Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7Y5X

CryoEM structure of PS2-containing gamma-secretase treated with MRK-560

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016020	cellular_component	membrane
A	0016485	biological_process	protein processing
B	0000139	cellular_component	Golgi membrane
B	0000776	cellular_component	kinetochore
B	0001666	biological_process	response to hypoxia
B	0005515	molecular_function	protein binding
B	0005637	cellular_component	nuclear inner membrane
B	0005764	cellular_component	lysosome
B	0005765	cellular_component	lysosomal membrane
B	0005769	cellular_component	early endosome
B	0005770	cellular_component	late endosome
B	0005783	cellular_component	endoplasmic reticulum
B	0005789	cellular_component	endoplasmic reticulum membrane
B	0005794	cellular_component	Golgi apparatus
B	0005813	cellular_component	centrosome
B	0005886	cellular_component	plasma membrane
B	0006509	biological_process	membrane protein ectodomain proteolysis
B	0007219	biological_process	Notch signaling pathway
B	0007220	biological_process	Notch receptor processing
B	0008021	cellular_component	synaptic vesicle
B	0016020	cellular_component	membrane
B	0016485	biological_process	protein processing
B	0031902	cellular_component	late endosome membrane
B	0031965	cellular_component	nuclear membrane
B	0032991	cellular_component	protein-containing complex
B	0034205	biological_process	amyloid-beta formation
B	0035333	biological_process	Notch receptor processing, ligand-dependent
B	0035556	biological_process	intracellular signal transduction
B	0042500	molecular_function	aspartic endopeptidase activity, intramembrane cleaving
B	0042734	cellular_component	presynaptic membrane
B	0042987	biological_process	amyloid precursor protein catabolic process
B	0044233	cellular_component	mitochondria-associated endoplasmic reticulum membrane contact site
B	0055074	biological_process	calcium ion homeostasis
B	0070765	cellular_component	gamma-secretase complex
B	0097060	cellular_component	synaptic membrane
B	0110097	biological_process	regulation of calcium import into the mitochondrion
C	0000139	cellular_component	Golgi membrane
C	0005515	molecular_function	protein binding
C	0005783	cellular_component	endoplasmic reticulum
C	0005789	cellular_component	endoplasmic reticulum membrane
C	0005794	cellular_component	Golgi apparatus
C	0005886	cellular_component	plasma membrane
C	0006508	biological_process	proteolysis
C	0006509	biological_process	membrane protein ectodomain proteolysis
C	0007219	biological_process	Notch signaling pathway
C	0007220	biological_process	Notch receptor processing
C	0010008	cellular_component	endosome membrane
C	0016020	cellular_component	membrane
C	0016485	biological_process	protein processing
C	0019899	molecular_function	enzyme binding
C	0030674	molecular_function	protein-macromolecule adaptor activity
C	0031293	biological_process	membrane protein intracellular domain proteolysis
C	0032580	cellular_component	Golgi cisterna membrane
C	0034205	biological_process	amyloid-beta formation
C	0042982	biological_process	amyloid precursor protein metabolic process
C	0042987	biological_process	amyloid precursor protein catabolic process
C	0043085	biological_process	positive regulation of catalytic activity
C	0061133	molecular_function	endopeptidase activator activity
C	0070765	cellular_component	gamma-secretase complex
D	0000139	cellular_component	Golgi membrane
D	0005515	molecular_function	protein binding
D	0005783	cellular_component	endoplasmic reticulum
D	0005789	cellular_component	endoplasmic reticulum membrane
D	0005794	cellular_component	Golgi apparatus
D	0005886	cellular_component	plasma membrane
D	0006509	biological_process	membrane protein ectodomain proteolysis
D	0007220	biological_process	Notch receptor processing
D	0010008	cellular_component	endosome membrane
D	0010950	biological_process	positive regulation of endopeptidase activity
D	0016020	cellular_component	membrane
D	0016485	biological_process	protein processing
D	0019899	molecular_function	enzyme binding
D	0031293	biological_process	membrane protein intracellular domain proteolysis
D	0032580	cellular_component	Golgi cisterna membrane
D	0034205	biological_process	amyloid-beta formation
D	0042149	biological_process	cellular response to glucose starvation
D	0042734	cellular_component	presynaptic membrane
D	0042982	biological_process	amyloid precursor protein metabolic process
D	0042987	biological_process	amyloid precursor protein catabolic process
D	0061133	molecular_function	endopeptidase activator activity
D	0070765	cellular_component	gamma-secretase complex
D	0140678	molecular_function	molecular function inhibitor activity
D	1904262	biological_process	negative regulation of TORC1 signaling

Functional Information from PROSITE/UniProt

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. VAATrlDsRSFFWNV

Chain	Residue	Details
A	VAL277-VAL291

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	635
Details	Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"26776682","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	20
Details	Transmembrane: {"description":"Helical","evidences":[{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"26776682","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	2
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30598546","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30630874","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5A63","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6IDF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6IYC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	8
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30598546","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30630874","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5A63","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6IDF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6IYC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	3
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	1
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"12754519","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30598546","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30630874","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5A63","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6IDF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6IYC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	1
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"30630874","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6IYC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	1
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"19349973","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	100
Details	Transmembrane: {"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	21
Details	Topological domain: {"description":"Lumenal","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	17
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	20
Details	Intramembrane: {"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	2
Details	Motif: {"description":"PAL"}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	1
Details	Active site: {"evidences":[{"source":"PubMed","id":"10652302","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	1
Details	Active site: {"evidences":[{"source":"PubMed","id":"10497236","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"10652302","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI16
Number of Residues	20
Details	Transmembrane: {"description":"Helical; Name=1","evidences":[{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI17
Number of Residues	41
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"14593096","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI18
Number of Residues	20
Details	Transmembrane: {"description":"Helical; Name=2","evidences":[{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI19
Number of Residues	38
Details	Topological domain: {"description":"Lumenal","evidences":[{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"14593096","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI20
Number of Residues	20
Details	Transmembrane: {"description":"Helical; Name=3","evidences":[{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI21
Number of Residues	20
Details	Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI22
Number of Residues	20
Details	Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI23
Number of Residues	20
Details	Transmembrane: {"description":"Helical; Name=6","evidences":[{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI24
Number of Residues	20
Details	Transmembrane: {"description":"Helical; Name=7","evidences":[{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI25
Number of Residues	18
Details	Intramembrane: {"description":"Helical","evidences":[{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI26
Number of Residues	20
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI27
Number of Residues	20
Details	Transmembrane: {"description":"Helical","evidences":[{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI28
Number of Residues	22
Details	Topological domain: {"description":"Lumenal","evidences":[{"source":"PubMed","id":"26280335","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12639958","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)