8GS5

Crystal structure of a constitutively active mutant of human IDH3 holoenzyme in apo form

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005739	cellular_component	mitochondrion
A	0005759	cellular_component	mitochondrial matrix
A	0005975	biological_process	carbohydrate metabolic process
A	0006099	biological_process	tricarboxylic acid cycle
A	0006102	biological_process	isocitrate metabolic process
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
A	0046872	molecular_function	metal ion binding
A	0051287	molecular_function	NAD binding
B	0000287	molecular_function	magnesium ion binding
B	0006099	biological_process	tricarboxylic acid cycle
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0051287	molecular_function	NAD binding
C	0000287	molecular_function	magnesium ion binding
C	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005739	cellular_component	mitochondrion
C	0005759	cellular_component	mitochondrial matrix
C	0005975	biological_process	carbohydrate metabolic process
C	0006099	biological_process	tricarboxylic acid cycle
C	0006102	biological_process	isocitrate metabolic process
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
C	0046872	molecular_function	metal ion binding
C	0051287	molecular_function	NAD binding
D	0000166	molecular_function	nucleotide binding
D	0000287	molecular_function	magnesium ion binding
D	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005730	cellular_component	nucleolus
D	0005739	cellular_component	mitochondrion
D	0005759	cellular_component	mitochondrial matrix
D	0005975	biological_process	carbohydrate metabolic process
D	0006099	biological_process	tricarboxylic acid cycle
D	0006102	biological_process	isocitrate metabolic process
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
D	0046872	molecular_function	metal ion binding
D	0051287	molecular_function	NAD binding
E	0000287	molecular_function	magnesium ion binding
E	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005739	cellular_component	mitochondrion
E	0005759	cellular_component	mitochondrial matrix
E	0005975	biological_process	carbohydrate metabolic process
E	0006099	biological_process	tricarboxylic acid cycle
E	0006102	biological_process	isocitrate metabolic process
E	0016491	molecular_function	oxidoreductase activity
E	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
E	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
E	0046872	molecular_function	metal ion binding
E	0051287	molecular_function	NAD binding
F	0000287	molecular_function	magnesium ion binding
F	0006099	biological_process	tricarboxylic acid cycle
F	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
F	0051287	molecular_function	NAD binding
G	0000287	molecular_function	magnesium ion binding
G	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005739	cellular_component	mitochondrion
G	0005759	cellular_component	mitochondrial matrix
G	0005975	biological_process	carbohydrate metabolic process
G	0006099	biological_process	tricarboxylic acid cycle
G	0006102	biological_process	isocitrate metabolic process
G	0016491	molecular_function	oxidoreductase activity
G	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
G	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
G	0046872	molecular_function	metal ion binding
G	0051287	molecular_function	NAD binding
H	0000166	molecular_function	nucleotide binding
H	0000287	molecular_function	magnesium ion binding
H	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005730	cellular_component	nucleolus
H	0005739	cellular_component	mitochondrion
H	0005759	cellular_component	mitochondrial matrix
H	0005975	biological_process	carbohydrate metabolic process
H	0006099	biological_process	tricarboxylic acid cycle
H	0006102	biological_process	isocitrate metabolic process
H	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
H	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
H	0046872	molecular_function	metal ion binding
H	0051287	molecular_function	NAD binding
I	0000287	molecular_function	magnesium ion binding
I	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005739	cellular_component	mitochondrion
I	0005759	cellular_component	mitochondrial matrix
I	0005975	biological_process	carbohydrate metabolic process
I	0006099	biological_process	tricarboxylic acid cycle
I	0006102	biological_process	isocitrate metabolic process
I	0016491	molecular_function	oxidoreductase activity
I	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
I	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
I	0046872	molecular_function	metal ion binding
I	0051287	molecular_function	NAD binding
J	0000287	molecular_function	magnesium ion binding
J	0006099	biological_process	tricarboxylic acid cycle
J	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
J	0051287	molecular_function	NAD binding
K	0000287	molecular_function	magnesium ion binding
K	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005739	cellular_component	mitochondrion
K	0005759	cellular_component	mitochondrial matrix
K	0005975	biological_process	carbohydrate metabolic process
K	0006099	biological_process	tricarboxylic acid cycle
K	0006102	biological_process	isocitrate metabolic process
K	0016491	molecular_function	oxidoreductase activity
K	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
K	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
K	0046872	molecular_function	metal ion binding
K	0051287	molecular_function	NAD binding
L	0000166	molecular_function	nucleotide binding
L	0000287	molecular_function	magnesium ion binding
L	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0005730	cellular_component	nucleolus
L	0005739	cellular_component	mitochondrion
L	0005759	cellular_component	mitochondrial matrix
L	0005975	biological_process	carbohydrate metabolic process
L	0006099	biological_process	tricarboxylic acid cycle
L	0006102	biological_process	isocitrate metabolic process
L	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
L	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
L	0046872	molecular_function	metal ion binding
L	0051287	molecular_function	NAD binding
M	0000287	molecular_function	magnesium ion binding
M	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
M	0005515	molecular_function	protein binding
M	0005634	cellular_component	nucleus
M	0005739	cellular_component	mitochondrion
M	0005759	cellular_component	mitochondrial matrix
M	0005975	biological_process	carbohydrate metabolic process
M	0006099	biological_process	tricarboxylic acid cycle
M	0006102	biological_process	isocitrate metabolic process
M	0016491	molecular_function	oxidoreductase activity
M	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
M	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
M	0046872	molecular_function	metal ion binding
M	0051287	molecular_function	NAD binding
N	0000287	molecular_function	magnesium ion binding
N	0006099	biological_process	tricarboxylic acid cycle
N	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
N	0051287	molecular_function	NAD binding
O	0000287	molecular_function	magnesium ion binding
O	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005739	cellular_component	mitochondrion
O	0005759	cellular_component	mitochondrial matrix
O	0005975	biological_process	carbohydrate metabolic process
O	0006099	biological_process	tricarboxylic acid cycle
O	0006102	biological_process	isocitrate metabolic process
O	0016491	molecular_function	oxidoreductase activity
O	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
O	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
O	0046872	molecular_function	metal ion binding
O	0051287	molecular_function	NAD binding
P	0000166	molecular_function	nucleotide binding
P	0000287	molecular_function	magnesium ion binding
P	0004449	molecular_function	isocitrate dehydrogenase (NAD+) activity
P	0005515	molecular_function	protein binding
P	0005524	molecular_function	ATP binding
P	0005730	cellular_component	nucleolus
P	0005739	cellular_component	mitochondrion
P	0005759	cellular_component	mitochondrial matrix
P	0005975	biological_process	carbohydrate metabolic process
P	0006099	biological_process	tricarboxylic acid cycle
P	0006102	biological_process	isocitrate metabolic process
P	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
P	0045242	cellular_component	isocitrate dehydrogenase complex (NAD+)
P	0046872	molecular_function	metal ion binding
P	0051287	molecular_function	NAD binding

Functional Information from PROSITE/UniProt

site_id	PS00470
Number of Residues	20
Details	IDH_IMDH Isocitrate and isopropylmalate dehydrogenases signature. NLYGNIiDNlaAglv.GGAGV

Chain	Residue	Details
B	ASN237-VAL256
C	ASN226-VAL245
D	ASN235-LEU254

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	79
Details	Binding site: {"evidences":[{"source":"PubMed","id":"28098230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Site: {"description":"Critical for catalysis","evidences":[{"source":"PubMed","id":"28098230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28139779","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Site: {"description":"Critical for catalysis","evidences":[{"source":"PubMed","id":"28098230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"Q9D6R2","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"Q9D6R2","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"Q9D6R2","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	12
Details	Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"Q9D6R2","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---