Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7ZBN

Cryo-EM structure of the human GS-GN complex in the inhibited state

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004373	molecular_function	glycogen (starch) synthase activity
A	0005515	molecular_function	protein binding
A	0005536	molecular_function	D-glucose binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005977	biological_process	glycogen metabolic process
A	0005978	biological_process	glycogen biosynthetic process
A	0007507	biological_process	heart development
A	0016020	cellular_component	membrane
A	0016234	cellular_component	inclusion body
A	0016757	molecular_function	glycosyltransferase activity
A	0061547	molecular_function	glycogen synthase activity, transferring glucose-1-phosphate
B	0004373	molecular_function	glycogen (starch) synthase activity
B	0005515	molecular_function	protein binding
B	0005536	molecular_function	D-glucose binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005977	biological_process	glycogen metabolic process
B	0005978	biological_process	glycogen biosynthetic process
B	0007507	biological_process	heart development
B	0016020	cellular_component	membrane
B	0016234	cellular_component	inclusion body
B	0016757	molecular_function	glycosyltransferase activity
B	0061547	molecular_function	glycogen synthase activity, transferring glucose-1-phosphate
C	0004373	molecular_function	glycogen (starch) synthase activity
C	0005515	molecular_function	protein binding
C	0005536	molecular_function	D-glucose binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005977	biological_process	glycogen metabolic process
C	0005978	biological_process	glycogen biosynthetic process
C	0007507	biological_process	heart development
C	0016020	cellular_component	membrane
C	0016234	cellular_component	inclusion body
C	0016757	molecular_function	glycosyltransferase activity
C	0061547	molecular_function	glycogen synthase activity, transferring glucose-1-phosphate
D	0004373	molecular_function	glycogen (starch) synthase activity
D	0005515	molecular_function	protein binding
D	0005536	molecular_function	D-glucose binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005977	biological_process	glycogen metabolic process
D	0005978	biological_process	glycogen biosynthetic process
D	0007507	biological_process	heart development
D	0016020	cellular_component	membrane
D	0016234	cellular_component	inclusion body
D	0016757	molecular_function	glycosyltransferase activity
D	0061547	molecular_function	glycogen synthase activity, transferring glucose-1-phosphate
E	0005515	molecular_function	protein binding
E	0005576	cellular_component	extracellular region
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005978	biological_process	glycogen biosynthetic process
E	0008466	molecular_function	glycogenin glucosyltransferase activity
E	0016020	cellular_component	membrane
E	0016740	molecular_function	transferase activity
E	0016757	molecular_function	glycosyltransferase activity
E	0030145	molecular_function	manganese ion binding
E	0034774	cellular_component	secretory granule lumen
E	0042803	molecular_function	protein homodimerization activity
E	0043202	cellular_component	lysosomal lumen
E	0046872	molecular_function	metal ion binding
E	0102751	molecular_function	UDP-alpha-D-glucose:glucosyl-glycogenin alpha-D-glucosyltransferase activity
E	1904813	cellular_component	ficolin-1-rich granule lumen
F	0005515	molecular_function	protein binding
F	0005576	cellular_component	extracellular region
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005978	biological_process	glycogen biosynthetic process
F	0008466	molecular_function	glycogenin glucosyltransferase activity
F	0016020	cellular_component	membrane
F	0016740	molecular_function	transferase activity
F	0016757	molecular_function	glycosyltransferase activity
F	0030145	molecular_function	manganese ion binding
F	0034774	cellular_component	secretory granule lumen
F	0042803	molecular_function	protein homodimerization activity
F	0043202	cellular_component	lysosomal lumen
F	0046872	molecular_function	metal ion binding
F	0102751	molecular_function	UDP-alpha-D-glucose:glucosyl-glycogenin alpha-D-glucosyltransferase activity
F	1904813	cellular_component	ficolin-1-rich granule lumen
G	0005515	molecular_function	protein binding
G	0005576	cellular_component	extracellular region
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005978	biological_process	glycogen biosynthetic process
G	0008466	molecular_function	glycogenin glucosyltransferase activity
G	0016020	cellular_component	membrane
G	0016740	molecular_function	transferase activity
G	0016757	molecular_function	glycosyltransferase activity
G	0030145	molecular_function	manganese ion binding
G	0034774	cellular_component	secretory granule lumen
G	0042803	molecular_function	protein homodimerization activity
G	0043202	cellular_component	lysosomal lumen
G	0046872	molecular_function	metal ion binding
G	0102751	molecular_function	UDP-alpha-D-glucose:glucosyl-glycogenin alpha-D-glucosyltransferase activity
G	1904813	cellular_component	ficolin-1-rich granule lumen
H	0005515	molecular_function	protein binding
H	0005576	cellular_component	extracellular region
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005978	biological_process	glycogen biosynthetic process
H	0008466	molecular_function	glycogenin glucosyltransferase activity
H	0016020	cellular_component	membrane
H	0016740	molecular_function	transferase activity
H	0016757	molecular_function	glycosyltransferase activity
H	0030145	molecular_function	manganese ion binding
H	0034774	cellular_component	secretory granule lumen
H	0042803	molecular_function	protein homodimerization activity
H	0043202	cellular_component	lysosomal lumen
H	0046872	molecular_function	metal ion binding
H	0102751	molecular_function	UDP-alpha-D-glucose:glucosyl-glycogenin alpha-D-glucosyltransferase activity
H	1904813	cellular_component	ficolin-1-rich granule lumen

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:22160680, ECO:0000269\|PubMed:30356213, ECO:0007744\|PDB:3RMW, ECO:0007744\|PDB:3T7O

Chain	Residue	Details
E	LEU9
F	LEU9
G	LEU9
H	LEU9
D	LYS39
D	HIS297
D	LYS301
D	THR515

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:22160680, ECO:0007744\|PDB:3T7M, ECO:0007744\|PDB:3T7N, ECO:0007744\|PDB:3T7O, ECO:0007744\|PDB:3U2U, ECO:0007744\|PDB:3U2V, ECO:0007744\|PDB:3U2W, ECO:0007744\|PDB:3U2X

Chain	Residue	Details
E	ARG77
D	GLU510
D	TRP512
D	GLY513
F	ARG77
G	ARG77
H	ARG77
B	TRP512
B	GLY513
D	HIS205
D	ARG211
D	ARG331

site_id	SWS_FT_FI3
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:22160680, ECO:0000269\|PubMed:30356213, ECO:0007744\|PDB:3QVB, ECO:0007744\|PDB:3RMV, ECO:0007744\|PDB:3RMW, ECO:0007744\|PDB:3T7M, ECO:0007744\|PDB:3T7N, ECO:0007744\|PDB:3T7O, ECO:0007744\|PDB:3U2T, ECO:0007744\|PDB:3U2U, ECO:0007744\|PDB:3U2V, ECO:0007744\|PDB:3U2W, ECO:0007744\|PDB:3U2X

Chain	Residue	Details
E	ASP102
H	ASP102
H	ASP104
H	HIS212
E	ASP104
E	HIS212
F	ASP102
F	ASP104
F	HIS212
G	ASP102
G	ASP104
G	HIS212

site_id	SWS_FT_FI4
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269\|PubMed:22160680, ECO:0007744\|PDB:3RMW, ECO:0007744\|PDB:3T7O

Chain	Residue	Details
E	ASN133
E	ASP160
F	ASN133
F	ASP160
G	ASN133
G	ASP160
H	ASN133
H	ASP160

site_id	SWS_FT_FI5
Number of Residues	4
Details	SITE: Important for catalytic activity => ECO:0000250\|UniProtKB:P13280

Chain	Residue	Details
E	LYS86
F	LYS86
G	LYS86
H	LYS86

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: N-acetylthreonine => ECO:0007744\|PubMed:19413330, ECO:0007744\|PubMed:22223895

Chain	Residue	Details
E	ALA2
F	ALA2
G	ALA2
H	ALA2

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:P13280

Chain	Residue	Details
E	SER44
F	SER44
G	SER44
H	SER44

site_id	SWS_FT_FI8
Number of Residues	4
Details	CARBOHYD: O-linked (Glc...) tyrosine => ECO:0000269\|PubMed:22160680, ECO:0000269\|PubMed:30356213, ECO:0007744\|PDB:3U2U, ECO:0007744\|PDB:3U2V

Chain	Residue	Details
E	PHE195
F	PHE195
G	PHE195
H	PHE195

site_id	SWS_FT_FI9
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:18669648

Chain	Residue	Details
B	SER649
D	SER649

site_id	SWS_FT_FI10
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000269\|PubMed:35690592

Chain	Residue	Details
B	SER652
B	SER731
D	SER652
D	SER731

site_id	SWS_FT_FI11
Number of Residues	2
Details	MOD_RES: Phosphoserine; by GSK3-alpha and GSK3-beta => ECO:0000269\|PubMed:35690592

Chain	Residue	Details
B	SER653
D	SER653

site_id	SWS_FT_FI12
Number of Residues	2
Details	MOD_RES: Phosphoserine; by CK2 => ECO:0000269\|PubMed:35690592

Chain	Residue	Details
B	SER657
D	SER657

site_id	SWS_FT_FI13
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:24275569

Chain	Residue	Details
B	THR700
D	THR700

site_id	SWS_FT_FI14
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
B	SER710
D	SER710

site_id	SWS_FT_FI15
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0000250\|UniProtKB:Q9Z1E4

Chain	Residue	Details
B	THR721
D	THR721

site_id	SWS_FT_FI16
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000269\|PubMed:35690592, ECO:0007744\|PubMed:19690332, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
B	SER727
D	SER727

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)