8DFV
Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB - complex IIa
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0003006 | biological_process | developmental process involved in reproduction |
| A | 0003723 | molecular_function | RNA binding |
| A | 0003727 | molecular_function | single-stranded RNA binding |
| A | 0004386 | molecular_function | helicase activity |
| A | 0004518 | molecular_function | nuclease activity |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0004521 | molecular_function | RNA endonuclease activity |
| A | 0004525 | molecular_function | ribonuclease III activity |
| A | 0004530 | molecular_function | deoxyribonuclease I activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006309 | biological_process | apoptotic DNA fragmentation |
| A | 0006396 | biological_process | RNA processing |
| A | 0006950 | biological_process | response to stress |
| A | 0007279 | biological_process | pole cell formation |
| A | 0007294 | biological_process | germarium-derived oocyte fate determination |
| A | 0007367 | biological_process | segment polarity determination |
| A | 0009653 | biological_process | anatomical structure morphogenesis |
| A | 0016442 | cellular_component | RISC complex |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016891 | molecular_function | RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism |
| A | 0030422 | biological_process | siRNA processing |
| A | 0030727 | biological_process | germarium-derived female germ-line cyst formation |
| A | 0031047 | biological_process | regulatory ncRNA-mediated gene silencing |
| A | 0031054 | biological_process | pre-miRNA processing |
| A | 0035194 | biological_process | regulatory ncRNA-mediated post-transcriptional gene silencing |
| A | 0035196 | biological_process | miRNA processing |
| A | 0042078 | biological_process | germ-line stem cell division |
| A | 0042594 | biological_process | response to starvation |
| A | 0045448 | biological_process | mitotic cell cycle, embryonic |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0048731 | biological_process | system development |
| A | 0048813 | biological_process | dendrite morphogenesis |
| A | 0070578 | cellular_component | RISC-loading complex |
| A | 0070883 | molecular_function | pre-miRNA binding |
| A | 0070922 | biological_process | RISC complex assembly |
| K | 0003723 | molecular_function | RNA binding |
| K | 0003725 | molecular_function | double-stranded RNA binding |
| K | 0005515 | molecular_function | protein binding |
| K | 0005634 | cellular_component | nucleus |
| K | 0005737 | cellular_component | cytoplasm |
| K | 0005829 | cellular_component | cytosol |
| K | 0007417 | biological_process | central nervous system development |
| K | 0009047 | biological_process | dosage compensation by hyperactivation of X chromosome |
| K | 0010468 | biological_process | regulation of gene expression |
| K | 0010586 | biological_process | miRNA metabolic process |
| K | 0016442 | cellular_component | RISC complex |
| K | 0030422 | biological_process | siRNA processing |
| K | 0030718 | biological_process | germ-line stem cell population maintenance |
| K | 0031047 | biological_process | regulatory ncRNA-mediated gene silencing |
| K | 0031054 | biological_process | pre-miRNA processing |
| K | 0035197 | molecular_function | siRNA binding |
| K | 0048132 | biological_process | female germ-line stem cell asymmetric division |
| K | 0048477 | biological_process | oogenesis |
| K | 0070578 | cellular_component | RISC-loading complex |
| K | 0070920 | biological_process | regulation of regulatory ncRNA processing |
| K | 0070922 | biological_process | RISC complex assembly |
| K | 0080090 | biological_process | regulation of primary metabolic process |
| K | 1905172 | molecular_function | RISC complex binding |
Functional Information from PROSITE/UniProt
| site_id | PS00517 |
| Number of Residues | 9 |
| Details | RNASE_3_1 Ribonuclease III family signature. QRLEFLGDA |
| Chain | Residue | Details |
| A | GLN2029-ALA2037 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 95 |
| Details | Domain: {"description":"Dicer dsRNA-binding fold","evidences":[{"source":"PROSITE-ProRule","id":"PRU00657","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 146 |
| Details | Domain: {"description":"PAZ","evidences":[{"source":"PROSITE-ProRule","id":"PRU00142","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 110 |
| Details | Region: {"description":"Essential for miRNA substrate recognition","evidences":[{"source":"PubMed","id":"21926993","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 33 |
| Details | Region: {"description":"Wing domain","evidences":[{"source":"PubMed","id":"36182693","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 145 |
| Details | Region: {"description":"Platform domain","evidences":[{"source":"UniProtKB","id":"A1ZAW0","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 7 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 3 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"36182693","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8DG7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"36182693","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8DG5","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8DG7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 3 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"Q9UPY3","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | Site: {"description":"Important for activity","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 68 |
| Details | Domain: {"description":"DRBM 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00266","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 68 |
| Details | Domain: {"description":"DRBM 3","evidences":[{"source":"PROSITE-ProRule","id":"PRU00266","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 1 |
| Details | Region: {"description":"Necessary for binding pre-miRNA","evidences":[{"source":"PubMed","id":"17666393","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI14 |
| Number of Residues | 71 |
| Details | Region: {"description":"Sufficent for binding to Dcr-1","evidences":[{"source":"PubMed","id":"26769856","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
