7WTQ

Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Tsr1-2 (without Rps2)

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
CA	0000056	biological_process	ribosomal small subunit export from nucleus
CA	0000447	biological_process	endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
CA	0000472	biological_process	endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
CA	0003723	molecular_function	RNA binding
CA	0005634	cellular_component	nucleus
CA	0005730	cellular_component	nucleolus
CA	0005737	cellular_component	cytoplasm
CA	0030490	biological_process	maturation of SSU-rRNA
CA	0030686	cellular_component	90S preribosome
CA	0032040	cellular_component	small-subunit processome
CA	0042134	molecular_function	rRNA primary transcript binding
CA	0042254	biological_process	ribosome biogenesis
CA	0042255	biological_process	ribosome assembly
CA	0043248	biological_process	proteasome assembly
CA	0051082	molecular_function	unfolded protein binding
CB	0000056	biological_process	ribosomal small subunit export from nucleus
CB	0000447	biological_process	endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
CB	0005515	molecular_function	protein binding
CB	0005634	cellular_component	nucleus
CB	0005730	cellular_component	nucleolus
CB	0005737	cellular_component	cytoplasm
CB	0006364	biological_process	rRNA processing
CB	0008168	molecular_function	methyltransferase activity
CB	0008757	molecular_function	S-adenosylmethionine-dependent methyltransferase activity
CB	0016435	molecular_function	rRNA (guanine) methyltransferase activity
CB	0016740	molecular_function	transferase activity
CB	0032259	biological_process	methylation
CB	0043527	cellular_component	tRNA methyltransferase complex
CB	0070476	biological_process	rRNA (guanine-N7)-methylation
CC	0000461	biological_process	endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
CC	0000462	biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
CC	0000479	biological_process	endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
CC	0003723	molecular_function	RNA binding
CC	0003924	molecular_function	GTPase activity
CC	0005515	molecular_function	protein binding
CC	0005634	cellular_component	nucleus
CC	0005730	cellular_component	nucleolus
CC	0005737	cellular_component	cytoplasm
CC	0030686	cellular_component	90S preribosome
CC	0030688	cellular_component	preribosome, small subunit precursor
CC	0042254	biological_process	ribosome biogenesis
CC	0043021	molecular_function	ribonucleoprotein complex binding
SB	0000462	biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
SB	0002181	biological_process	cytoplasmic translation
SB	0003735	molecular_function	structural constituent of ribosome
SB	0005737	cellular_component	cytoplasm
SB	0005829	cellular_component	cytosol
SB	0005840	cellular_component	ribosome
SB	0006412	biological_process	translation
SB	0022627	cellular_component	cytosolic small ribosomal subunit
SB	0030686	cellular_component	90S preribosome
SB	1990904	cellular_component	ribonucleoprotein complex
SE	0002181	biological_process	cytoplasmic translation
SE	0003723	molecular_function	RNA binding
SE	0003735	molecular_function	structural constituent of ribosome
SE	0005737	cellular_component	cytoplasm
SE	0005829	cellular_component	cytosol
SE	0005840	cellular_component	ribosome
SE	0006412	biological_process	translation
SE	0019843	molecular_function	rRNA binding
SE	0022627	cellular_component	cytosolic small ribosomal subunit
SE	0030686	cellular_component	90S preribosome
SE	1990904	cellular_component	ribonucleoprotein complex
SG	0000462	biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
SG	0003735	molecular_function	structural constituent of ribosome
SG	0005654	cellular_component	nucleoplasm
SG	0005730	cellular_component	nucleolus
SG	0005737	cellular_component	cytoplasm
SG	0005829	cellular_component	cytosol
SG	0005840	cellular_component	ribosome
SG	0006364	biological_process	rRNA processing
SG	0006412	biological_process	translation
SG	0022627	cellular_component	cytosolic small ribosomal subunit
SG	0030686	cellular_component	90S preribosome
SG	0032040	cellular_component	small-subunit processome
SG	0042254	biological_process	ribosome biogenesis
SG	1990904	cellular_component	ribonucleoprotein complex
SH	0002181	biological_process	cytoplasmic translation
SH	0003735	molecular_function	structural constituent of ribosome
SH	0005654	cellular_component	nucleoplasm
SH	0005730	cellular_component	nucleolus
SH	0005737	cellular_component	cytoplasm
SH	0005829	cellular_component	cytosol
SH	0005840	cellular_component	ribosome
SH	0006364	biological_process	rRNA processing
SH	0006412	biological_process	translation
SH	0022627	cellular_component	cytosolic small ribosomal subunit
SH	0030686	cellular_component	90S preribosome
SH	0032040	cellular_component	small-subunit processome
SH	0042254	biological_process	ribosome biogenesis
SH	0042274	biological_process	ribosomal small subunit biogenesis
SH	1990904	cellular_component	ribonucleoprotein complex
SI	0000462	biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
SI	0003735	molecular_function	structural constituent of ribosome
SI	0005737	cellular_component	cytoplasm
SI	0005829	cellular_component	cytosol
SI	0005840	cellular_component	ribosome
SI	0006412	biological_process	translation
SI	0022627	cellular_component	cytosolic small ribosomal subunit
SI	0030686	cellular_component	90S preribosome
SI	1990904	cellular_component	ribonucleoprotein complex
SJ	0000462	biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
SJ	0003723	molecular_function	RNA binding
SJ	0003735	molecular_function	structural constituent of ribosome
SJ	0005515	molecular_function	protein binding
SJ	0005654	cellular_component	nucleoplasm
SJ	0005730	cellular_component	nucleolus
SJ	0005737	cellular_component	cytoplasm
SJ	0005829	cellular_component	cytosol
SJ	0005840	cellular_component	ribosome
SJ	0006364	biological_process	rRNA processing
SJ	0006412	biological_process	translation
SJ	0015935	cellular_component	small ribosomal subunit
SJ	0019843	molecular_function	rRNA binding
SJ	0022627	cellular_component	cytosolic small ribosomal subunit
SJ	0030476	biological_process	ascospore wall assembly
SJ	0030686	cellular_component	90S preribosome
SJ	0032040	cellular_component	small-subunit processome
SJ	0042254	biological_process	ribosome biogenesis
SJ	0042274	biological_process	ribosomal small subunit biogenesis
SJ	1990904	cellular_component	ribonucleoprotein complex
SL	0000028	biological_process	ribosomal small subunit assembly
SL	0000462	biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
SL	0003723	molecular_function	RNA binding
SL	0003735	molecular_function	structural constituent of ribosome
SL	0005737	cellular_component	cytoplasm
SL	0005829	cellular_component	cytosol
SL	0005840	cellular_component	ribosome
SL	0006412	biological_process	translation
SL	0019843	molecular_function	rRNA binding
SL	0022627	cellular_component	cytosolic small ribosomal subunit
SL	0030686	cellular_component	90S preribosome
SL	1990904	cellular_component	ribonucleoprotein complex
SN	0000462	biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
SN	0002181	biological_process	cytoplasmic translation
SN	0003735	molecular_function	structural constituent of ribosome
SN	0005730	cellular_component	nucleolus
SN	0005737	cellular_component	cytoplasm
SN	0005829	cellular_component	cytosol
SN	0005840	cellular_component	ribosome
SN	0006412	biological_process	translation
SN	0022627	cellular_component	cytosolic small ribosomal subunit
SN	0030686	cellular_component	90S preribosome
SN	0070181	molecular_function	small ribosomal subunit rRNA binding
SN	1990904	cellular_component	ribonucleoprotein complex
SO	0000028	biological_process	ribosomal small subunit assembly
SO	0000462	biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
SO	0002181	biological_process	cytoplasmic translation
SO	0003729	molecular_function	mRNA binding
SO	0003735	molecular_function	structural constituent of ribosome
SO	0005654	cellular_component	nucleoplasm
SO	0005730	cellular_component	nucleolus
SO	0005737	cellular_component	cytoplasm
SO	0005829	cellular_component	cytosol
SO	0005840	cellular_component	ribosome
SO	0006364	biological_process	rRNA processing
SO	0006412	biological_process	translation
SO	0022627	cellular_component	cytosolic small ribosomal subunit
SO	0030686	cellular_component	90S preribosome
SO	0032040	cellular_component	small-subunit processome
SO	0042254	biological_process	ribosome biogenesis
SO	1990904	cellular_component	ribonucleoprotein complex
SW	0002181	biological_process	cytoplasmic translation
SW	0003735	molecular_function	structural constituent of ribosome
SW	0005737	cellular_component	cytoplasm
SW	0005829	cellular_component	cytosol
SW	0005840	cellular_component	ribosome
SW	0006412	biological_process	translation
SW	0022627	cellular_component	cytosolic small ribosomal subunit
SW	1990904	cellular_component	ribonucleoprotein complex
SX	0000462	biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
SX	0003735	molecular_function	structural constituent of ribosome
SX	0005737	cellular_component	cytoplasm
SX	0005829	cellular_component	cytosol
SX	0005840	cellular_component	ribosome
SX	0006412	biological_process	translation
SX	0006450	biological_process	regulation of translational fidelity
SX	0015935	cellular_component	small ribosomal subunit
SX	0022627	cellular_component	cytosolic small ribosomal subunit
SX	1990145	biological_process	maintenance of translational fidelity
SX	1990904	cellular_component	ribonucleoprotein complex
SY	0000462	biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
SY	0002181	biological_process	cytoplasmic translation
SY	0003735	molecular_function	structural constituent of ribosome
SY	0005737	cellular_component	cytoplasm
SY	0005739	cellular_component	mitochondrion
SY	0005829	cellular_component	cytosol
SY	0005840	cellular_component	ribosome
SY	0006412	biological_process	translation
SY	0022627	cellular_component	cytosolic small ribosomal subunit
SY	1990904	cellular_component	ribonucleoprotein complex
Sb	0000028	biological_process	ribosomal small subunit assembly
Sb	0000462	biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
Sb	0003723	molecular_function	RNA binding
Sb	0003735	molecular_function	structural constituent of ribosome
Sb	0005737	cellular_component	cytoplasm
Sb	0005829	cellular_component	cytosol
Sb	0005840	cellular_component	ribosome
Sb	0006412	biological_process	translation
Sb	0008270	molecular_function	zinc ion binding
Sb	0022627	cellular_component	cytosolic small ribosomal subunit
Sb	0046872	molecular_function	metal ion binding
Sb	1990904	cellular_component	ribonucleoprotein complex
Se	0002181	biological_process	cytoplasmic translation
Se	0003735	molecular_function	structural constituent of ribosome
Se	0005737	cellular_component	cytoplasm
Se	0005829	cellular_component	cytosol
Se	0005840	cellular_component	ribosome
Se	0006412	biological_process	translation
Se	0006451	biological_process	translational readthrough
Se	0010494	cellular_component	cytoplasmic stress granule
Se	0022627	cellular_component	cytosolic small ribosomal subunit
Se	1990904	cellular_component	ribonucleoprotein complex

Functional Information from PROSITE/UniProt

site_id	PS00053
Number of Residues	18
Details	RIBOSOMAL_S8 Ribosomal protein S8 signature. GyvILTTSaGIMdheeAR

Chain	Residue	Details
SW	GLY100-ARG117

---

site_id	PS00054
Number of Residues	23
Details	RIBOSOMAL_S11 Ribosomal protein S11 signature. LrALarS.GLrIgrieDvTPvPSD

Chain	Residue	Details
SO	LEU102-ASP124

---

site_id	PS00055
Number of Residues	8
Details	RIBOSOMAL_S12 Ribosomal protein S12 signature. KqPNSAiR

Chain	Residue	Details
SX	LYS62-ARG69

---

site_id	PS00056
Number of Residues	13
Details	RIBOSOMAL_S17 Ribosomal protein S17 signature. GDiVtVgQcRPIS

Chain	Residue	Details
SL	GLY120-SER132

---

site_id	PS00362
Number of Residues	31
Details	RIBOSOMAL_S15 Ribosomal protein S15 signature. VrkHLernRkDkdAkfrLilIesrihrLarY

Chain	Residue	Details
SN	VAL98-TYR128

---

site_id	PS00528
Number of Residues	15
Details	RIBOSOMAL_S4E Ribosomal protein S4e signature. HlKRLAaPhhWlLdK

Chain	Residue	Details
SE	HIS8-LYS22

---

site_id	PS00529
Number of Residues	23
Details	RIBOSOMAL_S24E Ribosomal protein S24e signature. FGggKSvGFGlVYNSvaeakkfE

Chain	Residue	Details
SY	PHE64-GLU86

---

site_id	PS00578
Number of Residues	12
Details	RIBOSOMAL_S6E Ribosomal protein S6e signature. ISGGnDkqGfPM

Chain	Residue	Details
SG	ILE52-MET63

---

site_id	PS00632
Number of Residues	25
Details	RIBOSOMAL_S4 Ribosomal protein S4 signature. LErRLqtqVYKlglAkSvhhARvLI

Chain	Residue	Details
SJ	LEU105-ILE129

---

site_id	PS00948
Number of Residues	14
Details	RIBOSOMAL_S7E Ribosomal protein S7e signature. KLtRELEKKFQdRH

Chain	Residue	Details
SH	LYS76-HIS89

---

site_id	PS01168
Number of Residues	33
Details	RIBOSOMAL_S27E Ribosomal protein S27e signature. KCpgClnittvFShAqtavtCesCStiLctPtG

Chain	Residue	Details
Sb	LYS36-GLY68

---

site_id	PS01191
Number of Residues	13
Details	RIBOSOMAL_S3AE Ribosomal protein S3Ae signature. LkGRVvEvCLaDL

Chain	Residue	Details
SB	LEU61-LEU73

---

site_id	PS01193
Number of Residues	20
Details	RIBOSOMAL_S8E Ribosomal protein S8e signature. RsaTGAkraqf.RkKrKfELG

Chain	Residue	Details
SI	ARG11-GLY30

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	63
Details	Domain: {"description":"S4 RNA-binding"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"17330950","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"17287358","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"15665377","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	33
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"22106047","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"30609991","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"19779198","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"17287358","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"17287358","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19779198","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	5
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"19779198","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"18407956","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	3
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18407956","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	74
Details	Domain: {"description":"S4 RNA-binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00182","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P05755","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"P05755","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	2
Details	Modified residue: {"description":"N-acetylserine","evidences":[{"source":"PubMed","id":"10601260","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"1544921","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	72
Details	Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	30
Details	Compositional bias: {"description":"Basic residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	1
Details	Modified residue: {"description":"3,4-dihydroxyproline","evidences":[{"source":"PubMed","id":"24550462","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI20
Number of Residues	22
Details	Zinc finger: {"description":"C4-type","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI21
Number of Residues	1
Details	Modified residue: {"description":"S-methylcysteine","evidences":[{"source":"PubMed","id":"22650761","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI22
Number of Residues	52
Details	Domain: {"description":"KH"}

Chain

Residue

Details

---

site_id	SWS_FT_FI23
Number of Residues	7
Details	Motif: {"description":"Nuclear localization signal","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI24
Number of Residues	166
Details	Domain: {"description":"Bms1-type G","evidences":[{"source":"PROSITE-ProRule","id":"PRU01051","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---