8G25

Crystal Structure of Cathepsin-G and Neutrophil Elastase Inhibited by S. aureus EapH2 at pH 7.5

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002003	biological_process	angiotensin maturation
A	0002548	biological_process	monocyte chemotaxis
A	0004252	molecular_function	serine-type endopeptidase activity
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005634	cellular_component	nucleus
A	0005764	cellular_component	lysosome
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006508	biological_process	proteolysis
A	0006955	biological_process	immune response
A	0008201	molecular_function	heparin binding
A	0008233	molecular_function	peptidase activity
A	0008236	molecular_function	serine-type peptidase activity
A	0010494	cellular_component	cytoplasmic stress granule
A	0016020	cellular_component	membrane
A	0016485	biological_process	protein processing
A	0019221	biological_process	cytokine-mediated signaling pathway
A	0019731	biological_process	antibacterial humoral response
A	0022617	biological_process	extracellular matrix disassembly
A	0030141	cellular_component	secretory granule
A	0030168	biological_process	platelet activation
A	0031012	cellular_component	extracellular matrix
A	0035578	cellular_component	azurophil granule lumen
A	0035590	biological_process	purinergic nucleotide receptor signaling pathway
A	0042119	biological_process	neutrophil activation
A	0048018	molecular_function	receptor ligand activity
A	0050829	biological_process	defense response to Gram-negative bacterium
A	0050830	biological_process	defense response to Gram-positive bacterium
A	0050868	biological_process	negative regulation of T cell activation
A	0051604	biological_process	protein maturation
A	0070062	cellular_component	extracellular exosome
A	0071222	biological_process	cellular response to lipopolysaccharide
A	0089720	molecular_function	caspase binding
A	0098786	biological_process	biofilm matrix disassembly
A	1901731	biological_process	positive regulation of platelet aggregation
B	0000122	biological_process	negative regulation of transcription by RNA polymerase II
B	0002020	molecular_function	protease binding
B	0002438	biological_process	acute inflammatory response to antigenic stimulus
B	0002523	biological_process	leukocyte migration involved in inflammatory response
B	0002812	biological_process	biosynthetic process of antibacterial peptides active against Gram-negative bacteria
B	0003714	molecular_function	transcription corepressor activity
B	0004175	molecular_function	endopeptidase activity
B	0004252	molecular_function	serine-type endopeptidase activity
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006508	biological_process	proteolysis
B	0006874	biological_process	intracellular calcium ion homeostasis
B	0006909	biological_process	phagocytosis
B	0008201	molecular_function	heparin binding
B	0008233	molecular_function	peptidase activity
B	0008236	molecular_function	serine-type peptidase activity
B	0009411	biological_process	response to UV
B	0009986	cellular_component	cell surface
B	0017053	cellular_component	transcription repressor complex
B	0019955	molecular_function	cytokine binding
B	0022617	biological_process	extracellular matrix disassembly
B	0030141	cellular_component	secretory granule
B	0030163	biological_process	protein catabolic process
B	0031012	cellular_component	extracellular matrix
B	0031452	biological_process	negative regulation of heterochromatin formation
B	0032682	biological_process	negative regulation of chemokine production
B	0032717	biological_process	negative regulation of interleukin-8 production
B	0032757	biological_process	positive regulation of interleukin-8 production
B	0035578	cellular_component	azurophil granule lumen
B	0035580	cellular_component	specific granule lumen
B	0042582	cellular_component	azurophil granule
B	0042742	biological_process	defense response to bacterium
B	0043406	biological_process	positive regulation of MAP kinase activity
B	0045335	cellular_component	phagocytic vesicle
B	0048661	biological_process	positive regulation of smooth muscle cell proliferation
B	0050728	biological_process	negative regulation of inflammatory response
B	0050922	biological_process	negative regulation of chemotaxis
B	0070062	cellular_component	extracellular exosome
B	0070269	biological_process	pyroptotic inflammatory response
B	0070943	biological_process	neutrophil-mediated killing of symbiont cell
B	0070944	biological_process	neutrophil-mediated killing of bacterium
B	0070945	biological_process	neutrophil-mediated killing of gram-negative bacterium
B	0070947	biological_process	neutrophil-mediated killing of fungus
B	0140644	cellular_component	neutrophil extracellular trap
B	0140645	biological_process	neutrophil extracellular trap formation
B	1903238	biological_process	positive regulation of leukocyte tethering or rolling
E	0000122	biological_process	negative regulation of transcription by RNA polymerase II
E	0002020	molecular_function	protease binding
E	0002438	biological_process	acute inflammatory response to antigenic stimulus
E	0002523	biological_process	leukocyte migration involved in inflammatory response
E	0002812	biological_process	biosynthetic process of antibacterial peptides active against Gram-negative bacteria
E	0003714	molecular_function	transcription corepressor activity
E	0004175	molecular_function	endopeptidase activity
E	0004252	molecular_function	serine-type endopeptidase activity
E	0005515	molecular_function	protein binding
E	0005576	cellular_component	extracellular region
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006508	biological_process	proteolysis
E	0006874	biological_process	intracellular calcium ion homeostasis
E	0006909	biological_process	phagocytosis
E	0008201	molecular_function	heparin binding
E	0008233	molecular_function	peptidase activity
E	0008236	molecular_function	serine-type peptidase activity
E	0009411	biological_process	response to UV
E	0009986	cellular_component	cell surface
E	0017053	cellular_component	transcription repressor complex
E	0019955	molecular_function	cytokine binding
E	0022617	biological_process	extracellular matrix disassembly
E	0030141	cellular_component	secretory granule
E	0030163	biological_process	protein catabolic process
E	0031012	cellular_component	extracellular matrix
E	0031452	biological_process	negative regulation of heterochromatin formation
E	0032682	biological_process	negative regulation of chemokine production
E	0032717	biological_process	negative regulation of interleukin-8 production
E	0032757	biological_process	positive regulation of interleukin-8 production
E	0035578	cellular_component	azurophil granule lumen
E	0035580	cellular_component	specific granule lumen
E	0042582	cellular_component	azurophil granule
E	0042742	biological_process	defense response to bacterium
E	0043406	biological_process	positive regulation of MAP kinase activity
E	0045335	cellular_component	phagocytic vesicle
E	0048661	biological_process	positive regulation of smooth muscle cell proliferation
E	0050728	biological_process	negative regulation of inflammatory response
E	0050922	biological_process	negative regulation of chemotaxis
E	0070062	cellular_component	extracellular exosome
E	0070269	biological_process	pyroptotic inflammatory response
E	0070943	biological_process	neutrophil-mediated killing of symbiont cell
E	0070944	biological_process	neutrophil-mediated killing of bacterium
E	0070945	biological_process	neutrophil-mediated killing of gram-negative bacterium
E	0070947	biological_process	neutrophil-mediated killing of fungus
E	0140644	cellular_component	neutrophil extracellular trap
E	0140645	biological_process	neutrophil extracellular trap formation
E	1903238	biological_process	positive regulation of leukocyte tethering or rolling
F	0002003	biological_process	angiotensin maturation
F	0002548	biological_process	monocyte chemotaxis
F	0004252	molecular_function	serine-type endopeptidase activity
F	0005515	molecular_function	protein binding
F	0005576	cellular_component	extracellular region
F	0005634	cellular_component	nucleus
F	0005764	cellular_component	lysosome
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0006508	biological_process	proteolysis
F	0006955	biological_process	immune response
F	0008201	molecular_function	heparin binding
F	0008233	molecular_function	peptidase activity
F	0008236	molecular_function	serine-type peptidase activity
F	0010494	cellular_component	cytoplasmic stress granule
F	0016020	cellular_component	membrane
F	0016485	biological_process	protein processing
F	0019221	biological_process	cytokine-mediated signaling pathway
F	0019731	biological_process	antibacterial humoral response
F	0022617	biological_process	extracellular matrix disassembly
F	0030141	cellular_component	secretory granule
F	0030168	biological_process	platelet activation
F	0031012	cellular_component	extracellular matrix
F	0035578	cellular_component	azurophil granule lumen
F	0035590	biological_process	purinergic nucleotide receptor signaling pathway
F	0042119	biological_process	neutrophil activation
F	0048018	molecular_function	receptor ligand activity
F	0050829	biological_process	defense response to Gram-negative bacterium
F	0050830	biological_process	defense response to Gram-positive bacterium
F	0050868	biological_process	negative regulation of T cell activation
F	0051604	biological_process	protein maturation
F	0070062	cellular_component	extracellular exosome
F	0071222	biological_process	cellular response to lipopolysaccharide
F	0089720	molecular_function	caspase binding
F	0098786	biological_process	biofilm matrix disassembly
F	1901731	biological_process	positive regulation of platelet aggregation
H	0000122	biological_process	negative regulation of transcription by RNA polymerase II
H	0002020	molecular_function	protease binding
H	0002438	biological_process	acute inflammatory response to antigenic stimulus
H	0002523	biological_process	leukocyte migration involved in inflammatory response
H	0002812	biological_process	biosynthetic process of antibacterial peptides active against Gram-negative bacteria
H	0003714	molecular_function	transcription corepressor activity
H	0004175	molecular_function	endopeptidase activity
H	0004252	molecular_function	serine-type endopeptidase activity
H	0005515	molecular_function	protein binding
H	0005576	cellular_component	extracellular region
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006508	biological_process	proteolysis
H	0006874	biological_process	intracellular calcium ion homeostasis
H	0006909	biological_process	phagocytosis
H	0008201	molecular_function	heparin binding
H	0008233	molecular_function	peptidase activity
H	0008236	molecular_function	serine-type peptidase activity
H	0009411	biological_process	response to UV
H	0009986	cellular_component	cell surface
H	0017053	cellular_component	transcription repressor complex
H	0019955	molecular_function	cytokine binding
H	0022617	biological_process	extracellular matrix disassembly
H	0030141	cellular_component	secretory granule
H	0030163	biological_process	protein catabolic process
H	0031012	cellular_component	extracellular matrix
H	0031452	biological_process	negative regulation of heterochromatin formation
H	0032682	biological_process	negative regulation of chemokine production
H	0032717	biological_process	negative regulation of interleukin-8 production
H	0032757	biological_process	positive regulation of interleukin-8 production
H	0035578	cellular_component	azurophil granule lumen
H	0035580	cellular_component	specific granule lumen
H	0042582	cellular_component	azurophil granule
H	0042742	biological_process	defense response to bacterium
H	0043406	biological_process	positive regulation of MAP kinase activity
H	0045335	cellular_component	phagocytic vesicle
H	0048661	biological_process	positive regulation of smooth muscle cell proliferation
H	0050728	biological_process	negative regulation of inflammatory response
H	0050922	biological_process	negative regulation of chemotaxis
H	0070062	cellular_component	extracellular exosome
H	0070269	biological_process	pyroptotic inflammatory response
H	0070943	biological_process	neutrophil-mediated killing of symbiont cell
H	0070944	biological_process	neutrophil-mediated killing of bacterium
H	0070945	biological_process	neutrophil-mediated killing of gram-negative bacterium
H	0070947	biological_process	neutrophil-mediated killing of fungus
H	0140644	cellular_component	neutrophil extracellular trap
H	0140645	biological_process	neutrophil extracellular trap formation
H	1903238	biological_process	positive regulation of leukocyte tethering or rolling
I	0002003	biological_process	angiotensin maturation
I	0002548	biological_process	monocyte chemotaxis
I	0004252	molecular_function	serine-type endopeptidase activity
I	0005515	molecular_function	protein binding
I	0005576	cellular_component	extracellular region
I	0005634	cellular_component	nucleus
I	0005764	cellular_component	lysosome
I	0005829	cellular_component	cytosol
I	0005886	cellular_component	plasma membrane
I	0006508	biological_process	proteolysis
I	0006955	biological_process	immune response
I	0008201	molecular_function	heparin binding
I	0008233	molecular_function	peptidase activity
I	0008236	molecular_function	serine-type peptidase activity
I	0010494	cellular_component	cytoplasmic stress granule
I	0016020	cellular_component	membrane
I	0016485	biological_process	protein processing
I	0019221	biological_process	cytokine-mediated signaling pathway
I	0019731	biological_process	antibacterial humoral response
I	0022617	biological_process	extracellular matrix disassembly
I	0030141	cellular_component	secretory granule
I	0030168	biological_process	platelet activation
I	0031012	cellular_component	extracellular matrix
I	0035578	cellular_component	azurophil granule lumen
I	0035590	biological_process	purinergic nucleotide receptor signaling pathway
I	0042119	biological_process	neutrophil activation
I	0048018	molecular_function	receptor ligand activity
I	0050829	biological_process	defense response to Gram-negative bacterium
I	0050830	biological_process	defense response to Gram-positive bacterium
I	0050868	biological_process	negative regulation of T cell activation
I	0051604	biological_process	protein maturation
I	0070062	cellular_component	extracellular exosome
I	0071222	biological_process	cellular response to lipopolysaccharide
I	0089720	molecular_function	caspase binding
I	0098786	biological_process	biofilm matrix disassembly
I	1901731	biological_process	positive regulation of platelet aggregation

Functional Information from PROSITE/UniProt

site_id	PS00134
Number of Residues	6
Details	TRYPSIN_HIS Serine proteases, trypsin family, histidine active site. LTAAHC

Chain	Residue	Details
A	LEU55-CYS60
B	MET65-CYS70

---

site_id	PS00135
Number of Residues	12
Details	TRYPSIN_SER Serine proteases, trypsin family, serine active site. AAfkGDSGGPLL

Chain	Residue	Details
A	ALA190-LEU201
B	GLY195-VAL206

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1317
Details	Domain: {"description":"Peptidase S1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00274","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	54
Details	Region: {"description":"Important for antimicrobial activity","evidences":[{"source":"PubMed","id":"2116408","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	9
Details	Active site: {"description":"Charge relay system","evidences":[{"source":"PROSITE-ProRule","id":"PRU00274","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	Glycosylation: {"description":"N-linked (GlcNAc...) (paucimannose) asparagine; alternate","evidences":[{"source":"PubMed","id":"26274980","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	9
Details	Active site: {"description":"Charge relay system","evidences":[{"source":"PubMed","id":"2911584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	3
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	3
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"26274980","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"3550808","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	3
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"3550808","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---