8EQ0

Escherichia coli pyruvate kinase G381A

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003824	molecular_function	catalytic activity
A	0004743	molecular_function	pyruvate kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006096	biological_process	glycolytic process
A	0009408	biological_process	response to heat
A	0016020	cellular_component	membrane
A	0016301	molecular_function	kinase activity
A	0030955	molecular_function	potassium ion binding
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
A	1902912	cellular_component	pyruvate kinase complex
B	0000287	molecular_function	magnesium ion binding
B	0003824	molecular_function	catalytic activity
B	0004743	molecular_function	pyruvate kinase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006096	biological_process	glycolytic process
B	0009408	biological_process	response to heat
B	0016020	cellular_component	membrane
B	0016301	molecular_function	kinase activity
B	0030955	molecular_function	potassium ion binding
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding
B	1902912	cellular_component	pyruvate kinase complex

Functional Information from PROSITE/UniProt

site_id	PS00110
Number of Residues	13
Details	PYRUVATE_KINASE Pyruvate kinase active site signature. IhIISKIENqEGL

Chain	Residue	Details
B	ILE215-LEU227

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	20
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P30613

Chain	Residue	Details
B	ARG32
B	THR278
A	ARG32
A	ASN34
A	SER36
A	ASP66
A	THR67
A	LYS220
A	GLU222
A	GLY245
A	ASP246
B	ASN34
A	THR278
B	SER36
B	ASP66
B	THR67
B	LYS220
B	GLU222
B	GLY245
B	ASP246

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P14618

Chain	Residue	Details
B	ARG73
B	LYS156
A	ARG73
A	LYS156

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	SITE: Transition state stabilizer => ECO:0000250|UniProtKB:P00549

Chain	Residue	Details
B	LYS220
A	LYS220

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: N6-acetyllysine => ECO:0000269|PubMed:18723842

Chain	Residue	Details
B	LYS76
B	LYS319
A	LYS76
A	LYS319

---