Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8F8Q

Cryo-EM structure of the CapZ-capped barbed end of F-actin

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0001725	cellular_component	stress fiber
A	0003785	molecular_function	actin monomer binding
A	0005509	molecular_function	calcium ion binding
A	0005515	molecular_function	protein binding
A	0005523	molecular_function	tropomyosin binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005856	cellular_component	cytoskeleton
A	0005865	cellular_component	striated muscle thin filament
A	0005884	cellular_component	actin filament
A	0010628	biological_process	positive regulation of gene expression
A	0016787	molecular_function	hydrolase activity
A	0019904	molecular_function	protein domain specific binding
A	0030027	cellular_component	lamellipodium
A	0030041	biological_process	actin filament polymerization
A	0030175	cellular_component	filopodium
A	0030240	biological_process	skeletal muscle thin filament assembly
A	0031013	molecular_function	troponin I binding
A	0031432	molecular_function	titin binding
A	0031941	cellular_component	filamentous actin
A	0032036	molecular_function	myosin heavy chain binding
A	0032432	cellular_component	actin filament bundle
A	0042802	molecular_function	identical protein binding
A	0044297	cellular_component	cell body
A	0048306	molecular_function	calcium-dependent protein binding
A	0048741	biological_process	skeletal muscle fiber development
A	0051017	biological_process	actin filament bundle assembly
A	0090131	biological_process	mesenchyme migration
A	0098723	cellular_component	skeletal muscle myofibril
A	0140660	molecular_function	cytoskeletal motor activator activity
B	0000287	molecular_function	magnesium ion binding
B	0001725	cellular_component	stress fiber
B	0003785	molecular_function	actin monomer binding
B	0005509	molecular_function	calcium ion binding
B	0005515	molecular_function	protein binding
B	0005523	molecular_function	tropomyosin binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005856	cellular_component	cytoskeleton
B	0005865	cellular_component	striated muscle thin filament
B	0005884	cellular_component	actin filament
B	0010628	biological_process	positive regulation of gene expression
B	0016787	molecular_function	hydrolase activity
B	0019904	molecular_function	protein domain specific binding
B	0030027	cellular_component	lamellipodium
B	0030041	biological_process	actin filament polymerization
B	0030175	cellular_component	filopodium
B	0030240	biological_process	skeletal muscle thin filament assembly
B	0031013	molecular_function	troponin I binding
B	0031432	molecular_function	titin binding
B	0031941	cellular_component	filamentous actin
B	0032036	molecular_function	myosin heavy chain binding
B	0032432	cellular_component	actin filament bundle
B	0042802	molecular_function	identical protein binding
B	0044297	cellular_component	cell body
B	0048306	molecular_function	calcium-dependent protein binding
B	0048741	biological_process	skeletal muscle fiber development
B	0051017	biological_process	actin filament bundle assembly
B	0090131	biological_process	mesenchyme migration
B	0098723	cellular_component	skeletal muscle myofibril
B	0140660	molecular_function	cytoskeletal motor activator activity
C	0000287	molecular_function	magnesium ion binding
C	0001725	cellular_component	stress fiber
C	0003785	molecular_function	actin monomer binding
C	0005509	molecular_function	calcium ion binding
C	0005515	molecular_function	protein binding
C	0005523	molecular_function	tropomyosin binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005856	cellular_component	cytoskeleton
C	0005865	cellular_component	striated muscle thin filament
C	0005884	cellular_component	actin filament
C	0010628	biological_process	positive regulation of gene expression
C	0016787	molecular_function	hydrolase activity
C	0019904	molecular_function	protein domain specific binding
C	0030027	cellular_component	lamellipodium
C	0030041	biological_process	actin filament polymerization
C	0030175	cellular_component	filopodium
C	0030240	biological_process	skeletal muscle thin filament assembly
C	0031013	molecular_function	troponin I binding
C	0031432	molecular_function	titin binding
C	0031941	cellular_component	filamentous actin
C	0032036	molecular_function	myosin heavy chain binding
C	0032432	cellular_component	actin filament bundle
C	0042802	molecular_function	identical protein binding
C	0044297	cellular_component	cell body
C	0048306	molecular_function	calcium-dependent protein binding
C	0048741	biological_process	skeletal muscle fiber development
C	0051017	biological_process	actin filament bundle assembly
C	0090131	biological_process	mesenchyme migration
C	0098723	cellular_component	skeletal muscle myofibril
C	0140660	molecular_function	cytoskeletal motor activator activity
D	0000287	molecular_function	magnesium ion binding
D	0001725	cellular_component	stress fiber
D	0003785	molecular_function	actin monomer binding
D	0005509	molecular_function	calcium ion binding
D	0005515	molecular_function	protein binding
D	0005523	molecular_function	tropomyosin binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005856	cellular_component	cytoskeleton
D	0005865	cellular_component	striated muscle thin filament
D	0005884	cellular_component	actin filament
D	0010628	biological_process	positive regulation of gene expression
D	0016787	molecular_function	hydrolase activity
D	0019904	molecular_function	protein domain specific binding
D	0030027	cellular_component	lamellipodium
D	0030041	biological_process	actin filament polymerization
D	0030175	cellular_component	filopodium
D	0030240	biological_process	skeletal muscle thin filament assembly
D	0031013	molecular_function	troponin I binding
D	0031432	molecular_function	titin binding
D	0031941	cellular_component	filamentous actin
D	0032036	molecular_function	myosin heavy chain binding
D	0032432	cellular_component	actin filament bundle
D	0042802	molecular_function	identical protein binding
D	0044297	cellular_component	cell body
D	0048306	molecular_function	calcium-dependent protein binding
D	0048741	biological_process	skeletal muscle fiber development
D	0051017	biological_process	actin filament bundle assembly
D	0090131	biological_process	mesenchyme migration
D	0098723	cellular_component	skeletal muscle myofibril
D	0140660	molecular_function	cytoskeletal motor activator activity
E	0000287	molecular_function	magnesium ion binding
E	0001725	cellular_component	stress fiber
E	0003785	molecular_function	actin monomer binding
E	0005509	molecular_function	calcium ion binding
E	0005515	molecular_function	protein binding
E	0005523	molecular_function	tropomyosin binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005856	cellular_component	cytoskeleton
E	0005865	cellular_component	striated muscle thin filament
E	0005884	cellular_component	actin filament
E	0010628	biological_process	positive regulation of gene expression
E	0016787	molecular_function	hydrolase activity
E	0019904	molecular_function	protein domain specific binding
E	0030027	cellular_component	lamellipodium
E	0030041	biological_process	actin filament polymerization
E	0030175	cellular_component	filopodium
E	0030240	biological_process	skeletal muscle thin filament assembly
E	0031013	molecular_function	troponin I binding
E	0031432	molecular_function	titin binding
E	0031941	cellular_component	filamentous actin
E	0032036	molecular_function	myosin heavy chain binding
E	0032432	cellular_component	actin filament bundle
E	0042802	molecular_function	identical protein binding
E	0044297	cellular_component	cell body
E	0048306	molecular_function	calcium-dependent protein binding
E	0048741	biological_process	skeletal muscle fiber development
E	0051017	biological_process	actin filament bundle assembly
E	0090131	biological_process	mesenchyme migration
E	0098723	cellular_component	skeletal muscle myofibril
E	0140660	molecular_function	cytoskeletal motor activator activity
F	0000287	molecular_function	magnesium ion binding
F	0001725	cellular_component	stress fiber
F	0003785	molecular_function	actin monomer binding
F	0005509	molecular_function	calcium ion binding
F	0005515	molecular_function	protein binding
F	0005523	molecular_function	tropomyosin binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005856	cellular_component	cytoskeleton
F	0005865	cellular_component	striated muscle thin filament
F	0005884	cellular_component	actin filament
F	0010628	biological_process	positive regulation of gene expression
F	0016787	molecular_function	hydrolase activity
F	0019904	molecular_function	protein domain specific binding
F	0030027	cellular_component	lamellipodium
F	0030041	biological_process	actin filament polymerization
F	0030175	cellular_component	filopodium
F	0030240	biological_process	skeletal muscle thin filament assembly
F	0031013	molecular_function	troponin I binding
F	0031432	molecular_function	titin binding
F	0031941	cellular_component	filamentous actin
F	0032036	molecular_function	myosin heavy chain binding
F	0032432	cellular_component	actin filament bundle
F	0042802	molecular_function	identical protein binding
F	0044297	cellular_component	cell body
F	0048306	molecular_function	calcium-dependent protein binding
F	0048741	biological_process	skeletal muscle fiber development
F	0051017	biological_process	actin filament bundle assembly
F	0090131	biological_process	mesenchyme migration
F	0098723	cellular_component	skeletal muscle myofibril
F	0140660	molecular_function	cytoskeletal motor activator activity
G	0003779	molecular_function	actin binding
G	0005515	molecular_function	protein binding
G	0005576	cellular_component	extracellular region
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005856	cellular_component	cytoskeleton
G	0008290	cellular_component	F-actin capping protein complex
G	0015629	cellular_component	actin cytoskeleton
G	0030036	biological_process	actin cytoskeleton organization
G	0030863	cellular_component	cortical cytoskeleton
G	0034329	biological_process	cell junction assembly
G	0045296	molecular_function	cadherin binding
G	0051015	molecular_function	actin filament binding
G	0051016	biological_process	barbed-end actin filament capping
G	0051693	biological_process	actin filament capping
G	0065003	biological_process	protein-containing complex assembly
G	0070062	cellular_component	extracellular exosome
G	0071203	cellular_component	WASH complex
H	0000902	biological_process	cell morphogenesis
H	0003779	molecular_function	actin binding
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005856	cellular_component	cytoskeleton
H	0005903	cellular_component	brush border
H	0007010	biological_process	cytoskeleton organization
H	0008154	biological_process	actin polymerization or depolymerization
H	0008290	cellular_component	F-actin capping protein complex
H	0010591	biological_process	regulation of lamellipodium assembly
H	0014069	cellular_component	postsynaptic density
H	0015629	cellular_component	actin cytoskeleton
H	0016020	cellular_component	membrane
H	0022604	biological_process	regulation of cell morphogenesis
H	0030017	cellular_component	sarcomere
H	0030027	cellular_component	lamellipodium
H	0030030	biological_process	cell projection organization
H	0030032	biological_process	lamellipodium assembly
H	0030036	biological_process	actin cytoskeleton organization
H	0030863	cellular_component	cortical cytoskeleton
H	0032279	cellular_component	asymmetric synapse
H	0045296	molecular_function	cadherin binding
H	0051015	molecular_function	actin filament binding
H	0051016	biological_process	barbed-end actin filament capping
H	0051490	biological_process	negative regulation of filopodium assembly
H	0051693	biological_process	actin filament capping
H	0070062	cellular_component	extracellular exosome
H	0071203	cellular_component	WASH complex
H	0098685	cellular_component	Schaffer collateral - CA1 synapse
H	0098686	cellular_component	hippocampal mossy fiber to CA3 synapse
H	0120212	cellular_component	sperm head-tail coupling apparatus

Functional Information from PROSITE/UniProt

site_id	PS00231
Number of Residues	6
Details	F_ACTIN_CAPPING_BETA F-actin capping protein beta subunit signature. CDYNRD

Chain	Residue	Details
H	CYS62-ASP67

site_id	PS00406
Number of Residues	11
Details	ACTINS_1 Actins signature 1. YVGDEAQs.KRG

Chain	Residue	Details
A	TYR53-GLY63

site_id	PS00432
Number of Residues	9
Details	ACTINS_2 Actins signature 2. WITKqEYDE

Chain	Residue	Details
A	TRP356-GLU364

site_id	PS00748
Number of Residues	9
Details	F_ACTIN_CAPPING_A_1 F-actin capping protein alpha subunit signature 1. VHYYEDGNV

Chain	Residue	Details
G	VAL196-VAL204

site_id	PS00749
Number of Residues	11
Details	F_ACTIN_CAPPING_A_2 F-actin capping protein alpha subunit signature 2. KaLRRqLPVTR

Chain	Residue	Details
G	LYS256-ARG266

site_id	PS01132
Number of Residues	13
Details	ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR

Chain	Residue	Details
A	LEU104-ARG116

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
H	SER2
B	CYS0
C	CYS0
D	CYS0
E	CYS0
F	CYS0

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0007744\|PubMed:19608861

Chain	Residue	Details
H	LYS235
B	ASP1
C	ASP1
D	ASP1
E	ASP1
F	ASP1

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0007744\|PubMed:19608861

Chain	Residue	Details
G	LYS19
E	MET47
F	MET44
F	MET47
G	LYS97
B	MET44
B	MET47
C	MET44
C	MET47
D	MET44
D	MET47
E	MET44

site_id	SWS_FT_FI4
Number of Residues	6
Details	MOD_RES: N6-malonyllysine => ECO:0000250

Chain	Residue	Details
A	LYS61
B	LYS61
C	LYS61
D	LYS61
E	LYS61
F	LYS61

site_id	SWS_FT_FI5
Number of Residues	6
Details	MOD_RES: Tele-methylhistidine => ECO:0000269\|PubMed:1150665, ECO:0000269\|PubMed:16905096, ECO:0000269\|PubMed:213279, ECO:0000269\|PubMed:2395459, ECO:0000269\|PubMed:499690, ECO:0007744\|PDB:1ATN, ECO:0007744\|PDB:1NWK

Chain	Residue	Details
A	HIC73
B	HIC73
C	HIC73
D	HIC73
E	HIC73
F	HIC73

site_id	SWS_FT_FI6
Number of Residues	6
Details	MOD_RES: N6-methyllysine => ECO:0000250\|UniProtKB:P68133

Chain	Residue	Details
A	LYS84
B	LYS84
C	LYS84
D	LYS84
E	LYS84
F	LYS84

site_id	SWS_FT_FI7
Number of Residues	6
Details	MOD_RES: ADP-ribosylarginine; by SpvB => ECO:0000305\|PubMed:16905096

Chain	Residue	Details
A	ARG177
B	ARG177
C	ARG177
D	ARG177
E	ARG177
F	ARG177

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)