7XW3
Cryo-EM structure of an apo-form of human DICER
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000122 | biological_process | negative regulation of transcription by RNA polymerase II |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0003676 | molecular_function | nucleic acid binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0003725 | molecular_function | double-stranded RNA binding |
| A | 0004386 | molecular_function | helicase activity |
| A | 0004518 | molecular_function | nuclease activity |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0004521 | molecular_function | RNA endonuclease activity |
| A | 0004525 | molecular_function | ribonuclease III activity |
| A | 0004530 | molecular_function | deoxyribonuclease I activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006309 | biological_process | apoptotic DNA fragmentation |
| A | 0006396 | biological_process | RNA processing |
| A | 0010586 | biological_process | miRNA metabolic process |
| A | 0010626 | biological_process | negative regulation of Schwann cell proliferation |
| A | 0010629 | biological_process | negative regulation of gene expression |
| A | 0010804 | biological_process | negative regulation of tumor necrosis factor-mediated signaling pathway |
| A | 0014040 | biological_process | positive regulation of Schwann cell differentiation |
| A | 0016078 | biological_process | tRNA decay |
| A | 0016441 | biological_process | post-transcriptional gene silencing |
| A | 0016442 | cellular_component | RISC complex |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016891 | molecular_function | RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism |
| A | 0019904 | molecular_function | protein domain specific binding |
| A | 0021675 | biological_process | nerve development |
| A | 0030422 | biological_process | siRNA processing |
| A | 0031047 | biological_process | regulatory ncRNA-mediated gene silencing |
| A | 0031054 | biological_process | pre-miRNA processing |
| A | 0031643 | biological_process | positive regulation of myelination |
| A | 0032290 | biological_process | peripheral nervous system myelin formation |
| A | 0032720 | biological_process | negative regulation of tumor necrosis factor production |
| A | 0035196 | biological_process | miRNA processing |
| A | 0035197 | molecular_function | siRNA binding |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0048471 | cellular_component | perinuclear region of cytoplasm |
| A | 0048812 | biological_process | neuron projection morphogenesis |
| A | 0051239 | biological_process | regulation of multicellular organismal process |
| A | 0061980 | molecular_function | regulatory RNA binding |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0070578 | cellular_component | RISC-loading complex |
| A | 0070883 | molecular_function | pre-miRNA binding |
| A | 0070922 | biological_process | RISC complex assembly |
| A | 0098795 | biological_process | global gene silencing by mRNA cleavage |
Functional Information from PROSITE/UniProt
| site_id | PS00517 |
| Number of Residues | 9 |
| Details | RNASE_3_1 Ribonuclease III family signature. QRLEFLGDA |
| Chain | Residue | Details |
| A | GLN1702-ALA1710 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 176 |
| Details | Domain: {"description":"Helicase ATP-binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 169 |
| Details | Domain: {"description":"Helicase C-terminal","evidences":[{"source":"PROSITE-ProRule","id":"PRU00542","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 92 |
| Details | Domain: {"description":"Dicer dsRNA-binding fold","evidences":[{"source":"PROSITE-ProRule","id":"PRU00657","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 147 |
| Details | Domain: {"description":"PAZ","evidences":[{"source":"PROSITE-ProRule","id":"PRU00142","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 158 |
| Details | Domain: {"description":"RNase III 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00177","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 65 |
| Details | Domain: {"description":"DRBM","evidences":[{"source":"PROSITE-ProRule","id":"PRU00266","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 3 |
| Details | Motif: {"description":"DECH box"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 7 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 3 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"17920623","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2EB1","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | Site: {"description":"Important for activity","evidences":[{"source":"UniProtKB","id":"Q8R418","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
