cellular_component	mitochondrial outer membrane
K	0005757	cellular_component	mitochondrial permeability transition pore complex
K	0005886	cellular_component	plasma membrane
K	0006090	biological_process	pyruvate metabolic process
K	0006811	biological_process	monoatomic ion transport
K	0006820	biological_process	monoatomic anion transport
K	0006915	biological_process	apoptotic process
K	0007268	biological_process	chemical synaptic transmission
K	0007270	biological_process	neuron-neuron synaptic transmission
K	0007612	biological_process	learning
K	0008142	molecular_function	oxysterol binding
K	0008289	molecular_function	lipid binding
K	0008308	molecular_function	voltage-gated monoatomic anion channel activity
K	0015288	molecular_function	porin activity
K	0015485	molecular_function	cholesterol binding
K	0016020	cellular_component	membrane
K	0019901	molecular_function	protein kinase binding
K	0030855	biological_process	epithelial cell differentiation
K	0031210	molecular_function	phosphatidylcholine binding
K	0031966	cellular_component	mitochondrial membrane
K	0036444	biological_process	calcium import into the mitochondrion
K	0042645	cellular_component	mitochondrial nucleoid
K	0042802	molecular_function	identical protein binding
K	0043065	biological_process	positive regulation of apoptotic process
K	0043066	biological_process	negative regulation of apoptotic process
K	0043227	cellular_component	membrane-bounded organelle
K	0044325	molecular_function	transmembrane transporter binding
K	0045121	cellular_component	membrane raft
K	0045202	cellular_component	synapse
K	0046930	cellular_component	pore complex
K	0070062	cellular_component	extracellular exosome
K	0097001	molecular_function	ceramide binding
K	0110099	biological_process	negative regulation of calcium import into the mitochondrion
K	1901524	biological_process	regulation of mitophagy
K	1903146	biological_process	regulation of autophagy of mitochondrion
K	1905091	biological_process	positive regulation of parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization
K	2000378	biological_process	negative regulation of reactive oxygen species metabolic process

Functional Information from PROSITE/UniProt

site_id	PS00558
Number of Residues	23
Details	EUKARYOTIC_PORIN Eukaryotic mitochondrial porin signature. YqiDPdAcfsAKVNNssliGLgY

Chain	Residue	Details
K	TYR225-TYR247

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	164
Details	TRANSMEM: Beta stranded => ECO:0000269|PubMed:18755977, ECO:0000269|PubMed:18832158

Chain	Residue	Details
K	VAL54-TRP64
K	LEU69-ASN76
K	THR80-ASP89
K	LEU95-SER104
K	ASN111-ARG120
K	ILE123-ASP130
K	SER137-GLY145
K	LEU150-GLU158
K	ARG163-THR175
K	PHE178-ASN185
K	GLU189-VAL198
K	LEU202-THR211
K	ARG218-ILE227
K	ALA231-ASN238
K	LEU242-LEU251
K	GLY254-LEU263
K	HIS273-GLN282
K	LEU26-SER35
K	LEU39-ALA47

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:18755977

Chain	Residue	Details
F	ILE90-MET106
K	LEU242
K	SER260

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	SITE: Involved in ceramide and phosphatidylcholine binding. Critical for channel structural stability and gating => ECO:0000269|PubMed:31015432, ECO:0000305|PubMed:18832158

Chain	Residue	Details
F	VAL107-TYR110
K	GLU73

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N-acetylalanine => ECO:0000269|PubMed:2559745, ECO:0000269|Ref.12, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378, ECO:0007744|PubMed:25944712

Chain	Residue	Details
A	ASN818
K	ALA2

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q9Z2L0

Chain	Residue	Details
K	SER13

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:Q60932

Chain	Residue	Details
K	THR19

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:Q60932

Chain	Residue	Details
K	LYS20

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:Q60932

Chain	Residue	Details
K	TYR67

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
K	THR107

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0000250|UniProtKB:Q60932

Chain	Residue	Details
K	LYS109

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	MOD_RES: Phosphoserine; by NEK1 => ECO:0000269|PubMed:20230784

Chain	Residue	Details
K	SER193

---

site_id	SWS_FT_FI12
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
K	SER240

---

site_id	SWS_FT_FI13
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q60932

Chain	Residue	Details
K	LYS252

---

site_id	SWS_FT_FI14
Number of Residues	1
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:19608861

Chain	Residue	Details
K	LYS266

---

site_id	SWS_FT_FI15
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:32047033

Chain	Residue	Details
K	LYS12

---

site_id	SWS_FT_FI16
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25621951, ECO:0000269|PubMed:32047033

Chain	Residue	Details
K	LYS53
K	LYS110
K	LYS274

---

site_id	SWS_FT_FI17
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000269|PubMed:32047033

Chain	Residue	Details
K	LYS20

---

site_id	SWS_FT_FI18
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25621951

Chain	Residue	Details
K	LYS61
K	LYS161

---

site_id	SWS_FT_FI19
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000269|PubMed:25621951, ECO:0000269|PubMed:32047033

Chain	Residue	Details
K	LYS109

---

site_id	SWS_FT_FI20
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000269|PubMed:25621951

Chain	Residue	Details
K	LYS266

---