8ESI

Bile Salt Hydrolase from B. longum with covalent inhibitor bound

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005515	molecular_function	protein binding
A	0006629	biological_process	lipid metabolic process
A	0006699	biological_process	bile acid biosynthetic process
A	0009274	cellular_component	peptidoglycan-based cell wall
A	0016740	molecular_function	transferase activity
A	0016787	molecular_function	hydrolase activity
A	0045302	molecular_function	choloylglycine hydrolase activity
A	0047742	molecular_function	chenodeoxycholoyltaurine hydrolase activity
B	0005515	molecular_function	protein binding
B	0006629	biological_process	lipid metabolic process
B	0006699	biological_process	bile acid biosynthetic process
B	0009274	cellular_component	peptidoglycan-based cell wall
B	0016740	molecular_function	transferase activity
B	0016787	molecular_function	hydrolase activity
B	0045302	molecular_function	choloylglycine hydrolase activity
B	0047742	molecular_function	chenodeoxycholoyltaurine hydrolase activity
C	0005515	molecular_function	protein binding
C	0006629	biological_process	lipid metabolic process
C	0006699	biological_process	bile acid biosynthetic process
C	0009274	cellular_component	peptidoglycan-based cell wall
C	0016740	molecular_function	transferase activity
C	0016787	molecular_function	hydrolase activity
C	0045302	molecular_function	choloylglycine hydrolase activity
C	0047742	molecular_function	chenodeoxycholoyltaurine hydrolase activity
D	0005515	molecular_function	protein binding
D	0006629	biological_process	lipid metabolic process
D	0006699	biological_process	bile acid biosynthetic process
D	0009274	cellular_component	peptidoglycan-based cell wall
D	0016740	molecular_function	transferase activity
D	0016787	molecular_function	hydrolase activity
D	0045302	molecular_function	choloylglycine hydrolase activity
D	0047742	molecular_function	chenodeoxycholoyltaurine hydrolase activity
E	0005515	molecular_function	protein binding
E	0006629	biological_process	lipid metabolic process
E	0006699	biological_process	bile acid biosynthetic process
E	0009274	cellular_component	peptidoglycan-based cell wall
E	0016740	molecular_function	transferase activity
E	0016787	molecular_function	hydrolase activity
E	0045302	molecular_function	choloylglycine hydrolase activity
E	0047742	molecular_function	chenodeoxycholoyltaurine hydrolase activity
F	0005515	molecular_function	protein binding
F	0006629	biological_process	lipid metabolic process
F	0006699	biological_process	bile acid biosynthetic process
F	0009274	cellular_component	peptidoglycan-based cell wall
F	0016740	molecular_function	transferase activity
F	0016787	molecular_function	hydrolase activity
F	0045302	molecular_function	choloylglycine hydrolase activity
F	0047742	molecular_function	chenodeoxycholoyltaurine hydrolase activity
G	0005515	molecular_function	protein binding
G	0006629	biological_process	lipid metabolic process
G	0006699	biological_process	bile acid biosynthetic process
G	0009274	cellular_component	peptidoglycan-based cell wall
G	0016740	molecular_function	transferase activity
G	0016787	molecular_function	hydrolase activity
G	0045302	molecular_function	choloylglycine hydrolase activity
G	0047742	molecular_function	chenodeoxycholoyltaurine hydrolase activity
H	0005515	molecular_function	protein binding
H	0006629	biological_process	lipid metabolic process
H	0006699	biological_process	bile acid biosynthetic process
H	0009274	cellular_component	peptidoglycan-based cell wall
H	0016740	molecular_function	transferase activity
H	0016787	molecular_function	hydrolase activity
H	0045302	molecular_function	choloylglycine hydrolase activity
H	0047742	molecular_function	chenodeoxycholoyltaurine hydrolase activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Nucleophile; acyl-thioester intermediate => ECO:0000305|PubMed:10831430

Chain	Residue	Details
A	CYS2
B	CYS2
C	CYS2
D	CYS2
E	CYS2
F	CYS2
G	CYS2
H	CYS2

---

site_id	SWS_FT_FI2
Number of Residues	24
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P54965

Chain	Residue	Details
A	CYS2
D	CYS2
D	ARG18
D	ASN82
E	CYS2
E	ARG18
E	ASN82
F	CYS2
F	ARG18
F	ASN82
G	CYS2
A	ARG18
G	ARG18
G	ASN82
H	CYS2
H	ARG18
H	ASN82
A	ASN82
B	CYS2
B	ARG18
B	ASN82
C	CYS2
C	ARG18
C	ASN82

---