Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8DG7

Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB - complex III

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003006	biological_process	developmental process involved in reproduction
A	0003723	molecular_function	RNA binding
A	0003727	molecular_function	single-stranded RNA binding
A	0004386	molecular_function	helicase activity
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004521	molecular_function	RNA endonuclease activity
A	0004525	molecular_function	ribonuclease III activity
A	0004530	molecular_function	deoxyribonuclease I activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006309	biological_process	apoptotic DNA fragmentation
A	0006396	biological_process	RNA processing
A	0006950	biological_process	response to stress
A	0007279	biological_process	pole cell formation
A	0007294	biological_process	germarium-derived oocyte fate determination
A	0007367	biological_process	segment polarity determination
A	0009653	biological_process	anatomical structure morphogenesis
A	0016442	cellular_component	RISC complex
A	0016787	molecular_function	hydrolase activity
A	0016891	molecular_function	RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism
A	0030422	biological_process	siRNA processing
A	0030727	biological_process	germarium-derived female germ-line cyst formation
A	0031047	biological_process	regulatory ncRNA-mediated gene silencing
A	0031054	biological_process	pre-miRNA processing
A	0035194	biological_process	regulatory ncRNA-mediated post-transcriptional gene silencing
A	0035196	biological_process	miRNA processing
A	0042078	biological_process	germ-line stem cell division
A	0042594	biological_process	response to starvation
A	0045448	biological_process	mitotic cell cycle, embryonic
A	0046872	molecular_function	metal ion binding
A	0048731	biological_process	system development
A	0048813	biological_process	dendrite morphogenesis
A	0070578	cellular_component	RISC-loading complex
A	0070883	molecular_function	pre-miRNA binding
A	0070922	biological_process	RISC complex assembly
K	0003723	molecular_function	RNA binding
K	0003725	molecular_function	double-stranded RNA binding
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0007417	biological_process	central nervous system development
K	0009047	biological_process	dosage compensation by hyperactivation of X chromosome
K	0010468	biological_process	regulation of gene expression
K	0010586	biological_process	miRNA metabolic process
K	0016442	cellular_component	RISC complex
K	0030422	biological_process	siRNA processing
K	0030718	biological_process	germ-line stem cell population maintenance
K	0031047	biological_process	regulatory ncRNA-mediated gene silencing
K	0031054	biological_process	pre-miRNA processing
K	0035197	molecular_function	siRNA binding
K	0048132	biological_process	female germ-line stem cell asymmetric division
K	0048477	biological_process	oogenesis
K	0070578	cellular_component	RISC-loading complex
K	0070920	biological_process	regulation of regulatory ncRNA processing
K	0070922	biological_process	RISC complex assembly
K	0080090	biological_process	regulation of primary metabolic process
K	1905172	molecular_function	RISC complex binding

Functional Information from PROSITE/UniProt

site_id	PS00517
Number of Residues	9
Details	RNASE_3_1 Ribonuclease III family signature. QRLEFLGDA

Chain	Residue	Details
A	GLN2029-ALA2037

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	95
Details	Domain: {"description":"Dicer dsRNA-binding fold","evidences":[{"source":"PROSITE-ProRule","id":"PRU00657","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	146
Details	Domain: {"description":"PAZ","evidences":[{"source":"PROSITE-ProRule","id":"PRU00142","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	110
Details	Region: {"description":"Essential for miRNA substrate recognition","evidences":[{"source":"PubMed","id":"21926993","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	33
Details	Region: {"description":"Wing domain","evidences":[{"source":"PubMed","id":"36182693","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	145
Details	Region: {"description":"Platform domain","evidences":[{"source":"UniProtKB","id":"A1ZAW0","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	7
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36182693","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8DG7","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36182693","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8DG5","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8DG7","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q9UPY3","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	1
Details	Site: {"description":"Important for activity","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	68
Details	Domain: {"description":"DRBM 3","evidences":[{"source":"PROSITE-ProRule","id":"PRU00266","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	71
Details	Region: {"description":"Sufficent for binding to Dcr-1","evidences":[{"source":"PubMed","id":"26769856","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)