8D7K

Bifunctional Inhibition of Neutrophil Elastase and Cathepsin G by Eap2 from S. aureus

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0002020	molecular_function	protease binding
A	0002438	biological_process	acute inflammatory response to antigenic stimulus
A	0002523	biological_process	leukocyte migration involved in inflammatory response
A	0002812	biological_process	biosynthetic process of antibacterial peptides active against Gram-negative bacteria
A	0003714	molecular_function	transcription corepressor activity
A	0004175	molecular_function	endopeptidase activity
A	0004252	molecular_function	serine-type endopeptidase activity
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005615	cellular_component	extracellular space
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006508	biological_process	proteolysis
A	0006874	biological_process	intracellular calcium ion homeostasis
A	0006909	biological_process	phagocytosis
A	0008201	molecular_function	heparin binding
A	0008233	molecular_function	peptidase activity
A	0008236	molecular_function	serine-type peptidase activity
A	0009411	biological_process	response to UV
A	0009986	cellular_component	cell surface
A	0016787	molecular_function	hydrolase activity
A	0017053	cellular_component	transcription repressor complex
A	0019955	molecular_function	cytokine binding
A	0022617	biological_process	extracellular matrix disassembly
A	0030141	cellular_component	secretory granule
A	0030163	biological_process	protein catabolic process
A	0031012	cellular_component	extracellular matrix
A	0031410	cellular_component	cytoplasmic vesicle
A	0031452	biological_process	negative regulation of heterochromatin formation
A	0032682	biological_process	negative regulation of chemokine production
A	0032717	biological_process	negative regulation of interleukin-8 production
A	0032757	biological_process	positive regulation of interleukin-8 production
A	0035578	cellular_component	azurophil granule lumen
A	0035580	cellular_component	specific granule lumen
A	0042582	cellular_component	azurophil granule
A	0042742	biological_process	defense response to bacterium
A	0043406	biological_process	positive regulation of MAP kinase activity
A	0045335	cellular_component	phagocytic vesicle
A	0048661	biological_process	positive regulation of smooth muscle cell proliferation
A	0050728	biological_process	negative regulation of inflammatory response
A	0050922	biological_process	negative regulation of chemotaxis
A	0070062	cellular_component	extracellular exosome
A	0070269	biological_process	pyroptotic inflammatory response
A	0070944	biological_process	neutrophil-mediated killing of bacterium
A	0070945	biological_process	neutrophil-mediated killing of gram-negative bacterium
A	0070947	biological_process	neutrophil-mediated killing of fungus
A	0140644	cellular_component	neutrophil extracellular trap
A	0140645	biological_process	neutrophil extracellular trap formation
A	1903238	biological_process	positive regulation of leukocyte tethering or rolling
B	0005576	cellular_component	extracellular region
C	0002003	biological_process	angiotensin maturation
C	0002548	biological_process	monocyte chemotaxis
C	0004252	molecular_function	serine-type endopeptidase activity
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005615	cellular_component	extracellular space
C	0005634	cellular_component	nucleus
C	0005764	cellular_component	lysosome
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006508	biological_process	proteolysis
C	0006935	biological_process	chemotaxis
C	0006955	biological_process	immune response
C	0008201	molecular_function	heparin binding
C	0008233	molecular_function	peptidase activity
C	0008236	molecular_function	serine-type peptidase activity
C	0010494	cellular_component	cytoplasmic stress granule
C	0016020	cellular_component	membrane
C	0016485	biological_process	protein processing
C	0016787	molecular_function	hydrolase activity
C	0019221	biological_process	cytokine-mediated signaling pathway
C	0019731	biological_process	antibacterial humoral response
C	0022617	biological_process	extracellular matrix disassembly
C	0030141	cellular_component	secretory granule
C	0030168	biological_process	platelet activation
C	0031012	cellular_component	extracellular matrix
C	0035578	cellular_component	azurophil granule lumen
C	0035590	biological_process	purinergic nucleotide receptor signaling pathway
C	0042119	biological_process	neutrophil activation
C	0042742	biological_process	defense response to bacterium
C	0048018	molecular_function	receptor ligand activity
C	0050829	biological_process	defense response to Gram-negative bacterium
C	0050830	biological_process	defense response to Gram-positive bacterium
C	0050868	biological_process	negative regulation of T cell activation
C	0051604	biological_process	protein maturation
C	0070062	cellular_component	extracellular exosome
C	0071222	biological_process	cellular response to lipopolysaccharide
C	0089720	molecular_function	caspase binding
C	0098786	biological_process	biofilm matrix disassembly
C	1901731	biological_process	positive regulation of platelet aggregation
D	0000122	biological_process	negative regulation of transcription by RNA polymerase II
D	0002020	molecular_function	protease binding
D	0002438	biological_process	acute inflammatory response to antigenic stimulus
D	0002523	biological_process	leukocyte migration involved in inflammatory response
D	0002812	biological_process	biosynthetic process of antibacterial peptides active against Gram-negative bacteria
D	0003714	molecular_function	transcription corepressor activity
D	0004175	molecular_function	endopeptidase activity
D	0004252	molecular_function	serine-type endopeptidase activity
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005615	cellular_component	extracellular space
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006508	biological_process	proteolysis
D	0006874	biological_process	intracellular calcium ion homeostasis
D	0006909	biological_process	phagocytosis
D	0008201	molecular_function	heparin binding
D	0008233	molecular_function	peptidase activity
D	0008236	molecular_function	serine-type peptidase activity
D	0009411	biological_process	response to UV
D	0009986	cellular_component	cell surface
D	0016787	molecular_function	hydrolase activity
D	0017053	cellular_component	transcription repressor complex
D	0019955	molecular_function	cytokine binding
D	0022617	biological_process	extracellular matrix disassembly
D	0030141	cellular_component	secretory granule
D	0030163	biological_process	protein catabolic process
D	0031012	cellular_component	extracellular matrix
D	0031410	cellular_component	cytoplasmic vesicle
D	0031452	biological_process	negative regulation of heterochromatin formation
D	0032682	biological_process	negative regulation of chemokine production
D	0032717	biological_process	negative regulation of interleukin-8 production
D	0032757	biological_process	positive regulation of interleukin-8 production
D	0035578	cellular_component	azurophil granule lumen
D	0035580	cellular_component	specific granule lumen
D	0042582	cellular_component	azurophil granule
D	0042742	biological_process	defense response to bacterium
D	0043406	biological_process	positive regulation of MAP kinase activity
D	0045335	cellular_component	phagocytic vesicle
D	0048661	biological_process	positive regulation of smooth muscle cell proliferation
D	0050728	biological_process	negative regulation of inflammatory response
D	0050922	biological_process	negative regulation of chemotaxis
D	0070062	cellular_component	extracellular exosome
D	0070269	biological_process	pyroptotic inflammatory response
D	0070944	biological_process	neutrophil-mediated killing of bacterium
D	0070945	biological_process	neutrophil-mediated killing of gram-negative bacterium
D	0070947	biological_process	neutrophil-mediated killing of fungus
D	0140644	cellular_component	neutrophil extracellular trap
D	0140645	biological_process	neutrophil extracellular trap formation
D	1903238	biological_process	positive regulation of leukocyte tethering or rolling
E	0005576	cellular_component	extracellular region
F	0002003	biological_process	angiotensin maturation
F	0002548	biological_process	monocyte chemotaxis
F	0004252	molecular_function	serine-type endopeptidase activity
F	0005515	molecular_function	protein binding
F	0005576	cellular_component	extracellular region
F	0005615	cellular_component	extracellular space
F	0005634	cellular_component	nucleus
F	0005764	cellular_component	lysosome
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0006508	biological_process	proteolysis
F	0006935	biological_process	chemotaxis
F	0006955	biological_process	immune response
F	0008201	molecular_function	heparin binding
F	0008233	molecular_function	peptidase activity
F	0008236	molecular_function	serine-type peptidase activity
F	0010494	cellular_component	cytoplasmic stress granule
F	0016020	cellular_component	membrane
F	0016485	biological_process	protein processing
F	0016787	molecular_function	hydrolase activity
F	0019221	biological_process	cytokine-mediated signaling pathway
F	0019731	biological_process	antibacterial humoral response
F	0022617	biological_process	extracellular matrix disassembly
F	0030141	cellular_component	secretory granule
F	0030168	biological_process	platelet activation
F	0031012	cellular_component	extracellular matrix
F	0035578	cellular_component	azurophil granule lumen
F	0035590	biological_process	purinergic nucleotide receptor signaling pathway
F	0042119	biological_process	neutrophil activation
F	0042742	biological_process	defense response to bacterium
F	0048018	molecular_function	receptor ligand activity
F	0050829	biological_process	defense response to Gram-negative bacterium
F	0050830	biological_process	defense response to Gram-positive bacterium
F	0050868	biological_process	negative regulation of T cell activation
F	0051604	biological_process	protein maturation
F	0070062	cellular_component	extracellular exosome
F	0071222	biological_process	cellular response to lipopolysaccharide
F	0089720	molecular_function	caspase binding
F	0098786	biological_process	biofilm matrix disassembly
F	1901731	biological_process	positive regulation of platelet aggregation
G	0000122	biological_process	negative regulation of transcription by RNA polymerase II
G	0002020	molecular_function	protease binding
G	0002438	biological_process	acute inflammatory response to antigenic stimulus
G	0002523	biological_process	leukocyte migration involved in inflammatory response
G	0002812	biological_process	biosynthetic process of antibacterial peptides active against Gram-negative bacteria
G	0003714	molecular_function	transcription corepressor activity
G	0004175	molecular_function	endopeptidase activity
G	0004252	molecular_function	serine-type endopeptidase activity
G	0005515	molecular_function	protein binding
G	0005576	cellular_component	extracellular region
G	0005615	cellular_component	extracellular space
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006508	biological_process	proteolysis
G	0006874	biological_process	intracellular calcium ion homeostasis
G	0006909	biological_process	phagocytosis
G	0008201	molecular_function	heparin binding
G	0008233	molecular_function	peptidase activity
G	0008236	molecular_function	serine-type peptidase activity
G	0009411	biological_process	response to UV
G	0009986	cellular_component	cell surface
G	0016787	molecular_function	hydrolase activity
G	0017053	cellular_component	transcription repressor complex
G	0019955	molecular_function	cytokine binding
G	0022617	biological_process	extracellular matrix disassembly
G	0030141	cellular_component	secretory granule
G	0030163	biological_process	protein catabolic process
G	0031012	cellular_component	extracellular matrix
G	0031410	cellular_component	cytoplasmic vesicle
G	0031452	biological_process	negative regulation of heterochromatin formation
G	0032682	biological_process	negative regulation of chemokine production
G	0032717	biological_process	negative regulation of interleukin-8 production
G	0032757	biological_process	positive regulation of interleukin-8 production
G	0035578	cellular_component	azurophil granule lumen
G	0035580	cellular_component	specific granule lumen
G	0042582	cellular_component	azurophil granule
G	0042742	biological_process	defense response to bacterium
G	0043406	biological_process	positive regulation of MAP kinase activity
G	0045335	cellular_component	phagocytic vesicle
G	0048661	biological_process	positive regulation of smooth muscle cell proliferation
G	0050728	biological_process	negative regulation of inflammatory response
G	0050922	biological_process	negative regulation of chemotaxis
G	0070062	cellular_component	extracellular exosome
G	0070269	biological_process	pyroptotic inflammatory response
G	0070944	biological_process	neutrophil-mediated killing of bacterium
G	0070945	biological_process	neutrophil-mediated killing of gram-negative bacterium
G	0070947	biological_process	neutrophil-mediated killing of fungus
G	0140644	cellular_component	neutrophil extracellular trap
G	0140645	biological_process	neutrophil extracellular trap formation
G	1903238	biological_process	positive regulation of leukocyte tethering or rolling
H	0005576	cellular_component	extracellular region
I	0002003	biological_process	angiotensin maturation
I	0002548	biological_process	monocyte chemotaxis
I	0004252	molecular_function	serine-type endopeptidase activity
I	0005515	molecular_function	protein binding
I	0005576	cellular_component	extracellular region
I	0005615	cellular_component	extracellular space
I	0005634	cellular_component	nucleus
I	0005764	cellular_component	lysosome
I	0005829	cellular_component	cytosol
I	0005886	cellular_component	plasma membrane
I	0006508	biological_process	proteolysis
I	0006935	biological_process	chemotaxis
I	0006955	biological_process	immune response
I	0008201	molecular_function	heparin binding
I	0008233	molecular_function	peptidase activity
I	0008236	molecular_function	serine-type peptidase activity
I	0010494	cellular_component	cytoplasmic stress granule
I	0016020	cellular_component	membrane
I	0016485	biological_process	protein processing
I	0016787	molecular_function	hydrolase activity
I	0019221	biological_process	cytokine-mediated signaling pathway
I	0019731	biological_process	antibacterial humoral response
I	0022617	biological_process	extracellular matrix disassembly
I	0030141	cellular_component	secretory granule
I	0030168	biological_process	platelet activation
I	0031012	cellular_component	extracellular matrix
I	0035578	cellular_component	azurophil granule lumen
I	0035590	biological_process	purinergic nucleotide receptor signaling pathway
I	0042119	biological_process	neutrophil activation
I	0042742	biological_process	defense response to bacterium
I	0048018	molecular_function	receptor ligand activity
I	0050829	biological_process	defense response to Gram-negative bacterium
I	0050830	biological_process	defense response to Gram-positive bacterium
I	0050868	biological_process	negative regulation of T cell activation
I	0051604	biological_process	protein maturation
I	0070062	cellular_component	extracellular exosome
I	0071222	biological_process	cellular response to lipopolysaccharide
I	0089720	molecular_function	caspase binding
I	0098786	biological_process	biofilm matrix disassembly
I	1901731	biological_process	positive regulation of platelet aggregation
J	0000122	biological_process	negative regulation of transcription by RNA polymerase II
J	0002020	molecular_function	protease binding
J	0002438	biological_process	acute inflammatory response to antigenic stimulus
J	0002523	biological_process	leukocyte migration involved in inflammatory response
J	0002812	biological_process	biosynthetic process of antibacterial peptides active against Gram-negative bacteria
J	0003714	molecular_function	transcription corepressor activity
J	0004175	molecular_function	endopeptidase activity
J	0004252	molecular_function	serine-type endopeptidase activity
J	0005515	molecular_function	protein binding
J	0005576	cellular_component	extracellular region
J	0005615	cellular_component	extracellular space
J	0005634	cellular_component	nucleus
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0006508	biological_process	proteolysis
J	0006874	biological_process	intracellular calcium ion homeostasis
J	0006909	biological_process	phagocytosis
J	0008201	molecular_function	heparin binding
J	0008233	molecular_function	peptidase activity
J	0008236	molecular_function	serine-type peptidase activity
J	0009411	biological_process	response to UV
J	0009986	cellular_component	cell surface
J	0016787	molecular_function	hydrolase activity
J	0017053	cellular_component	transcription repressor complex
J	0019955	molecular_function	cytokine binding
J	0022617	biological_process	extracellular matrix disassembly
J	0030141	cellular_component	secretory granule
J	0030163	biological_process	protein catabolic process
J	0031012	cellular_component	extracellular matrix
J	0031410	cellular_component	cytoplasmic vesicle
J	0031452	biological_process	negative regulation of heterochromatin formation
J	0032682	biological_process	negative regulation of chemokine production
J	0032717	biological_process	negative regulation of interleukin-8 production
J	0032757	biological_process	positive regulation of interleukin-8 production
J	0035578	cellular_component	azurophil granule lumen
J	0035580	cellular_component	specific granule lumen
J	0042582	cellular_component	azurophil granule
J	0042742	biological_process	defense response to bacterium
J	0043406	biological_process	positive regulation of MAP kinase activity
J	0045335	cellular_component	phagocytic vesicle
J	0048661	biological_process	positive regulation of smooth muscle cell proliferation
J	0050728	biological_process	negative regulation of inflammatory response
J	0050922	biological_process	negative regulation of chemotaxis
J	0070062	cellular_component	extracellular exosome
J	0070269	biological_process	pyroptotic inflammatory response
J	0070944	biological_process	neutrophil-mediated killing of bacterium
J	0070945	biological_process	neutrophil-mediated killing of gram-negative bacterium
J	0070947	biological_process	neutrophil-mediated killing of fungus
J	0140644	cellular_component	neutrophil extracellular trap
J	0140645	biological_process	neutrophil extracellular trap formation
J	1903238	biological_process	positive regulation of leukocyte tethering or rolling
K	0005576	cellular_component	extracellular region
L	0002003	biological_process	angiotensin maturation
L	0002548	biological_process	monocyte chemotaxis
L	0004252	molecular_function	serine-type endopeptidase activity
L	0005515	molecular_function	protein binding
L	0005576	cellular_component	extracellular region
L	0005615	cellular_component	extracellular space
L	0005634	cellular_component	nucleus
L	0005764	cellular_component	lysosome
L	0005829	cellular_component	cytosol
L	0005886	cellular_component	plasma membrane
L	0006508	biological_process	proteolysis
L	0006935	biological_process	chemotaxis
L	0006955	biological_process	immune response
L	0008201	molecular_function	heparin binding
L	0008233	molecular_function	peptidase activity
L	0008236	molecular_function	serine-type peptidase activity
L	0010494	cellular_component	cytoplasmic stress granule
L	0016020	cellular_component	membrane
L	0016485	biological_process	protein processing
L	0016787	molecular_function	hydrolase activity
L	0019221	biological_process	cytokine-mediated signaling pathway
L	0019731	biological_process	antibacterial humoral response
L	0022617	biological_process	extracellular matrix disassembly
L	0030141	cellular_component	secretory granule
L	0030168	biological_process	platelet activation
L	0031012	cellular_component	extracellular matrix
L	0035578	cellular_component	azurophil granule lumen
L	0035590	biological_process	purinergic nucleotide receptor signaling pathway
L	0042119	biological_process	neutrophil activation
L	0042742	biological_process	defense response to bacterium
L	0048018	molecular_function	receptor ligand activity
L	0050829	biological_process	defense response to Gram-negative bacterium
L	0050830	biological_process	defense response to Gram-positive bacterium
L	0050868	biological_process	negative regulation of T cell activation
L	0051604	biological_process	protein maturation
L	0070062	cellular_component	extracellular exosome
L	0071222	biological_process	cellular response to lipopolysaccharide
L	0089720	molecular_function	caspase binding
L	0098786	biological_process	biofilm matrix disassembly
L	1901731	biological_process	positive regulation of platelet aggregation

Functional Information from PROSITE/UniProt

site_id	PS00134
Number of Residues	6
Details	TRYPSIN_HIS Serine proteases, trypsin family, histidine active site. MSAAHC

Chain	Residue	Details
A	MET65-CYS70
C	LEU55-CYS60

---

site_id	PS00135
Number of Residues	12
Details	TRYPSIN_SER Serine proteases, trypsin family, serine active site. GVcfGDSGSPLV

Chain	Residue	Details
A	GLY195-VAL206
C	ALA190-LEU201

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1756
Details	Domain: {"description":"Peptidase S1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00274","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	72
Details	Region: {"description":"Important for antimicrobial activity","evidences":[{"source":"PubMed","id":"2116408","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	12
Details	Active site: {"description":"Charge relay system","evidences":[{"source":"PROSITE-ProRule","id":"PRU00274","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Glycosylation: {"description":"N-linked (GlcNAc...) (paucimannose) asparagine; alternate","evidences":[{"source":"PubMed","id":"26274980","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	12
Details	Active site: {"description":"Charge relay system","evidences":[{"source":"PubMed","id":"2911584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"26274980","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"3550808","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"3550808","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---