7ZP5
Crystal structure of designed photoenzyme EnT1.0
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005509 | molecular_function | calcium ion binding |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0047862 | molecular_function | diisopropyl-fluorophosphatase activity |
| B | 0005509 | molecular_function | calcium ion binding |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0047862 | molecular_function | diisopropyl-fluorophosphatase activity |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor => ECO:0000269|PubMed:15966726 |
| Chain | Residue | Details |
| A | HIS287 | |
| B | HIS287 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000305 |
| Chain | Residue | Details |
| A | ALA21 | |
| A | ALA120 | |
| A | ALA175 | |
| A | ALA229 | |
| B | ALA21 | |
| B | ALA120 | |
| B | ALA175 | |
| B | ALA229 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11435114, ECO:0000269|PubMed:14501113 |
| Chain | Residue | Details |
| A | ASP232 | |
| A | LEU273 | |
| A | HIS274 | |
| B | ASP232 | |
| B | LEU273 | |
| B | HIS274 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 686 |
| Chain | Residue | Details |
| A | ALA21 | increase nucleophilicity, metal ligand, proton acceptor |
| A | TYR37 | electrostatic stabiliser, increase basicity |
| A | ALA120 | metal ligand |
| A | ALA175 | metal ligand |
| A | ALA229 | covalently attached, electrofuge, electrophile, increase nucleophilicity, metal ligand, nucleophile, proton acceptor |
| A | HIS287 | increase nucleophilicity, proton acceptor |
| site_id | MCSA2 |
| Number of Residues | 6 |
| Details | M-CSA 686 |
| Chain | Residue | Details |
| B | ALA21 | increase nucleophilicity, metal ligand, proton acceptor |
| B | TYR37 | electrostatic stabiliser, increase basicity |
| B | ALA120 | metal ligand |
| B | ALA175 | metal ligand |
| B | ALA229 | covalently attached, electrofuge, electrophile, increase nucleophilicity, metal ligand, nucleophile, proton acceptor |
| B | HIS287 | increase nucleophilicity, proton acceptor |
