8H3Q

Cryo-EM Structure of the CAND1-Cul3-Rbx1 complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000151	cellular_component	ubiquitin ligase complex
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005794	cellular_component	Golgi apparatus
A	0005829	cellular_component	cytosol
A	0010265	biological_process	SCF complex assembly
A	0016020	cellular_component	membrane
A	0016567	biological_process	protein ubiquitination
A	0017025	molecular_function	TBP-class protein binding
A	0030154	biological_process	cell differentiation
A	0031461	cellular_component	cullin-RING ubiquitin ligase complex
A	0034774	cellular_component	secretory granule lumen
A	0043086	biological_process	negative regulation of catalytic activity
A	0045893	biological_process	positive regulation of DNA-templated transcription
A	0045899	biological_process	positive regulation of RNA polymerase II transcription preinitiation complex assembly
A	0070062	cellular_component	extracellular exosome
A	1904813	cellular_component	ficolin-1-rich granule lumen
C	0000082	biological_process	G1/S transition of mitotic cell cycle
C	0000122	biological_process	negative regulation of transcription by RNA polymerase II
C	0000209	biological_process	protein polyubiquitination
C	0000278	biological_process	mitotic cell cycle
C	0000902	biological_process	cell morphogenesis
C	0000922	cellular_component	spindle pole
C	0001701	biological_process	in utero embryonic development
C	0001822	biological_process	kidney development
C	0001831	biological_process	trophectodermal cellular morphogenesis
C	0004842	molecular_function	ubiquitin-protein transferase activity
C	0005112	molecular_function	Notch binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005794	cellular_component	Golgi apparatus
C	0005813	cellular_component	centrosome
C	0005819	cellular_component	spindle
C	0005827	cellular_component	polar microtubule
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0005886	cellular_component	plasma membrane
C	0005929	cellular_component	cilium
C	0006357	biological_process	regulation of transcription by RNA polymerase II
C	0006366	biological_process	transcription by RNA polymerase II
C	0006368	biological_process	transcription elongation by RNA polymerase II
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0006513	biological_process	protein monoubiquitination
C	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
C	0006888	biological_process	endoplasmic reticulum to Golgi vesicle-mediated transport
C	0006915	biological_process	apoptotic process
C	0006950	biological_process	response to stress
C	0006954	biological_process	inflammatory response
C	0006979	biological_process	response to oxidative stress
C	0007080	biological_process	mitotic metaphase chromosome alignment
C	0007165	biological_process	signal transduction
C	0007229	biological_process	integrin-mediated signaling pathway
C	0007369	biological_process	gastrulation
C	0008284	biological_process	positive regulation of cell population proliferation
C	0010467	biological_process	gene expression
C	0010468	biological_process	regulation of gene expression
C	0010558	biological_process	negative regulation of macromolecule biosynthetic process
C	0016020	cellular_component	membrane
C	0016055	biological_process	Wnt signaling pathway
C	0016192	biological_process	vesicle-mediated transport
C	0016477	biological_process	cell migration
C	0016567	biological_process	protein ubiquitination
C	0017145	biological_process	stem cell division
C	0030030	biological_process	cell projection organization
C	0030163	biological_process	protein catabolic process
C	0030332	molecular_function	cyclin binding
C	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
C	0031208	molecular_function	POZ domain binding
C	0031398	biological_process	positive regulation of protein ubiquitination
C	0031461	cellular_component	cullin-RING ubiquitin ligase complex
C	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
C	0031514	cellular_component	motile cilium
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0031648	biological_process	protein destabilization
C	0031669	biological_process	cellular response to nutrient levels
C	0032467	biological_process	positive regulation of cytokinesis
C	0032480	biological_process	negative regulation of type I interferon production
C	0032481	biological_process	positive regulation of type I interferon production
C	0032869	biological_process	cellular response to insulin stimulus
C	0034198	biological_process	cellular response to amino acid starvation
C	0034599	biological_process	cellular response to oxidative stress
C	0035024	biological_process	negative regulation of Rho protein signal transduction
C	0036126	cellular_component	sperm flagellum
C	0040016	biological_process	embryonic cleavage
C	0042802	molecular_function	identical protein binding
C	0042995	cellular_component	cell projection
C	0043149	biological_process	stress fiber assembly
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0044346	biological_process	fibroblast apoptotic process
C	0045842	biological_process	positive regulation of mitotic metaphase/anaphase transition
C	0048208	biological_process	COPII vesicle coating
C	0051301	biological_process	cell division
C	0051865	biological_process	protein autoubiquitination
C	0060337	biological_process	type I interferon-mediated signaling pathway
C	0061630	molecular_function	ubiquitin protein ligase activity
C	0070062	cellular_component	extracellular exosome
C	0070294	biological_process	renal sodium ion absorption
C	0070936	biological_process	protein K48-linked ubiquitination
C	0071230	biological_process	cellular response to amino acid stimulus
C	0071630	biological_process	nuclear protein quality control by the ubiquitin-proteasome system
C	0072576	biological_process	liver morphogenesis
C	0072686	cellular_component	mitotic spindle
C	0080090	biological_process	regulation of primary metabolic process
C	0097193	biological_process	intrinsic apoptotic signaling pathway
C	0098794	cellular_component	postsynapse
C	0098978	cellular_component	glutamatergic synapse
C	0140252	biological_process	regulation protein catabolic process at postsynapse
C	0160072	molecular_function	ubiquitin ligase complex scaffold activity
C	0160240	biological_process	RNA polymerase II transcription initiation surveillance
C	1900076	biological_process	regulation of cellular response to insulin stimulus
C	1901992	biological_process	positive regulation of mitotic cell cycle phase transition
C	1904262	biological_process	negative regulation of TORC1 signaling
C	1904263	biological_process	positive regulation of TORC1 signaling
E	0000045	biological_process	autophagosome assembly
E	0000082	biological_process	G1/S transition of mitotic cell cycle
E	0000165	biological_process	MAPK cascade
E	0000209	biological_process	protein polyubiquitination
E	0000423	biological_process	mitophagy
E	0001837	biological_process	epithelial to mesenchymal transition
E	0004842	molecular_function	ubiquitin-protein transferase activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006281	biological_process	DNA repair
E	0006283	biological_process	transcription-coupled nucleotide-excision repair
E	0006366	biological_process	transcription by RNA polymerase II
E	0006368	biological_process	transcription elongation by RNA polymerase II
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0006513	biological_process	protein monoubiquitination
E	0006915	biological_process	apoptotic process
E	0006974	biological_process	DNA damage response
E	0006979	biological_process	response to oxidative stress
E	0007040	biological_process	lysosome organization
E	0007283	biological_process	spermatogenesis
E	0007346	biological_process	regulation of mitotic cell cycle
E	0008270	molecular_function	zinc ion binding
E	0008286	biological_process	insulin receptor signaling pathway
E	0010507	biological_process	negative regulation of autophagy
E	0010508	biological_process	positive regulation of autophagy
E	0016567	biological_process	protein ubiquitination
E	0016740	molecular_function	transferase activity
E	0019005	cellular_component	SCF ubiquitin ligase complex
E	0019221	biological_process	cytokine-mediated signaling pathway
E	0019788	molecular_function	NEDD8 transferase activity
E	0030163	biological_process	protein catabolic process
E	0030330	biological_process	DNA damage response, signal transduction by p53 class mediator
E	0030891	cellular_component	VCB complex
E	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
E	0031461	cellular_component	cullin-RING ubiquitin ligase complex
E	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
E	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
E	0031464	cellular_component	Cul4A-RING E3 ubiquitin ligase complex
E	0031465	cellular_component	Cul4B-RING E3 ubiquitin ligase complex
E	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
E	0031467	cellular_component	Cul7-RING ubiquitin ligase complex
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0031669	biological_process	cellular response to nutrient levels
E	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
E	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
E	0032480	biological_process	negative regulation of type I interferon production
E	0032481	biological_process	positive regulation of type I interferon production
E	0032869	biological_process	cellular response to insulin stimulus
E	0034198	biological_process	cellular response to amino acid starvation
E	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
E	0034599	biological_process	cellular response to oxidative stress
E	0034644	biological_process	cellular response to UV
E	0035279	biological_process	miRNA-mediated gene silencing by mRNA destabilization
E	0038066	biological_process	p38MAPK cascade
E	0038202	biological_process	TORC1 signaling
E	0042110	biological_process	T cell activation
E	0042770	biological_process	signal transduction in response to DNA damage
E	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
E	0043124	biological_process	negative regulation of canonical NF-kappaB signal transduction
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0043687	biological_process	post-translational protein modification
E	0044877	molecular_function	protein-containing complex binding
E	0045116	biological_process	protein neddylation
E	0045727	biological_process	positive regulation of translation
E	0045732	biological_process	positive regulation of protein catabolic process
E	0045944	biological_process	positive regulation of transcription by RNA polymerase II
E	0046627	biological_process	negative regulation of insulin receptor signaling pathway
E	0046872	molecular_function	metal ion binding
E	0060090	molecular_function	molecular adaptor activity
E	0060337	biological_process	type I interferon-mediated signaling pathway
E	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
E	0061630	molecular_function	ubiquitin protein ligase activity
E	0061663	molecular_function	NEDD8 ligase activity
E	0062197	biological_process	cellular response to chemical stress
E	0070294	biological_process	renal sodium ion absorption
E	0070936	biological_process	protein K48-linked ubiquitination
E	0071230	biological_process	cellular response to amino acid stimulus
E	0080008	cellular_component	Cul4-RING E3 ubiquitin ligase complex
E	0090090	biological_process	negative regulation of canonical Wnt signaling pathway
E	0090734	cellular_component	site of DNA damage
E	0097510	biological_process	base-excision repair, AP site formation via deaminated base removal
E	0097602	molecular_function	cullin family protein binding
E	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
E	0160240	biological_process	RNA polymerase II transcription initiation surveillance
E	1900076	biological_process	regulation of cellular response to insulin stimulus
E	1901525	biological_process	negative regulation of mitophagy
E	1901797	biological_process	negative regulation of signal transduction by p53 class mediator
E	1902499	biological_process	positive regulation of protein autoubiquitination
E	1902883	biological_process	negative regulation of response to oxidative stress
E	1904037	biological_process	positive regulation of epithelial cell apoptotic process
E	1904262	biological_process	negative regulation of TORC1 signaling
E	1904263	biological_process	positive regulation of TORC1 signaling
E	2000059	biological_process	negative regulation of ubiquitin-dependent protein catabolic process

Functional Information from PROSITE/UniProt

site_id	PS01256
Number of Residues	28
Details	CULLIN_1 Cullin family signature. IKkrIegLIEReYLaRtpedrkvYtYvA

Chain	Residue	Details
C	ILE741-ALA768

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	45
Details	ZN_FING: RING-type => ECO:0000255|PROSITE-ProRule:PRU00175

Chain	Residue	Details
E	CYS53-ASN98

---

site_id	SWS_FT_FI2
Number of Residues	7
Details	BINDING: BINDING => ECO:0000269|PubMed:11961546, ECO:0000269|PubMed:38605244, ECO:0007744|PDB:1LDJ, ECO:0007744|PDB:1LDK, ECO:0007744|PDB:1U6G, ECO:0007744|PDB:2HYE, ECO:0007744|PDB:3DPL, ECO:0007744|PDB:3DQV, ECO:0007744|PDB:3RTR, ECO:0007744|PDB:4F52, ECO:0007744|PDB:4P5O, ECO:0007744|PDB:8Q7H, ECO:0007744|PDB:8RHZ

Chain	Residue	Details
E	CYS42
E	CYS45
E	CYS75
E	HIS77
E	HIS80
E	CYS94
E	ASP97

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:11961546, ECO:0000269|PubMed:38605244, ECO:0007744|PDB:1LDJ, ECO:0007744|PDB:1LDK, ECO:0007744|PDB:1U6G, ECO:0007744|PDB:2HYE, ECO:0007744|PDB:3DPL, ECO:0007744|PDB:3DQV, ECO:0007744|PDB:3RTR, ECO:0007744|PDB:4F52, ECO:0007744|PDB:4P5O, ECO:0007744|PDB:7Z8B, ECO:0007744|PDB:8Q7H, ECO:0007744|PDB:8RHZ

Chain	Residue	Details
E	CYS53
E	CYS56
E	CYS68
E	HIS82

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:11961546, ECO:0000269|PubMed:38605244, ECO:0007744|PDB:1LDJ, ECO:0007744|PDB:1LDK, ECO:0007744|PDB:1U6G, ECO:0007744|PDB:2HYE, ECO:0007744|PDB:3DQV, ECO:0007744|PDB:3RTR, ECO:0007744|PDB:4F52, ECO:0007744|PDB:4P5O, ECO:0007744|PDB:8Q7H, ECO:0007744|PDB:8RHZ

Chain	Residue	Details
E	CYS83

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: N-acetylmethionine => ECO:0007744|PubMed:20068231

Chain	Residue	Details
E	MET1

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: N-acetylalanine; in E3 ubiquitin-protein ligase RBX1, N-terminally processed => ECO:0000269|Ref.8, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378

Chain	Residue	Details
E	ALA2

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163

Chain	Residue	Details
E	THR9

---