Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8E9B

Cryo-EM structure of S. pombe Arp2/3 complex in the branch junction

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003779	molecular_function	actin binding
A	0005200	molecular_function	structural constituent of cytoskeleton
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005885	cellular_component	Arp2/3 protein complex
A	0006897	biological_process	endocytosis
A	0015629	cellular_component	actin cytoskeleton
A	0030041	biological_process	actin filament polymerization
A	0030479	cellular_component	actin cortical patch
A	0032153	cellular_component	cell division site
A	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
A	0044396	biological_process	actin cortical patch organization
A	0051015	molecular_function	actin filament binding
A	0051285	cellular_component	cell cortex of cell tip
A	0090135	biological_process	actin filament branching
B	0000147	biological_process	actin cortical patch assembly
B	0000166	molecular_function	nucleotide binding
B	0003779	molecular_function	actin binding
B	0005200	molecular_function	structural constituent of cytoskeleton
B	0005524	molecular_function	ATP binding
B	0005885	cellular_component	Arp2/3 protein complex
B	0005938	cellular_component	cell cortex
B	0006897	biological_process	endocytosis
B	0015629	cellular_component	actin cytoskeleton
B	0030479	cellular_component	actin cortical patch
B	0031097	cellular_component	medial cortex
B	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
B	0051015	molecular_function	actin filament binding
B	0051285	cellular_component	cell cortex of cell tip
B	0090135	biological_process	actin filament branching
C	0000147	biological_process	actin cortical patch assembly
C	0003779	molecular_function	actin binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005885	cellular_component	Arp2/3 protein complex
C	0006897	biological_process	endocytosis
C	0015629	cellular_component	actin cytoskeleton
C	0030036	biological_process	actin cytoskeleton organization
C	0030479	cellular_component	actin cortical patch
C	0030866	biological_process	cortical actin cytoskeleton organization
C	0032153	cellular_component	cell division site
C	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
C	0051015	molecular_function	actin filament binding
C	0051286	cellular_component	cell tip
C	0090135	biological_process	actin filament branching
D	0000147	biological_process	actin cortical patch assembly
D	0003779	molecular_function	actin binding
D	0005200	molecular_function	structural constituent of cytoskeleton
D	0005634	cellular_component	nucleus
D	0005829	cellular_component	cytosol
D	0005885	cellular_component	Arp2/3 protein complex
D	0006897	biological_process	endocytosis
D	0015629	cellular_component	actin cytoskeleton
D	0030041	biological_process	actin filament polymerization
D	0030479	cellular_component	actin cortical patch
D	0032153	cellular_component	cell division site
D	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
D	0051015	molecular_function	actin filament binding
D	0051286	cellular_component	cell tip
D	0090135	biological_process	actin filament branching
E	0000147	biological_process	actin cortical patch assembly
E	0003779	molecular_function	actin binding
E	0005634	cellular_component	nucleus
E	0005826	cellular_component	actomyosin contractile ring
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0005885	cellular_component	Arp2/3 protein complex
E	0006897	biological_process	endocytosis
E	0007163	biological_process	establishment or maintenance of cell polarity
E	0015629	cellular_component	actin cytoskeleton
E	0030479	cellular_component	actin cortical patch
E	0030833	biological_process	regulation of actin filament polymerization
E	0032153	cellular_component	cell division site
E	0032432	cellular_component	actin filament bundle
E	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
E	0044396	biological_process	actin cortical patch organization
E	0051015	molecular_function	actin filament binding
E	0051286	cellular_component	cell tip
E	0090135	biological_process	actin filament branching
F	0000147	biological_process	actin cortical patch assembly
F	0003779	molecular_function	actin binding
F	0005634	cellular_component	nucleus
F	0005829	cellular_component	cytosol
F	0005885	cellular_component	Arp2/3 protein complex
F	0006897	biological_process	endocytosis
F	0015629	cellular_component	actin cytoskeleton
F	0030041	biological_process	actin filament polymerization
F	0030479	cellular_component	actin cortical patch
F	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
F	0051015	molecular_function	actin filament binding
F	0090135	biological_process	actin filament branching
G	0000147	biological_process	actin cortical patch assembly
G	0003779	molecular_function	actin binding
G	0005634	cellular_component	nucleus
G	0005829	cellular_component	cytosol
G	0005885	cellular_component	Arp2/3 protein complex
G	0006897	biological_process	endocytosis
G	0015629	cellular_component	actin cytoskeleton
G	0030479	cellular_component	actin cortical patch
G	0030674	molecular_function	protein-macromolecule adaptor activity
G	0030833	biological_process	regulation of actin filament polymerization
G	0030863	cellular_component	cortical cytoskeleton
G	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
G	0043332	cellular_component	mating projection tip
G	0044396	biological_process	actin cortical patch organization
G	0051015	molecular_function	actin filament binding
G	0090135	biological_process	actin filament branching
H	0000166	molecular_function	nucleotide binding
H	0001725	cellular_component	stress fiber
H	0005200	molecular_function	structural constituent of cytoskeleton
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005856	cellular_component	cytoskeleton
H	0005865	cellular_component	striated muscle thin filament
H	0005884	cellular_component	actin filament
H	0015629	cellular_component	actin cytoskeleton
H	0016787	molecular_function	hydrolase activity
H	0030240	biological_process	skeletal muscle thin filament assembly
H	0048741	biological_process	skeletal muscle fiber development
I	0000166	molecular_function	nucleotide binding
I	0001725	cellular_component	stress fiber
I	0005200	molecular_function	structural constituent of cytoskeleton
I	0005515	molecular_function	protein binding
I	0005524	molecular_function	ATP binding
I	0005856	cellular_component	cytoskeleton
I	0005865	cellular_component	striated muscle thin filament
I	0005884	cellular_component	actin filament
I	0015629	cellular_component	actin cytoskeleton
I	0016787	molecular_function	hydrolase activity
I	0030240	biological_process	skeletal muscle thin filament assembly
I	0048741	biological_process	skeletal muscle fiber development
M	0000166	molecular_function	nucleotide binding
M	0001725	cellular_component	stress fiber
M	0005200	molecular_function	structural constituent of cytoskeleton
M	0005515	molecular_function	protein binding
M	0005524	molecular_function	ATP binding
M	0005856	cellular_component	cytoskeleton
M	0005865	cellular_component	striated muscle thin filament
M	0005884	cellular_component	actin filament
M	0015629	cellular_component	actin cytoskeleton
M	0016787	molecular_function	hydrolase activity
M	0030240	biological_process	skeletal muscle thin filament assembly
M	0048741	biological_process	skeletal muscle fiber development
N	0000166	molecular_function	nucleotide binding
N	0001725	cellular_component	stress fiber
N	0005200	molecular_function	structural constituent of cytoskeleton
N	0005515	molecular_function	protein binding
N	0005524	molecular_function	ATP binding
N	0005856	cellular_component	cytoskeleton
N	0005865	cellular_component	striated muscle thin filament
N	0005884	cellular_component	actin filament
N	0015629	cellular_component	actin cytoskeleton
N	0016787	molecular_function	hydrolase activity
N	0030240	biological_process	skeletal muscle thin filament assembly
N	0048741	biological_process	skeletal muscle fiber development
O	0000166	molecular_function	nucleotide binding
O	0001725	cellular_component	stress fiber
O	0005200	molecular_function	structural constituent of cytoskeleton
O	0005515	molecular_function	protein binding
O	0005524	molecular_function	ATP binding
O	0005856	cellular_component	cytoskeleton
O	0005865	cellular_component	striated muscle thin filament
O	0005884	cellular_component	actin filament
O	0015629	cellular_component	actin cytoskeleton
O	0016787	molecular_function	hydrolase activity
O	0030240	biological_process	skeletal muscle thin filament assembly
O	0048741	biological_process	skeletal muscle fiber development
P	0000166	molecular_function	nucleotide binding
P	0001725	cellular_component	stress fiber
P	0005200	molecular_function	structural constituent of cytoskeleton
P	0005515	molecular_function	protein binding
P	0005524	molecular_function	ATP binding
P	0005856	cellular_component	cytoskeleton
P	0005865	cellular_component	striated muscle thin filament
P	0005884	cellular_component	actin filament
P	0015629	cellular_component	actin cytoskeleton
P	0016787	molecular_function	hydrolase activity
P	0030240	biological_process	skeletal muscle thin filament assembly
P	0048741	biological_process	skeletal muscle fiber development
Q	0000166	molecular_function	nucleotide binding
Q	0001725	cellular_component	stress fiber
Q	0005200	molecular_function	structural constituent of cytoskeleton
Q	0005515	molecular_function	protein binding
Q	0005524	molecular_function	ATP binding
Q	0005856	cellular_component	cytoskeleton
Q	0005865	cellular_component	striated muscle thin filament
Q	0005884	cellular_component	actin filament
Q	0015629	cellular_component	actin cytoskeleton
Q	0016787	molecular_function	hydrolase activity
Q	0030240	biological_process	skeletal muscle thin filament assembly
Q	0048741	biological_process	skeletal muscle fiber development
R	0000166	molecular_function	nucleotide binding
R	0001725	cellular_component	stress fiber
R	0005200	molecular_function	structural constituent of cytoskeleton
R	0005515	molecular_function	protein binding
R	0005524	molecular_function	ATP binding
R	0005856	cellular_component	cytoskeleton
R	0005865	cellular_component	striated muscle thin filament
R	0005884	cellular_component	actin filament
R	0015629	cellular_component	actin cytoskeleton
R	0016787	molecular_function	hydrolase activity
R	0030240	biological_process	skeletal muscle thin filament assembly
R	0048741	biological_process	skeletal muscle fiber development

Functional Information from PROSITE/UniProt

site_id	PS00406
Number of Residues	11
Details	ACTINS_1 Actins signature 1. YVGDEAQs.KRG

Chain	Residue	Details
M	TYR53-GLY63

site_id	PS00432
Number of Residues	9
Details	ACTINS_2 Actins signature 2. WITKqEYDE

Chain	Residue	Details
M	TRP356-GLU364

site_id	PS01132
Number of Residues	13
Details	ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEPpMNPvaNR

Chain	Residue	Details
B	LEU104-ARG116
M	LEU104-ARG116
A	LEU127-ARG139

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	11
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	39
Details	Repeat: {"description":"WD 1"}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	39
Details	Repeat: {"description":"WD 2"}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	41
Details	Repeat: {"description":"WD 3"}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	39
Details	Repeat: {"description":"WD 4"}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	39
Details	Repeat: {"description":"WD 5"}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	34
Details	Repeat: {"description":"WD 6"}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	16
Details	Modified residue: {"description":"Methionine (R)-sulfoxide","evidences":[{"source":"UniProtKB","id":"P68134","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	8
Details	Modified residue: {"description":"Tele-methylhistidine","evidences":[{"source":"PubMed","id":"12356759","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1MDU","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	8
Details	Modified residue: {"description":"N6-methyllysine","evidences":[{"source":"UniProtKB","id":"P68133","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)