Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8EU4

Escherichia coli pyruvate kinase A301S

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003824	molecular_function	catalytic activity
A	0004743	molecular_function	pyruvate kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006096	biological_process	glycolytic process
A	0009408	biological_process	response to heat
A	0016020	cellular_component	membrane
A	0016301	molecular_function	kinase activity
A	0030955	molecular_function	potassium ion binding
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
A	1902912	cellular_component	pyruvate kinase complex
B	0000287	molecular_function	magnesium ion binding
B	0003824	molecular_function	catalytic activity
B	0004743	molecular_function	pyruvate kinase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006096	biological_process	glycolytic process
B	0009408	biological_process	response to heat
B	0016020	cellular_component	membrane
B	0016301	molecular_function	kinase activity
B	0030955	molecular_function	potassium ion binding
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding
B	1902912	cellular_component	pyruvate kinase complex
C	0000287	molecular_function	magnesium ion binding
C	0003824	molecular_function	catalytic activity
C	0004743	molecular_function	pyruvate kinase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006096	biological_process	glycolytic process
C	0009408	biological_process	response to heat
C	0016020	cellular_component	membrane
C	0016301	molecular_function	kinase activity
C	0030955	molecular_function	potassium ion binding
C	0042802	molecular_function	identical protein binding
C	0046872	molecular_function	metal ion binding
C	1902912	cellular_component	pyruvate kinase complex
D	0000287	molecular_function	magnesium ion binding
D	0003824	molecular_function	catalytic activity
D	0004743	molecular_function	pyruvate kinase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006096	biological_process	glycolytic process
D	0009408	biological_process	response to heat
D	0016020	cellular_component	membrane
D	0016301	molecular_function	kinase activity
D	0030955	molecular_function	potassium ion binding
D	0042802	molecular_function	identical protein binding
D	0046872	molecular_function	metal ion binding
D	1902912	cellular_component	pyruvate kinase complex
E	0000287	molecular_function	magnesium ion binding
E	0003824	molecular_function	catalytic activity
E	0004743	molecular_function	pyruvate kinase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006096	biological_process	glycolytic process
E	0009408	biological_process	response to heat
E	0016020	cellular_component	membrane
E	0016301	molecular_function	kinase activity
E	0030955	molecular_function	potassium ion binding
E	0042802	molecular_function	identical protein binding
E	0046872	molecular_function	metal ion binding
E	1902912	cellular_component	pyruvate kinase complex
F	0000287	molecular_function	magnesium ion binding
F	0003824	molecular_function	catalytic activity
F	0004743	molecular_function	pyruvate kinase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006096	biological_process	glycolytic process
F	0009408	biological_process	response to heat
F	0016020	cellular_component	membrane
F	0016301	molecular_function	kinase activity
F	0030955	molecular_function	potassium ion binding
F	0042802	molecular_function	identical protein binding
F	0046872	molecular_function	metal ion binding
F	1902912	cellular_component	pyruvate kinase complex
G	0000287	molecular_function	magnesium ion binding
G	0003824	molecular_function	catalytic activity
G	0004743	molecular_function	pyruvate kinase activity
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006096	biological_process	glycolytic process
G	0009408	biological_process	response to heat
G	0016020	cellular_component	membrane
G	0016301	molecular_function	kinase activity
G	0030955	molecular_function	potassium ion binding
G	0042802	molecular_function	identical protein binding
G	0046872	molecular_function	metal ion binding
G	1902912	cellular_component	pyruvate kinase complex
H	0000287	molecular_function	magnesium ion binding
H	0003824	molecular_function	catalytic activity
H	0004743	molecular_function	pyruvate kinase activity
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006096	biological_process	glycolytic process
H	0009408	biological_process	response to heat
H	0016020	cellular_component	membrane
H	0016301	molecular_function	kinase activity
H	0030955	molecular_function	potassium ion binding
H	0042802	molecular_function	identical protein binding
H	0046872	molecular_function	metal ion binding
H	1902912	cellular_component	pyruvate kinase complex

Functional Information from PROSITE/UniProt

site_id	PS00110
Number of Residues	13
Details	PYRUVATE_KINASE Pyruvate kinase active site signature. IhIISKIENqEGL

Chain	Residue	Details
A	ILE215-LEU227

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	80
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P30613

Chain	Residue	Details
A	ARG32
A	THR278
E	ARG32
E	ASN34
E	SER36
E	ASP66
E	THR67
E	LYS220
E	GLU222
E	GLY245
E	ASP246
A	ASN34
E	THR278
D	ARG32
D	ASN34
D	SER36
D	ASP66
D	THR67
D	LYS220
D	GLU222
D	GLY245
D	ASP246
A	SER36
D	THR278
G	ARG32
G	ASN34
G	SER36
G	ASP66
G	THR67
G	LYS220
G	GLU222
G	GLY245
G	ASP246
A	ASP66
G	THR278
B	ARG32
B	ASN34
B	SER36
B	ASP66
B	THR67
B	LYS220
B	GLU222
B	GLY245
B	ASP246
A	THR67
B	THR278
F	ARG32
F	ASN34
F	SER36
F	ASP66
F	THR67
F	LYS220
F	GLU222
F	GLY245
F	ASP246
A	LYS220
F	THR278
H	ARG32
H	ASN34
H	SER36
H	ASP66
H	THR67
H	LYS220
H	GLU222
H	GLY245
H	ASP246
A	GLU222
H	THR278
C	ARG32
C	ASN34
C	SER36
C	ASP66
C	THR67
C	LYS220
C	GLU222
C	GLY245
C	ASP246
A	GLY245
C	THR278
A	ASP246

site_id	SWS_FT_FI2
Number of Residues	16
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P14618

Chain	Residue	Details
A	ARG73
B	LYS156
F	ARG73
F	LYS156
H	ARG73
H	LYS156
C	ARG73
C	LYS156
A	LYS156
E	ARG73
E	LYS156
D	ARG73
D	LYS156
G	ARG73
G	LYS156
B	ARG73

site_id	SWS_FT_FI3
Number of Residues	8
Details	SITE: Transition state stabilizer => ECO:0000250\|UniProtKB:P00549

Chain	Residue	Details
A	LYS220
E	LYS220
D	LYS220
G	LYS220
B	LYS220
F	LYS220
H	LYS220
C	LYS220

site_id	SWS_FT_FI4
Number of Residues	16
Details	MOD_RES: N6-acetyllysine => ECO:0000269\|PubMed:18723842

Chain	Residue	Details
A	LYS76
B	LYS319
F	LYS76
F	LYS319
H	LYS76
H	LYS319
C	LYS76
C	LYS319
A	LYS319
E	LYS76
E	LYS319
D	LYS76
D	LYS319
G	LYS76
G	LYS319
B	LYS76

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)