8FVW

CryoEM structure of E.coli transcription elongation complex bound to ppGpp

Functional Information from GO Data

Chain	GOid	namespace	contents
D	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
D	0000428	cellular_component	DNA-directed RNA polymerase complex
D	0003677	molecular_function	DNA binding
D	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006351	biological_process	DNA-templated transcription
D	0006352	biological_process	DNA-templated transcription initiation
D	0006879	biological_process	intracellular iron ion homeostasis
D	0008023	cellular_component	transcription elongation factor complex
D	0009408	biological_process	response to heat
D	0016020	cellular_component	membrane
D	0016779	molecular_function	nucleotidyltransferase activity
D	0031564	biological_process	transcription antitermination
D	0032784	biological_process	regulation of DNA-templated transcription elongation
D	0034062	molecular_function	5'-3' RNA polymerase activity
D	0036460	biological_process	cellular response to cell envelope stress
D	0042128	biological_process	nitrate assimilation
D	0044780	biological_process	bacterial-type flagellum assembly
D	0046983	molecular_function	protein dimerization activity
D	0048870	biological_process	cell motility
D	0071973	biological_process	bacterial-type flagellum-dependent cell motility
D	0090605	biological_process	submerged biofilm formation
D	2000142	biological_process	regulation of DNA-templated transcription initiation
E	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
E	0000428	cellular_component	DNA-directed RNA polymerase complex
E	0003677	molecular_function	DNA binding
E	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006351	biological_process	DNA-templated transcription
E	0006352	biological_process	DNA-templated transcription initiation
E	0006879	biological_process	intracellular iron ion homeostasis
E	0008023	cellular_component	transcription elongation factor complex
E	0009408	biological_process	response to heat
E	0016020	cellular_component	membrane
E	0016779	molecular_function	nucleotidyltransferase activity
E	0031564	biological_process	transcription antitermination
E	0032784	biological_process	regulation of DNA-templated transcription elongation
E	0034062	molecular_function	5'-3' RNA polymerase activity
E	0036460	biological_process	cellular response to cell envelope stress
E	0042128	biological_process	nitrate assimilation
E	0044780	biological_process	bacterial-type flagellum assembly
E	0046983	molecular_function	protein dimerization activity
E	0048870	biological_process	cell motility
E	0071973	biological_process	bacterial-type flagellum-dependent cell motility
E	0090605	biological_process	submerged biofilm formation
E	2000142	biological_process	regulation of DNA-templated transcription initiation
F	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
F	0000428	cellular_component	DNA-directed RNA polymerase complex
F	0003677	molecular_function	DNA binding
F	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006351	biological_process	DNA-templated transcription
F	0006352	biological_process	DNA-templated transcription initiation
F	0006879	biological_process	intracellular iron ion homeostasis
F	0008023	cellular_component	transcription elongation factor complex
F	0009408	biological_process	response to heat
F	0016020	cellular_component	membrane
F	0016779	molecular_function	nucleotidyltransferase activity
F	0031564	biological_process	transcription antitermination
F	0032549	molecular_function	ribonucleoside binding
F	0032784	biological_process	regulation of DNA-templated transcription elongation
F	0034062	molecular_function	5'-3' RNA polymerase activity
F	0036460	biological_process	cellular response to cell envelope stress
F	0042128	biological_process	nitrate assimilation
F	0044780	biological_process	bacterial-type flagellum assembly
F	0046677	biological_process	response to antibiotic
F	0048870	biological_process	cell motility
F	0071973	biological_process	bacterial-type flagellum-dependent cell motility
F	0090605	biological_process	submerged biofilm formation
F	2000142	biological_process	regulation of DNA-templated transcription initiation
G	0000287	molecular_function	magnesium ion binding
G	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
G	0000428	cellular_component	DNA-directed RNA polymerase complex
G	0003677	molecular_function	DNA binding
G	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006351	biological_process	DNA-templated transcription
G	0006352	biological_process	DNA-templated transcription initiation
G	0006879	biological_process	intracellular iron ion homeostasis
G	0008023	cellular_component	transcription elongation factor complex
G	0008270	molecular_function	zinc ion binding
G	0009408	biological_process	response to heat
G	0016020	cellular_component	membrane
G	0016779	molecular_function	nucleotidyltransferase activity
G	0031564	biological_process	transcription antitermination
G	0032784	biological_process	regulation of DNA-templated transcription elongation
G	0034062	molecular_function	5'-3' RNA polymerase activity
G	0036460	biological_process	cellular response to cell envelope stress
G	0042128	biological_process	nitrate assimilation
G	0044780	biological_process	bacterial-type flagellum assembly
G	0046677	biological_process	response to antibiotic
G	0046872	molecular_function	metal ion binding
G	0048870	biological_process	cell motility
G	0071973	biological_process	bacterial-type flagellum-dependent cell motility
G	0090605	biological_process	submerged biofilm formation
G	2000142	biological_process	regulation of DNA-templated transcription initiation
H	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
H	0000428	cellular_component	DNA-directed RNA polymerase complex
H	0003677	molecular_function	DNA binding
H	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
H	0005829	cellular_component	cytosol
H	0006351	biological_process	DNA-templated transcription
H	0006352	biological_process	DNA-templated transcription initiation
H	0006879	biological_process	intracellular iron ion homeostasis
H	0008023	cellular_component	transcription elongation factor complex
H	0009408	biological_process	response to heat
H	0016779	molecular_function	nucleotidyltransferase activity
H	0030880	cellular_component	RNA polymerase complex
H	0031564	biological_process	transcription antitermination
H	0032784	biological_process	regulation of DNA-templated transcription elongation
H	0034062	molecular_function	5'-3' RNA polymerase activity
H	0036460	biological_process	cellular response to cell envelope stress
H	0042128	biological_process	nitrate assimilation
H	0044780	biological_process	bacterial-type flagellum assembly
H	0048870	biological_process	cell motility
H	0065003	biological_process	protein-containing complex assembly
H	0071973	biological_process	bacterial-type flagellum-dependent cell motility
H	0090605	biological_process	submerged biofilm formation
H	2000142	biological_process	regulation of DNA-templated transcription initiation

Functional Information from PROSITE/UniProt

site_id	PS01166
Number of Residues	13
Details	RNA_POL_BETA RNA polymerases beta chain signature. GdKMAGrHGNKGV

Chain	Residue	Details
F	GLY1063-VAL1075

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	11
Details	BINDING: BINDING => ECO:0000269|PubMed:32871103, ECO:0007744|PDB:4MEX, ECO:0007744|PDB:4MEY

Chain	Residue	Details
G	CYS70
G	CYS895
G	CYS898
G	CYS72
G	CYS85
G	CYS88
G	ASP460
G	ASP462
G	ASP464
G	CYS814
G	CYS888

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site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000255|HAMAP-Rule:MF_01322, ECO:0000269|PubMed:18723842

Chain	Residue	Details
G	LYS983
E	LYS297

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site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: N6-acetyllysine; by PatZ => ECO:0000269|PubMed:21696463

Chain	Residue	Details
D	LYS298
E	LYS298

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